Analisis Pertumbuhan Kedelai Di Lahan Kering Dengan Aplikasi Bradyrhizobium Japonicum Yang Diinduksi Genistein Dan Pupuk Organik

This study aimed to get the information of soybean growth response on inoculation of B. japonicum induced genistein under dry land. The experiment was conducted in farmer fields at Desa Sambirejo, Langkat on June-September 2012. The study used a factorial randomized block design with two factors and three replications. The first factor is genistein induction treatment consisting of without and genistein (50 μM). The second factor is the treatment of B. japonicum and organic fertilizer consisted of without B. japonicum; B. japonicum; B. japonicum + manure 10 t/ha; B. japonicum straw compost + 10 t/ha; B. japonicum + manure 5 t/ha + straw compost 5 t /ha. The variables observed are plant growth rate, plant growth relative and nett assimilation rate. The results showed that treatment of genistein (50 μM) decreased the plant growth rate 5-6 WAP, plant growth relative 5-6 WAP and nett assimilation rate . Treatment of B. japonicum + manure 5 t/ ha + straw compost 5 t/ ha tent to increase plant growth rate, plant growth relative and nett assimilation rate. The interaction between genistein induction and treatment B. japonicum + organic fertilizer tended to decrease plant growth rate, plant growth relative and nett assimilation rate

Genetics Diversity Analysis of Progeny F3 Soybean (Glycine max L.) Tolerant Salinity Using Microsatellite Markers

Salt stress is one of the abiotic stresses that significantly reduce the yield of soybean. One of the strategies of plant breeding to produce soybean varieties that are resistant salinity is using artificial crosses that aimed to combine the properties of both parent genetics. SSR molecular markers can help to identify the genetic distribution that brings the character of salinity resistant high yielding of soybean. The purpose of this study were to determine the diversity of the progeny of soybean saline-tolerant and high yielding, information level of polymorphism using four SSR markers, determined the distance genotype progeny soybean with the elders and information on the available genetic diversity for selecting progeny candidates for possible use in a soybean breeding program of saline-tolerant soybean. Studies of genetic distance of 60 progeny soybean and 8 female individual numbers (61-64 ) and male (65-68) used four primary SSR markers was conducted in the Laboratory of Biomolecular Sucofindo Seed Production. This research used seeds were planted in crops with salinity levels of 5-6 dS / m-1 in a glass house at the Faculty of Agriculture, University of Sumatera Utara. Methods of SSR markers was applied with four SSR primers, 60 progeny soybean hybridization and eight numbers of female and male elders. The results showed the fourth primer is polymorphism with PIC average of 0:58. Cluster analysis divides the two groups. Only individuals who were outside 52 male group of male elders. There were 59 females and the progeny soybean traits such as female parent and male. F3 progeny soybean derivative Anjasmoro hybridization with saline resistant genotypes potential for genetic improvement to produce high saline resistant crops in order to support the expansion planting to support the improvement of soybean production in Indonesia.Keywords: soybean, progeny soybean hybridization,saline-tolerant, microsatellite marker

Effect of indigenous mycorrhizal fungi on organic osmotic adjusment in soybean under salt stress

Research about influence of indigenous mycorrhizal fungi on organic osmotic adjustment to salinity stress in soybean has been implemented in saline land in Percut Sei Tuan sub district, Regency of Deli Serdang with EC 4-5 mmhos/cm. This study aimed to determine the effect of indigenous mycorrhizalinoculation on organic osmotic adjusment in different soybean varities. This study used Split Plot Design with two plots. The main plot was soybean varieties consist of non-selected Grobogan variety (salt-sensitive variety) and F5 selected Grobogan variety (salt-tolerant variety). Split plot was mycorrhizal inoculumconsists of 7 types of mycorrhizal inoculum namely without inoculum, Glomus sp-1, Glomus sp-2, Glomus sp-3, Glomus sp-4, Glomus sp-5, and combined of all mycorrhizal inoculum. The parameters observed were leaf proline content, reducing sugar and soluble protein. The results showed that leaf proline content,reducing sugar content and soluble protein were significantly different in soybean varieties, mycorrhizal significantly affected leaf proline content, reducing sugar and soluble protein, while the soybean varieties and mycorrhizal interaction significantly affected soluble protein content in leaves

