Web Application for Monitoring the Conversion of Water to Dry Matter for Development in Different Soybean Genotypes

El presente trabajo realiza la implementación de un sistema de riego por goteo utilizando sensores NPK, el cual es adecuado para medir la temperatura, humedad, conductividad, el PH del suelo, asimismo detecta el contenido de nitrógeno, fosforo y potasio en el suelo. Este sistema permitirá monitorear el uso adecuado del agua para aplicarlo en un cultivo de soya de cuatro genotipos basado en materia seca; con estos datos obtenidos de los sensores se mostrará en una aplicación web para estar analizando en tiempo real durante las pruebas de campo.This work implements a drip irrigation system using NPK sensors, which is suitable for measuring temperature, humidity, conductivity, and soil PH, and also detects the nitrogen, phosphorus, and potassium content in the soil. This system will allow monitoring the appropriate use of water to apply it to a soybean crop of four genotypes based on dry matter; With this data obtained from the sensors it will be displayed in a web application to be analyzed in real time during field tests

Genomic epidemiology of NDM-1-encoding plasmids in latin American clinical isolates reveals insights into the evolution of multidrug resistance

Bacteria that produce the broad-spectrum Carbapenem antibiotic NewDelhi Metallo-b-lactamase (NDM) place a burden on health care systems worldwide, due to the limited treatment options for infections caused by them and the rapid global spread of this antibiotic resistancemechanism.Although it is believed that theassociated resistancegenebla NDM-1 originated inAcinetobacter spp., the role of Enterobacteriaceae in its dissemination remains unclear. In this study, we usedwhole genome sequencing to investigate the dissemination dynamics of blaNDM-1-positive plasmids in a set of 21 clinical NDM-1-positive isolates from Colombia and Mexico (Providencia rettgeri, Klebsiella pneumoniae, and Acinetobacter baumannii) aswell as six representative NDM-1-positive Escherichia coli transconjugants. Additionally, the plasmids from three representative P. rettgeri isolates were sequenced by PacBio sequencing and finished. Our results demonstrate the presence of previously reported plasmids from K. pneumoniae and A. baumannii in different genetic backgrounds and geographically distant locations in Colombia. Three new previously unclassified plasmids were also identified in P. rettgeri from Colombia and Mexico, plus an interesting genetic link between NDM-1-positive P. rettgeri from distant geographic locations (Canada, Mexico, Colombia, and Israel) without any reported epidemiological links was discovered. Finally, we detected a relationship between plasmids present in P. rettgeri and plasmids from A. baumannii and K. pneumoniae. Overall, our findings suggest a Russian dollmodel for the dissemination of blaNDM-1 in LatinAmerica,with P. rettgeri playing a central role in this process, andrevealnewinsights into the evolution and disseminationof plasmids carrying such antibiotic resistance genes

Recursos didácticos para la docencia y mejora de la empleabilidad: aplicación a la asignatura Edición musical del Máster en Música Española e Hispanoamericana (II)

El objetivo principal es crear recursos didácticos para la mejora de la enseñanza de la edición de música, una de las actividades profesionales más habituales que realizan los músicos y los musicólogos pero apenas existen materiales ni recursos didácticos para su enseñanza

Role of age and comorbidities in mortality of patients with infective endocarditis

[Purpose]: The aim of this study was to analyse the characteristics of patients with IE in three groups of age and to assess the ability of age and the Charlson Comorbidity Index (CCI) to predict mortality. [Methods]: Prospective cohort study of all patients with IE included in the GAMES Spanish database between 2008 and 2015.Patients were stratified into three age groups:<65 years,65 to 80 years,and ≥ 80 years.The area under the receiver-operating characteristic (AUROC) curve was calculated to quantify the diagnostic accuracy of the CCI to predict mortality risk. [Results]: A total of 3120 patients with IE (1327 < 65 years;1291 65-80 years;502 ≥ 80 years) were enrolled.Fever and heart failure were the most common presentations of IE, with no differences among age groups.Patients ≥80 years who underwent surgery were significantly lower compared with other age groups (14.3%,65 years; 20.5%,65-79 years; 31.3%,≥80 years). In-hospital mortality was lower in the <65-year group (20.3%,<65 years;30.1%,65-79 years;34.7%,≥80 years;p < 0.001) as well as 1-year mortality (3.2%, <65 years; 5.5%, 65-80 years;7.6%,≥80 years; p = 0.003).Independent predictors of mortality were age ≥ 80 years (hazard ratio [HR]:2.78;95% confidence interval [CI]:2.32–3.34), CCI ≥ 3 (HR:1.62; 95% CI:1.39–1.88),and non-performed surgery (HR:1.64;95% CI:11.16–1.58).When the three age groups were compared,the AUROC curve for CCI was significantly larger for patients aged <65 years(p < 0.001) for both in-hospital and 1-year mortality. [Conclusion]: There were no differences in the clinical presentation of IE between the groups. Age ≥ 80 years, high comorbidity (measured by CCI),and non-performance of surgery were independent predictors of mortality in patients with IE.CCI could help to identify those patients with IE and surgical indication who present a lower risk of in-hospital and 1-year mortality after surgery, especially in the <65-year group

