Evaluation of the sleep project for unaccompanied asylum-seeking children in Kent

It has been a privilege to evaluate the Sleep Project intervention for unaccompanied asylum-seeking children (UASC). The opportunity to evaluate this project arose through discussions between the authors and Dr. Ana Draper, exploring the work of Ana, her team and colleagues across the various agencies in supporting newly-arrived migrant children in Kent. From 2015, there was a rapid increase in the number of UASC arriving into the region and services were quickly adapted to meet the specific and immediate needs of these vulnerable children and young people, the Sleep Project being just one of the innovative interventions put in place. Unaccompanied asylum-seeking children and young people have usually experienced harrowing journeys to the United Kingdom (UK) in seeking safety and refuge. Once in the UK, adapting to life within reception centres, foster families or supported housing, brings further challenges and within this context, practitioners and the young people identified sleep as a key problematic issue for which they required extra support. Through conversations with practitioners and young people, sleep difficulties were a recurring issue. Lack of sleep and disturbed sleep was preventing the young people from engaging in planned activities such as language classes. Tiredness was having negative health and social/educational impacts. This evaluation studies the benefits and challenges of the creative support mechanisms that were developed to address the sleep issues. This report presents our findings from the evaluation study of the Sleep Project intervention. The study comprised of 18 interviews with practitioners either working directly or indirectly with UASC, in paid and voluntary capacities. From the interviews, the qualitative data was thematically analysed to develop themes under which the benefits and challenges of the intervention could be explored. Throughout the interviews with practitioners working either directly with UASC or indirectly in managerial roles, it became apparent that there was a high level of commitment from individuals to develop their understanding of UASCs’ needs and to develop appropriate social care practice and support. The interviews highlighted that practitioners were prepared to think and act creatively to improve and to tailor support for this group of children and young people. The findings of the evaluation suggest that the Sleep Project was very well-received by young people and practitioners alike. It provided practical resources and support for good sleep, and it encouraged conversations to develop between the practitioners and the young people, and between the young people themselves, normalising the sleep issues that they were experiencing, and, according to interviewees, the young people were found to be encouraging other young people to use the good sleep packs. The intervention helped the practitioners feel more confident and equipped with skills to talk to the young people about sleep and, possibly, this led to deeper discussions about individual journeys and experiences, allowing care to become more empathetic, specific and person-centred. Significantly, interviewees reported that the project allowed them to ‘look at the basics’, that is, practical help such as providing night lights and educating young people about factors that hamper a good night’s sleep, whilst practitioners gained a greater understanding and responsiveness as to why the young people could struggle with sleep. This greater understanding has been important for shifting the perceptions of practitioners, particularly those in educational roles, helping them to be more patient and supportive to young people struggling to get to lessons on time and to concentrate. Key messages from the findings of this evaluation study are encapsulated in the following quotes from interviewees: • ‘I think it’s thinking a bit more innovatively about the care we can provide’ • ‘A confidence to look at the basics’ • ‘Context switched concepts’. Proposed recommendations involve: sustaining the work so far, looking at how the project could/should have a legacy, and building on the developed knowledge and networks. At the time of the publication of this report, young people are being transferred to other receiving local authorities outside Kent – a national dispersal scheme that was agreed by the Home Office in June 2016 to ease the pressure on Kent - therefore good practice from this project should be widely disseminated to service providers and policy makers at regional and national levels

College-aged women in the United States that play overhand throwing sports have masculine digit ratios

Athletic prowess in both males and females is negatively correlated with the ratio between the lengths of the second and fourth fingers (2D:4D), a correlate of prenatal testosterone exposure. Because multiple lines of evidence suggest that prenatal testosterone exposure is associated with sports interest, motivation, and athletic performance we measured the digit ratios of 77 non-athletes, 103 varsity athletes, and 78 club sport athletes to test 8 hypotheses about the relationship between digit ratio and the athletic behavior of collegeage women in the USA. Using independent samples t-tests, we found no significant differences between the digit ratios of women that (1) were athletes and non-athletes, (2) were varsity or club sport athletes, (3) had played or were currently playing individual or team sports, (4) played contact and non-contact sports, (5) played sports involving a ball and those that do not, (6) played sports where the outcome was determined by a score or the outcome of direct physical competition or subjectively by judges, or (7) were starters or reserves on their teams. However, women that played overhand throwing sports softball and water polo had significantly smaller digit ratios than did women that played other sports. These differences were not due to scaling effects. The independent samples t-test results were supported by subsequent Monte Carlo bootstrap, Bayesian, Random Forest, and multiple linear regression analyses. We suggest that the organizational consequences of prenatal testosterone exposure may influence the anatomy and physiology of women that leads to success playing overhand throwing sports