Growth And Production Of Several Rice Varieties With The Biochar From Different Sources Of Materials

This research will be carried out in the experimental land of the Tanjung Morawa Seed Center, Lubuk Pakam District, Deli Serdang Regency. The purpose of this research is to find out how the growth and production of several varieties of irrigated rice with biochar from different source. The experimental design used in this study was a split plot design with 2 factors. The first factor is: The provision of various sources of biochar with 5 levels of treatment: No biochar, rice husk biochar, coconut shell biochar, oil palm empty bunch biochar and jengkol skin biochar. The second factor was: Ciherang, Hipa 19, Inpari 33. Parameters observed were plant height (cm), number of panicles, weight of 1000 grains (g  and soil analysis. The results showed that the use of several varieties of lowland rice on the growth and production of irrigated rice had a significant effect on the height of rice plants from 6-9 WAP. The use of several varieties of  rice such as Ciherang, Hipa 10 and Inpari 33 varieties showed differences in plant height starting at the age of 6 WAP. The results of the final analysis of paddy fields after being given biochar treatment experienced an increase in nutrients such as N-total, P, K and organic C in all treatments

DETEKSI ANTIBIOTIKA PADA ISOLAT STREPTOMYCES YANG DIISOLASI DARI KOMPOS SAMPAH ORGANIK

PENELITIAN INI BERTUJUAN UNTUK MENGETAHUI POTENSI STREPTOMYCES YANG DIISOLASI DARI KOMPOS SAMPAH ORGANIK UDPK BRATANG DAN PENGOLAHAN LIMBAH RPH KRIAN SEBAGAI PENGHASIL BIOTIKA

Produksi dan Fisiologi Kedelai pada Kondisi Cekaman Kekeringan dengan Aplikasi Bradyrhizobium japonicum yang Diberi Penginduksi Genistein

ABSTRACTMutualism symbiosis between B. japonicum and soybean root is a complex case and managed by molecular signal exchange. Genistein is one of the main isoflavones in soybean root exudates which is responsive to nod gen induction in B. japonicum. Drought stress could give negative effect on the symbiosis. The aim of the research was to study the role of B. japonicum induced by genistein on production and physiological characters of soybean under drought stress. The experiment was conducted in a green house, Faculty of Agriculture, University of Sumatera Utara, using a randomized complete block design with three factors. The first factor was genistein induction, consisted of  with and without genistein. The second factor  was B. japonicum inoculation consisted of  without B. japonicum,  isolate 1, isolate 2 and isolate 3. The third factor  was soil water content condition, consisted of 40%, 60% and 80% of field capacity. The parameters observed were shoot dry weight, root dry weight,  net assimilation rate, dry weight per plant, 50 seeds weight and proline content. The result showed that increasing drought stress conditions decreased shoot dry weight, root dry weight, net assimilation rate, effective nodules number, seeds dry weight per plant and 50 seeds weight. The result showed that as an inducer of B. japonicum in drought stress condition, genistein reduced the effects of water stress on root nodulation of soybean. Proline acts as osmoregulator for increasing tolerance to drought stress in plants.Keywords: drought, genistein, soybean, root nodulatio

Produksi dan Fisiologi Kedelai pada Kondisi Cekaman Kekeringan dengan Aplikasi Bradyrhizobium japonicum yang Diberi Penginduksi Genistein

