12 research outputs found
Prediction of climate change effects on the runoff regime of a forested catchment in northern Iran
Asymptotic properties of the sample mean in adaptive stratified sequential sampling with multiple selection criteria
We extend the method of adaptive two-stage sequen-tial sampling to include designs where there is more than one crite-ria used in deciding on the allocation of additional sampling effort. These criteria, or conditions, can be a measure of the target popula-tion, or a measure of some related population. We develop Murthy estimator for the design that is unbiased estimators for the pop-ulation mean, and propose another, more efficient, estimator. We investigate asymptotic properties of this estimator. We use a sim-ulation study to investigate design properties of the multi-criteria adaptive stratified sequential sampling scheme and also some esti-mator properties under the design.Scopu
Profiling structurally deficient bridges in the United States: Sampling bridge performance data for qualitative trends
J. Biol. Chem.
The laminin (LN) family of large heterotrimeric extracellular matrix glycoproteins has multiple functions: LNs take part in the regulation of processes such as cell migration, differentiation, and proliferation, in addition to contributing to the structure of basement membranes. LN-10, composed of alpha5, beta1, and gamma1 chains, is widely distributed in most basement membranes of both epithelia and endothelia. We determined the complete human cDNA sequence for the LN a5 chain and produced recombinant human LN-10 (rLN-10) in HEK293 cells by triple transfection of full-length cDNAs encoding the human LN alpha5, beta1, and gamma1 chains. The rLN-10 was purified using affinity chromatography and had an apparent molecular mass of -800 kDa in SDS-PAGE and a native domain structure in rotary shadowing electron microscopy. By using function- blocking monoclonal antibodies, integrin asp, was found to be a major mediator of adhesion of HT-1080 and human saphenous vein endothelial cells. Human saphenous vein endothelial cells adhered more strongly to rLN-10 than to LN-1 and LN-8 and showed better migration on rLN-10, compared with several other matrices. Considering the cell adhesive and migration-promoting properties of rLN-10 on endothelial cells, this molecule could be useful in improving the biocompatibility and endothelialization of vascular grafts
Attenuating effect of α‐pinene on neurobehavioural deficit, oxidative damage and inflammatory response following focal ischaemic stroke in rat
Recombinant human laminin-10 (alpha 5,beta 1,gamma 1) - Production, purification, and migration-promoting activity on vascular endothelial cells
The laminin (LN) family of large heterotrimeric extracellular matrix glycoproteins has multiple functions: LNs take part in the regulation of processes such as cell migration, differentiation, and proliferation, in addition to contributing to the structure of basement membranes. LN-10, composed of alpha5, beta1, and gamma1 chains, is widely distributed in most basement membranes of both epithelia and endothelia. We determined the complete human cDNA sequence for the LN a5 chain and produced recombinant human LN-10 (rLN-10) in HEK293 cells by triple transfection of full-length cDNAs encoding the human LN alpha5, beta1, and gamma1 chains. The rLN-10 was purified using affinity chromatography and had an apparent molecular mass of -800 kDa in SDS-PAGE and a native domain structure in rotary shadowing electron microscopy. By using function- blocking monoclonal antibodies, integrin asp, was found to be a major mediator of adhesion of HT-1080 and human saphenous vein endothelial cells. Human saphenous vein endothelial cells adhered more strongly to rLN-10 than to LN-1 and LN-8 and showed better migration on rLN-10, compared with several other matrices. Considering the cell adhesive and migration-promoting properties of rLN-10 on endothelial cells, this molecule could be useful in improving the biocompatibility and endothelialization of vascular grafts