Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter
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Rejuvenation and Characterization of Local Rice Germplasm (Orya sativa L.) Under Organic Cultivation Syste
The research was carried out to rejuvenate and evaluate the performances of plant characters of rice genotyps to produce a pure lines that are suitable for organic cultivation method. Thirty genotypes of the selected local rice were conducted to evaluate the phenotype performances under the organic cultivation method. The rice genotypes were utilized in this experiment to evaluate their morpho-agronomic characters under organic cultivation system. Characterization of seed performances of each accession was described and catalogued to identify their accession traits. In addition, genotypic differences and similarities were also characterized and catalogued. Duplicate accessions were eliminated from the same variety or nearly identical variants of a variety. The morpho-agronomic performances of plant height, number of tillers, lifespan, number of panicles, filled grains per spike, 1000 grains weight, grain yield potential, and harvest index were clearly affected by the genotype. The results showed that the genotypes were greatly varied the morphoagronomic performances. , if organic system was applied, it was found that the responses of rice growth and yield of local varieties of Aceh is better than rice varieties cultivated by the national and introduction genotype
Exploration of Pulp and Husk of Gayo Arabica Coffee as Raw Material of Pectin – SWOT, Risk and Chemical Component Analysis
Advances in waste management offer huge possibilities for economic and social utilization of residues from coffee production such as the pulp and husk. Coffee pulp are obtained during coffee production around 20-45% of raw material either it processed wet or dry. In Gayo Highland areas, as a centre of Arabica production in Indonesia, coffee production known as semi-drying process where numbers of coffee residues arise up to 40% of total coffee berries. For local farmers pulp and husks often be decompose by burning, whether several alternatives have been attempted individually, either used as fertilizer, livestock feed and compost. However the applications above cover only small number of robust coffee residues. Therefore studies have been conducted to explore possibility to extract pectin from coffee pulp. Implementation of SWOT analysis and Risk Analysis by FMEA gain to enhance the information of strength, weakness, opportunity and threats of extraction pectin from coffee pulp/husks demonstrated that this attempt have higher possibility to be more efficient than others trial for both economically and ecologically. Furthermore, moisture contents, ash and crude fiber are examined from fresh pulp/husk which are stored in room temperatures for 16 days, 24 days and 30 days. The data demonstrated that length of storage up to 30 days has impact on increasing ash (up to 12.66% from 1.38%) and solid fiber contents (up to 28.24% from 2.70%) whilst moisture are decreased (down to 15.51% from 86.08%) which emphasizes that delaying production or prolonging the storage of coffee residues might not have any impact on product qualit
Morphology and Parasitaemia Development of Plasmodium berghei in Balb/c Mice (Mus musculus)
Malaria is one of the most severe public health problems worldwide. It is a leading cause of death and disease in many developing countries, where young children and pregnant women are the groups most affected. Malaria disease caused by Plasmodium parasite have symptoms that typically include fever, fatigue, vomiting and headaches. In severe cases, it can cause yellow skin, seizures, coma or death. The present study is aimed to monitoring parasitemia level and percentage of parasite morphology as parasitaemia progresses. This research used Plasmodium berghei NK strain obtained from National University of Malaysia which originally from MR4, USA. Design used in this research was completely randomized design, with 2 treatments which were mice without infection and mice with infection of P. berghei parasite. Method used in this research was staining method of thin smear of blood using Giemsa stained and observed by microscopic. Parameter of observation were morphology and development of P. berghei at different level of parasitaemia (10%, 20%, 30% and 40%). The result showed from microscopic examination of blood slides prepared from the study animals indicated that the ring form was dominant stage obtained at all different stages of infection followed by trophozoite stage. Meanwhile schizont stage was the lowest stage obtained at all different stages observed
Pharmacokinetics Interaction of Glucocorticoids with 99mTc-MDP Radiopharmaceuticals for Bone Imaging Agents and its Biodistribution Pattern
