Influencia del soporte en catalizadores FePt para la reacción de CO-PROX

En el presente trabajo se prepararon catalizadores de 1% Pt/γ-Al2O3 y 1% Pt/SiO2 sobre los que se depositaron diferentes cargas de Fe. Estos fueron evaluados en la reacción de oxidación preferencial de CO (CO-PROX) entre 60 y 270°C con relación estequiométrica O2/CO. Los catalizadores fueron estudiados por reducción a temperatura programada (TPR), revelando que existe una fuerte interacción entre los metales. Los resultados de los ensayos de actividad muestran que el agregado de Fe en los catalizadores Pt/Al2O3 mejora la conversión de CO y que la adición de Fe en el catalizador Pt/SiO2 conduce a conversiones similares a las del catalizador 1% Pt/SiO2, aunque a menores temperaturas. Cada serie presenta una carga óptima de hierro con la cual se logró la mayor conversión de CO.Publicado en Terceras Jornadas de Investigación, Transferencia y Extensión. La Plata : Universidad Nacional de La Plata, 2015.Facultad de Ingenierí

Influencia del soporte en catalizadores FePt para la reacción de CO-PROX

En el presente trabajo se prepararon catalizadores de 1% Pt/γ-Al2O3 y 1% Pt/SiO2 sobre los que se depositaron diferentes cargas de Fe. Estos fueron evaluados en la reacción de oxidación preferencial de CO (CO-PROX) entre 60 y 270°C con relación estequiométrica O2/CO. Los catalizadores fueron estudiados por reducción a temperatura programada (TPR), revelando que existe una fuerte interacción entre los metales. Los resultados de los ensayos de actividad muestran que el agregado de Fe en los catalizadores Pt/Al2O3 mejora la conversión de CO y que la adición de Fe en el catalizador Pt/SiO2 conduce a conversiones similares a las del catalizador 1% Pt/SiO2, aunque a menores temperaturas. Cada serie presenta una carga óptima de hierro con la cual se logró la mayor conversión de CO.Publicado en Terceras Jornadas de Investigación, Transferencia y Extensión. La Plata : Universidad Nacional de La Plata, 2015.Facultad de Ingenierí

Influencia del soporte en catalizadores FePt para la reacción de CO-PROX

En el presente trabajo se prepararon catalizadores de 1% Pt/γ-Al2O3 y 1% Pt/SiO2 sobre los que se depositaron diferentes cargas de Fe. Estos fueron evaluados en la reacción de oxidación preferencial de CO (CO-PROX) entre 60 y 270°C con relación estequiométrica O2/CO. Los catalizadores fueron estudiados por reducción a temperatura programada (TPR), revelando que existe una fuerte interacción entre los metales. Los resultados de los ensayos de actividad muestran que el agregado de Fe en los catalizadores Pt/Al2O3 mejora la conversión de CO y que la adición de Fe en el catalizador Pt/SiO2 conduce a conversiones similares a las del catalizador 1% Pt/SiO2, aunque a menores temperaturas. Cada serie presenta una carga óptima de hierro con la cual se logró la mayor conversión de CO.Publicado en Terceras Jornadas de Investigación, Transferencia y Extensión. La Plata : Universidad Nacional de La Plata, 2015.Facultad de Ingenierí

X-ray, UV, and optical observations of the accretion disk and boundary layer in the symbiotic star RT Crucis