Austerity and the lives of people with learning disabilities. A thematic synthesis of current literature

The Financial Crisis of 2008 resulted in many western economies implementing cuts in health and social care. This systematic review provides a holistic picture of the impact of austerity policy on the lives of people with learning disabilities (LD) and the collateral effects on the people who support them. Our review suggests that in the current climate of economic austerity, available funding to support people with LD is no longer aligned to their care needs. Cuts in disability services have adversely affected the well-being both of people with LD and their informal carers. Individuals with LD have lost social support and are experiencing increased social isolation. Heightened demands on family carers’ time have negatively influenced their wider roles, including parental functioning, and labour market participation. Our review provides the foundations for further discourse and research on the effects of austerity on people with LD and their family carers

Infection pre-Ad26.COV2.S-vaccination primes greater class switching and reduced CXCR5 expression by SARS-CoV-2-specific memory B cells

Neutralizing antibodies strongly correlate with protection for COVID-19 vaccines, but the corresponding memory B cells that form to protect against future infection are relatively understudied. Here we examine the effect of prior SARS-CoV-2 infection on the magnitude and phenotype of the memory B cell response to single dose Johnson and Johnson (Ad26.COV2.S) vaccination in South African health care workers. Participants were either naïve to SARS-CoV-2 or had been infected before vaccination. SARS-CoV-2-specific memory B-cells expand in response to Ad26.COV2.S and are maintained for the study duration (84 days) in all individuals. However, prior infection is associated with a greater frequency of these cells, a significant reduction in expression of the germinal center chemokine receptor CXCR5, and increased class switching. These B cell features correlated with neutralization and antibody-dependent cytotoxicity (ADCC) activity, and with the frequency of SARS-CoV-2 specific circulating T follicular helper cells (cTfh). Vaccination-induced effective neutralization of the D614G variant in both infected and naïve participants but boosted neutralizing antibodies against the Beta and Omicron variants only in participants with prior infection. In addition, the SARS-CoV-2 specific CD8+ T cell response correlated with increased memory B cell expression of the lung-homing receptor CXCR3, which was sustained in the previously infected group. Finally, although vaccination achieved equivalent B cell activation regardless of infection history, it was negatively impacted by age. These data show that phenotyping the response to vaccination can provide insight into the impact of prior infection on memory B cell homing, CSM, cTfh, and neutralization activity. These data can provide early signals to inform studies of vaccine boosting, durability, and co-morbidities

Intracranial V. cholerae Sialidase Protects against Excitotoxic Neurodegeneration

Converging evidence shows that GD3 ganglioside is a critical effector in a number of apoptotic pathways, and GM1 ganglioside has neuroprotective and noötropic properties. Targeted deletion of GD3 synthase (GD3S) eliminates GD3 and increases GM1 levels. Primary neurons from GD3S−/− mice are resistant to neurotoxicity induced by amyloid-β or hyperhomocysteinemia, and when GD3S is eliminated in the APP/PSEN1 double-transgenic model of Alzheimer's disease the plaque-associated oxidative stress and inflammatory response are absent. To date, no small-molecule inhibitor of GD3S exists. In the present study we used sialidase from Vibrio cholerae (VCS) to produce a brain ganglioside profile that approximates that of GD3S deletion. VCS hydrolyzes GD1a and complex b-series gangliosides to GM1, and the apoptogenic GD3 is degraded. VCS was infused by osmotic minipump into the dorsal third ventricle in mice over a 4-week period. Sensorimotor behaviors, anxiety, and cognition were unaffected in VCS-treated mice. To determine whether VCS was neuroprotective in vivo, we injected kainic acid on the 25th day of infusion to induce status epilepticus. Kainic acid induced a robust lesion of the CA3 hippocampal subfield in aCSF-treated controls. In contrast, all hippocampal regions in VCS-treated mice were largely intact. VCS did not protect against seizures. These results demonstrate that strategic degradation of complex gangliosides and GD3 can be used to achieve neuroprotection without adversely affecting behavior

Prostaglandin E2 Reverses Aberrant Production of an Inflammatory Chemokine by Microglia from Sandhoff Disease Model Mice through the cAMP-PKA Pathway

