Duramycin-induced calcium release in cancer cells

Introduction: Duramycin through binding with phosphatidylethanolamine (PE) has shown potential to be an effective anti-tumour agent. However its mode of action in relation to tumour cells is not fully understood. Methods: PE expression on the surface of a panel of cancer cell lines was analysed using duramycin and subsequent antibody labelling then analysed by flow cytometry. Cell viability was also assessed via flow cytometry using annexin V and propidium iodide (PI). Calcium ion (Ca²⁺) release by tumour cells in response to duramycin was determined by spectrofluorometry following incubation with Fluo-3, AM. Confocal microscopy was performed on the cancer cell line AsPC-1 to assess real time cell response to duramycin treatment. Results: Duramycin was able to detect cell surface PE expression on all 15 cancer cell lines screened, which was shown to be duramycin concentration dependent. However higher concentrations induced necrotic cell death. Duramycin induced calcium ion (Ca²⁺) release from the cancer cell lines also in a concentration and time dependent manner. Confocal microscopy showed an influx of PI into the cells over time and induced morphological changes. Conclusion: Duramycin induces Ca²⁺ release from cancer cell lines in a time and concentration dependent relationship

PARAS program: Phased array radio astronomy from space

An orbiting radio telescope is proposed which, when operated in a Very Long Baseline Interferometry (VLBLI) scheme, would allow higher (than currently available) angular resolution and dynamic range in the maps, and the ability of observing rapidly changing astronomical sources. Using a passive phases array technology, the proposed design consists of 656 hexagonal modules forming a 150 meter diameter dish. Each observatory module is largely autonomous, having its own photovoltaic power supply and low-noise receiver and processor for phase shifting. The signals received by the modules are channeled via fiber optics to the central control computer in the central bus module. After processing and multiplexing, the data is transmitted to telemetry stations on the ground. The truss frame supporting each observatory pane is a hybrid structure consisting of a bottom graphite/epoxy tubular triangle and rigidized inflatable Kevlar tubes connecting the top observatory panel and bottom triangle. Attitude control and stationkeeping functions are performed by a system of momentum wheels in the bus and four propulsion modules located at the compass points on the periphery of the observatory dish. Each propulsion module has four monopropellant thrusters and six hydrazine arcjets, the latter supported by a nuclear reactor. The total mass of the spacecraft is 22,060 kg

Metaverse Utilization in the EFL Classroom - A Study. Second Life (Register Trademark) as Part of an Institution's Curriculum

Starting on April 2010, the Kanda Institute of Foreign Languages integrated the Metaverse application known as Second Life into the core curriculum for 840 new enrollments. As of April 2011, we enrolled an additional 760 students. This course, the Hybrid English Learning Program, is commonly referred to by the acronym: HELP. By taking the advantages of learning in immersive 3D environments, we aim to meet two objectives, 1) namely to enable students to remember and properly use learned phrases and expressions, and 2) using Second Life(R) as a tool to enhance the meaning and practicality of what is being learned. ,Although there have been several online English learning programs or services here in Japan, this endeavor represents the first time Metaverse technology has been utilized as part of a core curriculum; a curriculum structured upon classroom learning and interaction within the Metaverse. This paper endeavors to explain the following: the planning and development of HELP, the class structure, and the results of a questionnaire which measured whether using such an approach would reduce student timidity and assist student to gain more confidence in speaking English in different social environments

Large-scale variation in density of an aquatic ecosystem indicator species

Funding: This work was supported by the New York State Department of Environmental Conservation and the Hudson River Natural Resource Trustees.Monitoring indicator species is a pragmatic approach to natural resource assessments, especially when the link between the indicator species and ecosystem state is well justified. However, conducting ecosystem assessments over representative spatial scales that are insensitive to local heterogeneity is challenging. We examine the link between polychlorinated biphenyl (PCB) contamination and population density of an aquatic habitat specialist over a large spatial scale using non-invasive genetic spatial capture-recapture. Using American mink (Neovison vison), a predatory mammal and an indicator of aquatic ecosystems, we compared estimates of density in two major river systems, one with extremely high levels of PCB contamination (Hudson River), and a hydrologically independent river with lower PCB levels (Mohawk River). Our work supports the hypothesis that mink densities are substantially (1.64-1.67 times) lower in the contaminated river system. We demonstrate the value of coupling the indicator species concept with well-conceived and spatially representative monitoring protocols. PCBs have demonstrable detrimental effects on aquatic ecosystems, including mink, and these effects are likely to be profound and long-lasting, manifesting as population-level impacts. Through integrating non-invasive data collection, genetic analysis, and spatial capture-recapture methods, we present a monitoring framework for generating robust density estimates across large spatial scales.Publisher PDFPeer reviewe

Eutrofización costera en la Península de Yucatán

28 páginas, 12 figuras, 2 tablas.-- Artículo incluido en el Cap.IV: Manejo de la Zona Costera del Golfo de México.El financiamiento ha provenido del CONACYT (PO20COOR; 4147PT; 32356T; SISIERRA-2000-07-06-15), CONABIO (B019; M011; S004) y CINVESTAV-IPN Unidad Merida.Peer reviewe

