The X-ray Spectral Properties and Variability of Luminous High-Redshift Active Galactic Nuclei

We perform a detailed investigation of moderate-to-high quality X-ray spectra of ten of the most luminous active galactic nuclei (AGNs) known at z>4 (up to z~6.28). This study includes five new XMM observations and five archived X-ray observations (four by XMM and one by Chandra). We find that the X-ray power-law photon indices of our sample, composed of eight radio-quiet sources and two that are moderately radio loud, are not significantly different from those of lower redshift AGNs. The upper limits obtained on intrinsic neutral hydrogen column densities, N_H<~10^{22}-10^{23} cm^{-2}, indicate that these AGNs are not significantly absorbed. A joint fit performed on our eight radio-quiet sources, with a total of ~7000 photons, constrains the mean photon index of z>4 radio-quiet AGNs to Gamma=1.97^{+0.06}_{-0.04}, with no detectable intrinsic dispersion from source to source. We also obtain a strong constraint on the mean intrinsic column density, N_H<~3x10^{21} cm^{-2}, showing that optically selected radio-quiet AGNs at z>4 are, on average, not more absorbed than their lower-redshift counterparts. All this suggests that the X-ray production mechanism and the central environment in radio-quiet AGNs have not significantly evolved over cosmic time. The mean equivalent width of a putative neutral narrow Fe Ka line is constrained to be <~190 eV, and similarly we place constraints on the mean Compton reflection component (R<~1.2). None of the AGNs varied on short (~1 hr) timescales, but on longer timescales (months-to-years) strong variability is observed in four of the sources. In particular, the X-ray flux of the z=5.41 radio-quiet AGN SDSS 0231-0728 dropped by a factor of ~4 over a rest-frame period of 73 d. This is the most extreme X-ray variation observed in a luminous z>4 radio-quiet AGN.Comment: 10 pages (emulateapj), 5 figures. Accepted by Ap

SPHERES, J\"ulich's High-Flux Neutron Backscattering Spectrometer at FRM II

SPHERES (SPectrometer with High Energy RESolution) is a third-generation neutron backscattering spectrometer, located at the 20 MW German neutron source FRM II and operated by the Juelich Centre for Neutron Science. It offers an energy resolution (fwhm) better than 0.65 micro-eV, a dynamic range of +-31 micro-eV, and a signal-to-noise ratio of up to 1750:1.Comment: 12 pages, 7 figures, 2 tables. Supplemental material consists of 3 pages, 2 figures, 2 table

Sensitive observations at 1.4 and 250 GHz of z > 5 QSOs

We present 1.4 and 5 GHz observations taken with the Very Large Array (VLA), and observations at 250 GHz obtained with the Max-Planck millimeter bolometer (MAMBO) at the IRAM 30~m telescope, of ten optically selected Quasi-stellar Objects (QSOs) at 5.0 < z < 6.28. Four sources are detected at 1.4 GHz two of which are radio loud and are also detected at 5 GHz. These results are roughly consistent with there being no evolution of the radio-loud QSO fraction out to z~6. Three sources have been detected at 250 GHz or 350 GHz at much higher levels than their 1.4 GHz flux densities suggesting that the observed mm emission is likely thermal emission from warm dust, although more exotic possibilities cannot be precluded. The highest redshift source in our sample (J1030+0524 at z=6.28) is not detected at 1.4 or 250 GHz, but four fairly bright radio sources (flux density at 1.4GHz > 0.2 mJy) are detected in a 2' field centered on the QSO, including an edge-brightened ('FRII') double radio source with an extent of about 1'. A similar over-density of radio sources is seen in the field of the highest redshift QSO J1148+5251. We speculate that these over-densities of radio sources may indicate clusters along the lines-of-sight, in which case gravitational lensing by the cluster could magnify the QSO emission by a factor 2 or so without giving rise to arcsecond-scale distortions in the optical images of the QSOs.Comment: 25 pages, 12 figures. accepted by A

Effects of aldosterone on the human placenta: Insights from placental perfusion studies.

INTRODUCTION In pregnancy, aldosterone is linked to maternal plasma volume expansion, improved fetal and placental growth/angiogenesis and reduced maternal blood pressure. Aldosterone levels are low in women with pre-eclampsia. Given the placental growth properties of aldosterone in pregnancy, we hypothesised that increased aldosterone improves placental function ex vivo. We applied aldosterone in the dual human placenta perfusion model and analysed specific regulatory markers. METHODS A single cotyledon was perfused using a trimodal perfusion setup consisting of a control phase (CP; basic perfusion medium (BPM) alone) and two consecutive experimental phases (EP1/EP2; BPM supplemented with 1.5 x 10-9M and 1.5 x 10-7M aldosterone, respectively). CP and EP1/EP2 were conducted in closed circuits lasting 2 h each. Quality/time control perfusions using BPM alone were performed for 360 min to distinguish time-dependent effects from aldosterone-related effects. Perfusates were assessed for control parameters (pH/pO2/pCO2/glucose/lactate/creatinine/antipyrine). Maternal perfusates were analysed for placental growth factor (PlGF), soluble fms-like tyrosine kinase-1 (sFlt-1), interleukin-10 (IL-10) and tumour necrosis factor-alpha (TNF-α) using ELISAs. mRNA expression of abovementioned factors was measured by qPCR in post-perfusion tissue. RESULTS Data from quality/time control perfusions indicated that TNF-α and IL-10 release continuously increased over time. Contrary, in the trimodal perfusion setup the application of aldosterone decreased TNF-α secretion (P < 0.05, EP1/EP2 vs CP, 120 min) and increased PlGF release (P < 0.05, EP1 vs CP, 90/120 min) into the maternal perfusates. mRNA expression followed similar trends, but did not reach significance. DISCUSSION Our ex vivo placental perfusion data suggest that increasing aldosterone promotes anti-inflammatory and pro-angiogenic factors, which could positively contribute to healthy pregnancy outcomes

