In Vivo Analysis Of Angiotensin II-Induced Hypertension And Vascular Disease In Rats

Murine models of abdominal aortic aneurysms (AAA) are commonly used to study the pathogenesis of this disease. Rats are often used in laboratory studies because of their size and because they are more physiologically similar to humans than mice are. In this study, we subcutaneously implanted 9-week-old apolipoprotein E-deficient (apoE KO) rats with angiotensin II-filled pumps to study the effects of this vasoconstricting hormone on aneurysm development. Deletion of the apoE gene in mice causes excess lipid accumulation in the blood vessels, thereby increasing the likelihood of atherosclerotic lesions in the aorta. However, atherosclerotic plaque buildup was not evident when the rats consumed either normal chow or high fat diets, and no dissecting aneurysms were identified via ultrasound. We observed an average of 32.5 +/- 28.8 mmHg increase in systolic blood pressures as early as 4 days post-pump implantation and an increase of 54.2 +/- 25.9 mmHg from baseline 6 weeks after angiotensin II infusion began. This study shows that apoE KO rats can be valuable models for the physiological development of hypertension but not for dissecting suprarenal aneurysms. Future studies will focus on studying the effects of angiotensin II on the hearts of these genetically modified animals

Prenatal Care Cards: Imparting Patient Autonomy During Pregnancy.

Introduction/Problem: Indiana University Student Outreach Clinic (IU-SOC) has been providing medical care to vulnerable populations for the past 12 years. In that time, students have witnessed barriers to care impacting the neighbors in the community, including lack of health insurance due to financial limitations or limited residency documentation. These barriers are particularly burdensome to pregnant patients, and the bimonthly Prenatal Clinic was created five years ago to address this need. A major limitation is ensuring effective communication between the IU-SOC Electronic Medical Record (EMR), Practice Fusion, and the multiple health systems in the community. Intervention: After reviewing the Pan American Health Organization (P.A.H.O) recommendations for similar communities, we created a tool that can be utilized at all outreach clinics: the prenatal care card. These care cards contain all relevant prenatal information such as first, second, and third trimester prenatal labs, blood pressure measurements, fetal anatomy ultrasound results, and other tests that will allow providers to establish baseline values that determine future care. The card can be easily translated for improved clarity and is printed on sturdy, laminated cardstock. IU-SOC contact information is included on the bottom of the card in case future providers have concerns, and patients receive a photocopy of the card for safekeeping. Our copy is uploaded to Practice Fusion for future reference at IU-SOC. Conclusion: The IU-SOC prenatal care card has been implemented as a way to communicate between the clinic and other healthcare providers, in accordance with P.A.H.O. standards of perinatal care. Since September 2021, four IU-SOC patients have been offered the prenatal care card and have expressed overall satisfaction so far. The value of the prenatal card is three-fold: to provide patients with ownership of their medical information, to be easily shareable with any medical center, and to enhance their pregnancy experience overall. The utility of the prenatal card will be further established as more pregnant patients are provided this tool

Toward Automation of the Supine Pressor Test for Preeclampsia

Preeclampsia leads to increased risk of morbidity and mortality for both mother and fetus. Most previous studies have largely neglected mechanical compression of the left renal vein by the gravid uterus as a potential mechanism. In this study, we first used a murine model to investigate the pathophysiology of left renal vein constriction. The results indicate that prolonged renal vein stenosis after 14 days can cause renal necrosis and an increase in blood pressure (BP) of roughly 30 mmHg. The second part of this study aimed to automate a diagnostic tool, known as the supine pressor test (SPT), to enable pregnant women to assess their preeclampsia development risk. A positive SPT has been previously defined as an increase of at least 20 mmHg in diastolic BP when switching between left lateral recumbent and supine positions. The results from this study established a baseline BP increase between the two body positions in nonpregnant women and demonstrated the feasibility of an autonomous SPT in pregnant women. Our results demonstrate that there is a baseline increase in BP of roughly 10-14 mmHg and that pregnant women can autonomously perform the SPT. Overall, this work in both rodents and humans suggests that (1) stenosis of the left renal vein in mice leads to elevation in BP and acute renal failure, (2) nonpregnant women experience a baseline increase in BP when they shift from left lateral recumbent to supine position, and (3) the SPT can be automated and used autonomously

