Natural hybridization and introgression between the Long-Spurred orchid, <i>Orchis longicornu</i> Poiret and the Green-Winged orchid, <i>O. morio</i> L. (Orchidaceae) in Corsica

Genetic studies have revealed in Southern Corsica, near Bonifacio, not only the existence of sporadic F1 hybrids between Orchis longicornu Poiret and O. morio L., but of a hybrid zone where both parental taxa are more or less introgressed. Our data indicate complete interfertility between the two taxa, which should therefore be considered as subspecies of the same biological species

<i>Orchis longicornu</i> Poiret in Sardinia: genetic, morphological and chorological data

Data are given on the genetic structure, morphology and chorology of the Long-Spurred orchid, Orchis longicornu Poiret (1789) from Sardinia and the occurrence in this island of the morphologically similar Orchis morio L. (1753), often recorded for Sardinia, is investigated. The genetic analysis of 27 enzyme lo ci in population samples from locations where both species had been recorded showed that: (i) in the population samples from Sardinia the polymorphic loci are in Hardy-Weinberg equilibrium; (ii) these samples are genetically poorly differentiated from each other (average Nei's D = 0.01): less than 2% of the overall genetic variation observed is attributable to differences between populations (GST = 0.015); (iii) a high rate of gene flow was estimated between Sardinian populations: Nm ≈ 4, possibly owing to seed dispersal by wind; (iv) when Sardinian samples are compared with O. morio from continental Italy, significant differences in genetic variation were observed: average He = 0.16 in the former, 0.12 in thc latter; (v) the genetic distance found between Sardinian populations and those of O. morio from continental ltaly is relatively high: average Nei's D = 0.18, average Rogers' D = 0.22; (vi) highly significant differences in allele frequencies were found at a number of loci (Mdh-2, Sod-3, Pgm-1, Gpi-1, Gpi-2) between populations from Sardinia and continental Italy, each giving a probability of correct identification that varies from 0.90 to 1 (diagnostic loci). These findings provide evidence that O. morio is not present in the material genetically analyzed from Sardinia, which includes a single species, apparently corresponding to O. longicornu. The slight heterogeneity observed at some loci in the Sardinian population samples can be attributed in some cases (e.g. Mdh-1, Adh, Est-6) to local differences, possibly adaptive; in others (e.g. Pgm-1 and Gpi-l) to genetic drift effects. The hypothesis that some alleles (Gpi-1100, Dia107, NADHdh-296) found in O. morio and recorded at low frequency in a few Sardinian populations reflect palaeointrogressive phenomena (owing to sporadical immigrant individuals of O. morio having diluted their genes in O. longicornu genome through multiple generations of backcrosses) is not sufficiently supported by the available data. The genetic data are in agreement with the results from morphological studies. These involved the examination of about 1500 specimens (both alive and dried) from all over Sardinia, and their comparison with herbarium specimens of O. longicornu from patria typica (Algeria) and of O. morio from continental Italy. The typification of O. longicornu is given. The specimens from Sardinia correspond well, on a morphological basis, to the Algerian ones and therefore were all assigned to O. longicornu. Several characters were considered, differentiating O. longicornu from O. morio both in fresh and dried specimens. However, some of them, currently used in diagnostic keys, show much overlap between the two species. This explains the quotations of O. morio from Sardinia, that appear to be misidentifications of O. longicornu. A description of O. longicornu from Sardinia is provided, and its distribution in the island is defined, on the basis of (i) a critical revision of literature rccords, and (ii) the examination of fresh and dried Sardinian specimens. O. longicornu is shown to be widespread throughout Sardinia, living in many different habitats from 0 to 1500 m a.s.l

Neonatal screening for glucose-6-phosphate dehydrogenase deficiency fails to detect heterozygote females

We examined glucose-6-phosphate dehydrogenase (G6PD) deficiency in north-eastern Italian Caucasian neonates detected by neonatal screening, in order to measure the incidence of heterozygote females detected by neonatal screening, and to estimate the near-true total incidence. A total of 85,437 Caucasian neonates, born between January 2000 and December 2001, have been enclosed in the study. The total incidence of the disease, measured by fluorescent method, is 0.9‰; the total incidence, calculated by Hardy-Weinberg law, is 4.8‰. The frequency of missed females is 93% of total females expected with G6PD deficiency; most of them are very likely heterozygous females. The sensitivity of the fluorescent method might be not sufficient to detect all females. Since heterozygote females might develop the symptoms of G6PD deficiency later, these results suggest that the G6PD neonatal screening may not be helpful in preventing disease in female