Compared to mass transfer in cataclysmic variables, the nature of accretion in symbiotic binaries in which red giants transfer material to white dwarfs (WDs) has been difficult to uncover. The accretion flows in a symbiotic binary are most clearly observable, however, when there is no quasi-steady shell burning on the WD to hide them. RT Cru is the prototype of such non-burning symbiotics, with its hard (δ-type) X-ray emission providing a view of its innermost accretion structures. In the past 20 yr, RT Cru has experienced two similar optical brightening events, separated by ∼ 4000 days and with amplitudes of ΔV ∼ 1.5 mag. After Swift became operative, the Burst Alert Telescope (BAT) detector revealed a hard X-ray brightening event almost in coincidence with the second optical peak. Spectral and timing analyses of multi-wavelength observations that we describe here, from NuSTAR, Suzaku, Swift/X-Ray Telescope (XRT) + BAT + UltraViolet Optical Telescope (UVOT) (photometry) and optical photometry and spectroscopy, indicate that accretion proceeds through a disk that reaches down to the WD surface. The scenario in which a massive, magnetic WD accretes from a magnetically truncated accretion disk is not supported. For example, none of our data show the minute-time-scale periodic modulations (with tight upper limits from X-ray data) expected from a spinning, magnetic WD. Moreover, the similarity of the UV and X-ray fluxes, as well as the approximate constancy of the hardness ratio within the BAT band, indicate that the boundary layer of the accretion disk remained optically thin to its own radiation throughout the brightening event, during which the rate of accretion onto the WD increased to 6.7 × 10-9M· yr-1 (d/2 kpc)2. For the first time from a WD symbiotic, the NuSTAR spectrum showed a Compton reflection hump at E > 10 keV, due to hard X-rays from the boundary layer reflecting off of the surface of the WD; the reflection amplitude was 0.77 ± 0.21. The best fit spectral model, including reflection, gave a maximum post-shock temperature of kT = 53 ± 4 keV, which implies a WD mass of 1.25 ± 0.02 M·. Although the long-term optical variability in RT Cru is reminiscent of dwarf-novae-type outbursts, the hard X-ray behavior does not correspond to that observed in well-known dwarf nova. An alternative explanation for the brightening events could be that they are due to an enhancement of the accretion rate as the WD travels through the red giant wind in a wide orbit, with a period of about ∼4000 days. In either case, the constancy of the hard X-ray spectrum while the accretion rate rose suggests that the accretion-rate threshold between a mostly optically thin and thick boundary layer, in this object, may be higher than previously thought.Fil: Luna, Gerardo Juan Manuel. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Mukai, K.. National Aeronautics and Space Administration; Estados UnidosFil: Sokoloski, J. L.. Columbia University; Estados UnidosFil: Lucy, A. B.. Columbia University; Estados UnidosFil: Cusumano, G.. Istituto Nazionale di Astrofisica; ItaliaFil: Segreto, A.. Istituto Nazionale di Astrofisica; ItaliaFil: Jaque Arancibia, Marcelo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio. Universidad Nacional de San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio; ArgentinaFil: Nuñez, Natalia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio. Universidad Nacional de San Juan. Instituto de Ciencias Astronómicas, de la Tierra y del Espacio; ArgentinaFil: Puebla, R. E.. Universidad Central del Ecuador; EcuadorFil: Nelson, T.. University of Pittsburgh at Johnstown; Estados UnidosFil: Walter, F. M.. Columbia University; Estados Unido

Potential Utility of Protein Targets of Cysteine-S-Nitrosylation in Identifying Clinical Disease Status in Human Chagas Disease

Trypanosoma cruzi (Tc) infection causes Chagas disease (ChD) presented by dilated cardiomyopathy and heart failure. During infection, oxidative and nitrosative stresses are elicited by the immune cells for control the pathogen; however, excess nitric oxide and superoxide production can result in cysteine S-nitrosylation (SNO) of host proteins that affects cellular homeostasis and may contribute to disease development. To identify the proteins with changes in SNO modification levels as a hallmark of ChD, we obtained peripheral blood mononuclear cells (PBMC) from seronegative, normal healthy (NH, n = 30) subjects, and from seropositive clinically asymptomatic (ChD CA, n = 25) or clinically symptomatic (ChD CS, n = 28) ChD patients. All samples were treated (Asc+) or not-treated (Asc−) with ascorbate (reduces nitrosylated thiols), labeled with the thiol-labeling BODIPY FL-maleimide dye, resolved by two-dimensional electrophoresis (total 166 gels), and the protein spots that yielded significant differences in abundance or SNO level at p-value of ≤ 0.05t−test/Welch/BH were identified by MALDI-TOF/TOF MS or OrbiTrap LC-MS/MS. Targeted analysis of a new cohort of PBMC samples (n = 10–14/group) was conducted to verify the differential abundance/SNO levels of two of the proteins in ChD (vs. NH) subjects. The multivariate adaptive regression splines (MARS) modeling, comparing differences in relative SNO level (Asc−/Asc+ ratio) of the protein spots between any two groups yielded SNO biomarkers that exhibited ≥90% prediction success in classifying ChD CA (582-KRT1 and 884-TPM3) and ChD CS (426-PNP, 582-KRT1, 486-ALB, 662-ACTB) patients from NH controls. Ingenuity Pathway Analysis (IPA) of the SNO proteome dataset normalized to changes in protein abundance suggested the proteins belonging to the signaling networks of cell death and the recruitment and migration of immune cells were most affected in ChD CA and ChD CS (vs. NH) subjects. We propose that SNO modification of the select panel of proteins identified in this study have the potential to identify ChD severity in seropositive individuals exposed to Tc infection

Latitude does not influence cavity entrance orientation of South American avian excavators