Does academic assessment system type affect levels of academic stress in medical students? A cross-sectional study from Pakistan

Introduction Stress among medical students induced by academic pressures is on the rise among the student population in Pakistan and other parts of the world. Our study examined the relationship between two different systems employed to assess academic performance and the levels of stress among students at two different medical schools in Karachi, Pakistan. Methods A sample consisting of 387 medical students enrolled in pre-clinical years was taken from two universities, one employing the semester examination system with grade point average (GPA) scores (a tiered system) and the other employing an annual examination system with only pass/fail grading. A pre-designed, self-administered questionnaire was distributed. Test anxiety levels were assessed by The Westside Test Anxiety Scale (WTAS). Overall stress was evaluated using the Perceived Stress Scale (PSS). Results There were 82 males and 301 females while four did not respond to the gender question. The mean age of the entire cohort was 19.7±1.0 years. A total of 98 participants were from the pass/fail assessment system while 289 were from the GPA system. There was a higher proportion of females in the GPA system (85% vs. 59%; p \u3c 0.01). Students in the pass/fail assessment system had a lower score on the WTAS (2.4±0.8 vs. 2.8±0.7; p=0.01) and the PSS (17.0±6.7 vs. 20.3±6.8; p \u3c 0.01), indicating lower levels of test anxiety and overall stress than in students enrolled in the GPA assessment system. More students in the pass/fail system were satisfied with their performance than those in the GPA system. Conclusion Based on the present study, we suggest governing bodies to revise and employ a uniform assessment system for all the medical colleges to improve student academic performance and at the same time reduce stress levels. Our results indicate that the pass/fail assessment system accomplishes these objectives

Dietary habits and physical activity patterns in relation to nutritional status among school-aged children in Pakistan: A cross-sectional study

Background: Childhood malnutrition remains a significant public health problem impacting the physical and mental growth if school aged children, particularly in limited-resource countries. Objective: The study objective was to assess levels of physical activity, patterns of screen time (S.T.), the relationship between physical activity and screen time patterns, and how these factors affect growth status (adjusting for socioeconomic status). Methodology: A cross-sectional study included 3,834 children between 6–14 years attending pre-selected schools. Teachers, students, and parents were invited to fill out a standardized questionnaire, and Body Mass Index (BMI) was calculated using Center for disease control (CDC) centile charts. A Chi-square was performed to see the possible association between any height and weight abnormalities and all possible risk factors. Multivariate logistic regression was applied to see the effect of variables significantly associated with univariate analysis. Results: Approximately 2,447 (63.8%) children were between 11–14 years old and 1,387 (36.2%) were between 4–10 years old. The mean height was 143.71 ± 16.51 centimetres, the mean weight was 36.5 ± 12.9 kilogram, and the mean BMI was 17.16 ± 3.52. Multivariate logistic regression status and junk food combined affected stunting socioeconomic status was significantly associated with being underweight p = 0.001. Conclusion: Childhood obesity and stunting remain significant problems in Pakistani school-going children. These are significantly associated with poverty, a lack of physical activity opportunities, and available food quality

Automation of the Supine Pressor Test for Preeclampsia

Preeclampsia leads to increased risk of morbidity and mortality for both mother and fetus. Most previous studies have largely neglected mechanical compression of the left renal vein by the gravid uterus as a potential mechanism. In this study we first used a murine model to investigate the pathophysiology of left renal vein constriction. The results indicate that prolonged renal vein stenosis after 14 days can cause renal necrosis and an increase in blood pressure (BP) of roughly 30 mmHg. The second part of this study aimed to automate a diagnostic tool, known as the supine pressor test (SPT), to enable pregnant women to assess their preeclampsia development risk. A positive SPT has been previously defined as an increase of at least 20 mmHg in diastolic BP when switching between left lateral recumbent and supine positions. The results from this study established a baseline BP increase between the two body positions in non-pregnant female subjects and demonstrated the feasibility and utility of an automated SPT in pregnant women. Our results demonstrate that there is a baseline increase in BP of roughly 10-14 mmHg and that pregnant women can autonomously perform the SPT. Overall, this work in both rodents and humans suggests that 1) stenosis of the left renal vein in mice leads to elevation in BP and acute renal failure, 2) non-pregnant women experience a baseline increase in BP when they shift from left lateral recumbent to supine position, and 3) the SPT can be automated and used autonomously

