Fungal solid state fermentation on agro-industrial wastes for acid wastewater decolourization in a continuous flow packed-bed bioreactor

This study was aimed at developing a process of solid state fermentation (SSF) with the fungi Pleurotus ostreatus and Trametes versicolor on apple processing residues for wastewater decolorization. Both fungi were able to colonize apple residues without any addition of nutrients, material support or water. P. ostreatus produced the highest levels of laccases (up to 9 U g-1 of dry matter) and xylanases (up to 80 U g-1 of dry matter). A repeated batch decolorization experiment was set up with apple residues colonized by P. ostreatus, achieving 50% decolorization and 100% detoxification after 24 h, and, adding fresh wastewater every 24 h, a constant decolorization of 50% was measured for at least 1 month. A continuous decolorization experiment was set up by a packed-bed reactor based on colonized apple residues achieving a performance of 100 mg dye L-1 day-1 at a retention time of 50

Strikingly higher frequency in centenarians and twins of mtDNA mutation causing remodeling of replication origin in leukocytes

The presence of a genetic component in longevity is well known. Here, the association of a mtDNA mutation with a prolonged life span in humans was investigated. Large-scale screening of the mtDNA main control region in leukocytes from subjects of an Italian population revealed a homoplasmic C150T transition near an origin of heavy mtDNA-strand synthesis in approximate to 17% of 52 subjects 99-106 years old, but, in contrast, in only 3.4% of 117 younger individuals (P = 0.0035). Evidence was obtained for the contribution of somatic events, under probable nuclear genetic control, to the striking selective accumulation of the mutation in centenarians. In another study, among leukocyte mtDNA samples from 20 monozygotic and 18 dizygotic twins, 60-75 years old, 30% (P = 0.0007) and 22% (P = 0.011), respectively, of the individuals involved exhibited the homoplasmic C150T mutation. In a different system, i.e., in five human fibroblast longitudinal studies, convincing evidence for the aging-related somatic expansion of the C150T mutation, up to homoplasmy, was obtained. Most significantly, 5' end analysis of nascent heavy mtDNA strands consistently revealed a new replication origin at position 149, substituting for that at 151, only in C150T mutation carrying samples of fibroblasts or immortalized lymphocytes. Considering the aging-related health risks that the centenarians have survived and the developmental risks of twin gestations, it is proposed that selection for a remodeled replication origin, inherited or somatically acquired, provides a survival advantage and underlies the observed high incidence of the C150T mutation in centenarians and twins

A Stochastic Model to Evaluate Pricing Distortions in Indemnity Insurance Methods for MTPL Insurance

Direct compensation or the direct reimbursement scheme is an indemnity insurance method that many European and American countries use to manage motor liability claims in which the driver that suffers an accident is paid by his/her insurance company that possibly later receives a flat-rate reimbursement (known as forfeit). Using non-life actuarial methodologies, this article analyses the distortion effects due to the direct compensation mechanisms and the effects of different forfeit reimbursement systems on policyholder tariffs in the management of motor liability claims involving vehicles in two different sectors, i.e. automobile and motorcycle. We empirically analyse and formalize the distortion effects resulting from the mechanism that different direct reimbursement systems produce, and explore the correlation between increasing tariffs for motorcycle policyholders and decreasing tariffs for other vehicle policyholders. We propose some alternative methods to overcome these distortion effects, evaluating their pricing impact through a stochastic model applied to a case study

Bioreactor and bioprocess design issues in enzymatic hydrolysis of lignocellulosic biomass

publishedVersio

Bioremediation: an overview on current practices, advances, and new perspectives in environmental pollution treatment

[Excerpt] Environmental pollution generated the need to search for new environmentally friendly, low-cost, and more efficient environmental clean-up techniques for its removal or reduction. Bioremediation, a branch of environmental biotechnology, is nowadays considered as one of the most promising alternatives. This technology uses the amazing ability of microorganisms or plants to accumulate, detoxify, degrade, or remove environmental contaminants. Bioremediation provides the transformation and/or even removal of organic and inorganic pollutants, even when they are present at low concentration. Continuous efforts are still made to understand the mechanisms by which microorganisms and plants remove or transform environmental pollutants. Thus, the purpose of this special issue was to explore different visions on bioremediation, while addressing recent advances and new ideas in the perspective of efficient process scale-up in view of application at larger scales. Authors’ contributions cover various topics with a range of papers including original research and review articles spanning studies in remediation of different environments which outline new findings in the biotechnology field. This special issue contains five papers including one review article and four original research articles. A brief description of these five manuscripts is detailed below. [...]We would like to extend our gratitude to all the authors who submitted their work for consideration in our special issue and to reviewers for their critical feedback. Contributions of Raluca Maria Hlihor and Maria Gavrilescu to this special issue were supported by a grant of the Romanian National Authority for Scientific Research,CNCS-UEFISCDI (Project no. PN-III-P4-ID-PCE-2016-0683, Contract no. 65/2017). Teresa Tavares’ contribution is supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the research project PTDC/AAG-TEC/5269/2014, the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of NORTE 2020 (Programa Operacional Regional do Norte).info:eu-repo/semantics/publishedVersio

