NEW CONCEPT IN DENTOFACIAL ESTHETICS: THE BIOLOGICAL ASPECTS OF ADULT ORTHOPEDIC FACE MASK THERAPY: KONSEP BARU DALAM ESTETIKA DENTOFACIALDENTOFACIAL: ASPEK BIOLOGIS TERAPI TOPENG BEDAH TULANG DEWASA

Orthopedic face mask has been introduced for more than 100 years ago, however, it is primarily indicated for growingpatients. Its effectiveness in adult patients is still questionable. It is thought that in non growing adults the degree oforthopedic movement is minimal, whether dental movement i s more prominent. In addition, there is still controversial,whether patients’ compliance or the existent skeletal growth determines the treatment success. The objective of this studywas to propose a new concept for adult orthopedics face mask therapy ba sed on functional and biological mechanismsinvolved in dentofacial remodeling . Conventional face mask therapy only depended on duration, magnitude andunidirectional elastic forces. In the reviewed case report, the inspiration for a new concept was evolv ed from themultidirectional forces which were resulted from continuous functional movements (i.e. chewing and speaking) duringwearing face mask. These movements may lead to stimulate additional orthopedic or skeletal movements in adult patient.The expla nation of this phenomenon could be explained not only by clinical result, but also with the biologicalmechanism of bone remodeling. In conclusion, regarding to the successful treatment result and its logical biologicalexplanation, this new concept to inc rease the effectiveness of adult orthopedic face mask therapy is likely

Stimulation of osteoblast activity by induction of Aloe vera and xenograft combination

Background: Tooth extraction is generally followed by alveolar ridge resorption that later can cause flat ridge. Aloe vera have biogenic stimulator and hormone activities for wound healing. Purpose: This study was aimed to know osteoblast activities in alveolar bone after induction of Aloe vera and XCB combination. Methods: Fifty four of Cavia cabaya were divided into three main groups. Group I was control group. Group II was filled with xenograft concelous bovine (XCB) and group III was filled with the combination of Aloe vera gel and XCB. Then, each group was divided into three sub groups according to timing, they are 14, 30, and 60 days after tooth extraction and application. Histology and morphology examination were performed on the harvested specimens. Results: There were significant differences between the control group and the other groups filled with the combination of Aloe vera and XCB. Conclusion: In conclusion, the application of Aloe vera gel and xenograft combination decrease the number of osteoclast and increase the number of osteoblast in post tooth extraction alveolar bone structure indicating the new growth of alveolar bone

Cytotoxicity of Combination Chitosan with Different Molecular Weight and Ethanol Extracted Aloe vera using MTT Assay

The use of combination chitosan and ethanol extracted Aloe vera is considered to have activity to promote bone healing. The requirement dental material used in oral cavity include good biocompatibility, non toxic, non carcinogenic and nor allergenic response. The aim of this study was to examine the cytotoxicity combination chitosan with different molecular weight and ethanol extracted Aloe vera using MTT assay. 1 % chitosan gel (w/v) was made from chitosan powder with high (ChH) and low (ChL) molecular weight. 1 % chitosan gel was combined with ethanol extracted Aloe vera with concentration 50 % (Av1) and 75% (Av2). It divided five groups: control group, ChH-Av1, ChH-Av2, ChL-Av1 and ChL-Av2. Each of sample was immersed in eppendorf microtubes consist of media culture. After 24 hours, the immersion of media culture were used to investigate the cytotoxic effect to BHK-21 cell lines by MTT assay method. The density of optic formazan was indicated the number of living cells. All data were statistically analyzed by one-way Anova. The result showed that percentage of the living cells of all groups was uper 50 % using parameter CD50. There was no significant difference among treatment groups. It can be concluded that combination chitosan with different molecular weight and ethanol extracted Aloe vera were non toxic for BHK-21 cell culture

SAP3 Gene Expression as Diagnostic Marker of Oral Candidiasis In HIV/AIDS Patients

