Our Knowledge of High-Mass Star Formation at the Dawn of Herschel

We review the theories and observations of high-mass star formation emphasizing the differences with those of low-mass star formation. We hereafter describe the progress expected to be achieved with Herschel, thanks notably to Key Programmes dedicated to the earliest phases of high-mass star formation.Comment: 16 page

The Nucleolonema of Plant and Animal Cells: A Comparison

Depending on the author and the animal or plant origin of the material under study, the term "nucleolonema" is used in different contexts and thus indicates nucleolar ultrastructures that are different. In this paper, we attempt to clarify this state of affairs and to propose a definition for the plant cell nucleolonema

Three-dimensional electron microscopy of ribosomal chromatin in two higher plants: a cytochemical, immunocytochemical, and in situ hybridization approach.

We report the 3-D arrangement of DNA within the nucleolar subcomponents from two evolutionary distant higher plants, Zea mays and Sinapis alba. These species are particularly convenient to study the spatial organization of plant intranucleolar DNA, since their nucleoli have been previously reconstructed in 3-D from serial ultra-thin sections. We used the osmium ammine-B complex (a specific DNA stain) on thick sections of Lowicryl-embedded root fragments. Immunocytochemical techniques using anti-DNA antibodies and rDNA/rDNA in situ hybridization were also applied on ultra-thin sections. We showed on tilted images that the OA-B stains DNA throughout the whole thickness of the section. In addition, very low quantities of cytoplasmic DNA were stained by this complex, which is now the best DNA stain used in electron microscopy. Within the nucleoli the DNA was localized in the fibrillar centers, where large clumps of dense chromatin were also visible. In the two plant species intranucleolar chromatin forms a complex network with strands partially linked to chromosomal nucleolar-organizing regions identified by in situ hybridization. This study describes for the first time the spatial arrangement of the intranucleolar chromatin in nucleoli of higher plants using high-resolution techniques

Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors

Serine/arginine-rich (SR) proteins are splicing regulators that share a modular structure consisting of one or two N-terminal RNA recognition motif domains and a C-terminal RS-rich domain. We investigated the dynamic localization of the Arabidopsis thaliana SR protein RSZp22, which, as we showed previously, distributes in predominant speckle-like structures and in the nucleolus. To determine the role of RSZp22 diverse domains in its nucleolar distribution, we investigated the subnuclear localization of domain-deleted mutant proteins. Our results suggest that the nucleolar localization of RSZp22 does not depend on a single targeting signal but likely involves different domains/motifs. Photobleaching experiments demonstrated the unrestricted dynamics of RSZp22 between nuclear compartments. Selective inhibitor experiments of ongoing cellular phosphorylation influenced the rates of exchange of RSZp22 between the different nuclear territories, indicating that SR protein mobility is dependent on the phosphorylation state of the cell. Furthermore, based on a leptomycin B- and fluorescence loss in photobleaching-based sensitive assay, we suggest that RSZp22 is a nucleocytoplasmic shuttling protein. Finally, with electron microscopy, we confirmed that RSp31, a plant-specific SR protein, is dynamically distributed in nucleolar cap-like structures upon phosphorylation inhibition. Our findings emphasize the high mobility of Arabidopsis SR splicing factors and provide insights into the dynamic relationships between the different nuclear compartments

Strongly triggered collapse model confront observations

Detailed modelling of individual protostellar condensations, is important to test the various theories. Here we present comparisons between strongly induced collapse models with one young class-0, IRAS4A in the Perseus cloud and one prestellar cloud observed in the Coalsack molecular cloud.Comment: IAU 237, Triggering of star formation in turbulent molecular clouds, eds B. Elmegreen and J. Palou

Analytical theory for the initial mass function: CO clumps and prestellar cores

We derive an analytical theory of the prestellar core initial mass function based on an extension of the Press-Schechter statistical formalism. With the same formalism, we also obtain the mass spectrum for the non self-gravitating clumps produced in supersonic flows. The mass spectrum of the self-gravitating cores reproduces very well the observed initial mass function and identifies the different mechanisms responsible for its behaviour. The theory predicts that the shape of the IMF results from two competing contributions, namely a power-law at large scales and an exponential cut-off (lognormal form) centered around the characteristic mass for gravitational collapse. The cut-off exists already in the case of pure thermal collapse, provided that the underlying density field has a lognormal distribution. Whereas pure thermal collapse produces a power-law tail steeper than the Salpeter value, dN/dlog M\propto M^{-x}, with x=1.35, this latter is recovered exactly for the (3D) value of the spectral index of the velocity power spectrum, n\simeq 3.8, found in observations and in numerical simulations of isothermal supersonic turbulence. Indeed, the theory predicts that x=(n+1)/(2n-4) for self-gravitating structures and x=2-n'/3 for non self-gravitating structures, where n' is the power spectrum index of log(rho). We show that, whereas supersonic turbulence promotes the formation of both massive stars and brown dwarfs, it has an overall negative impact on star formation, decreasing the star formation efficiency. This theory provides a novel theoretical foundation to understand the origin of the IMF and to infer its behaviour in different environments. It also provides a complementary approach and useful guidance to numerical simulations exploring star formation, while making testable predictions.Comment: To appear in Ap

