Alonso, Maria F

Bain, Judith M

Erwig, Lars Peter

Gácser, Attila

Jankovics, Ferenc

Papp, Csaba

Tóth, Adél

Tóth, Renáta

Vágvölgyi, Csaba

Frontiers in Microbiology

English

PubMed

Even though the number of Candida infections due to non-albicans species like C. parapsilosis has been increasing, little is known about their pathomechanisms. Certain aspects of C. parapsilosis and host interactions have already been investigated; however we lack information about the innate cellular responses toward this species. The aim of our project was to dissect and compare the phagocytosis of C. parapsilosis to C. albicans and to another Candida species C. glabrata by murine and human macrophages by live cell video microscopy. We broke down the phagocytic process into three stages: macrophage migration, engulfment of fungal cells and host cell killing after the uptake. Our results showed increased macrophage migration toward C. parapsilosis and we observed differences during the engulfment processes when comparing the three species. The engulfment time of C. parapsilosis was comparable to that of C. albicans regardless of the pseudohypha length and spatial orientation relative to phagocytes, while the rate of host cell killing and the overall uptake regarding C. parapsilosis showed similarities mainly with C. glabrata. Furthermore, we observed difference between human and murine phagocytes in the uptake of C. parapsilosis. UV-treatment of fungal cells had varied effects on phagocytosis dependent upon which Candida strain was used. Besides statistical analysis, live cell imaging videos showed that this species similarly to the other two also has the ability to survive in host cells via the following mechanisms: yeast replication, and pseudohypha growth inside of phagocytes, exocytosis of fungal cells and also abortion of host cell mitosis following the uptake. According to our knowledge this is the first study that provides a thorough examination of C. parapsilosis phagocytosis and reports intracellular survival mechanisms associated with this species

Alonso, Maria F.

Bain, Judith M.

Erwig, Lars-Peter

Repository of the Academy's Library

Kinetic studies of Candida parapsilosis phagocytosis by macrophages and detection of intracellular survival mechanisms

RenÃ¡ta TÃ³th

AdÃ©l TÃ³th

Csaba Papp

Ferenc Jankovics

Csaba VÃ¡gvÃ¶lgyi

Maria F. Alonso

Judith M. Bain

Lars-Peter Erwig

Attila GÃ¡cser

Crossref

Even though the number of Candida infections due to non-albicans species like C. parapsilosis has been increasing, little is known about their pathomechanisms. Certain aspects of C. parapsilosis and host interactions have already been investigated; however we lack information about the innate cellular responses towards this species. The aim of our project was to dissect and compare the phagocytosis of C. parapsilosis to C. albicans and to another Candida species C. glabrata by murine and human macrophages by live cell video microscopy.  We broke down the phagocytic process into three stages: macrophage migration, engulfment of fungal cells and host cell killing after the uptake. Our results showed increased macrophage migration towards C. parapsilosis and we observed differences during the engulfment processes when comparing the three species. The engulfment time of C. parapsilosis was comparable to that of C. albicans regardless of the pseudohypha length and spatial orientation relative to phagocytes, while the rate of host cell killing and the overall uptake regarding C. parapsilosis showed similarities mainly with C. glabrata. Furthermore, we observed difference between human and murine phagocytes in the uptake of C. parapsilosis. UV-treatment of fungal cells had varied effects on phagocytosis dependent upon which Candida strain was used. Besides statistical analysis, live cell imaging videos showed that this species similarly to the other two also has the ability to survive in host cells via the following mechanisms: yeast replication, and pseudohypha growth inside of phagocytes, exocytosis of fungal cells and also abortion of host cell mitosis following the uptake. According to our knowledge this is the first study that provides a thorough examination of C. parapsilosis phagocytosis and reports intracellular survival mechanisms associated with this species

Renata eToth

Adel eToth

Csaba ePapp

Ferenc eJankovic

Csaba eVagvolgyi

Judith Margaret Bain

Maria Fernanda Alonso

Lars Peter Erwig

Attila eGacser

Directory of Open Access Journals

Frontiers - Publisher Connector

Even though the number of Candida infections due to non-albicans species like C.  parapsilosis has been increasing, little is known about their pathomechanisms. Certain aspects of C. parapsilosis and host interactions have already been investigated; however we lack information about the innate cellular responses toward this species. The aim of our project was to dissect and compare the phagocytosis of C. parapsilosis to C. albicans and to another Candida species C.  glabrata by murine and human macrophages by live cell video microscopy. We broke  down the phagocytic process into three stages: macrophage migration, engulfment of fungal cells and host cell killing after the uptake. Our results showed increased macrophage migration toward C. parapsilosis and we observed differences during the engulfment processes when comparing the three species. The engulfment  time of C. parapsilosis was comparable to that of C. albicans regardless of the pseudohypha length and spatial orientation relative to phagocytes, while the rate of host cell killing and the overall uptake regarding C. parapsilosis showed similarities mainly with C. glabrata. Furthermore, we observed difference between human and murine phagocytes in the uptake of C. parapsilosis. UV-treatment of fungal cells had varied effects on phagocytosis dependent upon which Candida strain was used. Besides statistical analysis, live cell imaging videos showed that this species similarly to the other two also has the ability to survive in host cells via the following mechanisms: yeast replication, and pseudohypha growth inside of phagocytes, exocytosis of fungal cells and also abortion of host cell mitosis following the uptake. According to our knowledge this is the first study that provides a thorough examination of C. parapsilosis phagocytosis and reports intracellular survival mechanisms associated with this species

Tóth Renáta

Tóth Adél

Papp Csaba Gergő

Jankovics Ferenc

Vágvölgyi Csaba

Alonso Maria F.

Bain Judith M.

Erwig Lars-Peter

Gácser Attila

SZTE Publicatio Repozitórium - SZTE - Repository of Publications

Kinetic studies of Candida parapsilosis phagocytosis by macrophages and detection of intracellular survival mechanisms.

Aberdeen University Research

http://publicatio.bibl.u-szeged.hu/7556/6/fmicb-05-00633.pdf

Kinetic studies of Candida parapsilosis phagocytosis by macrophages and detection of intracellular survival mechanisms

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Directory of Open Access Journals

Frontiers - Publisher Connector

Frontiers - Publisher Connector

SZTE Publicatio Repozitórium - SZTE - Repository of Publications

Aberdeen University Research