In the Northern Hemisphere, several avian cavity excavators (e.g., woodpeckers) orient their cavities increasingly toward the equator as latitude increases (i.e., farther north), and it is proposed that they do so to take advantage of incident solar radiation at their nests. If latitude is a key driver of cavity orientations globally, this pattern should extend to the Southern Hemisphere. Here, we test the prediction that cavities are oriented increasingly northward at higher (i.e., colder) latitudes in the Southern Hemisphere and describe the preferred entrance direction(s) of 1501 cavities excavated by 25 avian species (n = 22 Picidae, 2 Trogonidae, 1 Furnariidae) across 12 terrestrial ecoregions (15°S ? 55°S) in South America. We used Bayesian projected normal mixed-effects models for circular data to examine the influence of latitude, and potential confounding factors, on cavity orientation. Also, a probability model selection procedure was used to simultaneously examine multiple orientation hypotheses in each ecoregion, to explore underlying cavity-orientation patterns. Contrary to predictions, and patterns from the Northern Hemisphere, birds did not orient their cavities more toward the equator with increasing latitude, suggesting that latitude may not be an important underlying selective force shaping excavation behavior in South America. Moreover, unimodal cavity-entrance orientations were not frequent among the ecoregions analyzed (infour ecoregions), whereas bimodal (in five ecoregions) or uniform (in three ecoregions) werealso common, although many of these patterns were not very sharp. Our results highlight the need to include data from under-studied biotas and regions to improve inferences at macroecology scales. Furthermore, we suggest a re-analysis of Northern Hemisphere cavity orientation patterns using a multimodel approach, and a more comprehensive assessment of the role of environmental factors as drivers of cavity orientation at different spatial scales in both hemispheres.Fil: Ojeda, Valeria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Schaaf, Alejandro Alberto. Universidad Nacional de Jujuy. Instituto de Ecorregiones Andinas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Ecorregiones Andinas; ArgentinaFil: Altamirano, Tatiana Edith. University of British Columbia; CanadáFil: Bonaparte, Eugenia Bianca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Bragagnolo, Laura Araceli. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Chazarreta, L.. Secretaría de Ambiente y Desarrallo Sustentable de la Nación; ArgentinaFil: Cockle, Kristina Louise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Dias, R.. Universidade do Brasília; BrasilFil: Di Sallo, Facundo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Ibarra, T.. Pontificia Universidad Católica de Chile; ChileFil: Ippi, Silvina Graciela. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jauregui, Adrian. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Área Zoología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jimenez, Jaime E.. Universidad de Magallanes; ChileFil: Lammertink, J. Martjan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Lopez, F.. Universidad Nacional de La Pampa; ArgentinaFil: Nuñez Montellano, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: de la Peña, Martín. No especifíca;Fil: Rivera, Luis Osvaldo. Universidad Nacional de Jujuy. Instituto de Ecorregiones Andinas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Ecorregiones Andinas; ArgentinaFil: Vivanco, Constanza Guadalupe. Universidad Nacional de Jujuy. Instituto de Ecorregiones Andinas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Ecorregiones Andinas; ArgentinaFil: Santillán, Miguel. Museo de Historia Natural de La Pampa; ArgentinaFil: Soto, G.. Cornell University; Estados UnidosFil: Vergara, P.. Universidad de Santiago de Chile; ChileFil: Politi, Natalia. University of North Texas; Estados Unido

First Colombian Multicentric Newborn Screening for Congenital Toxoplasmosis

Congenital toxoplasmosis can result in permanent sequel as blindness or neurological damage in children and it seems to be more severe in South America than in other continents. There is a lack of information about this frequency in Colombia, where no control program is established, although it is a recognized cause of potentially preventable congenital blindness. We propose the first Colombian multicentric study to determine the frequency and impact of congenital toxoplasmosis. More than 15,000 newborns in seven cities were studied. Newborns were tested at birth by doing a cord blood test for toxoplasmosis. Additionally, children from mothers with history of toxoplasmosis acquired during pregnancy were recalled for a follow-up. The program identified fifteen children otherwise undiagnosed; three of these children died as consequence of congenital toxoplasmosis. The frequency of the congenital infection varied significantly between cities, being higher in Armenia and Florencia, intermediate in Bogota, Bucaramanga and Barranquilla and very low in western cities such as Cucuta and Riohacha. For the first time a significant correlation was found between mean rainfall at the city and the incidence of this congenital infection

A novel genetic variant in PTGS1 affects N-glycosylation of cyclooxygenase-1 causing a dominant-negative effect on platelet function and bleeding diathesis.

During platelet activation, arachidonic acid (AA) is released from membrane phospholipids and metabolized to thromboxane A2 (TXA2) through the actions of cyclooxygenase-1 (COX-1) and TXA2 synthase. Note, TXA2 binds to the platelet TXA2 receptor, causing shape change, secretion and platelet aggregation.1 Also, COX-1 (599aa; 70 kDa) has cyclooxygenase and peroxidase activities and it is functionally active as a homodimer, with each COX-1 monomer consisting of four highly conserved domains: an N-terminal signal peptide, a dimerization domain, a membrane-binding domain (MBD) and a large C-terminal catalytic domain2 (Figure 1A). Irreversible COX-1 inhibition by aspirin is a widely established anti-platelet therapy in cardiovascular disease.Fundación Mutua Madrileña, Grant/Award Number: AP172142019; Fundación Séneca, Grant/Award Number: 19873/GERM/15; Gerencia Regional de Salud, Grant/Award Numbers: 1647/A/17, 2061A/19; Instituto de Salud Carlos III (ISCIII) & Feder, Grant/Award Numbers: CB15/00055, PI17/01966, PI18/00598, PI20/00926, PI17/01311; Junta de Castilla y León; British Heart Foundation, Grant/Award Number: PG/17/40/33028; Ayuda a Grupos de Trabajo en Patología Hemorrágica; Premio López Borrasca 2019; Sociedad Española de Trombosis y Hemostasia

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality