Life History Trade-offs Between Growth and Reproduction in Wild Yeast

Life history trade-offs are an organism\u27s balancing of energy allocations to necessary strategies of life: growth, reproduction, and survival. The success of a life history strategy depends on both the environment and the developmental constraints of an organism. The yeast Saccharomyces cerevisiae is an ideal organism to investigate microbial life history trade-offs. Since these yeast are found in a variety of environments with niche-specific strategies and the S. cerevisiae life cycle involves both sexual and asexual phases, I expected to see a life history trade-off between growth and reproduction within and between populations of yeast. A strain more efficient at mating should be a slower grower while a slow mater should proceed through the cell cycle quicker. To study this trade-off, I measured the relative rates of mating and asexual growth. I found a significant difference in both the speed of sexual and asexual reproduction within a single population as well as between populations from different environments. The results presented here are still preliminary, as the assays used to measure the relevant life history traits were prone to experimental error. Given that caveat, within the woodland population of yeast, which represents three distinct haplotypes, there appears to be an inverse relationship between the length of the cell cycle and the rate of mating, indicating a trade-off between the efficiency of each strategy. No pattern was found in strains of other ecotypes

First Steps Toward Change in Teacher Preparation for Elementary Science

Unless introductory undergraduate science classes for prospective elementary teachers actively incorporate the philosophy of inquiry-based learning called for in K-l2 science education refom little will change in elementary science education. Thus, at James Madison University, we have developed a new integrated science core curriculum called Understanding our World [1]. This course sequence was not only designed to fulfill general education science requirements. but also to focus on content areas our students will need to know as teachers. The objectives of these courses are based on the National Science Education Standards and Virginia’s Science Standards of Learning, including earth and space science, chemistry, physics, life sciences, and environmental science [2,3]. As an integrated package, this course sequence addresses basic science content, calculation skills, the philosophy and history of science, the process of how science is done, the role of science in society, and applications of computers and technology in science. Keeping in mind that students tend to teach in the same way they were taught, Understanding our World core classes embrace the concepts associated with reform in elementary math and science

Skin-friction generation by attached eddies in turbulent channel flow

Despite a growing body of recent evidence on the hierarchical organization of the selfsimilar energy-containing motions in the form of Townsend’s attached eddies in wallbounded turbulent flows, their role in turbulent skin-friction generation is currently known very little. In this paper, the contribution of each of these self-similar energycontaining motions to turbulent skin friction is explored up to Reτ ≃ 4000. Three different approaches are employed to quantify the skin-friction generation by the motions, the spanwise length scale of which is smaller than a given cut-off wavelength: 1) FIK identity in combination with the spanwise wavenumber spectra of the Reynolds shear stress; 2) confinement of the spanwise computational domain; 3) artificial damping of the motions to be examined. The near-wall motions are found to continuously lose their role in skin-friction generation on increasing the Reynolds number, consistent with the previous finding at low Reynolds numbers. The largest structures given in the form of very-large-scale and large-scale motions are also found to be of limited importance: due to a non-trivial scale-interaction process, their complete removal yields only 5 ∼ 8% of skin-friction reduction at all the Reynolds numbers considered, although they are found to be responsible for 20 ∼ 30% of total skin friction at Reτ ≃ 2000. Application of all the three approaches consistently reveals that the largest amount of skin friction is generated by the self-similar motions populating the logarithmic region. It is further shown that the contribution of these motions to turbulent skin friction gradually increases with the Reynolds number, and that these coherent structures are eventually responsible for most of turbulent skin-friction generation at sufficiently high Reynolds numbers

Streak instability in turbulent channel flow: the seeding mechanism of large-scale motions

It has often been proposed that the formation of large-scale motion (or bulges) is a consequence of successive mergers and/or growth of near-wall hairpin vortices. In the present study, we report our direct observation that large-scale motion is generated by an instability of an ‘amplified’ streaky motion in the outer region (i.e. very-large-scale motion). We design a numerical experiment in turbulent channel flow up to Reτ '2000 where a streamwise-uniform streaky motion is artificially driven by body forcing in the outer region computed from the previous linear theory (Hwang & Cossu, J. Fluid Mech., vol. 664, 2015, pp. 51–73). As the forcing amplitude is increased, it is found that an energetic streamwise vortical structure emerges at a streamwise wavelength of λx/h '1–5 (h is the half-height of the channel). The application of dynamic mode decomposition and the examination of turbulence statistics reveal that this structure is a consequence of the sinuous-mode instability of the streak, a subprocess of the self-sustaining mechanism of the large-scale outer structures. It is also found that the statistical features of the vortical structure are remarkably similar to those of the largescale motion in the outer region. Finally, it is proposed that the largest streamwise length of the streak instability determines the streamwise length scale of very-largescale motion

