Cotton fabric coated with conducting polymers and its application in monitoring of carnivorous plant response

The paper describes the electrical plant response to mechanical stimulation monitored with the help of conducting polymers deposited on cotton fabric. Cotton fabric was coated with conducting polymers, polyaniline or polypyrrole, in situ during the oxidation of respective monomers in aqueous medium. Thus, modified fabrics were again coated with polypyrrole or polyaniline, respectively, in order to investigate any synergetic effect between both polymers with respect to conductivity and its stability during repeated dry cleaning. The coating was confirmed by infrared spectroscopy. The resulting fabrics have been used as electrodes to collect the electrical response to the stimulation of a Venus flytrap plant. This is a paradigm of the use of conducting polymers in monitoring of plant neurobiology.Web of Science164art. no. 49

The effect of freezing temperature on physiological traits in sunflower

This study was conducted to identify the physiological mechanisms associated with the resistance and tolerance of young sunflower plants to freezing temperatures. The effect of overnight temperature –3°C on the maximal quantum efficiency of PSII (Fv/Fm), the relative electrolyte leakage (REL) and the osmotic potential (Ψπ) was determined in five genotypes of sunflower: C33, C98, C124 and C148 were chosen from the population of recombinant inbred lines (RILs) based on contrasted responses to low temperature, and a wild genotype 2603 that was chosen for its ability to maintain activities in cold conditions. The night temperature –3°C over the course of 10 h caused an immediate significant decrease of Fv/Fm in C33, C98, C124 and C148. In the case of genotype C98, the effect of this freezing temperature was manifested by a significant increase of REL. Significant changes of Ψπ, as a reaction to the effect of freezing temperatures, were not found in any of the monitored genotypes. The measurements of the physiological traits after 5 days of regeneration indicated the renewal of integrity of cellular structures and an increase of PSII reaction centre efficiency in all monitored genotypes. From the point of view of tolerance or sensitivity, the wild genotype 2603 showed itself as tolerant towards the tested freezing temperature, displaying insignificant differences with control plants in all monitored traits. Genotype C98 appears to be the most sensitive from the monitored set, with evident changes in two traits signalling frost damage

The Invasive Box Tree Moth Five Years after Introduction in Slovakia: Damage Risk to Box Trees in Urban Habitats

The box tree moth Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) is an invasive species in Europe and a serious pest of box trees (Buxus spp.). In Slovakia, Central Europe, it was first reported in 2012 within the low elevation region with a warm climate. We hypothesize that the cold mountain region of Slovakia would provide less suitable conditions for the spread of this species, indicated by no or only slight damage to box trees. Five years after C. perspectalis was first recorded in Slovakia, we assessed the probability of occurrence of the moth and the probability of damage to box trees (Buxus sempervirens) by its larvae, using temperature and altitude data as predictors. In June and July 2017, at 156 locations (towns and villages) between the altitudes of 109 and 888 m, we recorded damage and categorized the intensity of damage to box trees by C. perspectalis using a four-point scale. Box trees infested by C. perspectalis were recorded in most locations at altitudes between 110 and 400 m with the mean annual temperature varying between 10.5 and 7.9 °C. High damage to box trees was found in locations up to 340 m a.s.l. characterized by mean annual temperatures above 8.5 °C. Our results suggested high probability (>60%) of any damage to box trees for the area up to approximately 300 m a.s.l. (mean annual temperature above 8.4 °C), and high probability (>60%) of high damage for the area up to approximately 250 m a.s.l. (mean annual temperature above 9 °C). The area where damage to box trees was predicted using the altitude showed great overlap with the area predicted using the mean annual temperature. The area with the probability of any damage was only slightly larger than the area with the probability of high damage

Laboratory diagnosis of Trichinella spp. in animals and its importance for prevention of human trichinelosis

Department of Pharmacology and ToxicologyKatedra farmakologie a toxikologieFaculty of Pharmacy in Hradec KrálovéFarmaceutická fakulta v Hradci Králov

Annual Report 2020

Výroční zpráva NZM za rok 202

Formativní hodnocení a jeho zavádění do praxe ZŠ Dolní Lhota

This thesis deals with the topic of formative evaluation. In the theoretical part I define the term formative evaluation and related terms. The practical part describes how the formative evaluation was applied at a primary school in Dolní Lhota. The aim of this thesis is to show how it is possible to apply formative evaluation at primary schools

Fiscal decentralization determinants: analysis of the EU countries' clustered sample in period 1995-2015

Fiscal decentralization as a shift of decision-making powers from the central level of government to lower government levels is an object of wide research. In this field there is a discussion ranging from the eligibility of fiscal decentralization, its advantages and threats, to searching for the fiscal decentralization´s impact on certain macroeconomic indicators. Research focusing on investigating for fiscal decentralization determinants has not clear answer to this question. It considers various indicators often with their ambiguous impact on fiscal decentralization. In this paper, the problem of fiscal decentralization determinants is investigated on the EU countries sample in period 1995-2015. EU countries are divided into groups according to their fiscal decentralization average degree using the cluster analysis. Fiscal decentralization different measures refer on the expenditure, revenue, tax and grant decentralization. For each cluster a panel model is estimated to reveal an influence of selected variables on fiscal decentralization, while fiscal decentralization as dependent variable is measured by chosen various measure. Sensitivity of the estimation results on the fiscal decentralization measurement is obvious. Obtained results partially support assumptions given on basics of related literature. Significant appearance of the economy openness variable, GDP per capita growth and inflation rate, public and local debt variable and variable based on dependency ratio, and population density is influenced by construction of the fiscal decentralization indicator. It might explain the disunited character of observed FEM and REM results, additionally the turn of coefficient sign comparing fiscal decentralization measures postulates certain common tendencies revealing the fiscal decentralization character and conditions