A quantitative multivariate methodology for unsupervised class identification in pistachio (Pistacia vera L.) plant leaves size

Aim of study: Genetic diversity of pistachio, can be evaluated by using different descriptors, as adopted in international certification systems. Mainly the descriptors are morphological traits as leaf, which represents an important organ for its sensibility to growth conditions during the expansion phase. This study adopted a rapid and quantitative non-hierarchic clustering classification (k-means), to extract size classes basing on the contemporary combination of different morphological traits (i.e., leaf stalk length, terminal leaf length, terminal leaf width and terminal leaf ratio) of a varietal collection composed by 21 pistachio cultivars.Area of study: Worldwide.Material and methods: The unsupervised non-hierarchic clustering technique was adopted to the entire samples of pistachio leaves from k=2 to k=15 for both four morphological variables (i.e., leaf stalk length, terminal leaf length, terminal leaf width and terminal leaf ratio) and three morphological variables (i.e., terminal leaf length, terminal leaf width and terminal leaf ratio).Main results: A classification model only on the three morphological variables (for results of statistical analysis in which the groups resulted to be more separated and different for all the variables), with k= 5 (five groups), was constructed using a non-linear artificial neural network approach. The percentages of bad prediction in both training and testing resulted equal to 0%. The “terminal leaf length” returned the higher impact (44.89%).Research highlights: The contemporary combination of different morphological leaf traits, allowed to create an automatic classification of size classes of great importance for cultivar identification and comparison

The project \u201cInteractive Topography of Dante\u2019s Inferno\u201d : transfer of knowledge and design of didactic tools

The project \u201ctopography of Dante\u2019s inferno\u201d is an experiment on alternative mode of access to a complex text relying on an evident topographical structure. The artifact (a website) is designed with the aim of introducing young students (11\u201314 years old) to a text usually read and studied by older students (16\u201319 years old). The design of the artifact was based on the theories of Sinsem\ueda applied to interaction design, and the testing focused on (1) usability and (2) on the understanding of the topography of the poem as a precondition for understanding the text

Effects of physical activity on endothelial progenitor cells (EPCs)

Physical activity has a therapeutic role in cardiovascular disease (CVD), through its beneficial effects on endothelial function and cardiovascular system. Circulating endothelial progenitor cells (EPCs) are bone marrow (BM) derived cells that represent a novel therapeutic target in CVD patients, because of their ability to home to sites of ischemic injury and repair the damaged vessels. Several studies show that physical activity results in a significant increase in circulating EPCs, and, in particular, there are some evidence of the beneficial exercise-induced effects on EPCs activity in CVD settings, including coronary artery disease (CAD), heart failure (HF), and peripheral artery disease (PAD). The aim of this paper is to review the current evidence about the beneficial effects of physical exercise on endothelial function and EPCs levels and activity in both healthy subjects and patients with CVD

Multi-scale approach to analyse the evolution of soft rock coastal cliffs and role of controlling factors: a case study in South-Eastern Italy

The evolution of soft rock coastlines is strictly related to natural and anthropogenic conditions, which in some cases can determine also an acceleration of coastal retreat. The recent evolution of a soft rocky coastal stretch on the Italian southern Adriatic sector is analysed. To investigate the most important contributing factors to coastal evolution, a detailed multi-temporal (1954–2017) morphological analysis with photo-interpretation is carried out, which allows gaining a general understanding of the cliff evolution. The coastal retreat was then evaluated by exploiting the Digital Shoreline Analysis System (DSAS) tool. From that, site-specific analyses are carried out to explore the role of four environmental factors: rock mechanical properties, geostructural setup of the rock mass along the coast, emerged rock platform at the toe, and the storm waves. Analyses highlighted how the most significant retreat is detected along cliff segments characterized by the presence of low strength rocks susceptible to water-induced weakening, moderate fracturing degree of the rock mass, absence of the emerged platform at the toe, and wave storms. This work shows how a multi-scale methodology could represent an efficient approach to gain an interpretation of the instability processes and thus prepare risk mitigation plans and land management strategies

Genetic evidence of two sibling species within the Contracoecum ogmorhini Johnson & Mawson 1941 complex (Nematoda; Anisakidae) from otariid seals in boreal and austral regions

Genetic variation of Contracaecum ogmorhini (sensu lato) populations from different otariid seals of the northern and southern hemisphere was studied on the basis of 18 enzyme loci as well as preliminary sequence analysis of the mitochondrial cyt b gene (260 bp). Samples were collected from Zalophus californianus in the boreal region and from Arctocephalus pusillus pusillus, A. pusillus doriferus and A. australis from the austral region. Marked genetic heterogeneity was found between C. ogmorhini (sensu lato) samples from the boreal and austral region, respectively. Two loci (Mdh-2 and NADHdh) showed fixed differences and a further three loci (Iddh, Mdh-1 and 6Pgdh) were highly differentiated between boreal and austral samples. Their average genetic distance was DNei = 0.36 at isozyme level. At mitochondrial DNA level, an average proportion of nucleotide substitution of 3.7% was observed. These findings support the existence of two distinct sibling species, for which the names C. ogmorhini (sensu stricto) and C. margolisi n. sp., respectively, for the austral and boreal taxon, are proposed. A description for C. margolisi n. sp. is provided. No diagnostic morphological characters have so far been detected; on the other hand, two enzyme loci, Mdh-2 and NADHdh, fully diagnostic between the two species, can be used for the routine identification of males, females and larval stages. Mirounga leonina was found to host C. ogmorhini (s.s.) inmixed infections with C. osculatum (s.l.) (of which C. ogmorhini (s.l.) was in the past considered to be a synonym) and C. miroungae; no hybrid genotypes were found,confirming the reproductive isolation of these three anisakid species. The hosts and geographical range so far recorded for C. margolisi n. sp. and C. ogmorhini (s.s.) are given

Molecular targets and oxidative stress biomarkers in hepatocellular carcinoma: an overview

Hepatocellular carcinoma (HCC) is a complex and heterogeneous tumor with multiple genetic aberrations. Several molecular pathways involved in the regulation of proliferation and cell death are implicated in the hepatocarcinogenesis. The major etiological factors for HCC are both hepatitis B virus (HBV) and hepatitis C virus infection (HCV)

Superior EVOO Quality Production: An RGB Sorting Machine for Olive Classification

Extra virgin olive oil (EVOO) is a commercial product of high quality, thanks to its nutritional and organoleptic characteristics. The olives ripeness and the choice of harvest time according to their color and size, strongly influences the quality of the EVOO. The physical sorting of olives with machines performing rapid and objective optical selection, impossible by hand, can improve the quality of the final product. The aim of this study concerns the classification of olives into two qualitative classes, based on the maturity stage and the presence of external defects, through an industrial RGB optical sorting prototype, evaluating its performance and comparing the results with those obtained visually by trained operators. EVOOs obtained from classified olives were characterized through chemical, physical-chemical analysis and sensory profile. For the first time, the optoelectronic technologies in an industrial system was tested on olives to produce superior quality EVOO. The selection allows late harvest, obtaining oils with good characteristics from fully ripe and unripe fruits together, separating defective olives with appropriate calibration and training. Optoelectronic selection creates the opportunity to blend the obtained oils destined to different applications according to the needs of the consumer or producer, using a vanguard technology at low cost.11noAuthor Contributions Conceptualization, F.P., C.C. and S.V. (Simona Violino); methodology, F.P., S.V. (Simona Violino), F.T. and P.T.; software, S.V. (Simone Vasta), F.T. and C.C.; validation, F.P. and C.C.; formal analysis, S.V. (Simone Vasta), L.M., R.M., P.T., L.G., P.D.R. and L.O.; investigation, F.P. and C.C.; resources, L.M. and S.V. (Simona Violino); data curation, C.C, F.P, S.V. (Simona Violino), L.M., L.G. and P.D.R.; writing—original draft preparation, S.V. (Simona Violino) and L.M.; writing—review and editing, S.V. (Simone Vasta), L.M, L.G., C.C., P.D.R. and P.T.; visualization, F.P. and C.C.; supervision, F.P. and C.C.; project administration, F.P. and C.C.; funding acquisition, F.P. and C.C. All authors have read and agreed to the published version of the manuscript