A drug therapy can alter the pharmacokinetic profiles and biodistribution patterns of radiopharmaceuticals. Glucocorticoids are pharmaceutical drugs for anti-inflammatory by preventing phospholipid release and decreasing eosinophil action. To achieve an optimum diagnostic outcome, this research was focused on pharmacokinetics interaction and biodistribution pattern between two kinds of Glucocorticoids drugs i.e. dexamethasone and prednisone with 99mTc- MDP using animal model Mus musculus stock Swiss. 99mTc-MDP has been developed as radiopharmaceutical for bone imaging in nuclear medicine. Mice were divided into three groups, which were treated with dexamethasone by oral administration for 5 days continously, treatment with prednisone by oral administration for 3 days continously and without treatment (control). Pharmacokinetics interaction was conducted by injecting 200mL 99mTc-MDP intravenously administrated using a dose 1 mCi/mL. Biodistribution pattern was conducted by injecting 200 mL99mTc-MDP intravenously administrated using a dose 1 mCi/mL. After 3 hours after intravenousinjection of 99mTc-MDP each of these groups of animals were killed with chloroform and then dissected. Radioactivity of blood samples and selected organs were weighed and counted by using single channel analyzer. The results of pharmacokinetics study showed that the elimination half-life of animal model that given with dexamethasone and prednisone are 4.61 h and 4.63 h more faster than control animals (20.67 h). The results of biodistribution study showed that uptake of 99mTc- MDP in bone using animal models decreased which were given dexamethasone and prednisone compared to normal animals, which following results 3.53 ± 0.49%, 3.47 ± 0.5% and 11.54 ±4.36% (control)
Physiological Seed Performance of Local Aceh and National Release Variety of Rice (Oryza sativa L.) to Water Stress
Global warming causes changes in rainfall patterns causing lack of water for cultivation (IPPC,2007). About 50% of the rice cultivation area in the world has water shortages which becomes limiting factors of production (Bouman, 2009), hence the need early detection for drought- resistant varieties with seed physiological test. The experiment carry out at Laboratory Seed Technology and Industry of Agriculture Faculty Syiah Kuala University from April to June 2015. The experiment in split plot design 3X10 with 3 replication and standing roll wet paper germination method used. Amount of 2250 seed are used for physiological seed performance test. Water stress condition induced with PEG 6000 0%; 12.5%; 25%. Local genotype and national released variety of rice used in this experiment are Bo Santeut, Romokot, Sanbei, Pade mas, Aweuh, Inpari 16, IR 64, Situ Patenggang, and Towuti. Concentrations of Polyethilene Glicol 6000 (PEG 6000) have significant effect on all physiological parameter. Some local rice Aceh genotypes potentially to be cultivated on dry lands to cope water stress conditions
Colonization Ability of Biological Control Agent Tricoderma spp on Cocoa Pod and Seedling
Some of Trichoderma species as antagonistic fungi are usually considered soil microorganism, They colonize plant roots, some- times forming a symbiotic relationship. Three species of Trichoderma (T. virens, T. harzianum and T. asperellum) have been inoculated on cacao seedling and cocoa pod. Trichoderma species can be re-isolated from surface sterilized cacao seedling, including the stem and leaf, root, and pod then observed their colonization ability. Fungal hyphae were observed under the microscope emerging from the leaf, steam, root of seedling and pod as soon as 1 day after their isolation from surface sterilized cacao seedling and pod. All Trichoderma species were able to enter and make colonization. The highest percentage of colonization occurred in the T. harzianum by 73.3% (leaves), 46.7% (trunk) and 86.7% (roots). While colonization on the skin cocoa pod (epidermis) also has a different percentage, the highest percentage indicated in the treatment of T. harzianum by 63.3%. We conclude that T. harzianum better biological control agent base on their ability to colonize all part of seedling and pod. Trichoderma species into the cacao stem, leaf, root and pod allowing systemic colonization of this tissue
Investigation of the Effect of Heat Moisture Treatment on Local Sweet Potato Starch Characteristics
The aimed of this study was to determine the effect of Heat Moisture Treatment (HMT) method on the characteristics of local sweet potato starch. Sweet potato starch were isolated from three varieties, namely based on their flesh colour white, light yellow and purple. The native sweet potato starch clasified into two groups; non HMT groups and HMT groups. The native sweet potato starch with HMT treatment were adjusted to moisture content of 25% and exposed to HMT at 110°C for 3 h. The native and treated starches were characterized for starch physicochemical characteristics espescially total dietary fibre (TDF) which is increased significatl
Effect of Corn Extract and Solution of PEG to Increase Viability and Vigour of Sweet Corn Seed Deterioration at Different Incubation Periods
