The development of dairying in Europe: potential evidence from food residues on ceramics

Providing evidence of dairying is crucial to the understanding of the development and intensification of Neolithic farming practices in Europe, beyond the early stages of domestication. Until recently, research in this field had been limited to traditional archaeological methods, such as the study of pottery styles, faunal remains and specialised material artefacts. Although suggestive, these methods are unable to provide direct evidence of dairying. Advances in biomolecular methods now allow the identification of remnants of dairy products on ceramic vessels and the application of these methods to Neolithic ceramic assemblages across Europe is underway. There is no doubt that these new methods offer much scope for investigating hypotheses such as the ‘secondary products revolution’, but there are limitations. The cost of analyses prohibits indiscriminate sampling and differential survival is likely to prevent direct comparison of samples from different sites. Only by incorporating these techniques within the wider frameworks of archaeological research may theories be properly tested. Approaches to achieve this goal are discussed.Iskanje dokazov za mlekarstvo je ključnega pomena za razumevanje razvoja in intenzviranja kmetovanja v Evropi po začetnih stopnjah udomačitve. Do nedavnega so bile raziskave na tem področju omejene na tradicionalne arheološke metode, kot je raziskovanje stilov keramike, živalskih ostankov in specialnih predmetov. Čeprav mnogo povedo, pa te metode niso mogle zagotoviti neposrednih dokazov za mlekarstvo. Razvoj biomolekularnih metod danes omogoča določevanje ostankov mlečnih produktov na keramičnih posodah. Raziskave neolitske keramike po Evropi že potekajo. Nedvomno lahko z novimi metodami natančneje raziščemo hipoteze, kot je na primer ‘sekundarna revolucija proizvodov’, toda tudi tu so omejitve. Cena analiz onemogoča sistematično vzorčenje, diferencirani načini preživljanja pa bodo verjetno preprečili neposredno primerjavo vzorcev z različnih najdišč. Le če bomo vključili nove tehnike v širši okvir arheoloških raziskav, bomo lahko teorije primerno preverili. V članku razpravljamo o načinih za dosego tega cilja

Investigating the function of prehistoric stone bowls and griddle stones in the Aleutian Islands by lipid residue analysis

The earliest durable cooking technologies found in Alaska are stone bowls and griddle stones recovered from the Aleutian Islands. This article aims to identify the function of these artefacts. Molecular and chemical analyses of carbonised residues found on their surfaces confirm that these artefacts were used to process marine resources. Both artefacts have high lipid content and C:N ratios, suggesting they were used to process oily substances. Stable isotope results of individual lipids suggest that they were used to process different sets of resources within the aquatic spectrum as griddle stones have slightly more 13C-depleted lipids than stone bowls, possibly indicating more variable use. Integration of these results with archaeological and ethnographic data leads us to infer that griddle stones were used for cooking a diversity of aquatic resources, possibly with the addition of plant foods, whereas stone bowls were specifically used to render marine mammal fats. We further hypothesize that a sudden peak in stone bowl frequencies at 4000–3000 cal yr BP was connected to a Neoglacial cold spell bringing sea ice conditions to the Aleutian Islands. This may have led to new subsistence strategies in which the rendering of marine mammal fats played a central role

Resource Processing, Early Pottery and the Emergence of Kitoi Culture in Cis-Baikal:Insights from Lipid Residue Analysis of an Early Neolithic Ceramic Assemblage from the Gorelyi Les Habitation Site, Eastern Siberia