HIV/AIDS cases growth progressively. Oral Candidiasis frequently found in the tract of pathogenesis HIV infection, these condition due to immunocompromised condition among HIV/AIDS patient and one higher complications insidence in oral. Until now, type of cell and receptor of oral mucosa as particularly target of HIV has not been well understood. Secretory Aspartyl Proteinase (SAP) is hydrolytic enzyme secreted by C. albicans which might destruct protein in the site of infection. This enzyme coding by SAP gene and contribute to C. albicans virulency. The aim of this research is to found the expression of SAP3 as the predictor of HIV severity. 30 HIV patients divided into two groups, one group with ARV therapeutic indication (15 patients) and the other group without ARV therapeutic indication. The cotton swab has been done in buccal mucous and dorsum of tongue. The detection would be done by PCR. The result is showed in non-ARV, revealed the presence of SAP3 gene in all samples, but SAP3 gene was not found in all HIV/AIDS samples with ARV. SAP3 could be used as indicator of HIV/AIDS severity, since it was an oral infecton marker related with the degree of host immunodeficiency and C. albicans virulence level

THE INFLUENCE OF Aloe vera AND XENOGRAFT (XCB) TOWARD OF BONE MORPHO PROTEIN 2 ( BMP2) EXPRESSION AND AMOUNT OF OSTEOBLAST OF ALVEOLAR BONE INDUCED INTO TOOTH EXTRACTION SOCKETS (Cavia cobaya)

Tooth extraction can cause inflammation leading to alveolar ridge resorption. in addition, prominent ridge has crucial role for making denture successfully. Thus, socket preservation is needed to prevent greater aloveolar ridge redorption. an innovative material, a combination of aloe vera and xenograft (XCB

PROFIL PASIEN DAN PROFIL PENGOBATAN DIARE DI KLINIK MEDIS UBAYA

Diarrhea is the increased frequency of bowel movement, with abnormal delivery of liquid or soft stools. Drug Related Problems are events or circumstances experienced but not desired by patients who are involved or suspected of involving in drug therapies that are actual and potential which can affect their health conditions. The purpose of this study was to analyze the profile treatment of diarrhea in patients at the Klinik Medis Universitas Surabaya. Research conducted on the data of diarrhea cases in July-December 2008. As the sample in this study we used 48 people which were the total of the population. Based on demographic study, profile of patients with diarrhea, the most widely sexes suffer from diarrhea were female (64.58%). Age of the patients were mostly around 21 years old (29.17%). Class of drugs that were most widely used by people with diarrhea was antimotilitas (87.5%). The most widely drug used was loperamide hydrochloride (87.5%). There were no Drug Related Problems associated with treatment of diarrhea in patients at the Klinik Medis Universitas Surabaya

NEW CONCEPT IN DENTOFACIAL ESTHETICS: THE BIOLOGICAL ASPECTS OF ADULT ORTHOPEDIC FACE MASK THERAPY

Orthopedic face mask has been introduced for more than 100 years ago, however, it is primarily indicated for growing patients. Its effectiveness in adult patients is still questionable. It is thought that in non-growing adults the degree of orthopedic movement is minimal, whether dental movement is more prominent. In addition, there is still controversial, whether patients’ compliance or the existent skeletal growth determines the treatment success. The objective of this study was to propose a new concept for adult orthopedics face mask therapy based on functional and biological mechanisms involved in dentofacial remodeling. Conventional face mask therapy only depended on duration, magnitude and unidirectional elastic forces. In the reviewed case report, the inspiration for a new concept was evolved from the multidirectional forces which were resulted from continuous functional movements (i.e. chewing and speaking) during wearing face mask. These movements may lead to stimulate additional orthopedic or skeletal movements in adult patient. The explanation of this phenomenon could be explained not only by clinical result, but also with the biological mechanism of bone remodeling. In conclusion, regarding to the successful treatment result and its logical biological explanation, this new concept to increase the effectiveness of adult orthopedic face mask therapy is likely

Socioeconomic characteristics of the parents and the risk prediction of early childhood caries