Design of Mega LEO Constellations for Continuous Coverage over Pakistan: Satellite Communication

Satellite communication was effectively done in Geostationary Earth Orbit (GEO) in the past years. Recently the trend has shifted from GEO to Low Earth Orbit (LEO). The objective of our study is to propose a satellite constellation for Pakistan in LEO that will provide continuous coverage over Pakistan. As LEO is much closer to the earth as compared to other orbits such as GEO and High Earth Orbit (HEO) etc. one can achieve benefits like low latency rate, less fuel consumption, and signal transmission loss. In ongoing research, an attempt has been made to design the satellite constellation in LEO using the software, System Tool Kit (STK) which has 2D and 3D environment modeling. In the designed constellation, the satellites pass over Pakistan and access the target area. To get uninterrupted continuous coverage, the number of satellites per plane and the number of orbits is increased. The orbital inclinations were also adjusted to achieve the objective. One of the important tasks for continuous coverage is the concept of satellite handshaking which means that soon a satellite gets away from the line of sight of the ground station antenna; another satellite comes within the line of sight of that antenna. LEO satellites are more favorable for communication purposes as they provide reliable communication as well as higher bandwidth

Design of Mega LEO Constellations for Continuous Coverage over Pakistan: Satellite Communication

Satellite communication was effectively done in Geostationary Earth Orbit (GEO) in the past years. Recently the trend has shifted from GEO to Low Earth Orbit (LEO). The objective of our study is to propose a satellite constellation for Pakistan in LEO that will provide continuous coverage over Pakistan. As LEO is much closer to the earth as compared to other orbits such as GEO and High Earth Orbit (HEO) etc. one can achieve benefits like low latency rate, less fuel consumption, and signal transmission loss. In ongoing research, an attempt has been made to design the satellite constellation in LEO using the software, System Tool Kit (STK) which has 2D and 3D environment modeling. In the designed constellation, the satellites pass over Pakistan and access the target area. To get uninterrupted continuous coverage, the number of satellites per plane and the number of orbits is increased. The orbital inclinations were also adjusted to achieve the objective. One of the important tasks for continuous coverage is the concept of satellite handshaking which means that soon a satellite gets away from the line of sight of the ground station antenna; another satellite comes within the line of sight of that antenna. LEO satellites are more favorable for communication purposes as they provide reliable communication as well as higher bandwidth

An Empirical Investigation on Plastic Waste Issues and Plastic Disposal Strategies to Protect the Environment: A UAE Perspective

Due to the rise in waste, both developed and developing countries’ municipal solid waste (MSW) disposal systems are overburdened. The increased inflow of used plastic water bottles has rendered the situation difficult. Plastic usage in several forms is becoming indispensable in daily life, despite its harmful effects on humans and animals in general. Government bodies are eager to cut plastic usage in several forms, but the manufacturing costs and selling prices make it difficult to replace plastic with materials that are safe for living bodies. One such problem lies in the form of the water bottle. The use of plastic in water bottles is much more common in Gulf countries because of their geographical position and the scarcity of potable water. The large amount of plastic waste created by used water bottles is a very critical issue, as it has a severe environmental effect and is a hurdle in efforts to foster sustainable development and green initiatives. The present research undertook an empirical investigation on plastic waste issues, environmental concerns, and various mitigating initiatives. It further investigated the waste management system using closed-loop recycling for water bottles as an efficient means of managing MSW and protecting the environment in the UAE. A survey was initiated and distributed among the UAE population to study the feasibility of building such a system. The responses to the plastic waste issue and mitigating solutions were found to be encouraging. The statistical analysis showed a higher mean value of up to 4.47 on the 5-point Likert scale