Speedy Techniques to Evaluate Seismic Site Effects in Particular Geomorphologic Conditions: Faults, Cavities, Landslides and Topographic Irregularities

The ground motion that can be recorded at the free surface of a terrain is the final result of a series of phenomena that can be grouped into three fundamental typologies: the source mechanism, the seismic wave propagation till the bedrock interface below the investigated site and the site effects (Fig. 1). The first two features define the kind of seismic input whereas the third represents all modifications that can occur as a consequence of the interaction between seismic waves and local characteristics of the investigated site. The physical and mechanical properties of terrains as well as their morphologic and stratigraphic features appreciably affect the characteristics of the ground motion observed at the surface. The whole process of modifications undergone by a given seismic input in terms of amplitude, frequency content and duration, as a consequence of local characteristics, is generally termed the “local seismic response”. It is indeed well known that the spectral composition of a seismic event is modified first during the source-bedrock path (attenuation function), and second, when the seismic input interacts with the soft terrains layered between the bedrock and the free surface (Fig. 1a). This latter effect, significantly changes the spectral content so that it is extremely important for estimating the final input to which all structures built in the study area will be subjected.peer-reviewe

Blocking CD248 molecules in perivascular stromal cells of patients with systemic sclerosis strongly inhibits their differentiation toward myofibroblasts and proliferation: A new potential target for antifibrotic therapy

Background: Fibrosis may be considered the hallmark of systemic sclerosis (SSc), the end stage triggered by different pathological events. Transforming growth factor-β (TGF-β) and platelet-derived growth factor BB (PDGF-BB) are profibrotic molecules modulating myofibroblast differentiation and proliferation, respectively. There is evidence linking CD248 with these two molecules, both highly expressed in patients with SSc, and suggesting that CD248 may be a therapeutic target for several diseases. The aim of this work was to evaluate the expression of CD248 in SSc skin and its ability to modulate SSc fibrotic process. Methods: After ethical approval was obtained, skin biopsies were collected from 20 patients with SSc and 10 healthy control subjects (HC). CD248 expression was investigated in the skin, as well as in bone marrow mesenchymal stem cells (MSCs) treated with TGF-β or PDGF-BB, by immunofluorescence, qRT-PCR, and Western blotting. Finally, in SSc-MSCs, the CD248 gene was silenced by siRNA. Results: Increased expression of CD248 was found in endothelial cells and perivascular stromal cells of SSc skin. In SSc-MSCs, the levels of CD248 and α-smooth muscle actin expression were significantly higher than in HC-MSCs. In both SSc- and HC-MSCs, PDGF-BB induced increased expression of Ki-67 when compared with untreated cells but was unable to modulate CD248 levels. After CD248 silencing, both TGF-β and PDGF-BB signaling were inhibited in SSc-MSCs. Conclusions: CD248 overexpression may play an important role in the fibrotic process by modulating the molecular target, leading to perivascular cells differentiation toward myofibroblasts and interfering with its expression, and thus might open a new therapeutic strategy to inhibit myofibroblast generation during SSc

The Brera Multi-scale Wavelet (BMW) ROSAT HRI source catalog. II: application to the HRI and first results

The wavelet detection algorithm (WDA) described in the accompanying paper by Lazzati et al. is made suited for a fast and efficient analysis of images taken with the High Resolution Imager (HRI) instrument on board the ROSAT satellite. An extensive testing is carried out on the detection pipeline: HRI fields with different exposure times are simulated and analysed in the same fashion as the real data. Positions are recovered with few arcsecond errors, whereas fluxes are within a factor of two from their input values in more than 90% of the cases in the deepest images. At variance with the ``sliding-box'' detection algorithms, the WDA provides also a reliable description of the source extension, allowing for a complete search of e.g. supernova remnant or cluster of galaxies in the HRI fields. A completeness analysis on simulated fields shows that for the deepest exposures considered (~120 ks) a limiting flux of \~3x10^{-15} erg/cm2/s can be reached over the entire field of view. We test the algorithm on real HRI fields selected for their crowding and/or presence of extended or bright sources (e.g. cluster of galaxies and of stars, supernova remnants). We show that our algorithm compares favorably with other X-ray detection algorithms such as XIMAGE and EXSAS. A complete catalog will result from our analysis: it will consist of the Brera Multi-scale Wavelet Bright Source Catalog (BMW-BSC) with sources detected with a significance >4.5 sigma and of the Faint Source Catalog (BMW-FSC) with sources at >3.5 sigma. A conservative estimate based on the extragalactic log(N)-log(S) indicates that at least 16000 sources will be revealed in the complete analysis of the whole HRI dataset.Comment: 6 pages, 11 PostScript figures, 1 gif figure, ApJ in pres

