Demonstração da parede celular de bactérias em preparações coradas

The method proposed by Robinow & Murray (Scanga, 1959), to demonstrate the cell wall of bacteria is quite seletive for Gram positives but, when applied to Gram negatives, all the cell becomes evenly stained. The method proposed by Knaysi (1941) althought offering a better contrast is of very delicate performance, to be practical. In this paper, the AA. present a new staining method in order to offer a good contrast between the cell wall and cytoplasmic material. Essentially it consists in turning citoplasmic material unstainable by treatment of the cells with a acid mordent. The subsequent stainning with malachite green or Victoria blue gives a very good contrast. The method can be applied either to Gram positive or Gram negative bacteria.O artigo apresenta resumo em inglês

Esporotricose em cães

Os autores apresentam dois casos de esporotricose em cães, micose ainda não assinalada nessa espécie, em S. Paulo. O processo, em ambos os casos, teve evolução crônica, não afetou aparentemente o estado geral dos animais e caracterizou-se por lesões cutâneas de caráter úlcero-gomoso em diferentes graus de desenvolvimento, configuradas por formações nodulares de consistência diversa, áreas ulceradas e trajetos fistulosos dos quais vazava secreção purulenta. Havia reação linfática bem evidente acusada pelo pronunciado aumento de volume dos gânglios regionais. Em esfregaços irreparados com o pus das fístulas ou cor. raspados das úlceras, corados pelo Gram, o parasita foi facilmente reconhecido pela sua "forma em charuto". Foram obtidas culturas típicas em Sabouraud maltosado e glicosado, em temperatura ambiente e a 37°C. Em um dos casos, o tratamento com griseofulvina "(Grifulvin)" não teve êxito. A administração de iodeto de potássio reverteu em rápida melhora, aparentando cura, porém houve recidiva após alguns meses.For the first time in Brazil Sporothricosis is described in dogs. In the two cases studied the disease was characterized by the appearance of cutaneous nodes of different consistences and sometimes ulcerous, covered with dark scabs. Fistulous openings complete the lesion and purulent discharge could be observed as well as involvement of the drainage limph nodes. In the contents of the nodes, in the purulent exudates of the fistulae and the scrapings of the ulcers, there were many parasites, in characteristics "cigar shape". Cultures were obtained easily at room temperature and at 37 °C. The disease followed a chronic course and no marked constitucional symptoms were noted. In one of the cases the treatment with Griseofulvina was not successfull but the ministration of potassium iodine resulted in a quick but false recovery, because after some months there was a recrudescency

Estudo sobre estafilococos patogênicos isolados de cães

According to the results of Rajulu & col. (1960) about bacteriphage relations between strains of staphylococci isolated from dogs and men, the A. A. reviewed their data and found that material from cases of furunculosis which they have tested, were many limes related to students, teachers or workers that were in frequent contact with dogs. The cultures from swabs of skin and nostrils of 113 dogs and 42 students revealed that all dogs were carriers of pathogenic staphylococci, on the skin, nostrils or both. Out of 42 students, 10 were carriers. The strains isolated from dogs were all of the “albus” type and alpha, beta or alpha-beta hemolysin producers. The strains isolated from students were all of the “aureus” type and only alpha hemolysin producers. All of the strains fermented dextrose but there was no uniformity as to manitol and gelatin. During the work one of the A. A. presented an apicitis of tooth, from which it was obtained, in pure culture, an “albus” type betahemolytic an coagulase-positive staphylococus (Phage type 29). None of the strains of canine origin could be phage typed utilizing human or bovine series of phages.O artigo apresenta resumo em inglês

Aspergilose aviária

O artigo apresenta resumo em inglês.Aspergillosis caused by Aspergillus fumigatus was observed in “juriti” (Leptotilla rufaxilla) and in “cacatua” (Cacatua moluccensis). These birds were found dead on their cages at the Parque Zoológico, São Paulo — Brasil. Necroscopic examination revealed nodular ball shapped formations distributed along the pulmonar parenchyma, air sacks and mesenteric serosa. Pure cultures of Aspergillus fumigatus were obtained on Sabouraud’s dextrose ágar in both cases, and suspensions of these cultures caused death in rabbits, guinea pigs and pigeons when give by the intra-venous route. The fungs was reisolated from these animals. Addition to water and meal of Lugol’s solutions was recommended, after thorough desinfection of the cages to prevent spread of infection to susceptibbles. No further cases were observed. The birds refered in this work were not included yet in the list of susceptibles to Aspergillosis in brazilian scientific literature

Esporotricose em cães e gatos

The authors present observations on 20 cases of sporotrichosis, 12 in dogs and 8 in cats, during 18 months of observation. They conclude: 1) In cats and dogs, sporotrichosis is much more frequent than literature indications. 2) Laboratory diagnosis can't present troubles, since the agent is relatively abundant in the lesions. 3) Evolution of the disease is very slow and it seems not to affect the general conditions of the patients, since it limits it's action to the cutaneous tissue. 4) Transmission by direct contact seems to be very difficult.O artigo apresenta resumo em inglês

Pathogenic variants in glutamyl-tRNAGln amidotransferase subunits cause a lethal mitochondrial cardiomyopathy disorder.

Mitochondrial protein synthesis requires charging mt-tRNAs with their cognate amino acids by mitochondrial aminoacyl-tRNA synthetases, with the exception of glutaminyl mt-tRNA (mt-tRNAGln). mt-tRNAGln is indirectly charged by a transamidation reaction involving the GatCAB aminoacyl-tRNA amidotransferase complex. Defects involving the mitochondrial protein synthesis machinery cause a broad spectrum of disorders, with often fatal outcome. Here, we describe nine patients from five families with genetic defects in a GatCAB complex subunit, including QRSL1, GATB, and GATC, each showing a lethal metabolic cardiomyopathy syndrome. Functional studies reveal combined respiratory chain enzyme deficiencies and mitochondrial dysfunction. Aminoacylation of mt-tRNAGln and mitochondrial protein translation are deficient in patients' fibroblasts cultured in the absence of glutamine but restore in high glutamine. Lentiviral rescue experiments and modeling in S. cerevisiae homologs confirm pathogenicity. Our study completes a decade of investigations on mitochondrial aminoacylation disorders, starting with DARS2 and ending with the GatCAB complex