The prognostic effects of circulating myeloid-derived suppressor cells in non-small cell lung cancer: systematic review and meta-analysis

Immunotherapy is the main standard treatment for non-small cell lung cancer (NSCLC) patients. Immune suppressive cells in tumor microenvironment can counteract its efficacy. Myeloid-derived suppressor cells (MDSCs) include two major subsets: polymorphonuclear (PMN-MDSCs) and monocytic (M-MDSCs). Many studies explored the prognostic impact of these cell populations in NSCLC patients. The aim of this systematic review is to select studies for a meta-analysis, which compares prognosis between patients with high vs low circulating MDSC levels. We collected hazard ratios (HRs) and relative 95% confidence intervals (CIs) in terms of progression-free survival (PFS) or recurrence-free survival (RFS), and overall survival (OS). Among 139 studies retrieved from literature search, 14 eligible studies (905 NSCLC patients) met inclusion criteria. Low circulating MDSC levels favor a better PFS/RFS (HR = 1.84; 95% CI = 1.28-2.65) and OS (HR = 1.78; 95% CI = 1.29-2.46). The subgroup analysis based on MDSC subtypes (total-, PMN-, and M-MDSCs) obtained a statistical significance only for M-MDSCs, both in terms of PFS/RFS (HR = 2.67; 95% CI = 2.04-3.50) and OS (HR = 2.10; 95% CI = 1.61-2.75). NSCLC patients bearing high M-MDSC levels in peripheral blood experience a worse prognosis than those with low levels, both in terms of PFS/RFS and OS. This finding suggests that detecting and targeting this MDSC subset could help to improve NSCLC treatment efficacy

Klessydra-T: Designing Vector Coprocessors for Multi-Threaded Edge-Computing Cores

Computation intensive kernels, such as convolutions, matrix multiplication and Fourier transform, are fundamental to edge-computing AI, signal processing and cryptographic applications. Interleaved-Multi-Threading (IMT) processor cores are interesting to pursue energy efficiency and low hardware cost for edge-computing, yet they need hardware acceleration schemes to run heavy computational workloads. Following a vector approach to accelerate computations, this study explores possible alternatives to implement vector coprocessing units in RISC-V cores, showing the synergy between IMT and data-level parallelism in the target workloads.Comment: Final revision accepted for publication on IEEE Micro Journa

CFD Simulation of Binary Fluidized Mixtures: Effects of Restitution Coefficient and Spatial Discretization Methods

The work focuses on the CFD simulation of fluidized bidisperse solid particles with same density and different size. We successfully predicted the minimum superficial gas velocity required to steadily fluidize the particles by employing a second-order upwind spatial discretization method and a non-ideal value of the restitution coefficient

transcriptional response of human umbilical vein endothelial cells to low doses of ionizing radiation

We used cDNA microarray hybridization technology to monitor the transcriptional response of Human Umbilical Vein Endothelial (HUVEC) cells to x-rays doses ranging from 2 to 200 cGy. An early time window from irradiation (4h) was selected in order to minimize the effects of the cell cycle blockage eventually induced at high doses of irradiation. Three different gene-clustering algorithms have been used to group the 4134 monitored ORF based on their transcriptional response in function of the irradiation dose. The results show that while few genes exhibit a typical dose-dependent modulation with a variable threshold, most of them have a different modulation pattern, peaking at the two intermediate doses. Strikingly even the lowest dose used (2 cGy) seems to be very effective in transcriptional modulation. These results confirm the physiological relevance of sublethal-dose exposures of endothelial cells and strengthens the hypothesis that alternative dose-specific pathways of radioadaptive response exist in the mammalian cells. 111 genes were found to be modulated at all doses of irradiation. These genes were functionally classified by cellular process or by molecular function. Genes involved in coagulation and peroxidase activity and structural constituent of ribosomes were over-represented among the up-regulated genes as compared with their expected statistical occurrence. Three genes coding for regulatory kinase activities (CDK6; PRCKB1 and TIE) are found down-regulated at all doses of irradiation