Influence of short-term storage on electrophoretic profile of bovine serum proteins

Storage represents a basic step of the pre-analytical phase for the accuracy of screening tests both in human and veterinary diagnostic laboratories. This study aimed to evaluate the effect of short-term storage on electrophoretic profile of serum proteins in dairy cows. Blood was collected from 18 clinically healthy cows and serum was divided into four aliquots. The first aliquot was immediately analysed (time 0). The second and the third aliquots were stored at +4°C for 24 and 48 h, respectively. The fourth aliquot was frozen at −20° C for 48 hours. A statistical analysis was performed on the obtained data. Albumin and α-globulins relative concentration and A/G ratio showed significant changes after 48 h storage. The results obtained in the current study showed that short-term storage influenced electrophoretic pattern of bovine serum proteins and the A/G ratio. Therefore, the refrigeration of bovine sera for 24 h at the most should be suggested to practitioners in order to obtain reliable serum protein..

The Brera Multi-scale Wavelet Chandra Survey. I. Serendipitous source catalogue

We present the BMW-Chandra source catalogue drawn from essentially all Chandra ACIS-I pointed observations with an exposure time in excess of 10ks public as of March 2003 (136 observations). Using the wavelet detection algorithm developed by Lazzati et al. (1999) and Campana et al. (1999), which can characterise both point-like and extended sources, we identified 21325 sources. Among them, 16758 are serendipitous, i.e. not associated with the targets of the pointings, and do not require a non-automated analysis. This makes our catalogue the largest compilation of Chandra sources to date. The 0.5--10 keV absorption corrected fluxes of these sources range from ~3E-16 to 9E-12 erg cm^-2 s^-1 with a median of 7E-15 erg cm^-2 s^-1. The catalogue consists of count rates and relative errors in three energy bands (total, 0.5-7keV; soft, 0.5-2keV; and hard, 2-7keV), and source positions relative to the highest signal-to-noise detection among the three bands. The wavelet algorithm also provides an estimate of the extension of the source. We include information drawn from the headers of the original files, as well, and extracted source counts in four additional energy bands, SB1 (0.5-1keV), SB2 (1-2keV), HB1 (2-4keV), and HB2 (4-7keV). We computed the sky coverage for the full catalogue and for a subset at high Galactic latitude (|b|> 20deg). The complete catalogue provides a sky coverage in the soft band (0.5-2keV, S/N =3) of ~8 deg^2 at a limiting flux of 1E-13 erg cm^-2 s^-1, and ~2 deg^2 at a limiting flux of ~1E-15 erg cm^-2 s^-1.Comment: Accepted by A&A, Higher res. Figs 4 and 5 at http://www.ifc.inaf.it/~romano/BMC/Docs/aapaper/9601f4.eps http://www.ifc.inaf.it/~romano/BMC/Docs/aapaper/9601f5.eps, Catalog Web pages: http://www.brera.inaf.it/BMC/bmc_home.html http://www.ifc.inaf.it/~romano/BMC/bmc_home.html (Mirror

The Brera Multi-scale Wavelet ROSAT HRI source catalogue (BMW-HRI)

We present the Brera Multi-scale Wavelet ROSAT HRI source catalogue (BMW-HRI) derived from all ROSAT HRI pointed observations with exposure time longer than 100 s available in the ROSAT public archives. The data were analyzed automatically using a wavelet detection algorithm suited to the detection and characterization of both point-like and extended sources. This algorithm is able to detect and disentangle sources in very crowded fields and/or in presence of extended or bright sources. Images have been also visually inspected after the analysis to ensure verification. The final catalogue, derived from 4,303 observations, consists of 29,089 sources detected with a detection probability of greater or equal 4.2 sigma. For each source, the primary catalogue entries provide name, position, count rate, flux and extension along with the relative errors. In addition, results of cross-correlations with existing catalogues at different wavelengths (FIRST, IRAS, 2MASS and GSC2) are also reported. All these information are available on the web via the DIANA Interface. As an external check, we compared our catalogue with the previously available ROSHRICAT catalogue (both in its short and long versions) and we were able to recover, for the short version, \~90% of the entries. We computed the sky coverage of the entire HRI data set by means of simulations. The complete BMW-HRI catalogue provides a sky coverage of 732 square degrees down to a limiting flux of ~1E-12 erg/s/cm2 and of 10 square degrees down to ~1E-14 erg/s/cm2. We were able to compute the cosmological log(N)-log(S) distribution down to a flux of about 1.2x1E-14 erg/s/cm2