Ciencia Odontológica 2.0

Libro que muestra avances de la Investigación Odontológica en MéxicoEs para los integrantes de la Red de Investigación en Estomatología (RIE) una enorme alegría presentar el segundo de una serie de 6 libros sobre casos clínicos, revisiones de la literatura e investigaciones. La RIE está integrada por cuerpos académicos de la UAEH, UAEM, UAC y UdeG

Synthèse d'oligonucléotides renfermant des lésions inter-thyminiques

Les lésions provoquées entre deux thymines dans l'ADN, sous rayonnement UV ou ionisant, peuvent être à l'origine de la carcinogenèse. Ces adduits suscitent un grand intérêt pour les études mécanistiques de formation et de réparation par l'organisme. Pour cela, il est nécessaire de disposer d'oligonucléotides, de séquence choisie renfermant la lésion, dont la meilleure voie d'accès reste la synthèse chimique.La première partie de cette thèse concerne la synthèse des lésions photochimiques majeures de l'ADN sous irradiation UV, les adduits cyclobutanique et pyrimidine(6-4)pyrimidone et la mise au point d'une nouvelle voie d'incorporation de ces adduits dans les ODNs. Nous avons décrit la synthèse d'un nouveau phosphoramidite de l'adduit cis-syn CPD de thymine ainsi que son incorporation dans des ODNs par synthèse automatique sur support solide. L'originalité de cette synthèse repose sur l'emploi du groupement protecteur lévulinyle qui est stable dans les conditions de photolyse et compatible avec la synthèse sur support solide. Pour obtenir un phosphoramidite renfermant l'adduit (6-4)PP, nous avons étudié l'influence des groupements protecteurs d'un dinucléotide de thymine sur sa photoréactivité.La deuxième partie est consacrée à la synthèse d'adduits 6-(a-thyminyl)-5,6-dihydrothymine en vue de la préparation d'ODNs. Ils ont été synthétisés en utilisant la photoréactivité de la 4-thiothymine. L'évaluation de leur stabilité a permis d'envisager la synthèse des phosphoramidites correpondants. En série protégée, la synthèse est compatible avec la protection du phosphate internucléotidique. L'emploi du groupement protecteur adapté en 5' devrait conduire au phosphoramidite.UV or ionizing radiations can induce lesions between adjacent thymines in DNA that can lead to mutation and carcinogenesis. These lesions are the subject of intensive studies in the field of their formation and repair mechanism. To better understand their effects, it is necessary to work on oligonucleotides of determined sequences incorporating the lesions and the best way to obtain them remains the chemical synthesis.The first part of this work concerns the synthesis of the major UV induced DNA lesions, the cyclobutane and the pyrimidine(6-4)pyrimidone adducts, and a new approach which improves the insertion of these adducts into ODNs. The synthesis of a new phosphoramidite building block of the thymine cis-syn CPD and its incorporation in ODNs by automated solid support synthesis is described. The originality of this work is based on the use of the levulinyl protecting group which is stable in the photolysis step and compatible with the conditions of solid phase synthesis. In view of obtaining the phosphoramidite building block of the (6-4)PP adduct, we have studied the influence of protecting groups on the photochemical reactivity of a thymine dinucleotide.The second part concerns the synthesis of 6-(?-thyminyl)-5,6-dihydrothymine adducts to prepare ODNs. These adducts were synthesized by taking advantage of the photochemical reactivity of a 4-thiothymine derivative. The tests of stability make their incorporation into ODNs possible. The internucleotide phosphate protecting group is compatible with the conditions employed for the synthesis of these adducts. The use of an adequate 5'-protecting group should afford the corresponding phosphoramidite building block.ORSAY-PARIS 11-BU Sciences (914712101) / SudocSudocFranceF

Recursos didácticos para la docencia y mejora de la empleabilidad: aplicación a la asignatura Edición Musical del Máster en Música Española e Hispanoamericana

El objetivo principal es crear recursos didácticos en abierto para la docencia de la edición crítica de música, una materia que cuenta con escasos materiales didácticos en castellano y que es fundamental para la empleabilidad de los alumnos