Background: Sandhoff disease (SD) is a neurodegenerative lysosomal b-hexosaminidase (Hex) deficiency involving excessive accumulation of undegraded substrates, including terminal GlcNAc-oligosaccharides and GM2 ganglioside. Microglia-mediated neuroinflammation contributes to the pathogenesis and progression of SD. Our previous study demonstrated that MIP-1a, a putative pathogenic factor for SD, is up-regulated in microglial cells derived from SD model mice (SD-Mg) through activation of Akt and JNK. Methodology/Principal Findings: In this study, we first demonstrated that prostaglandin E2 (PGE2), which is one of the lipid mediators derived from arachidonic acid and is known to suppress activation of microglia, reduced the aberrant MIP-1a production by SD-Mg to the same level as by WT-Mg. PGE2 also attenuated the activation of Akt and JNK. The inhibition of MIP-1a production and the activation of Akt and JNK occurred through the EP2 and 4/cAMP/PKA signaling pathway in the murine microglia derived from SD model mice. Conclusions/Significance: We propose that PGE2 plays a role as a negative regulator of MIP-1a production in th

Humanized Rag1−/−γc−/− Mice Support Multilineage Hematopoiesis and Are Susceptible to HIV-1 Infection via Systemic and Vaginal Routes

Several new immunodeficient mouse models for human cell engraftment have recently been introduced that include the Rag2−/−γc−/−, NOD/SCID, NOD/SCIDγc−/− and NOD/SCIDβ2m−/− strains. Transplantation of these mice with CD34+ human hematopoietic stem cells leads to prolonged engraftment, multilineage hematopoiesis and the capacity to generate human immune responses against a variety of antigens. However, the various mouse strains used and different methods of engrafting human cells are beginning to illustrate strain specific variations in engraftment levels, duration and longevity of mouse life span. In these proof-of-concept studies we evaluated the Balb/c-Rag1−/−γ−/− strain for engraftment by human fetal liver derived CD34+ hematopoietic cells using the same protocol found to be effective for Balb/c-Rag2−/−γc−/− mice. We demonstrate that these mice can be efficiently engrafted and show multilineage human hematopoiesis with human cells populating different lymphoid organs. Generation of human cells continues beyond a year and production of human immunoglobulins is noted. Infection with HIV-1 leads to chronic viremia with a resultant CD4 T cell loss. To mimic the predominant sexual viral transmission, we challenged humanized Rag1−/−γc−/− mice with HIV-1 via vaginal route which also resulted in chronic viremia and helper T cell loss. Thus these mice can be further exploited for studying human pathogens that infect the human hematopoietic system in an in vivo setting

The Prospects for Payment for Ecosystem Services (PES) in Vietnam: A Look at Three Payment Schemes

Global conservation discourses and practices increasingly rely on market-based solutions to fulfill the dual objective of forest conservation and economic development. Although varied, these interventions are premised on the assumption that natural resources are most effectively managed and preserved while benefiting livelihoods if the market-incentives of a liberalised economy are correctly in place. By examining three nationally supported payment for ecosystem service (PES) schemes in Vietnam we show how insecure land tenure, high transaction costs and high opportunity costs can undermine the long-term benefits of PES programmes for local households and, hence, potentially threaten their livelihood viability. In many cases, the income from PES programmes does not reach the poor because of political and economic constraints. Local elite capture of PES benefits through the monopolization of access to forestland and existing state forestry management are identified as key problems. We argue that as PES schemes create a market for ecosystem services, such markets must be understood not simply as bald economic exchanges between ‘rational actors’ but rather as exchanges embedded in particular socio-political and historical contexts to support the sustainable use of forest resources and local livelihoods in Vietnam

Thymic Alterations in GM2 Gangliosidoses Model Mice

BACKGROUND: Sandhoff disease is a lysosomal storage disorder characterized by the absence of β-hexosaminidase and storage of GM2 ganglioside and related glycolipids. We have previously found that the progressive neurologic disease induced in Hexb(-/-) mice, an animal model for Sandhoff disease, is associated with the production of pathogenic anti-glycolipid autoantibodies. METHODOLOGY/PRINCIPAL FINDINGS: In our current study, we report on the alterations in the thymus during the development of mild to severe progressive neurologic disease. The thymus from Hexb(-/-) mice of greater than 15 weeks of age showed a marked decrease in the percentage of immature CD4(+)/CD8(+) T cells and a significantly increased number of CD4(+)/CD8(-) T cells. During involution, the levels of both apoptotic thymic cells and IgG deposits to T cells were found to have increased, whilst swollen macrophages were prominently observed, particularly in the cortex. We employed cDNA microarray analysis to monitor gene expression during the involution process and found that genes associated with the immune responses were upregulated, particularly those expressed in macrophages. CXCL13 was one of these upregulated genes and is expressed specifically in the thymus. B1 cells were also found to have increased in the thy mus. It is significant that these alterations in the thymus were reduced in FcRγ additionally disrupted Hexb(-/-) mice. CONCLUSIONS/SIGNIFICANCE: These results suggest that the FcRγ chain may render the usually poorly immunogenic thymus into an organ prone to autoimmune responses, including the chemotaxis of B1 cells toward CXCL13