ABSTRACTMutualism symbiosis between B. japonicum and soybean root is a complex case and managed by molecular signal exchange. Genistein is one of the main isoflavones in soybean root exudates which is responsive to nod gen induction in B. japonicum. Drought stress could give negative effect on the symbiosis. The aim of the research was to study the role of B. japonicum induced by genistein on production and physiological characters of soybean under drought stress. The experiment was conducted in a green house, Faculty of Agriculture, University of Sumatera Utara, using a randomized complete block design with three factors. The first factor was genistein induction, consisted of  with and without genistein. The second factor  was B. japonicum inoculation consisted of  without B. japonicum,  isolate 1, isolate 2 and isolate 3. The third factor  was soil water content condition, consisted of 40%, 60% and 80% of field capacity. The parameters observed were shoot dry weight, root dry weight,  net assimilation rate, dry weight per plant, 50 seeds weight and proline content. The result showed that increasing drought stress conditions decreased shoot dry weight, root dry weight, net assimilation rate, effective nodules number, seeds dry weight per plant and 50 seeds weight. The result showed that as an inducer of B. japonicum in drought stress condition, genistein reduced the effects of water stress on root nodulation of soybean. Proline acts as osmoregulator for increasing tolerance to drought stress in plants.Keywords: drought, genistein, soybean, root nodulatio

UPAYA PENINGKATAN KEMANDIRIAN DAN KEWIRAUSAHAAN SISWA MELALUI PROGRAM SEKOLAH BERKEBUN

OSIS and Production Unit SMKS IT Marinah Al-Hidayah Medan are two elements that work together in the development of SMKS IT Marinah Al-Hidayah Medan. Production Unit which is media for students to develop creativity, interests and talents as well as place students to add experience and income with entrepreneurship. The activities to be carried out are the cultivation of oyster mushrooms, the utilization of mushrooms baglog waste and agricultural waste as compost raw material for use as a medium in vertical agricultural systems. However, the problem faced is the difficulty of supplying good quality oyster mushroom independently and also limited information about the technical culture of oyster mushroom cultivation, baglog waste handling and agricultural waste and utilization of school land for vegetable cultivation by vertikultur method. To help overcome these problems, the community service team of the Faculty of Agriculture USU through the Garden School Program conducts training on producing good quality oyster mushroom seeds by introducing mushroom seed making tools, press baglog and 20 m2 of growing room. Wastes baglog mushrooms and agricultural waste from around the school environment then processed into compost using compost machine. The resulting compost is used as a medium for planting organic vegetables using vertikulture cultivation system. Students are also trained to process a variety of food products made from oyster mushroom so that the shelf life, nutritional content and economic value of oyster mushrooms can be increased so it can be marketed to train to increase self-reliance with entrepreneurship

Respons Pertumbuhan Dan Produksi Kedelai Hasil Seleksi Terhadap Pemberian Asam Askorbat Dan Inokulasi Fungi Mikoriza Arbuskular Di Tanah Salin

Using of saline land to grow up soybean has constraints such yield decreasing. By using ofsaline tolerant soybean, using ascorbate acid, and inoculation of mychorrizha vesicular arbuscular(MVA) aimed to increasing growth and yield of soybean in saline land.This research was conductedat Kecamatan Percut Sei Tuan Kabupaten Deli Serdang in Februari – Mei 2013, using split split plotdesign with three factors, i.e. variety of saline tolerant soybean (Grobogan no selection andGrobogan F4 selection), giving of ascorbate acid (0 dan 500 ppm) and MVA isolate (MVA type 1,type 2, type 3, type 4 and type 5). Parameter observed were plant height, number of leaves, numberof productive branches, production per plant.The result of the research showed that using of salinetolerant soybean significantly increase all parameters observed. Giving of ascorbate acidsignificantly increase number of productive branches. Inoculation of MVA significantly effect allparameters observed. Interaction between variety of saline tolerant soybean and giving ascorbateacid were not significantly effect all parameters observed. Interaction between variety of salinetolerant soybean and giving of MVA significantly effected number of leaves 5 week after planting(WAP) and production per plant. Interaction between giving of ascorbate acid and MVA were notsignificantly effect all parameters observed. Interaction of three factors were not significantly effectall parameters observed