Four-shooter: a large format charge-coupled-device camera for the Hale telescope

We describe an astronomical camera for the 200-in. Hale telescope using four 800 X800 Texas Instruments CCDs in an optical arrangement that allows imaging of a contiguous 1600-pixel-square region of sky. The system employs reimaging optics to yield a scale of 0.33 arcsec per pixel, a good match to the best seeing conditions at Palomar Observatory. Modern high-efficiency coatings are used in the complex optical system to yield a throughput at peak efficiency of nearly 50% (including the losses in the telescope), corresponding to a quantum efficiency on the sky of about 30%. The system uses a fifth CCD in a spectroscopic channel, and it is possible to obtain simultaneous imaging and spectroscopic observations with the system. The camera may also be used in a scanning mode, in which the telescope tracking rate is offset, and the charge is clocked in the chips in such a manner as to keep the charge image aligned with the optical image. In this way, a survey for high-redshift quasars has been carried out over a large area of sky. The instrument has produced images for the most distant clusters of galaxies yet discovered as well as spectra of the most distant galaxies yet observed

The Cluster Mass Function from Early SDSS Data: Cosmological Implications

The mass function of clusters of galaxies is determined from 400 deg^2 of early commissioning imaging data of the Sloan Digital Sky Survey; ~300 clusters in the redshift range z = 0.1 - 0.2 are used. Clusters are selected using two independent selection methods: a Matched Filter and a red-sequence color magnitude technique. The two methods yield consistent results. The cluster mass function is compared with large-scale cosmological simulations. We find a best-fit cluster normalization relation of sigma_8*omega_m^0.6 = 0.33 +- 0.03 (for 0.1 ~< omega_m ~< 0.4), or equivalently sigma_8 = (0.16/omega_m)^0.6. The amplitude of this relation is significantly lower than the previous canonical value, implying that either omega_m is lower than previously expected (omega_m = 0.16 if sigma_8 = 1) or sigma_8 is lower than expected (sigma_8 = 0.7 if omega_m = 0.3). The best-fit mass function parameters are omega_m = 0.19 (+0.08,-0.07) and sigma_8 = 0.9 (+0.3,-0.2). High values of omega_m (>= 0.4) and low sigma_8 (=~ 2 sigma.Comment: AASTeX, 25 pages, including 7 figures, accepted for publication in ApJ, vol.585, March 200

The coactivator role of histone deacetylase 3 in IL-1-signaling involves deacetylation of p65 NF-κB

Histone deacetylase (HDAC) 3, as a cofactor in co-repressor complexes containing silencing mediator for retinoid or thyroid-hormone receptors (SMRT) and nuclear receptor co-repressor (N-CoR), has been shown to repress gene transcription in a variety of contexts. Here, we reveal a novel role for HDAC3 as a positive regulator of IL-1-induced gene expression. Various experimental approaches involving RNAi-mediated knockdown, conditional gene deletion or small molecule inhibitors indicate a positive role of HDAC3 for transcription of the majority of IL-1-induced human or murine genes. This effect was independent from the gene regulatory effects mediated by the broad-spectrum HDAC inhibitor trichostatin A (TSA) and thus suggests IL-1-specific functions for HDAC3. The stimulatory function of HDAC3 for inflammatory gene expression involves a mechanism that uses binding to NF-κB p65 and its deacetylation at various lysines. NF-κB p65-deficient cells stably reconstituted to express acetylation mimicking forms of p65 (p65 K/Q) had largely lost their potential to stimulate IL-1-triggered gene expression, implying that the co-activating property of HDAC3 involves the removal of inhibitory NF-κB p65 acetylations at K122, 123, 314 and 315. These data describe a novel function for HDAC3 as a co-activator in inflammatory signaling pathways and help to explain the anti-inflammatory effects frequently observed for HDAC inhibitors in (pre)clinical us

The coactivator role of histone deacetylase 3 in IL-1-signaling involves deacetylation of p65 NF-kappaB

Histone deacetylase (HDAC) 3, as a cofactor in co-repressor complexes containing silencing mediator for retinoid or thyroid-hormone receptors (SMRT) and nuclear receptor co-repressor (N-CoR), has been shown to repress gene transcription in a variety of contexts. Here, we reveal a novel role for HDAC3 as a positive regulator of IL-1-induced gene expression. Various experimental approaches involving RNAi-mediated knockdown, conditional gene deletion or small molecule inhibitors indicate a positive role of HDAC3 for transcription of the majority of IL-1-induced human or murine genes. This effect was independent from the gene regulatory effects mediated by the broad-spectrum HDAC inhibitor trichostatin A (TSA) and thus suggests IL-1-specific functions for HDAC3. The stimulatory function of HDAC3 for inflammatory gene expression involves a mechanism that uses binding to NF-?B p65 and its deacetylation at various lysines. NF-?B p65-deficient cells stably reconstituted to express acetylation mimicking forms of p65 (p65 K/Q) had largely lost their potential to stimulate IL-1-triggered gene expression, implying that the co-activating property of HDAC3 involves the removal of inhibitory NF-?B p65 acetylations at K122, 123, 314 and 315. These data describe a novel function for HDAC3 as a co-activator in inflammatory signaling pathways and help to explain the anti-inflammatory effects frequently observed for HDAC inhibitors in (pre)clinical use