CFD Modelling of Micromixing in a T-mixer with Square Bends

International audienceCFD simulations are performed to study the micro-mixing in a T-mixer with square bends by using the Villermaux-Dushman reaction. The calculated segregation indices are compared with previously published data and it is shown that whilst the trend is capture the simulation overpredict the extent of miccromixing. This paper provides guidance on the computational requirements needed to obtain accurate results in such laminar flows and shows the complex species fields that develop within the reactor

Characterization of micromixing in a Continuous Oscillatory Baffled Reactor

International audienceMicromixing is the limiting step in the progress of fast reactions and fast crystallization processes. Micromixing performance of a Nitech© glass COBR has been characterized using the competing parallel iodide-iodate test reactions. Macromixing length was also qualitatively determined by visualization of a tracer. The results show that an increase in the amplitude to baffle space ratio and a decrease of oscillation frequency improves mixing performance

UniProtKB amid the turmoil of plant proteomics research.

The UniProt KnowledgeBase (UniProtKB) provides a single, centralized, authoritative resource for protein sequences and functional information. The majority of its records is based on automatic translation of coding sequences (CDS) provided by submitters at the time of initial deposition to the nucleotide sequence databases (INSDC). This article will give a general overview of the current situation, with some specific illustrations extracted from our annotation of Arabidopsis and rice proteomes. More and more frequently, only the raw sequence of a complete genome is deposited to the nucleotide sequence databases and the gene model predictions and annotations are kept in separate, specialized model organism databases (MODs). In order to be able to provide the complete proteome of model organisms, UniProtKB had to implement pipelines for import of protein sequences from Ensembl and EnsemblGenomes. A single genome can be the target of several unrelated sequencing projects and the final assembly and gene model predictions may diverge quite significantly. In addition, several cultivars of the same species are often sequenced - 1001 Arabidopsis cultivars are currently under way - and the resulting proteomes are far from being identical. Therefore, one challenge for UniProtKB is to store and organize these data in a convenient way and to clearly defined reference proteomes that should be made available to users. Manual annotation is one of the landmarks of the Swiss-Prot section of UniProtKB. Besides adding functional annotation, curators are checking, and often correcting, gene model predictions. For plants, this task is limited to Arabidopsis thaliana and Oryza sativa subsp. japonica. Proteomics data providing experimental evidences confirming the existence of proteins or identifying sequence features such as post-translational modifications are also imported into UniProtKB records and the knowledgebase is cross-referenced to numerous proteomics resource

ROBUST TECHNIQUES FOR BUILDING FOOTPRINT EXTRACTION IN AERIAL LASER SCANNING 3D POINT CLOUDS

The building footprint is crucial for a volumetric 3D representation of a building that is applied in urban planning, 3D city modeling, cadastral and topographic map generation. Aerial laser scanning (ALS) has been recognized as the most suitable means of large-scale 3D point cloud data (PCD) acquisition. PCD can produce geometric detail of a scanned surface. However, it is almost impossible to get point clouds without noise and outliers. Besides, data incompleteness and occlusions are two common phenomena for PCD. Most of the existing methods for building footprint extraction employ classification, segmentation, voting techniques (e.g., Hough-Transform or RANSAC), or Principal Component Analysis (PCA) based methods. It is known that classical PCA is highly sensitive to outliers, even RANSAC which is known as a robust technique for shape detection is not free from outlier effects. This paper presents a novel algorithm that employs MCMD (maximum consistency within minimum distance), MSAC (a robust variant of RANSAC) and a robust regression to extract reliable building footprints in the presence of outliers, missing points and irregular data distributions. The algorithm is successfully demonstrated through two sets of ALS PCD

Impact of thixotropy on flow patterns induced in a stirred tank : numerical and experimental studies

Agitation of a thixotropic shear-thinning fluid exhibiting a yield stress is investigated both experimentally and via simulations. Steady-state experiments are conducted at three impeller rotation rates (1, 2 and 8 s−1) for a tank stirred with an axial-impeller and flow-field measurements are made using particle image velocimetry (PIV) measurements. Threedimensional numerical simulations are also performed using the commercial CFD code ANSYS CFX10.0. The viscosity of the suspension is determined experimentally and is modelled using two shear-dependant laws, one of which takes into account the flow instabilities of such fluids at low shear rates. At the highest impeller speed, the flow exhibits the familiar outward pumping action associated with axial-flow impellers. However, as the impeller speed decreases, a cavern is formed around the impeller, the flow generated in the vicinity of the agitator reorganizes and its pumping capacity vanishes. An unusual flow pattern, where the radial velocity dominates, is observed experimentally at the lowest stirring speed. It is found to result from wall slip effects. Using blades with rough surfaces prevents this peculiar behaviour and mainly resolves the discrepancies between the experimental and computational results

Psip1/Ledgf p75 restrains<i>Hox</i>gene expression by recruiting both trithorax and polycomb group proteins

