104 research outputs found
Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaea : research article
Background Differential expression of genes can be regulated on many different levels. Most global studies of gene regulation concentrate on transcript level regulation, and very few global analyses of differential translational efficiencies exist. The studies have revealed that in Saccharomyces cerevisiae, Arabidopsis thaliana, and human cell lines translational regulation plays a significant role. Additional species have not been investigated yet. Particularly, until now no global study of translational control with any prokaryotic species was available. Results A global analysis of translational control was performed with two haloarchaeal model species, Halobacterium salinarum and Haloferax volcanii. To identify differentially regulated genes, exponentially growing and stationary phase cells were compared. More than 20% of H. salinarum transcripts are translated with non-average efficiencies. By far the largest group is comprised of genes that are translated with above-average efficiency specifically in exponential phase, including genes for many ribosomal proteins, RNA polymerase subunits, enzymes, and chemotaxis proteins. Translation of 1% of all genes is specifically repressed in either of the two growth phases. For comparison, DNA microarrays were also used to identify differential transcriptional regulation in H. salinarum, and 17% of all genes were found to have non-average transcript levels in exponential versus stationary phase. In H. volcanii, 12% of all genes are translated with non-average efficiencies. The overlap with H. salinarum is negligible. In contrast to H. salinarum, 4.6% of genes have non-average translational efficiency in both growth phases, and thus they might be regulated by other stimuli than growth phase. Conclusions For the first time in any prokaryotic species it was shown that a significant fraction of genes is under differential translational control. Groups of genes with different regulatory patterns were discovered. However, neither the fractions nor the identity of regulated genes are conserved between H. salinarum and H. volcanii, indicating that prokaryotes as well as eukaryotes use differential translational control for the regulation of gene expression, but that the identity of regulated genes is not conserved For 70 H. salinarum genes potentiation of regulation was observed, but for the majority of regulated genes either transcriptional or translational regulation is employed
Discrimination between static and dynamic heterogeneities in single dye diffusion in ultrathin liquid films
Investigations of static and dynamic heterogeneities in ultra-thin liquid films via scaled squared displacements of single molecule diffusion
Investigations of static and dynamic heterogeneities in ultra-thin liquid films via scaled squared displacements of single molecule diffusion
Discrimination between static and dynamic heterogeneities in single dye diffusion in ultrathin liquid films
Die schweizerische Agenda für klinische Pflegeforschung von 2007 bis 2017 : der Stand der Umsetzung
Eine Forschungsagenda fasst relevante Fragestellungen der Forschung zu einem bestimmten Thema zusammen und zeigt wichtige Handlungsfelder auf. Der Schweizerische Verein für Pflegewissenschaft (VFP) setzte sich zum Ziel, die Entwicklung der Pflegewissenschaft und damit verbunden die Forschung in der Schweiz zu fördern und zu unterstützen. Daniela Händler-Schuster und ihr Autorenteam stellen im Folgenden die Entwicklung und die derzeitige Umsetzung der Swiss Research Agenda in Nursing vor
Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaea
<p>Abstract</p> <p>Background</p> <p>Differential expression of genes can be regulated on many different levels. Most global studies of gene regulation concentrate on transcript level regulation, and very few global analyses of differential translational efficiencies exist. The studies have revealed that in <it>Saccharomyces cerevisiae</it>, <it>Arabidopsis thaliana</it>, and human cell lines translational regulation plays a significant role. Additional species have not been investigated yet. Particularly, until now no global study of translational control with any prokaryotic species was available.</p> <p>Results</p> <p>A global analysis of translational control was performed with two haloarchaeal model species, <it>Halobacterium salinarum </it>and <it>Haloferax volcanii</it>. To identify differentially regulated genes, exponentially growing and stationary phase cells were compared.</p> <p>More than 20% of <it>H. salinarum </it>transcripts are translated with non-average efficiencies. By far the largest group is comprised of genes that are translated with above-average efficiency specifically in exponential phase, including genes for many ribosomal proteins, RNA polymerase subunits, enzymes, and chemotaxis proteins. Translation of 1% of all genes is specifically repressed in either of the two growth phases. For comparison, DNA microarrays were also used to identify differential transcriptional regulation in <it>H. salinarum</it>, and 17% of all genes were found to have non-average transcript levels in exponential versus stationary phase.</p> <p>In <it>H. volcanii</it>, 12% of all genes are translated with non-average efficiencies. The overlap with <it>H. salinarum </it>is negligible. In contrast to <it>H. salinarum</it>, 4.6% of genes have non-average translational efficiency in both growth phases, and thus they might be regulated by other stimuli than growth phase.</p> <p>Conclusion</p> <p>For the first time in any prokaryotic species it was shown that a significant fraction of genes is under differential translational control. Groups of genes with different regulatory patterns were discovered. However, neither the fractions nor the identity of regulated genes are conserved between <it>H. salinarum </it>and <it>H. volcanii</it>, indicating that prokaryotes as well as eukaryotes use differential translational control for the regulation of gene expression, but that the identity of regulated genes is not conserved.</p> <p>For 70 <it>H. salinarum </it>genes potentiation of regulation was observed, but for the majority of regulated genes either transcriptional or translational regulation is employed.</p
The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes
The rimb1 (redox imbalanced 1) mutation was mapped to the RCD1 locus (radical-
induced cell death 1; At1g32230) demonstrating that a major factor involved in
redox-regulation genes for chloroplast antioxidant enzymes and protection
against photooxidative stress, RIMB1, is identical to the regulator of disease
response reactions and cell death, RCD1. Discovering this link let to our
investigation of its regulatory mechanism. We show in yeast that RCD1 can
physically interact with the transcription factor Rap2.4a which provides
redox-sensitivity to nuclear expression of genes for chloroplast antioxidant
enzymes. In the rimb1 (rcd1-6) mutant, a single nucleotide exchange results in
a truncated RCD1 protein lacking the transcription factor binding site.
Protein-protein interaction between full-length RCD1 and Rap2.4a is supported
by H2O2, but not sensitive to the antioxidants dithiotreitol and ascorbate. In
combination with transcript abundance analysis in Arabidopsis, it is concluded
that RCD1 stabilizes the Rap2.4-dependent redox-regulation of the genes
encoding chloroplast antioxidant enzymes in a widely redox-independent manner.
Over the years, rcd1-mutant alleles have been described to develop symptoms
like chlorosis, lesions along the leaf rims and in the mesophyll and
(secondary) induction of extra- and intra-plastidic antioxidant defense
mechanisms. All these rcd1 mutant characteristics were observed in rcd1-6 to
succeed low activation of the chloroplast antioxidant system and glutathione
biosynthesis. We conclude that RCD1 protects plant cells from running into
reactive oxygen species (ROS)-triggered programs, such as cell death and
activation of pathogen-responsive genes (PR genes) and extra-plastidic
antioxidant enzymes, by supporting the induction of the chloroplast
antioxidant system
Synthesis of ultradisperse carbon dioxide powder with plasma-dynamic method in the coaxial magneto-plasma accelerator
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