Culture of human cell lines by a pathogen-inactivated human platelet lysate

Alternatives to the use of fetal bovine serum (FBS) have been investigated to ensure xeno-free growth condition. In this study we evaluated the efficacy of human platelet lysate (PL) as a substitute of FBS for the in vitro culture of some human cell lines. PL was obtained by pools of pathogen inactivated human donor platelet (PLT) concentrates. Human leukemia cell lines (KG-1, K562, JURKAT, HL-60) and epithelial tumor cell lines (HeLa and MCF-7) were cultured with either FBS or PL. Changes in cell proliferation, viability, morphology, surface markers and cell cycle were evaluated for each cell line. Functional characteristics were analysed by drug sensitivity test and cytotoxicity assay. Our results demonstrated that PL can support growth and expansion of all cell lines, although the cells cultured in presence of PL experienced a less massive proliferation compared to those grown with FBS. We found a comparable percentage of viable specific marker-expressing cells in both conditions, confirming lineage fidelity in all cultures. Functionality assays showed that cells in both FBS- and PL-supported cultures maintained their normal responsiveness to adriamycin and NK cell-mediated lysis. Our findings indicate that PL is a feasible serum substitute for supporting growth and propagation of haematopoietic and epithelial cell lines with many advantages from a perspective of process standardization, ethicality and product safety

Two-year observations of the Jupiter polar regions by JIRAM on board Juno

We observed the evolution of Jupiter's polar cyclonic structures over two years between February 2017 and February 2019, using polar observations by the Jovian InfraRed Auroral Mapper, JIRAM, on the Juno mission. Images and spectra were collected by the instrument in the 5‐μm wavelength range. The images were used to monitor the development of the cyclonic and anticyclonic structures at latitudes higher than 80° both in the northern and the southern hemispheres. Spectroscopic measurements were then used to monitor the abundances of the minor atmospheric constituents water vapor, ammonia, phosphine and germane in the polar regions, where the atmospheric optical depth is less than 1. Finally, we performed a comparative analysis with oceanic cyclones on Earth in an attempt to explain the spectral characteristics of the cyclonic structures we observe in Jupiter's polar atmosphere

Prioritization of high-cost new drugs for HCV: making sustainability ethical

Hepatitis C virus (HCV) infection is a major health problem worldwide. Chronic HCV infection may in the long run cause cirrhosis, hepatic decompensation and hepatocellular carcinoma, with an ultimate disease burden of at least 350,000 deaths per year worldwide. The new generation of highly effective direct acting antivirals (DAA) to treat HCV infection brings major promises to infected patients in terms of exceedingly high rates of sustained virological response (SVR) but also of tolerability, allowing even the sickest patients to be treated. Even in the face of the excellent safety and efficacy and wide theoretical applicability of these regimens, their introduction is currently facing cost and access issues denying their use to many patients in need. Health systems in all countries are facing a huge problem of distributive justice, since while they should guarantee individual rights, among which the right to health in its broader sense, therefore not limited to healing, but extended to quality of life, they must also grant equal access to the healthcare resources and keep the distribution system sustainable. In the face of a disease with a relatively unpredictable course, where many but not of all chronically infected will eventually die of liver disease, selective allocation of this costly resource is debatable. In most countries the favorite solution has been a stratification of patients for prioritization of treatment, which means allowing Interferon-free DAA treatment only in patients with advanced fibrosis or cirrhosis, while keeping on hold persons with lesser stages of liver disease. In this report, we will perform an ethical assessment addressing the issues linked to access to new therapies, prioritization and eligibility criteria, analyzing the meaning of the term “distributive justice” and the different approaches that can guide us (individualistic libertarianism, social utilitarianism and egalitarianism) on this specific matter. Even if over time the price of new DAA will be reduced through competition and eventual patent expiration, the phenomenon of high drug costs will go on in the next decades and we need adequate tools to face the problems of distributive justice that come with it