An imaging neutron/gamma-ray spectrometer

We present the design and development of a dual-species, neutron/γ-ray imaging spectrometer for the identification and location of radioactive and special nuclear materials (SNM). Real-time detection and identification is important for locating fissile materials. These materials, specifically uranium and plutonium, emit neutrons and γ rays via spontaneous or induced fission. Co-located neutron and γ-ray emissions are a sure sign of fissile material, requiring very few spatially correlated events for a significant detection. Our instrument design detects neutrons and γ rays from all sources in its field of view, constructs images of the emission pattern, and reports the spectra for both species. The detection principle is based upon multiple elastic neutron-proton scatters in organic scintillator for neutrons, and Compton scattering in organic scintillator followed by photoelectric absorption in inorganic scintillator for γ rays. The instrument is optimized for neutron imaging and spectroscopy in the 1-20 MeV range. We recorded images and spectra of a Cf-252 source from 0.5 - 10 MeV, and have done similarly for several γ-ray sources. We report the results of laboratory testing of this expanded instrument and compare them to detailed Monte Carlo simulations using Geant4

Detecting the Most Distant (z>7) Objects with ALMA

Detecting and studying objects at the highest redshifts, out to the end of Cosmic Reionization at z>7, is clearly a key science goal of ALMA. ALMA will in principle be able to detect objects in this redshift range both from high-J (J>7) CO transitions and emission from ionized carbon, [CII], which is one of the main cooling lines of the ISM. ALMA will even be able to resolve this emission for individual targets, which will be one of the few ways to determine dynamical masses for systems in the Epoch of Reionization. We discuss some of the current problems regarding the detection and characterization of objects at high redshifts and how ALMA will eliminate most (but not all) of them.Comment: to appear in Astrophysics and Space Science, "Science with ALMA: a new era for Astrophysics", ed. R. Bachille

Development of a framework for the co-production and prototyping of public health interventions

BACKGROUND: Existing guidance for developing public health interventions does not provide information for researchers about how to work with intervention providers to co-produce and prototype the content and delivery of new interventions prior to evaluation. The ASSIST + Frank study aimed to adapt an existing effective peer-led smoking prevention intervention (ASSIST), integrating new content from the UK drug education resource Talk to Frank ( www.talktofrank.com ) to co-produce two new school-based peer-led drug prevention interventions. A three-stage framework was tested to adapt and develop intervention content and delivery methods in collaboration with key stakeholders to facilitate implementation. METHODS: The three stages of the framework were: 1) Evidence review and stakeholder consultation; 2) Co-production; 3) Prototyping. During stage 1, six focus groups, 12 consultations, five interviews, and nine observations of intervention delivery were conducted with key stakeholders (e.g. Public Health Wales [PHW] ASSIST delivery team, teachers, school students, health professionals). During stage 2, an intervention development group consisting of members of the research team and the PHW ASSIST delivery team was established to adapt existing, and co-produce new, intervention activities. In stage 3, intervention training and content were iteratively prototyped using process data on fidelity and acceptability to key stakeholders. Stages 2 and 3 took the form of an action-research process involving a series of face-to-face meetings, email exchanges, observations, and training sessions. RESULTS: Utilising the three-stage framework, we co-produced and tested intervention content and delivery methods for the two interventions over a period of 18 months involving external partners. New and adapted intervention activities, as well as refinements in content, the format of delivery, timing and sequencing of activities, and training manuals resulted from this process. The involvement of intervention delivery staff, participants and teachers shaped the content and format of the interventions, as well as supporting rapid prototyping in context at the final stage. CONCLUSIONS: This three-stage framework extends current guidance on intervention development by providing step-by-step instructions for co-producing and prototyping an intervention's content and delivery processes prior to piloting and formal evaluation. This framework enhances existing guidance and could be transferred to co-produce and prototype other public health interventions. TRIAL REGISTRATION: ISRCTN14415936 , registered retrospectively on 05 November 2014

Role of Cerebral Blood Flow in Extreme Breath Holding

The role of cerebral blood flow (CBF) on a maximal breath-hold (BH) in ultra-elite divers was examined. Divers (n = 7) performed one control BH, and one BH following oral administration of the non-selective cyclooxygenase inhibitor indomethacin (1.2 mg/kg). Arterial blood gases and CBF were measured prior to (baseline), and at BH termination. Compared to control, indomethacin reduced baseline CBF and cerebral delivery of oxygen (CDO(2)) by about 26% (p < 0.01). Indomethacin reduced maximal BH time from 339 ± 51 to 319 ± 57 seconds (p = 0.04). In both conditions, the CDO(2) remained unchanged from baseline to the termination of apnea. At BH termination, arterial oxygen tension was higher following oral administration of indomethacin compared to control (4.05 ± 0.45 vs. 3.44 ± 0.32 kPa). The absolute increase in CBF from baseline to the termination of apnea was lower with indomethacin (p = 0.01). These findings indicate that the impact of CBF on maximal BH time is likely attributable to its influence on cerebral H(+) washout, and therefore central chemoreceptive drive to breathe, rather than to CDO(2)