Trithorax and polycomb group proteins are generally thought to antagonize one another. The trithorax familymember MLL (myeloid/lymphoid or mixedlineage leukemia) is presumed to activate Hox expression, counteracting polycomb-mediated repression. PC4 and SF2 interacting protein 1 (PSIP1)/p75, also known as LEDGF, whose PWWP domain binds to H3K36me3, interacts with MLL and tethers MLL fusion proteins toHOXA9 in leukaemias. Here we show, unexpectedly, that Psip1/p75 regulates homeotic genes by recruiting not only MLL complexes, but also the polycomb group protein Bmi1. In Psip1-/- cells binding of Mll1/2, Bmi1 and the co-repressor Ctbp1 at Hox loci are all abrogated and Hoxa and Hoxd mRNA expression increased. Our data not only reveal a potential mechanism of action for Psip1 in the regulation of Hox genes but also suggest an unexpected interplay between proteins usually considered as transcriptional activators and repressors. © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research

The Geysers Geothermal Field Update1990/2010

In this report, we have presented data in four sections: (1) THE GEYSERS HISTORICAL UPDATE 1990-2010 - A historical update of the primary developments at The Geysers between 1990 and 2010 which uses as its start point Section IIA of the Monograph - 'Historical Setting and History of Development' that included articles by James Koenig and Susan Hodgson. (2) THE GEYSERS COMPREHENSIVE REFERENCE LIST 1990-2010 - In this section we present a rather complete list of technical articles and technical related to The Geysers that were issued during the period 1990-2010. The list was compiled from many sources including, but not limited to scientific journals and conference proceedings. While the list was prepared with care and considerable assistance from many geothermal colleagues, it is very possible that some papers could have been missed and we apologize to their authors in advance. The list was subdivided according to the following topics: (1) Field characterization; (2) Drilling; (3) Field development and management; (4) Induced seismicity; (5) Enhanced Geothermal Systems; (6) Power production and related issues; (7) Environment-related issues; and (8) Other topics. (3) GRC 2010 ANNUAL MEETING GEYSERS PAPERS - Included in this section are the papers presented at the GRC 2010 Annual Meeting that relate to The Geysers. (4) ADDITIONAL GEYSERS PAPERS 1990-2010 - Eighteen additional technical papers were included in this publication in order to give a broad background to the development at The Geysers after 1990. The articles issued during the 1990-2010 period were selected by colleagues considered knowledgeable in their areas of expertise. We forwarded the list of references given in Section 2 to them asking to send us with their selections with a preference, because of limited time, to focus on those papers that would not require lengthy copyright approval. We then chose the articles presented in this section with the purpose of providing the broadest possible view across all technical fields, as related to The Geysers steam-dominated geothermal system. The Geysers has seen many fundamental changes between 1990-2010 and yet the geothermal resource seems still to be robust to the extent that, long after its anticipated life span, we are seeing new geothermal projects being developed on the north and west peripheries of the field. It is hoped that this report provides a focused data source particularly for those just starting their geothermal careers, as well as those who have been involved in the interesting and challenging field of geothermal energy for many years. Despite many hurdles The Geysers has continued to generate electrical power for 50 years and its sustainability has exceeded many early researchers expectations. It also seems probable that, with the new projects described above, generation will continue for many years to come. The success of The Geysers is due to the technical skills and the financial acumen of many people, not only over the period covered by this report (1990-2010), but since the first kilowatt of power was generated in 1960. This Special Report celebrates those 50 years of geothermal development at The Geysers and attempts to document the activities that have brought success to the project so that a permanent record can be maintained. It is strongly hoped and believed that a publication similar to this one will be necessary in another 20 years to document further activities in the field

Compliance with mandatory standards in agriculture : a comparative approach of the EU vis-à-vis the United States, Canada and New Zealand

This report presents some of the interim results of the project 'Facilitating the CAP reform: Compliance and competitiveness of European agriculture'. It summarises and integrates the implementation of cross compliance measures in seven EU countries (France, Germany, Italy, Netherlands, United Kingdom, Spain and Poland), with a particular focus on the degree of compliance and the costs of compliance. Also, the implementation of similar measures is examined in three non-EU countries (Canada, United States and New Zealand)

Exploring the synergies between cross compliance and certification schemes

This report presents some of the interim results of the project 'Facilitating the CAP reform: Compliance and competitiveness of European agriculture'. It examines the similarities and differences between mandatory cross compliance standards and those set by voluntary certification schemes. There is a potential synergy between cross compliance and certification schemes, not least because both approaches set minimum standards and enforce those standards through inspection systems. Although there are some strong limitations, there is sufficient overlap in the standards set and in approaches to control to warrant further investigation of the potential for the harmonisation of standards and collaborative approaches to control

Term Matrix: a novel Gene Ontology annotation quality control system based on ontology term co-annotation patterns.

Biological processes are accomplished by the coordinated action of gene products. Gene products often participate in multiple processes, and can therefore be annotated to multiple Gene Ontology (GO) terms. Nevertheless, processes that are functionally, temporally and/or spatially distant may have few gene products in common, and co-annotation to unrelated processes probably reflects errors in literature curation, ontology structure or automated annotation pipelines. We have developed an annotation quality control workflow that uses rules based on mutually exclusive processes to detect annotation errors, based on and validated by case studies including the three we present here: fission yeast protein-coding gene annotations over time; annotations for cohesin complex subunits in human and model species; and annotations using a selected set of GO biological process terms in human and five model species. For each case study, we reviewed available GO annotations, identified pairs of biological processes which are unlikely to be correctly co-annotated to the same gene products (e.g. amino acid metabolism and cytokinesis), and traced erroneous annotations to their sources. To date we have generated 107 quality control rules, and corrected 289 manual annotations in eukaryotes and over 52 700 automatically propagated annotations across all taxa