This study aims to determine the combination of young corn extract and PEG with an incubation period of seed viability and vigor of the sweet corn expired. The study carried out at the Laboratory of Seed Science and Technology Faculty of Agriculture, Syiah Kuala University, Darussalam-Banda Aceh, which lasted from December 2012 to July 2013. Materials used in this study is Expired sweet corn seed with 50% germination. Other materials used are corn extract, PEG 6000, and distilled water. This research used Completely Randomized Design (CRD) factorial 4 x 3, so there are 12 combinations of treatments. Each repeated three times, in order to obtain 36 units of the experiment. Young corn extract concentration factor and solution PEG-2 bar consists of 4 levels, namely: control, 5% + - 2Ψ , 10% + - 2Ψ, and 15% + - 2Ψ. Factors incubation period consists of 3 levels : 18 hours, 24 hours, and 30 hours. Variables measured were potential growth, germination, speed of growth, growing unanimity, vigor index and normal seedling dry weight. The results showed that the concentration of young corn extract very significant effect on the potential variables grow, germination, growing simultaneity, speed of growth, vigor index and normal seedling dry weight. The incubation period of very significant effect on germination variables, simultaneity growth, speed of growth and vigor index. The incubation period significantly affect the potential growth and seedling dry weight normal. There is a significant interaction between young corn extract concentration and incubation period of the vigor index variables. Control treatment (water) with an incubation period of 18 hours is recommended as an alternative to improve the viability and seed vigor of sweet corn expired
Screening of Degenerative Diseases and Quality of Life among Elderly People in Posyandu Lansia Medan Amplas Sub-Districts, Medan
The number of elderly people in Indonesia from year to year is increasing in line with increasing life expectancy. The number of elderly people reached 7.56% of the population. Certainly, the increase in the elderly population will be followed by an increased risk for the elderly to suffer from degenerative diseases. This study aimed to assess the role of degenerative disease screening and health counseling to the quality of life of the elderly in the health post (Posyandu Lansia). This study was a descriptive analytic with prospective approach by assessing 100 elderly people. Screenings done for examination of blood pressure, blood sugar level, cholesterol level, uric acid level, and urine protein. Assessment of quality of life done by using questionnaire of WHOQOL. The elderly in Posyandu Lansia were educated in accordance with the results of the screening and followed up for 4 months. Screening results showed that most elderly people had high blood pressure (89% had high systolic blood pressure and 70% had high diastolic blood pressure). As much as 55% of elderly people had cholesterol levels ≥ 200 mg/dl, while blood sugar levels as ≥ 200 mg/dl were 20% of the elderly and only 20% of respondents who had uric acid levels that exceed normal limits (8,5mg/dl). As many as 13 of respondents indicated a positive result in urine protein. Assessment of the quality of life of elderly based on the score at first examination obtained a mean of 88.26 ± 9.1. Regarding general health status, 21% of elderly felt less satisfied, 47% declared that their health status in normal conditions and 32% felt satisfied with their current health status. The score of quality of life after receiving counseling, obtained a mean of 89.31 ± 7.8. Assessment of general health status showed only 11% of elderly declared unsatisfactory health status. Result of t test showed that there was a significant difference between score of quality of life before and after screening degenerative diseases (p0.05). Screening of degenerative diseases showed a majority of the elderly had a potential risk to suffer from degenerative diseases. Prevention through screening and health counseling particularly in Posyandu Lansia can reduce morbidity and mortality in elderly, and therefore it will improve quality of life for the elderl
The use of herbal medicine in children
The herbal medicine has been widely used in children for the treatment of several symptoms and the prevention of diseases before accessing the hospital for professionals help. There are 3 kinds of marketed herbal medicine including empirical based herbal medicine (jamu), standardized herbal medicine (obat herbal terstandar) and clininically tested herbal medicine (fitofarmaka). This study aimed to investigate the utilization of the marketed herbal medicine along with non marketed ones which was known as the Indonesian original herbal medicine (Obat Asli Indonesia, OAI) in children. The cross sectional study was conducted by interviewing 91 parents whose children were hospitalized in RSUD Meuraxa, Banda Aceh. The interview was conducted using a structured questionnaire from 4 to 29 September 2014. The result showed that the Indonesian original herbal medicine was used most frequently followed by herbal (jamu), standardized herbal and clininically tested herbal medicine. The utilization of herbal medicine was associated with the knowledge of parents and did not correlate to their economic level. All of the parents did not know the side effects of herbal medicine to their children’s body and few of them knew the indication of herbal remedies. Overall, it was concluded that eventhough the knowledge of parents about the safety and activity of herbal medicine was not sufficient the use of herbal medicine in children was commo