The use of 99mTc as a tracer in the determination of 99Tc by ICPmass spectrometry

The aim of this paper is to carry out a deep study on the possibilities of using 99mTc from 99Mo-99mTc clinical isotope generators to trace the radiochemical separations for 99Tc measurements by ICP-MS. By appropriately de®ning a minimum detectable mass concentration (MDMC) for the mass spectrometer it is possible to conclude that such use is acceptable for a wide range of activities of the tracer. The MDMC increases from 0.41 to 0.51 ppt when the amount of 99mTc added to the samples ranges from 0 to 2 MBq. The purification of the final sample from Ru must be the main objective of the 99Tc measurement method, since its presence noticeably enhances the 99Tc MDMC

Functional explanation of extreme hatching asynchrony: Male Manipulation Hypothesis

Hatching asynchrony in birds is considered an adaptation to facilitate brood reduction because under conditions of food scarcity, the smallest nestling usually dies soon after hatching, thereby minimizing parental effort. However, in species with extreme hatching asynchrony, the last hatchlings paradoxically experience a very low probability of survival and death can take so long that it can hardly be considered an adaptation. Here, we propose and experimentally tested a new adaptive hypothesis explaining the brood reduction paradox, namely the “Male Manipulation Hypothesis”. Our hypothesis suggests that by inducing asynchronous hatching, females increase the feeding requirements of the brood, which will induce males to increase provisioning effort. In addition, females may extend the period of male manipulation by feeding the smallest nestling just enough to sustain life. Our study showed that male common blackbirds (Turdus merula) increased their effort (i.e., number of food items per hour) in experimental asynchronous broods compared to synchronous broods, while females reduced their contribution, as predicted by the hypothesis

Analog CMOS Readout Channel for Time and Amplitude Measurements With Radiation Sensitivity Analysis for Gain-Boosting Amplifiers

The front-end readout channel consists of a charge sensitive amplifier (CSA) and two different unipolar-shaping circuits to generate pulses suitable for time and energy measurement. The signal processing chain of the single channel is built of two different parallel processing paths: a fast path with a peaking time of 30 ns to obtain the time of arrival for each particle impinging the detector; and a slow path with a peaking time of 400 ns dedicated for low noise amplitude measurements, which is formed by a pole-zero cancellation circuit and a 4th order complex shaper based on a bridged-T architecture. The tunability of the system is accomplished by the discharge time constant of the CSA in order to accommodate various event rates. The readout system has been implemented in a 180 nm CMOS technology with the size of 525 μm x 290 μm . The building blocks use compact gain-boosting techniques based on quasi-floating gate (QFG) transistors achieving accurate energy measurement with good resolution. The high impedance nodes of QFG transistors require a detailed study of sensitivity to single-effect transients (SET). After carrying out this study, this paper proposes a method to select the value of the QFG capacitors, minimizing the area occupancy while maintaining robustness to radiation. The nonlinearity of the CSA-slow-shaper has been found to be less than 1% over a 10–70 fC input charge. The power dissipation of the readout channel is 4.1 mW with a supply voltage of 1.8 V.Ministerio de Ciencia, Innovación y Universidades PGC2018-095640-B-I00Consejería de Transformación Económica, Industria, Conocimiento y Universidades P18-FR-3852 y P18-FR-431

Real-Time Wireless Platform for In Vivo Monitoring of Bone Regeneration

For the monitoring of bone regeneration processes, the instrumentation of the fixation is an increasingly common technique to indirectly measure the evolution of bone formation instead of ex vivo measurements or traditional in vivo techniques, such as X-ray or visual review. A versatile instrumented external fixator capable of adapting to multiple bone regeneration processes was designed, as well as a wireless acquisition system for the data collection. The design and implementation of the overall architecture of such a system is described in this work, including the hardware, firmware, and mechanical components. The measurements are conditioned and subsequently sent to a PC via wireless communication to be in vivo displayed and analyzed using a developed real-time monitoring application. Moreover, a model for the in vivo estimation of the bone callus stiffness from collected data was defined. This model was validated in vitro using elastic springs, reporting promising results with respect to previous equipment, with average errors and uncertainties below 6.7% and 14.04%. The devices were also validated in vivo performing a bone lengthening treatment on a sheep metatarsus. The resulting system allowed the in vivo mechanical characterization of the bone callus during experimentation, providing a low-cost, simple, and highly reliable solution.Junta de Andalucía US-1261691Ministerio de Economía y Competitividad DPI2017-82501-PMinisterio de Economía y Competitividad PGC2018-097257-B-C3