Background: The high prevalence of early childhood caries still becomes a major health problem. It is because children prefer to consume sweet foods, which are also cariogenic. The oral mucosa is a mirror of general health or any systemic disease which usually shows visible symptoms in the oral cavity. Dental caries in children is often related to the socioeconomic characteristics of the parents. Some references suggest that there is a relationship between the socioeconomic status with the incidence of caries. Purpose: The purpose of this study was to examine the relationship of socioeconomic characteristics of the parents with the risk prediction of early childhood caries by using cariogram approach to the elementary school students in Wonosobo. Method: The research is an observational analytic study with cross-sectional approach survey method. The research sampling uses proportional random sampling with 201 respondents of fifth graders. The socioeconomic status is measured through questionnaires, while the risk prediction of early childhood caries is measured by using cariogram. The research data analysis uses Spearman rank. Result: The results of the study show that the socioeconomic characteristics of the respondents’ parents mostly belong in the middle category as many as 145 respondents (72.1%), lower category as many as 31 respondents (15.4%) and in the upper category as many as 25 respondents (12.4%). Prediction of the respondents avoiding early childhood caries is 55%, while the prediction of the respondents being risked of having early childhood caries is 45%. The results of data analysis show that the relationship between the socioeconomic characteristics of the respondents’ parents and the risk of having early childhood caries for the respondents is p<0.05. Conclusion: There is a relationship between the socioeconomic characteristics of the respondents’ parents with the early childhood caries, which shows that the higher the socioeconomic status of the parents, the lower the risk of the respondents from having early childhood caries

ANALYSIS OF IFN-yCONCENTRATION INWISTAR RAT BLOOD AFTER ORAL ADMINISTRATIONOF STANDARDIZED GREEN TEA WATER EXTRACT

ABSTRACT Green tea and its polyphenols have been studied extensively as cancer chemopreventive agents in recent years. However, the mechanisms of action are still not clearly understood. Some researchers suggest that immune system plays important role to destroy cancer cells. Because of that reason, the present study was designed to analyse the effects of oral administration standardized green tea water extract on increasing of IFN-y blood concentration and to elucidate possible mechanismsinvolved in the inhibitory action of the cancer development. Two groups (male and female) of 5 rats have given p.o. administration 1.25%of standardizedgreen tea water extract and got 300 mg of (-)-epigal/ocatechingal/ate (EGCG)/kg body weight, while two groups others (male and female) were used as control. We found that IFN-y blood concentration on male and female Wistar rat are significantly increase with 13.11% and 17.59%, respectively (p::,0.05)by ELISA method, whereas control had no increase. These results indicate that standardized green tea water extract has possibility to prevent and suppress cancer development through enhancing IFN-r expressionand other immunosurveil/ ancecomponent. Keywords: Green tea water extract, IFN-y, ELIS

AKTIVITAS EXPRESSI KOLAGEN II DAN OSTEOCALSIN TULANG ALVEOL AKIBAT PRESERVASI SOKET PENCABUTAN GIGI DENGAN CAMPURAN ALOE VERA DAN GRAFT 0,5%: EXPRESSION ACTIVITY OF COLLAGEN II AND OSTEOCALCYN ALVEOLAR BONE QONSEQUENT PRESERVATION POST EXTRACTION SOCKET FILLED WITH A MIXTURE OF ALOE VERA AND GRAFT 0,5%

Aloe vera adalah tanaman yang mempunyai fungsi antibiotik, anti inflamasi, anti jamur dan bahan yang merupakanbiogenik stimulator untuk merangsang bovine graft agar mempercepat pertumbuhan tulang. Tujuan penelitian ini adalahuntuk mengetahui mekanisme aktivitas osteoblas pada proses pembentukan tulang alveol dengan pemberian kombinasiAloe vera dan XCB. Metode penelitian adalah dengan melakukan uji laboratoris dengan hewan coba: 54 ekorcaviacabaya, dibagi menjadi 9 kelompok, masing-masing 6 ekor, terdiri atas 3 group, yang pertama kelompok kontrolyaitu hanya dicabut saja tanpa perlakuan, kelompok 2 adalah kelompok yang setelah gigi dicabut diberi XCB saja dankelompok ke 3 adalah kelompok yang setelah pencabutan diberi campuran Aloe vera dengan XCB pada soket bekaspencabutan gigi. Pemeriksaan dilakukan setelah 14, 30 dan 60 hari. Kemudian diperiksa ekspresi osteocalcin dan kolagentipe II sebagai marker terjadinya tulang alveol dengan pemeriksaan imunohistokimia. Hasil penelitian menunjukkanterdapat perbedaan bermakna ekpresi osteocalcin dan collagen II antara kelompok kontrol dengan kelompok yang diberiAloe vera + XCB pada, 14, 30 dan 60 hari setelah pencabutan. Sebagai kesimpulan, penelitian aktivitas pertumbuhanosteoblas pada tulang alveol ditandai dengan peningkatan ekpresi osteocalcin dan kolagen II