Crosstalk between C/EBPbeta phosphorylation, arginine methylation, and SWI/SNF/Mediator implies an indexing transcription factor code

Cellular signalling cascades regulate the activity of transcription factors that convert extracellular information into gene regulation. C/EBPbeta is a ras/MAPkinase signal-sensitive transcription factor that regulates genes involved in metabolism, proliferation, differentiation, immunity, senescence, and tumourigenesis. The protein arginine methyltransferase 4 PRMT4/CARM1 interacts with C/EBPbeta and dimethylates a conserved arginine residue (R3) in the C/EBPbeta N-terminal transactivation domain, as identified by mass spectrometry of cell-derived C/EBPbeta. Phosphorylation of the C/EBPbeta regulatory domain by ras/MAPkinase signalling abrogates the interaction between C/EBPbeta and PRMT4/CARM1. Differential proteomic screening, protein interaction studies, and mutational analysis revealed that methylation of R3 constraines interaction with SWI/SNF and Mediator complexes. Mutation of the R3 methylation site alters endogenous myeloid gene expression and adipogenic differentiation. Thus, phosphorylation of the transcription factor C/EBPbeta couples ras signalling to arginine methylation and regulates the interaction of C/EBPbeta with epigenetic gene regulatory protein complexes during cell differentiation

Reducing Data Center Loads for a Large-Scale, Low-Energy Office Building: NREL's Research Support Facility (Book)

This publication detailing the design, implementation strategies, and continuous performance monitoring of NREL's Research Support Facility data center. Data centers are energy-intensive spaces that facilitate the transmission, receipt, processing, and storage of digital data. These spaces require redundancies in power and storage, as well as infrastructure, to cool computing equipment and manage the resulting waste heat (Tschudi, Xu, Sartor, and Stein, 2003). Data center spaces can consume more than 100 times the energy of standard office spaces (VanGeet 2011). The U.S. Environmental Protection Agency (EPA) reported that data centers used 61 billion kilowatt-hours (kWh) in 2006, which was 1.5% of the total electricity consumption in the U.S. (U.S. EPA, 2007). Worldwide, data centers now consume more energy annually than Sweden (New York Times, 2009). Given their high energy consumption and conventional operation practices, there is a potential for huge energy savings in data centers. The National Renewable Energy Laboratory (NREL) is world renowned for its commitment to green building construction. In June 2010, the laboratory finished construction of a 220,000-square-foot (ft{sup 2}), LEED Platinum, Research Support Facility (RSF), which included a 1,900-ft{sup 2} data center. The RSF will expand to 360,000 ft{sup 2} with the opening of an additional wing December, 2011. The project's request for proposals (RFP) set a whole-building demand-side energy use requirement of a nominal 35 kBtu/ft{sup 2} per year. On-site renewable energy generation will offset the annual energy consumption. To support the RSF's energy goals, NREL's new data center was designed to minimize its energy footprint without compromising service quality. Several implementation challenges emerged during the design, construction, and first 11 months of operation of the RSF data center. This document highlights these challenges and describes in detail how NREL successfully overcame them. The IT settings and strategies outlined in this document have been used to significantly reduce data center energy requirements in the RSF; however, these can also be used in existing buildings and retrofits

Definition of a 'Zero Net Energy' Community

This document provides a definition for a net zero-energy community. A community that offsets all of its energy use from renewables available within the community's built environment

First CNGS events detected by LVD

The CERN Neutrino to Gran Sasso (CNGS) project aims to produce a high energy, wide band $\nu_{\mu}$ beam at CERN and send it toward the INFN Gran Sasso National Laboratory (LNGS), 732 km away. Its main goal is the observation of the $\nu_{\tau}$ appearance, through neutrino flavour oscillation. The beam started its operation in August 2006 for about 12 days: a total amount of $7.6~10^{17}$ protons were delivered to the target. The LVD detector, installed in hall A of the LNGS and mainly dedicated to the study of supernova neutrinos, was fully operating during the whole CNGS running time. A total number of 569 events were detected in coincidence with the beam spill time. This is in good agreement with the expected number of events from Montecarlo simulations.Comment: Accepted for publication by the European Physical Journal C ; 7 pages, 11 figure