Suppression of Coulomb-nuclear interference in the near-barrier elastic scattering of 17 Ne from 208 Pb

The proton drip-line nucleus 17Ne is considered a good candidate for a Borromean two-proton halo with a 15O + p+ pstructure. Angular distributions of the elastic scattering and inclusive 15O production for a 136 MeV 17Ne beam incident on a 208Pb target were measured for the first time at the SPIRAL1 facility, GANIL. Use of the GLORIA detector array allowed high-resolution data over a wide angular range from 20◦up to 95◦in the laboratory frame to be obtained. The elastic scattering angular distribution shows similarities with those for both 6He and 20Ne at equivalent collision energies with respect to the corresponding Coulomb barriers, exhibiting the suppression of the Coulomb rainbow peak characteristic of strong coupling. Optical model and coupled channel fits suggest that this is due to a combination of coupling to low-lying quadrupole resonances and Coulomb dipole coupling to the low-lying continuum, although their relative importance depends on the relevant B(E2)values which remain to be firmly determined

Circulating nitrate-nitrite reduces oxygen uptake for improving resistance exercise performance after rest time in well-trained CrossFit athletes

This study aimed to determine the effects of circulating nitrate plus nitrite (NOx) concentrations on resistance exercise performance, VO2 and biomarkers of muscle damage. Eleven well-trained male CrossFit athletes (29.2 ± 3.7 years, 78.9 ± 5.4 kg, 175.1 ± 6.3 cm) carried out a resistance exercise test after drinking 140 mL of beetroot juice (BJ) or placebo. The test consisted of repeating the same resistance exercise routine twice: wall ball shots plus full back squat with 3-min rest (1st routine) or without rest (2nd routine) between the two exercises. Higher NOx plasma levels were verified after BJ than placebo in the pretest and post-test (p < 0.001). A higher number of repetitions was observed after BJ intake compared to placebo in the full back squat exercise during the first routine (p = 0.004). A significantly reduced VO2 was detected after BJ intake compared to placebo during rest and full back squat execution in the first routine (p < 0.05). Plasma myoglobin concentrations were significantly increased with BJ compared to placebo (p = 0.036). These results showed that plasma NOx levels reduced VO2 after BJ intake during rest time. These reduced VO2 was a key factor for improving full back squat performance during the first routine

A smart sensor for defending against clock glitching attacks on the I2C protocol in robotic applications

This paper presents a study about hardware attacking and clock signal vulnerability. It considers a particular type of attack on the clock signal in the I2C protocol, and proposes the design of a new sensor for detecting and defending against this type of perturbation. The analysis of the attack and the defense is validated by means of a configurable experimental platform that emulates a differential drive robot. A set of experimental results confirm the interest of the studied vulnerabilities and the efficiency of the proposed sensor in defending against this type of situation

INTEGRACIÓN DE SISTEMA DE CONTROL DE MOVIMIENTO PARA MÁQUINA DE CORTE Y GRABADO CON LASER (INTEGRATION OF MOTION CONTROL SYSTEM FOR CUTTING AND ENGRAVING MACHINE WITH LASER)

Resumen Se presenta el desarrollo de una máquina de corte para aplicaciones textiles, la cual consta de tres elementos principales: sistema de posicionamiento a base de husillos, sistema de control electrónico y software de control. Se utilizó una tarjeta de propósito específico para el control de movimiento de National Instruments, que mediante una interfaz gráfica de usuario desarrollada en LabVIEW controla los motores de pasos de cada eje. Con un LASER de 15 W se logró realizar el corte y grabado sobre de tela. Se presentan también los resultados de precisión y exactitud de la máquina. Palabras Clave: Corte con LASER, Control de movimiento, Controladores de motores y Trayectoria. Abstract The development of a cutting machine for textile applications is presented, which consists of three main elements: ball screw-based positioning system, electronic control system and control software. A National Instruments motion control card was used, which, through a graphical user interface developed in LabVIEW, controls the stepper motors of each axis. With a LASER of 15 W it was possible to cut and engrave on fabric. The precision and accuracy results of the machine are also presented Keywords: LASER Cutting, Motion Control, Motor Controllers and Path

Controlling Robot Motion by Blinking Eyes: an Experience on Users Training

This article aims to describe a system designed to control the movement of mobile robots by blinking eyes. It is based on the use of a Brain Computer Interface and a particular control architecture. The paper addresses the key aspects that allow simplifying usersrobot interaction and proposes a control strategy that facilitates a fast learning of robot handling. In this sense, the main advantage of the approach is the short period of time required for users’ training. The article details a methodology aimed to evaluate this feature, presents experimental results that confirm this fact and also discusses about the influence of interacting with a real or a simulated robot. Particularly, it analyses if a previous training with the virtual robot helps to improve the interaction with the real robot or vice versa

Human Hereditary Cardiomyopathy Shares a Genetic Substrate With Bicuspid Aortic Valve.