Bottom-up meta-modelling: An interactive approach

The final publication is available at Springer via http://dx.doi.org/10.1007/978-3-642-33666-9_2Proceedings of 15th International Conference, MODELS 2012, Innsbruck, Austria, September 30–October 5, 2012The intensive use of models in Model-Driven Engineering (MDE) raises the need to develop meta-models with different aims, like the construction of textual and visual modelling languages and the specification of source and target ends of model-to-model transformations. While domain experts have the knowledge about the concepts of the domain, they usually lack the skills to build meta-models. These should be tailored according to their future usage and specific implementation platform, which demands knowledge available only to engineers with great expertise in MDE platforms. These issues hinder a wider adoption of MDE both by domain experts and software engineers. In order to alleviate this situation we propose an interactive, iterative approach to meta-model construction enabling the specification of model fragments by domain experts, with the possibility of using informal drawing tools like Dia. These fragments can be annotated with hints about the intention or needs for certain elements. A meta-model is automatically induced, which can be refactored in an interactive way, and then compiled into an implementation meta-model using profiles and patterns for different platforms and purposes.This work was funded by the Spanish Ministry of Economy and Competitivity (project “Go Lite” TIN2011-24139) and the R&D programme of the Madrid Region (project “e-Madrid” S2009/TIC-1650

Energy/Environment Models: Relationship to Planning in Wisconsin, GDR, Rhone Alps

This report is a description and cross-regional comparison of the institutional structures and modeling methodologies of the three regions participating in the IIASA Research Program on Management of Regional Energy/Environment Systems. Descriptions are presented for the state of Wisconsin (USA), the German Democratic Republic, and the Rhone-Alpes Region (France), by specialists and policy makers from the respective regions. These descriptions demonstrate quite vividly the relationships between the institutional structure of a region and its use of models and planning tools

A Comparison of Model Migration Tools

International audienceModelling languages and thus their metamodels are subject to change. When a metamodel evolves, existing models may no longer conform to the evolved metamodel. To avoid rebuilding them from scratch, existing models must be migrated to conform to the evolved metamodel. Manually migrating existing models is tedious and errorprone. To alleviate this, several tools have been proposed to build a migration strategy that automates the migration of existing models. Little is known about the advantages and disadvantages of the tools in different situations. In this paper, we thus compare a representative sample of migration tools - AML, COPE, Ecore2Ecore and Epsilon Flock - using common migration examples. The criteria used in the comparison aim to support users in selecting the most appropriate tool for their situation

Characterization of Mesoscale Waves in the Jupiter NEB by Jupiter InfraRed Auroral Mapper on board Juno

In 2017, the Jupiter InfraRed Auroral Mapper (JIRAM), on board the NASA-ASI Juno mission, observed a wide longitude region (50° W–80° E in System III) that was perturbed by a wave pattern centered at 15° N in the Jupiter's North Equatorial Belt (NEB). We analyzed JIRAM data acquired on 2017 July 10 using the M-channel and on 2017 February 2 with the spectrometer. The two observations occurred at different times and at slightly different latitudes. The waves appear as clouds blocking the deeper thermal emission. The wave crests are oriented north–south, and the typical wave packet contains 10 crests and 10 troughs. We used Fourier analysis to rigorously determine the wavenumbers associated with the observed patterns at a confidence level of 90%. Wavelet analysis was also used to constrain the spatial localization of the largest energies involved in the process and determine the wavelengths carrying the major contribution. We found wavelengths ranging from 1400 to 1900 km, and generally decreasing toward the west. Where possible, we also computed a vertical location of the cloud pressure levels from the inversion of the spectral radiances measured by the JIRAM spectrometer. The waves were detected at pressure levels consistent with the NH3 as well as NH4SH clouds. Phase velocities could not be determined with sufficient confidence to discriminate whether the alternating crests and troughs are a propagating wave or a manifestation of a fluid dynamical instability

Klebsiella pneumoniae bacteraemia complicating rotavirus gastroenteritis in two infants with glucocorticoid deficiency

Rotavirus gastroenteritis was complicated by Klebsiella Pneumoniae bacteraemia in two infants with glucocorticoid deficient conditions who were treated with 'stress dose' hydrocortisone during their illness. Delayed healing in the context of glucocorticoid administration combined with damage from rotavirus infection may result in increased risk of mucosal invasion by gastrointestinal bacteria and subsequent enteric gram-negative bacteraemia