Karstologie et remplissage sédimentaire du gisement pléistocène de Romain-la-Roche (Doubs, France).

19 pagesInternational audienceLe gisement pléistocène du site de Romain-la-Roche (commune de Romain dans le Doubs) correspond au remplissage épikarstique d'un aven affectant les calcaires du Jurassique moyen d'un plateau situé au nord du massif jurassien. Cette cavité, située en dehors de l'extension de la calotte du dernier maximum glaciaire, a été façonnée par un flux hydrologique en liaison avec un drainage de surface qui a vraisemblablement disparu au cours du Pliocène lors d'une phase d'inversion du relief de part et d'autre de la faille normale qui délimite la partie ouest du plateau. Avant son colmatage au cours du Pléistocène la cavité était reliée à un réseau dense de conduits karstiques sous-jacents qui ont été explorés par les spéléologues postérieurement aux opérations de fouilles paléontologiques. La base du remplissage de l'aven est de nature endokarstique et n'a livré aucun matériel paléontologique ou archéologique. Le remplissage épikarstique qui a livré le matériel étudié dans la présente monographie a commencé à se mettre en place au Pléistocène moyen lorsque le toit de la cavité, rattrapé par l'érosion de surface, s'est effondré. L'aven a alors formé un piège karstique d'abord profond et sans échappatoire, puis de plus en plus accessible suite à son comblement et à l'évasement de son ouverture

Analytical theory for the initial mass function: II. Properties of the flow

Recently, Hennebelle and Chabrier (2008) derived an analytical theory for the mass spectrum of non self-gravitating clumps associated with overdensities in molecular clouds and for the initial mass function of gravitationally bound prestellar cores, as produced by the turbulent collapse of the cloud. In this companion paper, we examine the effects of the non-isothermality of the flow, of the turbulence forcing and of local fluctuation of the velocity dispersion, on the mass function. In particular, we investigate the influence of a polytropic equation of state and of the effective adiabatic exponent $\gamma$ and find that it has a drastic influence on the low mass part of the IMF. We also consider a barotropic equation of state (i.e. a piecewise polytropic eos) that mimics the thermal behaviour of the molecular gas and compare the prediction of our theory with the results of numerical simulations and with the observationally-derived IMF, for cloud parameters which satisfy Larson's type relations. We find that for clouds whose density is, at all scales, almost an order of magnitude larger than the density inferred for the CO clumps in the Galaxy, a good agreement is obtained between the theory and the observed IMF, suggesting that star formation preferentially occurs in high density environments. We derive an analytical expression for the IMF which generalizes the expression previously obtained for the isothermal case. This easy-to-implement analytical IMF should serve as a template to compare observational or numerical results with the theory.Comment: accepted for publication in Ap

Calsenilin is required for endocrine pancreas development in zebrafish.

peer reviewedCalsenilin/DREAM/Kchip3 is a neuronal calcium-binding protein. It is a multifunctional protein, mainly expressed in neural tissues and implicated in regulation of presenilin processing, repression of transcription, and modulation of A-type potassium channels. Here, we performed a search for new genes expressed during pancreatic development and have studied the spatiotemporal expression pattern and possible role of calsenilin in pancreatic development in zebrafish. We detected calsenilin transcripts in the pancreas from 21 somites to 39 hours postfertilization stages. Using double in situ hybridization, we found that the calsenilin gene was expressed in pancreatic endocrine cells. Loss-of-function experiments with anti-calsenilin morpholinos demonstrated that injected morphants have a significant decrease in the number of pancreatic endocrine cells. Furthermore, the knockdown of calsenilin leads to perturbation in islet morphogenesis, suggesting that calsenilin is required for early islet cell migration. Taken together, our results show that zebrafish calsenilin is involved in endocrine cell differentiation and morphogenesis within the pancreas