Short-term isokinetic training increases muscle strength in young subjects

O objetivo foi avaliar os efeitos de treinamento isocinético de curta duração no desempenho muscular em indivíduos jovens. Onze homens sadios participaram de um programa de treinamento isocinético de curta duração, composto por 3 sessões (4 séries; 10 repetições isocinéticas concêntricas a 120º.s-1; 2 minutos de intervalo entre séries). A reprodutibilidade dos dados de 2 sessões foi avaliada pelo coeficiente de correlação intraclasses (CCI) e teste de Bland e Altman. As avaliações do treinamento foram aplicadas pré e pós a 2ª e 3ª sessões (1 série; 3 repetições concêntricas de extensão do joelho a 60º.s-1, 120º.s-1 e 180º.s-1). Utilizou-se a ANOVA para medidas repetidas e post-hoc de Tukey para verificar diferenças nos testes. O CCI variou de 0,97 a 0,98 em todas as velocidades. Ocorreu um aumento no pico de torque a 60º.s-1 (P=0,03) e 120º.s-1 (P=0,01) após 2 sessões de treinamento. Sugere-se que duas sessões de exercício isocinético foram suficientes para induzir ganhos de força na velocidade treinada (120º.s-1) e em velocidade de contração mais lenta (60º.s-1), em indivíduos jovens.The aim was to investigate the effects of short-term isokinetic training on muscle performance in young individuals. Eleven healthy males subjects underwent to short-term training, consisting of 3 sessions (4 sets, 10 repetitions of concentric isokinetic exercise at 120º.s-1; 2-minute interval between sets). Data reproducibility from two sessions was evaluated by intraclass correlation coefficient (ICC) and Bland and Altman test. Training assessments were made pre and post the 2nd and 3rd sessions (1 set, 3 repetitions of concentric knee extension at 60º.s-1, 120º.s-1 and 180º.s-1). An ANOVA for repeated measures and Tukey post-hoc test was applied to determine differences between tests. The ICC ranged from 0.97 to 0.98 for all velocities. There was an increase in peak torque at 60º.s-1 (P=0,03) and 120º.s-1 (P=0,01) after 2 training sessions. Two sessions of isokinetic exercise were sufficient to induce strength increases at the training velocity (120º.s-1) and the slower contraction velocity (60º.s-1) in young individuals

La relación entre la confianza y el compromiso y sus efectos en la lealtad de marca

This article considers the customer’s trust and commitment variables in attaining the much desired brand loyalty. In this sense, the present study proposed to validate a theoric model composed of three pillars - trust, commitment and brand loyalty, which would be useful for companies with a focus on brand equity. This study was one of the first in the Brazilian academic context to utilize the aforementioned pillars in analyzing a brand with more than three decades of history in the Brazilian music entertainment sector, this being the Rock in Rio Festival. By means of the Facebook Advertisements, 725 online questionnaires were responded by participants in the 2017 edition of the Rock in Rio. In addition, three hypotheses which initially were established became five 5 hypotheses during the statistical analysis process, 4 of which were supported. According to the empirical results obtained, the relationship between the trust/service expectation X commitment, trust/service expectation X loyalty, trust/service recovery X loyalty and commitment X loyalty constructs presented satisfactory results. Therefore, the use of the said constructs in the test model yielded for commitment an R2 of 57.7% and for loyalty an R2 of 83.1%, each of which respectively is positive. The findings of this work were established on content analysis and statistical procedures based on multivariate techniques.Este artículo toma en cuenta las variables confianza y compromiso del cliente para lograr la tan deseada lealtad hacia a una marca. En este sentido, se propone validar un modelo teórico compuesto por tres pilares, es decir, la confianza, el compromiso y la lealtad de marca que será útil para las empresas que poseen un enfoque orientado hacia el valor de marca (brand equity). Este estudio ha sido uno de los pioneros en el contexto académico de Brasil en usar dichos pilares al analizar una marca con más de tres décadas de historia en el sector del entretenimiento musical brasileño, que es la del Festival Rock in Rio. Se alcanzaron 725 cuestionarios en línea por medio del Facebook Ads que fueron aplicados a los participantes del Rock in Rio en la edición del año 2017. Además, se establecieron, inicialmente, 3 hipótesis que se convirtieron en 5 hipótesis durante el proceso de análisis estadístico, de las que 4 de ellas fueron aceptadas. Según los resultados empíricos obtenidos, las relaciones entre los constructos confianza/expectativa de los servicios frente a compromiso, confianza/expectativa de los servicios frente a lealtad, confianza/recuperación de los servicios frente a lealtad y compromiso frente a lealtad presentaron resultados satisfactorios. Así que la utilización de dichos constructos en el modelo de prueba produjo un R2 de un 57,7% para el compromiso y un 83,1% para la lealtad, que son respectivamente positivos. Los hallazgos de este trabajo han sido tratados con análisis de contenido y procedimientos estadísticos basados en técnicas multivariantes

GLUT1 expression in malignant tumors and its use as an immunodiagnostic marker

OBJECTIVE: To analyze glucose transporter 1 expression patterns in malignant tumors of various cell types and evaluate their diagnostic value by immunohistochemistry. INTRODUCTION: Glucose is the major source of energy for cells, and glucose transporter 1 is the most common glucose transporter in humans. Glucose transporter 1 is aberrantly expressed in several tumor types. Studies have implicated glucose transporter 1 expression as a prognostic and diagnostic marker in tumors, primarily in conjunction with positron emission tomography scan data. METHODS: Immunohistochemistry for glucose transporter 1 was performed in tissue microarray slides, comprising 1955 samples of malignant neoplasm from different cell types. RESULTS: Sarcomas, lymphomas, melanomas and hepatoblastomas did not express glucose transporter 1. Fortyseven per cent of prostate adenocarcinomas were positive, as were 29% of thyroid, 10% of gastric and 5% of breast adenocarcinomas. Thirty-six per cent of squamous cell carcinomas of the head and neck were positive, as were 42% of uterine cervix squamous cell carcinomas. Glioblastomas and retinoblastomas showed membranous glucose transporter 1 staining in 18.6% and 9.4% of all cases, respectively. Squamous cell carcinomas displayed membranous expression, whereas adenocarcinomas showed cytoplasmic glucose transporter 1 expression. CONCLUSION: Glucose transporter 1 showed variable expression in various tumor types. Its absence in sarcomas, melanomas, hepatoblastomas and lymphomas suggests that other glucose transporters mediate the glycolytic pathway in these tumors. The data suggest that glucose transporter 1 is a valuable immunohistochemical marker that can be used to identify patients for evaluation by positron emission tomography scan. The function of cytoplasmic glucose transporter 1 in adenocarcinomas must be further examined

The Parkinson's disease-associated GPR37 receptor interacts with striatal adenosine A2A receptor controlling its cell surface expression and function in vivo

G protein-coupled receptor 37 (GPR37) is an orphan receptor associated to Parkinson's disease (PD) neuropathology. Here, we identified GPR37 as an inhibitor of adenosine A2A receptor (A2AR) cell surface expression and function in vivo. In addition, we showed that GPR37 and A2AR do oligomerize in the striatum. Thus, a close proximity of GPR37 and A2AR at the postsynaptic level of striatal synapses was observed by double-labelling post-embedding immunogold detection. Indeed, the direct receptor-receptor interaction was further substantiated by proximity ligation in situ assay. Interestingly, GPR37 deletion promoted striatal A2AR cell surface expression that correlated well with an increased A2AR agonist-mediated cAMP accumulation, both in primary striatal neurons and nerve terminals. Furthermore, GPR37−/− mice showed enhanced A2AR agonist-induced catalepsy and an increased response to A2AR antagonist-mediated locomotor activity. Overall, these results revealed a key role for GPR37 controlling A2AR biology in the striatum, which may be relevant for PD management

LABAAN EM AÇÃO: PARTICIPAÇÃO NA CORRIDA DO SESI

Introdução: O laboratório de avaliação antropométrica (LABAAN) do Curso de Educação Física da UNOESC Campus Chapecó participou do evento Corrida do Bem promovido pelo SESI. Objetivo: O objetivo principal das avaliações era dar um feedback geral para cada avaliado, assim permitindo aconselhar-los sobre a pratica de exercício físico. Metodologia: Participaram 43 sujeitos com idade média 33,1±11,2. Foi utilizada a balança digital Bioland – EF934 (Florianópolis SC), o estadiômetro Cescorf (Porto Alegre, Brasil) e o Banco de Wells Sanny (São Paulo, Brasil) para avaliar massa corporal, estatura e flexibilidade. Para analisar a composição corporal foi utilizado IMC, e a flexibilidade através do teste de sentar e alcançar. Resultados: Os sujeitos apresentam estatura de 168±25,8 centimetros , massa corporal de 68,5±110,1 Kg, IMC de 24,8±1,7 Kg/m², flexibilidade de 27±10,1 cm, gordura de 16,9±5,6%  hidratação de 57,1±12,4%, massa muscular de 41,8±3,7% e massa óssea de 13,7±8,9Kg. Conclusão: Os participantes estão classificados com percentual de gordura e IMC saudável

Determinação simultânea de ácido ascórbico e ácido acetilsalicílico usando análise por injeção em fluxo com detecção amperométrica pulsada

A simple flow system with multiple pulse amperometric detection using a single working electrode is proposed for simultaneous determination of ascorbic (AA) and acetylsalicylic (AAS) acids in pharmaceutical formulations. The procedure is based on application of two potential pulses: 0.90 V/50 ms: oxidation and determination of AA without the interference of AAS; 1.35 V/50 ms: oxidation of both compounds and quantification of AAS by current subtraction using a correction factor. Sampling rate was estimated as 125 injections per hour and the limits of detection were 0.17 and 0.16 µmol L-1 for AA and AAS, respectively. Results for commercial samples agreed with those obtained using HPLC

PARTICIPAÇÃO DO CURSO DE EDUCAÇÃO FÍSICA UNOESC CAMPUS CHAPECÓ NA "CORRIDA DO BEM" DO SESI

A participação dos acadêmicos de Educação Física da Unoesc Campus de Chapecó em eventos esportivos ou acadêmicos é estimulado pelos professores do curso, já que esse tipo de participação agrega conhecimentos e possibilita a vivencia prática