The complex genetics underlying human cardiac disease is evidenced by its heterogenous manifestation, multigenic basis, and sporadic occurrence. These features have hampered disease modeling and mechanistic understanding. Here, we show that 2 structural cardiac diseases, left ventricular noncompaction (LVNC) and bicuspid aortic valve, can be caused by a set of inherited heterozygous gene mutations affecting the NOTCH ligand regulator MIB1 (MINDBOMB1) and cosegregating genes. We used CRISPR-Cas9 gene editing to generate mice harboring a nonsense or a missense MIB1 mutation that are both found in LVNC families. We also generated mice separately carrying these MIB1 mutations plus 5 additional cosegregating variants in the ASXL3, APCDD1, TMX3, CEP192, and BCL7A genes identified in these LVNC families by whole exome sequencing. Histological, developmental, and functional analyses of these mouse models were carried out by echocardiography and cardiac magnetic resonance imaging, together with gene expression profiling by RNA sequencing of both selected engineered mouse models and human induced pluripotent stem cell-derived cardiomyocytes. Potential biochemical interactions were assayed in vitro by coimmunoprecipitation and Western blot. Mice homozygous for the MIB1 nonsense mutation did not survive, and the mutation caused LVNC only in heteroallelic combination with a conditional allele inactivated in the myocardium. The heterozygous MIB1 missense allele leads to bicuspid aortic valve in a NOTCH-sensitized genetic background. These data suggest that development of LVNC is influenced by genetic modifiers present in affected families, whereas valve defects are highly sensitive to NOTCH haploinsufficiency. Whole exome sequencing of LVNC families revealed single-nucleotide gene variants of ASXL3, APCDD1, TMX3, CEP192, and BCL7A cosegregating with the MIB1 mutations and LVNC. In experiments with mice harboring the orthologous variants on the corresponding Mib1 backgrounds, triple heterozygous Mib1 Apcdd1 Asxl3 mice showed LVNC, whereas quadruple heterozygous Mib1 Cep192 Tmx3;Bcl7a mice developed bicuspid aortic valve and other valve-associated defects. Biochemical analysis suggested interactions between CEP192, BCL7A, and NOTCH. Gene expression profiling of mutant mouse hearts and human induced pluripotent stem cell-derived cardiomyocytes revealed increased cardiomyocyte proliferation and defective morphological and metabolic maturation. These findings reveal a shared genetic substrate underlying LVNC and bicuspid aortic valve in which MIB1-NOTCH variants plays a crucial role in heterozygous combination with cosegregating genetic modifiers.This study was supported by grants PID2019-104776RB-I00 and PID2020-120326RB-I00, CB16/11/00399 (CIBER CV) financed by MCIN/AEI/10.13039/501100011033, a grant from the Fundación BBVA (Ref. BIO14_298), and a grant from Fundació La Marató de TV3 (Ref. 20153431) to J.L.d.l.P. M.S.-A. was supported by a PhD contract from the Severo Ochoa Predoctor-al Program (SVP-2014-068723) of the MCIN/AEI/10.13039/501100011033. J.R.G.-B. was supported by SEC/FEC-INV-BAS 21/021. A.R. was funded by grants from MCIN (PID2021123925OB-I00), TerCel (RD16/0011/0024), AGAUR (2017-SGR-899), and Fundació La Marató de TV3 (201534-30). J.M.P.-P. was supported by RTI2018-095410-B-I00 (MCIN) and PY2000443 (Junta de Andalucía). B.I. was supported by the European Commission (H2020-HEALTH grant No. 945118) and by MCIN (PID2019-107332RB-I00). DO’R was sup-ported by the Medical Research Council (MC-A658-5QEB0) and KAMcG by the British Heart Foundation (RG/19/6/34387, RE/18/4/34215). The cost of this publication was supported in part with funds from the European Regional Devel-opment Fund. The Centro Nacional de Investigaciones Cardiovasculares is sup-ported by the ISCIII, the MCIN, and the Pro Centro Nacional de Investigaciones Cardiovasculares Foundation and is a Severo Ochoa Center of Excellence (grant CEX2020001041-S) financed by MCIN/AEI/10.13039/501100011033. For the purpose of open access, the authors have applied a CC BY public copyright license to any Author Accepted Manuscript version arising.S