Inhibitory potential of some selected essential oils and their main components on the growth and quorum-sensing based pigment production of Serratia marcescens

In this study the antibacterial potential of ten essential oils (EOs) and their main compounds against the development and quorum sensing (QS) mechanisms of the opportunistic bacterium Serratia marcescens was determined. The growth and QS inhibitory effect was evaluated by paper disc diffusion assay. The effect of EOs and components on QS-regulated prodigiosin biosynthesis was also studied. The results of our study indicated that some of the investigated EOs influenced the development and the QS-based activity of S. marcescens. Oregano and thyme oils showed the most pronounced antibacterial effect and had the strongest anti-QS potential. From the main oil compounds the phenolics, eugenol, carvacrol and thymol, proved to be efficient growth inhibitors. While eugenol and carvacrol had also a strong negative influence on AHL-mediated QS-systems in low concentrations, thymol was not effective in QS inhibition

Rational Dual Certificates for Weighted Sums-of-Squares Polynomials with Boundable Bit Size

In (Davis and Papp, 2022), the authors introduced the concept of dual certificates of sum-of-squares polynomials, which are vectors from the dual cone of the cone of weighted sums of squares (WSOS) polynomials that can be interpreted as WSOS nonnegativity certificates. This initial theoretical work showed that for every polynomial in the interior of a WSOS cone, there exists a rational dual certificate proving that the polynomial is WSOS. In this article, we analyze the complexity of rational dual certificates of WSOS polynomials by bounding the bit sizes of integer dual certificates as a function of parameters such as the degree and the number of variables of the polynomials, or their distance from the boundary of the cone. After providing a general bound, we explore a number of special cases, such as univariate polynomials nonnegative over the real line or a bounded interval, represented in different commonly used bases. We also provide an algorithm which runs in rational arithmetic and computes a rational certificate with boundable bit size for a WSOS lower bound of the input polynomial.Comment: Submitted for publicatio

Perfil epidemiológico das pacientes com cancer de colo de útero atendidas no Hospital Universitário: análise de 116 casos

Trabalho de Conclusão de Curso - Universidade Federal de Santa Catarina. Curso de Medicina. Departamento de Tocoginecologia

Kinetic studies of Candida parapsilosis phagocytosis by macrophages and detection of intracellular survival mechanisms

Peer reviewedPublisher PD

The search for the Holy Grail: autoantigenic targets in primary sclerosing cholangitis associated with disease phenotype and neoplasia

L

Comparação entre a imuno-separação magnética, acoplada à imunofluorescência, e as técnicas de Faust et al. e de Lutz para o diagnóstico de cistos de Giardia lamblia em fezes humanas

No presente trabalho, o desempenho da técnica de Imunoseparação Magnética, acoplada à Imunofluorescência (IMS-IFA), foi comparado com aqueles das técnicas parasitológicas de FAUST et al. e de Lutz na detecção de cistos de Giardia lamblia em fezes humanas. Foram processadas 127 amostras de fezes pelas três técnicas paralelamente e a detecção de cistos foi de 27,5% para IMS-IFA e de 15,7% para as técnicas de FAUST et al. e de Lutz concomitantemente. A análise dos resultados mostrou maior sensibilidade da IMS-IFA na detecção de cistos de G. lamblia quando comparada aos métodos de FAUST et al. e Lutz. A utilização desta metodologia como procedimento de rotina proporciona o processamento de várias amostras simultaneamente, além de aumentar a recuperação de cistos de G. lamblia e reduzir o tempo de estocagem das amostras.In the present study, the performance of Immunomagnetic Separation technique, coupled with Immunofluorescence (IMS-IFA), was compared with the FAUST et al. and Lutz parasitological techniques for the detection of Giardia lamblia cysts in human feces. One hundred and twenty-seven samples were evaluated by the three techniques at the same time showing a rate of cyst detection of 27.5% by IMS-IFA and 15.7% by both Faust et al. and Lutz techniques. Data analysis showed a higher sensitivity of IMS-IFA for the detection of G. lamblia cysts in comparison with the techniques of FAUST et al. and Lutz. The use of this methodology as a routine procedure enables the processing of many samples simultaneously, in order to increase recovery rate of G. lamblia cysts and reduce the time of sample storage

PREDICT identifies precipitating events associated with the clinical course of acutely decompensated cirrhosis

Background & Aims: Acute decompensation (AD) of cirrhosis may present without acute-on-chronic liver failure (ACLF) (AD No ACLF), or with ACLF (AD-ACLF), defined by organ failure(s). Herein, we aimed to analyze and characterize the precipitants leading to both of these AD phenotypes. Methods: The multicenter, prospective, observational PREDICT study (NCT03056612) included 1,273 non-electively hospitalized patients with AD (No ACLF = 1,071; ACLF = 202). Medical history, clinical data and laboratory data were collected at enrolment and during 90-day follow-up, with particular attention given to the following characteristics of precipitants: induction of organ dysfunction or failure, systemic inflammation, chronology, in tensity, and relationship to outcome. Results: Among various clinical events, 4 distinct events were precipitants consistently related to AD: proven bacterial in fections, severe alcoholic hepatitis, gastrointestinal bleeding with shock and toxic encephalopathy. Among patients with precipitants in the AD-No ACLF cohort and the AD-ACLF cohort (38% and 71%, respectively), almost all (96% and 97%, respec tively) showed proven bacterial infection and severe alcoholic hepatitis, either alone or in combination with other events. Survival was similar in patients with proven bacterial infections or severe alcoholic hepatitis in both AD phenotypes. The number of precipitants was associated with significantly increased 90- day mortality and was paralleled by increasing levels of surro gates for systemic inflammation. Importantly, adequate first-line antibiotic treatment of proven bacterial infections was associated with a lower ACLF development rate and lower 90-day mortality. Conclusions: This study identified precipitants that are signifi cantly associated with a distinct clinical course and prognosis in patients with AD. Specific preventive and therapeutic strategies targeting these events may improve outcomes in patients with decompensated cirrhosis. Lay summary: Acute decompensation (AD) of cirrhosis is char acterized by a rapid deterioration in patient health. Herein, we aimed to analyze the precipitating events that cause AD in pa tients with cirrhosis. Proven bacterial infections and severe alcoholic hepatitis, either alone or in combination, accounted for almost all (96-97%) cases of AD and acute-on-chronic liver fail ure. Whilst the type of precipitant was not associated with mortality, the number of precipitant(s) was. This study identified precipitants that are significantly associated with a distinct clinical course and prognosis of patients with AD. Specific pre ventive and therapeutic strategies targeting these events may improve patient outcomes

Ökológiai gazdálkodás melléklet - 2012. április - május

Az Őstermelő című lap ökológiai gazdálkodás mellékletét az ÖMKi és Hungária Öko Garancia munkatársai szerkesztették. E szám mellékletében az alábbi témákról olvashatnak: - Baktériumkészítmények hatása az ökológiai kukoricatermesztésben - Paradicsom tájfajták összehasonlító vizsgálata - Szőlősorköz-takarónövényzet vizsgálatok együttműködésben a gazdálkodókkal - 6. Európai Ökológiai Gazdálkodási Kongresszus: "Intelligens váltás egy fenntartható KAP felé" - Organic Marketing Forum - Hogyan álljunk át okosan ökológiai gazdálkodásra? Hasznos tippek az ellenőrző szervezettő

Identification of chitinase-3-like protein 1 as a novel neutrophil antigenic target in Crohn’s disease

Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD