Matlab parallel codes for 3D slope stability benchmarks

This contribution is focused on a description of implementation details for solver related to the slope stability benchmarks in 3D. Such problems are formulated by the standard elastoplastic models containing the Mohr-Coulomb yield criterion and by the limit analysis of collapse states. The implicit Euler method and higher order finite elements are used for discretization. The discretized problem is solved by non-smooth Newton-like methods in combination with incremental methods of limit load analysis. In this standard approach, we propose several innovative techniques. Firstly, we use recently developed sub-differential based constitutive solution schemes. Such an approach is suitable for non-smooth yield criteria, and leads better return-mapping algorithms. For example, a priori decision criteria for each return-type or simplified construction of consistent tangent operators are applied. The parallel codes are developed in MATLAB using Parallel Computing Toolbox. For parallel implementation of linear systems, we use the TFETI domain decomposition method. It is a non-overlapping method where the Lagrange multipliers are used to enforce continuity on the subdomain interfaces and satisfaction of the Dirichlet boundary conditions

Building rapid transit in Canada: the problem of governance

Anuario de Derecho Municipal 2020 Madrid, 2021. ISSN: 1888-7392. Nº 14: 243-258 Building rapid transit in Canada: the problem of governance Martin Horak* University of Western Ontario ABSTRACT Canadian cities have seen a boom in the construction of rapid transit infrastructure in recent years, fueled by the rise of financial support for transit from the federal government and the provinces. However, the extent to which individual cities have been able to productively harness this new financial support varies greatly. This study compares the recent development of rapid transit infrastructure in two of Canada’s largest metropolitan areas, Toronto and Vancouver. It finds that while both cities have recently developed regional transportation authorities to manage large transit investments, in Toronto the development of rapid transit has been highly contentious, marked by frequent changes in plan and the repeated cancellation and deferral of transit projects, while in Vancouver, the development of rapid transit has been much more consensual and orderly. The study introduces an analytical framework that interprets these different outcomes as the result of dissimilar institutional environments in the two cities, which vary in the extent to which they insulate long-range planning and decision-making from efforts by politicians to harness rapid transit decisions for short-term electoral advantag

Endocrinology: Clinical significance of invisible or partially visible luteinizing hormone

It is well known that luteinizing hormone (LH), like many other glycoproteins, is heterogeneous and presents several circulating isoforms. Recently, new sensitive immunometric assays measuring intact LH were developed. These assays have been found to underestimate or to be incapable of recognizing LH in some patients. This study was undertaken to determine the prevalence of such cases and to define their clinical characteristics. We compared three LH assays using as capture antibodies either a monoclonal antibody that reacts exclusively with intact LH (ES 600 Boehringer, Stratus Baxter) or a monoclonal antibody against the β subunit of LH (IMX Abbott). In 17% of 90 patients tested, ES 600 measured > 50% lower LH concentrations when compared with the EVIX. Moreover, in two cases LH was not detectable by ES 600 or Stratus, whereas it was normal with the EVIX. We found another five such cases and discuss here the clinical data and results of different hormone measurements in these seven cases of ‘invisible LH'. Although bioactive LH (mouse Leydig cell assay) was normal, the existence of low or even undetectable LH was clinically confusing and led to expensive complementary investigations such as gonadotrophin-releasing hormone analogue tests and magnetic resonance imaging. The uses and limitations of these assays are illustrated by different clinical situations in which the results of the different assays have been misleadin

Municipal Resource Guide to Leading Practices in Cost Savings

Ontario municipalities of all sizes face pressure to “do more with less.” Commissioned by the Ontario Ministry of Municipal Affairs, this Resource Guide provides municipal officials with single-source information about a range of current leading practices in cost savings in small and mid-sized Ontario municipalities. Municipalities across the province are continually developing innovative practices that save costs without incurring service level reductions. Most of these practices involve small-scale initiatives that result in modest savings. Yet even modest savings add up over time, and multiple small initiatives in a single municipality can make a big difference. In addition, by providing an opportunity to re-think established service provision practices, these initiatives often have significant non-monetary benefits as well. This Guide, based on a year of research by a team at Western University, presents detailed profiles of 14 selected cases of leading practices in cost savings. The case studies come from municipalities of varying sizes in all regions of the province, and profile leading practices in a wide variety of service fields. In addition to these cases, the Guide presents a reference compendium of 159 cost-savings recommendations from recent Municipal Service Delivery Reviews. The Guide is intended to serve as a source of ideas and inspiration for Ontario’s local officials as they seek to provide the best possible services to their residents in challenging fiscal times.https://ir.lib.uwo.ca/urbancentre-reports/1000/thumbnail.jp

Novel insights into the genetic diversity of Balantidium and Balantidium-like cyst-forming ciliates