In the early Holocene, Mesolithic hunter-gatherer communities inhabiting the Cis-Baikal region of Eastern Siberia were participating in a series of important cultural changes. These included the establishment of large cemeteries in the Angara Valley and on the Southwest shores of Lake Baikal, culminating in the formation of the distinctive Early Neolithic Kitoi cultural pattern ca. 7560 cal. BP. Around the same time, the appearance of clay pots in a few Kitoi graves and at some contemporary habitation sites marks the formal transition to the Early Neolithic, which is defined in Russian archaeology by the emergence of pottery (and not the transition to farming). Little is known about how this early pottery was used, and why it was first adopted into the region. This pilot-study presents lipid-residue analysis of a selection of sherds from the oldest and relatively well-dated pottery assemblage in the Cis-Baikal region, which was recovered from the Gorelyi Les habitation site. The results indicate that the pots had been used to process a broad spectrum of food resources, including ruminants, fish and plants, and possibly resin and other by-products derived from pine trees, suggesting that the vessels were being used as general-purpose cooking containers. We conclude that there is scope for a much larger-scale investigation of diversity and change in prehistoric pottery use in Cis-Baikal, and that this research would improve current understandings of the diet, health and subsistence strategies of the Kitoi and other prehistoric populations

Lipid residue analysis on Swifterbant pottery (c. 5000-3800 cal BC) in the Lower Rhine-Meuse area (the Netherlands) and its implications for human-animal interactions in relation to the Neolithisation process

This paper focuses on the functional analysis of Swifterbant pottery (c. 5000–3800 cal BC) in the Lower Rhine-Meuse area (the Netherlands). It examines pottery use across the transition to agriculture and aims to assess temporal changes in human-animal relations during the 5th millennium BC in the Lower Rhine-Meuse area through lipid residue analysis. We conducted lipid residue analysis of 49 samples from four Swifterbant sites: Hardinxveld-Giessendam Polderweg, Hardinxveld-Giessendam De Bruin, Brandwijk-het Kerkhof, and Hazendonk. A combined approach using both GC-MS and GC-C-IRMS of residues absorbed into the ceramic was employed to identify their context. Their context was then compared to published faunal datasets to present the relative abundance of taxa detected in the lipid residues. Evidence of processing freshwater fish was found in all sites, presenting that it was a continuous and primary function of Swifterbant pottery in the Lower Rhine-Meuse area starting from its first appearance at c. 5000 cal BC till the end of 5th millennium BC regardless of vessel form, size, decoration or temper. The results of our analysis also present temporal changes in the exploitation of food resources from the early to the late 5th millennium BC. From the mid 5th millennium BC onwards, vessels were also used to process different ranges of foodstuffs such as terrestrial resources and dairy products. The identification of dairy residue is the first direct evidence so far from Swifterbant pottery. We tentatively explain these results as an indication of presence of different culinary practices that had developed through the 5th millennium in the Lower Rhine-Meuse area and that the use of Swifterbant pottery is a direct reflection of changing cultural preferences on food preparation and consumption

Production and function of Neolithic black-painted pottery from Schela Cladovei (Iron Gates, Romania)

This paper presents for the first time the results of a combination of petrographic, geochemical and organic residue analyses of early Neolithic ceramics from the Iron Gates region of the Danube basin. Eleven early Neolithic potsherds from Schela Cladovei (Romania) were analysed in detail. The results of the petrographic analysis show that the ceramics were made with the same recipe that was used by Starčevo-Körös-Criș potters elsewhere in southeastern Europe. The SEM-EDX analysis shows one of the earliest uses of Mn-rich black pigments to decorate Neolithic European ceramics. Organic residue analyses detected dairy, non-ruminant and ruminant adipose fats. No evidence of aquatic resources was detected. In summary, the early Neolithic potters at the Iron Gates, although able to make coarse and more sophisticated painted ceramics, did not make specific vessels for a specific use

Intraoperative electrocochleographic characteristics of auditory neuropathy spectrum disorder in cochlear implant subjects