On-line recognition of supernova neutrino bursts in the LVD detector

In this paper we show the capabilities of the Large Volume Detector (INFN Gran Sasso National Laboratory) to identify a neutrino burst associated to a supernova explosion, in the absence of an "external trigger", e.g., an optical observation. We describe how the detector trigger and event selection have been optimized for this purpose, and we detail the algorithm used for the on-line burst recognition. The on-line sensitivity of the detector is defined and discussed in terms of supernova distance and electron anti-neutrino intensity at the source.Comment: Accepted for pubblication on Astroparticle Physics. 13 pages, 10 figure

Study of the effect of neutrino oscillation on the supernova neutrino signal with the LVD detector

We present an update of our previous study (astro-ph/0112312) on how $\nu$ oscillations affect the signal from a supernova core collapse observed in the LVD detector at LNGS. In this paper we use a recent, more precise determination of the cross section (astro-ph/0302055) to calculate the expected number of inverse beta decay events, we introduce in the simulation also the $\nu$-{\rm Fe} interactions, we include the Earth matter effects and, finally, we study also the inverted mass hierarchy case.Comment: 4 pages, 4 figures, to appear in the Proceedings of ICRC 200

Search for low energy neutrinos in correlation with the 8 events observed by the EXPLORER and NAUTILUS detectors in 2001

We report on a search for low-energy neutrino (antineutrino) bursts in correlation with the 8 time coincident events observed by the gravitational waves detectors EXPLORER and NAUTILUS (GWD) during the year 2001. The search, conducted with the LVD detector (INFN Gran Sasso National Laboratory, Italy), has considered several neutrino reactions, corresponding to different neutrino species, and a wide range of time intervals around the (GWD) observed events. No evidence for statistically significant correlated signals in LVD has been found. Assuming two different origins for neutrino emission, the cooling of a neutron star from a core-collapse supernova or from coalescing neutron stars and the accretion of shocked matter, and taking into account neutrino oscillations, we derive limits to the total energy emitted in neutrinos and to the amount of accreting mass, respectively.Comment: Accepted for publication in Astronomy and Astrophysic

Pedestrian Road Traffic Injuries in Urban Peruvian Children and Adolescents: Case Control Analyses of Personal and Environmental Risk Factors

BACKGROUND: Child pedestrian road traffic injuries (RTIs) are an important cause of death and disability in poorer nations, however RTI prevention strategies in those countries largely draw upon studies conducted in wealthier countries. This research investigated personal and environmental risk factors for child pedestrian RTIs relevant to an urban, developing world setting. METHODS: This is a case control study of personal and environmental risk factors for child pedestrian RTIs in San Juan de Miraflores, Lima, Perú. The analysis of personal risk factors included 100 cases of serious pedestrian RTIs and 200 age and gender matched controls. Demographic, socioeconomic, and injury data were collected. The environmental risk factor study evaluated vehicle and pedestrian movement and infrastructure at the sites in which 40 of the above case RTIs occurred and 80 control sites. FINDINGS: After adjustment, factors associated with increased risk of child pedestrian RTIs included high vehicle volume (OR 7.88, 95%CI 1.97-31.52), absent lane demarcations (OR 6.59, 95% CI 1.65-26.26), high vehicle speed (OR 5.35, 95%CI 1.55-18.54), high street vendor density (OR 1.25, 95%CI 1.01-1.55), and more children living in the home (OR 1.25, 95%CI 1.00-1.56). Protective factors included more hours/day spent in school (OR 0.52, 95%CI 0.33-0.82) and years of family residence in the same home (OR 0.97, 95%CI 0.95-0.99). CONCLUSION: Reducing traffic volumes and speeds, limiting the number of street vendors on a given stretch of road, and improving lane demarcation should be evaluated as components of child pedestrian RTI interventions in poorer countries

Study of the effect of neutrino oscillations on the supernova neutrino signal in the LVD detector

The LVD detector, located in the INFN Gran Sasso National Laboratory (Italy), studies supernova neutrinos through the interactions with protons and carbon nuclei in the liquid scintillator and interactions with the iron nuclei of the support structure. We investigate the effect of neutrino oscillations in the signal expected in the LVD detector. The MSW effect has been studied in detail for neutrinos travelling through the collapsing star and the Earth. We show that the expected number of events and their energy spectrum are sensitive to the oscillation parameters, in particular to the mass hierarchy and the value of $\theta_{13}$, presently unknown. Finally we discuss the astrophysical uncertainties, showing their importance and comparing it with the effect of neutrino oscillations on the expected signal.Comment: Accepted for pubblication on Astroparticle Physics. 36 pages, 18 figure