Balantidiasis is considered a neglected zoonotic disease with pigs serving as reservoir hosts. However, Balantidium coli has been recorded in many other mammalian species, including primates. Here, we evaluated the genetic diversity of B. coli in non-human primates using two gene markers (SSrDNA and ITS1-5.8SDNA-ITS2). We analyzed 49 isolates of ciliates from fecal samples originating from 11 species of captive and wild primates, domestic pigs and wild boar. The phylogenetic trees were computed using Bayesian inference and Maximum likelihood. Balantidium entozoon from edible frog and Buxtonella sulcata from cattle were included in the analyses as the closest relatives of B. coli, as well as reference sequences of vestibuliferids. The SSrDNA tree showed the same phylogenetic diversification of B. coli at genus level as the tree constructed based on the ITS region. Based on the polymorphism of SSrDNA sequences, the type species of the genus, namely B. entozoon, appeared to be phylogenetically distinct from B. coli. Thus, we propose a new genus Neobalantidium for the homeothermic clade. Moreover, several isolates from both captive and wild primates (excluding great apes) clustered with B. sulcata with high support, suggesting the existence of a new species within this genus. The cysts of Buxtonella and Neobalantidium are morphologically indistinguishable and the presence of Buxtonella-like ciliates in primates opens the question about possible occurrence of these pathogens in humans

Cholesterol modulates presynaptic and postsynaptic properties of excitatory synaptic transmission

Cholesterol is a structural component of cellular membranes particularly enriched in synapses but its role in synaptic transmission remains poorly understood. We used rat hippocampal cultures and their acute cholesterol depletion by methyl-β-cyclodextrin as a tool to describe the physiological role of cholesterol in glutamatergic synaptic transmission. Cholesterol proved to be a key molecule for the function of synapses as its depletion resulted in a significant reduction of both NMDA receptor (NMDAR) and AMPA/kainate receptor-mediated evoked excitatory postsynaptic currents (eEPSCs), by 94% and 72%, respectively. We identified two presynaptic and two postsynaptic steps of synaptic transmission which are modulated by cholesterol and explain together the above-mentioned reduction of eEPSCs. In the postsynapse, we show that physiological levels of cholesterol are important for maintaining the normal probability of opening of NMDARs and for keeping NMDARs localized in synapses. In the presynapse, our results favour the hypothesis of a role of cholesterol in the propagation of axonal action potentials. Finally, cholesterol is a negative modulator of spontaneous presynaptic glutamate release. Our study identifies cholesterol as an important endogenous regulator of synaptic transmission and provides insight into molecular mechanisms underlying the neurological manifestation of diseases associated with impaired cholesterol synthesis or decomposition

How dose of biochar and biochar with nitrogen can improve the parameters of soil organic matter and soil structure?

Biochar application to agricultural soils has a significant potential to influence soil resource availability and thus crop performance. A factorial experiment investigating effects of different biochar application rates combined with nitrogen fertilizer was conducted in field conditions on a Haplic Luvisol. The aim of this study was to evaluate the effects of biochar and biochar combined with fertilization on soil organic matter and soil structure parameters. The treatments comprised combinations of biochar application of 0, 10 and 20 t ha−1 (B0, B10 and B20) and 0, 40 and 80 kg N ha−1 of nitrogen fertilizer (N0, N40, N80) applied in a full-factorial design. Biochar application rate of 20 t ha−1 significantly increased soil organic carbon content (SOC) and non-labile carbon content (CNL), but decreased carbon lability (LC). The addition of biochar at 10 t ha−1 together with 40 and 80 kg N ha−1 significantly increased the values of SOC and CNL. On the other hand, B10N80 treatment resulted in a considerable decrease of carbon lability (LC). Overall, the lowest average content of water-stable micro-aggregates was found in the B20N80 treatment and then with B10N0 < B20N40 < B20N0 < B10N80 < B0N0 < B10N40. Biochar applied at 20 t ha−1 increased the critical level of soil organic matter and decreased the crusting index

Crucial Role of Interferon Consensus Sequence Binding Protein, but neither of Interferon Regulatory Factor 1 nor of Nitric Oxide Synthesis for Protection Against Murine Listeriosis

Listeria monocytogenes is widely used as a model to study immune responses against intracellular bacteria. It has been shown that neutrophils and macrophages play an important role to restrict bacterial replication in the early phase of primary infection in mice, and that the cytokines interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) are essential for protection. However, the involved signaling pathways and effector mechanisms are still poorly understood. This study investigated mouse strains deficient for the IFN-dependent transcription factors interferon consensus sequence binding protein (ICSBP), interferon regulatory factor (IRF)1 or 2 for their capacity to eliminate Listeria in vivo and in vitro and for production of inducible reactive nitrogen intermediates (RNI) or reactive oxygen intermediates (ROI) in macrophages. ICSBP−/− and to a lesser degree also IRF2−/− mice were highly susceptible to Listeria infection. This correlated with impaired elimination of Listeria from infected peritoneal macrophage (PEM) cultures stimulated with IFN-γ in vitro; in addition these cultures showed reduced and delayed oxidative burst upon IFN-γ stimulation, whereas nitric oxide production was normal. In contrast, mice deficient for IRF1 were not able to produce nitric oxide, but they efficiently controlled Listeria in vivo and in vitro. These results indicate that (a) the ICSBP/IRF2 complex is essential for IFN-γ–mediated protection against Listeria and that (b) ROI together with additional still unknown effector mechanisms may be responsible for the anti-Listeria activity of macrophages, whereas IRF1-induced RNI are not limiting

Fluvastatin in the first-line therapy of acute coronary syndrome: results of the multicenter, randomized, double-blind, placebo-controlled trial (the FACS-trial)

<p>Abstract</p> <p>Background</p> <p>Statins have been proved to be effective in reduction of mortality and morbidity when started in the early secondary prevention in stabilized patients after acute coronary syndrome (ACS). The safety and efficacy of statin administration directly in the first-line therapy in unstable ACS patients is not clear. The aim of our study was, therefore, to assess the effect of statin treatment initiated immediately at hospital admission of patients with ACS.</p> <p>Methods</p> <p>The trial was stopped prematurely after enrollment of one hundred and fifty-six patients with ACS that were randomized at admission to fluvastatin 80 mg (N = 78) or placebo (N = 78). Study medication was administered immediately after randomization and then once daily for 30 days; all patients were then encouraged to continue in open-label statin therapy and at the end of one-year follow-up 75% in the fluvastatin group and 78% in the placebo group were on statin therapy.</p> <p>Results</p> <p>We did not demonstrate any difference between groups in the level of C-reactive protein, interleukin 6, and pregnancy-associated plasma protein A on Day 2 and Day 30 (primary endpoint). Fluvastatin-therapy, however, significantly reduced one-year occurrence of major adverse cardiovascular events (11.5% vs. 24.4%, odds ratio (OR) 0.40, 95% CI 0.17-0.95, P = 0.038). This difference was caused mainly by reduction of recurrent symptomatic ischemia (7.7% vs. 20.5%, OR 0.32, 95% CI 0.12-0.88, P = 0.037).</p> <p>Conclusions</p> <p>This study failed to prove the effect of fluvastatin given as first-line therapy of ACS on serum markers of inflammation and plaque instability. Fluvastatin therapy was, however, safe and it may reduce cardiovascular event rate that supports immediate use of a statin in patients admitted for ACS.</p> <p>Trial registration</p> <p>NCT00171275</p

Hyperinvasive approach to out-of hospital cardiac arrest using mechanical chest compression device, prehospital intraarrest cooling, extracorporeal life support and early invasive assessment compared to standard of care. A randomized parallel groups ...

Out of hospital cardiac arrest (OHCA) has a poor outcome. Recent non-randomized studies of ECLS (extracorporeal life support) in OHCA suggested further prospective multicenter studies to define population that would benefit from ECLS. We aim to perform a prospective randomized study comparing prehospital intraarrest hypothermia combined with mechanical chest compression device, intrahospital ECLS and early invasive investigation and treatment in all patients with OHCA of presumed cardiac origin compared to a standard of care. Methods This paper describes methodology and design of the proposed trial. Patients with witnessed OHCA without ROSC (return of spontaneous circulation) after a minimum of 5 minutes of ACLS (advanced cardiac life support) by emergency medical service (EMS) team and after performance of all initial procedures (defibrillation, airway management, intravenous access establishment) will be randomized to standard vs. hyperinvasive arm. In hyperinvasive arm, mechanical compression device together with intranasal evaporative cooling will be instituted and patients will be transferred directly to cardiac center under ongoing CPR (cardiopulmonary resuscitation). After admission, ECLS inclusion/exclusion criteria will be evaluated and if achieved, veno-arterial ECLS will be started. Invasive investigation and standard post resuscitation care will follow. Patients in standard arm will be managed on scene. When ROSC achieved, they will be transferred to cardiac center and further treated as per recent guidelines. Primary outcome 6 months survival with good neurological outcome (Cerebral Performance Category 1–2). Secondary outcomes will include 30 day neurological and cardiac recovery. Discussion Authors introduce and offer a protocol of a proposed randomized study comparing a combined “hyper invasive approach” to a standard of care in refractory OHCA. The protocol is opened for sharing by other cardiac centers with available ECLS and cathlab teams trained to admit patients with refractory cardiac arrest under ongoing CPR. A prove of concept study will be started soon. The aim of the authors is to establish a net of centers for a multicenter trial initiation in future