Auditory neuropathy spectrum disorder (ANSD) is characterized by an apparent discrepancy between measures of cochlear and neural function based on auditory brainstem response (ABR) testing. Clinical indicators of ANSD are a present cochlear microphonic (CM) with small or absent wave V. Many identified ANSD patients have speech impairment severe enough that cochlear implantation (CI) is indicated. To better understand the cochleae identified with ANSD that lead to a CI, we performed intraoperative round window electrocochleography (ECochG) to tone bursts in children (n = 167) and adults (n = 163). Magnitudes of the responses to tones of different frequencies were summed to measure the “total response” (ECochG-TR), a metric often dominated by hair cell activity, and auditory nerve activity was estimated visually from the compound action potential (CAP) and auditory nerve neurophonic (ANN) as a ranked “Nerve Score”. Subjects identified as ANSD (45 ears in children, 3 in adults) had higher values of ECochG-TR than adult and pediatric subjects also receiving CIs not identified as ANSD. However, nerve scores of the ANSD group were similar to the other cohorts, although dominated by the ANN to low frequencies more than in the non-ANSD groups. To high frequencies, the common morphology of ANSD cases was a large CM and summating potential, and small or absent CAP. Common morphologies in other groups were either only a CM, or a combination of CM and CAP. These results indicate that responses to high frequencies, derived primarily from hair cells, are the main source of the CM used to evaluate ANSD in the clinical setting. However, the clinical tests do not capture the wide range of neural activity seen to low frequency sounds

Vertical integration and firm boundaries : the evidence

Since Ronald H. Coase's (1937) seminal paper, a rich set of theories has been developed that deal with firm boundaries in vertical or input–output structures. In the last twenty-five years, empirical evidence that can shed light on those theories also has been accumulating. We review the findings of empirical studies that have addressed two main interrelated questions: First, what types of transactions are best brought within the firm and, second, what are the consequences of vertical integration decisions for economic outcomes such as prices, quantities, investment, and profits. Throughout, we highlight areas of potential cross-fertilization and promising areas for future work

The role of salmon fishing in the adoption of pottery technology in subarctic Alaska

Ceramic technology makes an abrupt appearance in the New World Arctic at circa 2800 cal BP. While there is general consensus that the ultimate source of these Alaskan pottery traditions lay in continental NE Asia, the motivations for the adoption of pottery in Alaska have remained unclear. Through organic residue analysis we investigated the function of Norton pottery in Southwest Alaska, and the extent to which its function changed in later periods under the increasing northern influence of Thule culture in the region (from ca. 1000 cal BP). Our results show clear evidence of aquatic resource processing in all pottery vessels. Regional variability due to environmental and ecological differences are apparent in the pottery. The majority of Norton pottery was from inland riverine locations and the function of this early pottery was to process anadromous fish, with only limited evidence of other resources. After 1000 cal BP more sites appear on the coast, and while pottery technology changes dramatically at this time, this is not as clear in pottery function which remains aimed at local abundant aquatic resources. We hypothesize that pottery was adopted into Alaska as part of a riverine adaptation and suggest that targeted human exploitation of large riverine systems may have facilitated its expansion into Southwest Alaska. Furthermore, we suggest that this pattern might extend back into Siberia where Alaskan pottery originates

Electrochemical and thermal detection of allergenic substance lysozyme with molecularly imprinted nanoparticles

Lysozyme (LYZ) is a small cationic protein which is widely used for medical treatment and in the food industry to act as an anti-bacterial agent; however, it can trigger allergic reactions. In this study, high-affinity molecularly imprinted nanoparticles (nanoMIPs) were synthesized for LYZ using a solid-phase approach. The produced nanoMIPs were electrografted to screen-printed electrodes (SPEs), disposable electrodes with high commercial potential, to enable electrochemical and thermal sensing. Electrochemical impedance spectroscopy (EIS) facilitated fast measurement (5–10 min) and is able to determine trace levels of LYZ (pM) and can discriminate between LYZ and structurally similar proteins (bovine serum albumin, troponin-I). In tandem, thermal analysis was conducted with the heat transfer method (HTM), which is based on monitoring the heat transfer resistance at the solid–liquid interface of the functionalized SPE. HTM as detection technique guaranteed trace-level (fM) detection of LYZ but needed longer analysis time compared to EIS measurement (30 min vs 5–10 min). Considering the versatility of the nanoMIPs which can be adapted to virtually any target of interest, these low-cost point-of-care sensors hold great potential to improve food safety