Streak instability in near-wall turbulence revisited

The regeneration cycle of streaks and streamwise vortices plays a central role in the sustainment of near-wall turbulence. In particular, the streak breakdown phase in the regeneration cycle is the core process in the formation of the streamwise vortices, but its current understanding is limited particularly in a real turbulent environment. This study is aimed at gaining fundamental insight into the underlying physical mechanism of the streak breakdown in the presence of background turbulent fluctuation. We perform a numerical experiment based on direct numerical simulation, in which streaks are artificially generated by a body forcing computed from previous linear theory. Upon increasing the forcing amplitude, the artificially driven streaks are found to generate an intense fluctuation of the wall-normal and spanwise velocities in a fairly large range of amplitudes. This cross-streamwise velocity fluctuation shows its maximum at λ+ x ≈ 200 − 300 (λ+ x is the inner-scaled streamwise wavelength), but it only appears for λ+ x ≲ 3000 − 4000. Further examination with dynamic mode decomposition reveals that the related flow field is composed of sinuous meandering motion of the driven streaks and alternating cross-streamwise velocity structures, clearly reminiscent of sinuous-mode streak instability found in previous studies. Finally, it is shown that these structures are reasonably well aligned along the critical layer of the secondary instability, indicating that the surrounding turbulence does not significantly modify the inviscid inflectional mechanism of the streak breakdown via streak instability and/or streak transient growth

Prophage association of mef(A) elements encoding efflux-mediated erythromycin resistance in Streptococcus pyogenes.

OBJECTIVES: To compare different mef(A) elements of Streptococcus pyogenes for a possible chimeric genetic nature, i.e. a transposon inserted into a prophage. METHODS: Eleven S. pyogenes isolates with efflux-mediated erythromycin resistance were used. The isolates were typed using several genotypic approaches. Gene detection was performed by PCR using specific primer pairs. The mef(A) elements of the test strains were induced with mitomycin C and phage DNA was extracted. Induction was monitored by PCR using primers targeting mef(A). RESULTS: Six tetracycline-susceptible isolates had PCR evidence of all of the eight open reading frames (ORFs) of the Tn1207.1 element; their mef(A) element was consistent with the Tn1207.3 element in four isolates and with the 58.8 kb chimeric element in two. Five tetracycline-resistant isolates had no PCR evidence of orf1 and orf2 and showed variable patterns as to orf3, orf7, and orf8. Three ORFs placed along the conserved region downstream of Tn1207.1 in the 58.8 kb mef(A) chimeric element were detected in the six tetracycline-susceptible, but not in the five tetracycline-resistant isolates. Induction assays with mitomycin C demonstrated that the mef(A) elements of all strains tested were present in culture supernatants in a DNAse-resistant form, such as a phage capsid. CONCLUSIONS: All recognized mef(A) elements of S. pyogenes appear to be prophage-associated. Whereas the two elements detected in tetracycline-susceptible isolates (Tn1207.3 and the 58.8 kb one) were apparently inserted into the same prophage, the tet(O)-mef(A) element was inserted into a different prophage. Phage transfer is likely to play a critical role in the dissemination of erythromycin resistance in S. pyogenes populations

Acorn isotopic composition: A new promising tool for authenticity maps of Montado’s high-value food products

It is often overlooked that even food production is linked to the ecology of plants and animals. Living organisms respond to environmental short-and long-term variability: acknowledging this may help in the ultimate goal of valorizing a territory/product. We investigated acorns of the two main Quercus species of the Portuguese Montado, a main feed of the renown black Iberian pig. We tested their responses to an aridity gradient by morphological parameters and isotopic signature. Q. rotundifolia and Q. suber acorns did not differ morphologically, even if a higher variability in all parameters was observed in acorns of Q. suber. According to the site-specific Aridity Index, correlations are indicative to higher weight and length only in Q. suber acorns from more arid sites. As for isotopic composition, there were no differences in nitrogen or carbon (δ15N and δ13C) between the two species. However, combining the samples and testing for association with the Aridity Index, we found that more arid sites lead to a 15N enrichment. This result, combined with the positive correlation between AI and acorns length, support the use of acorns as a tool, their isoscapes of nitrogen being a stepping stone for the provenance of the black Iberian pig.info:eu-repo/semantics/publishedVersio

Biodiversity evaluation: From endorsed indexes to inclusion of a pollinator indicator

There is increasing interest in evaluating biodiversity to preserve ecosystem services. Researchers can sustain policymakers by providing tools, such as indexes and indicators, that need constant implementation to become accepted standards. Implementation may vary from re-evaluation of existing indicators to introduction of new ones based on emerging threats to biodiversity. With the aim of contributing to the compelling need to estimate and counterbalance pollinator loss, we screened existing bioindicators. We first selected indexes/indicators applied to agricultural contexts and concurrently endorsed by a regulatory agency. We then extended our analysis to indexes/indicators based on arthropod taxa and formally recognized at least by national bodies. Our procedure identified a combination of surveys of various animal taxa and remote landscape analyses (e.g., using a GIS and other cartographic tools). When the animals are arthropods, most indexes/indicators can only address confined environments (e.g., grasslands, riversides). Indicator strength was improved by the simultaneous inclusion of biotic and abiotic components. Pollinator sensitivity to changes at micro-habitat level is widely appreciated and may help distinguish agricultural practices. A biodiversity index based on pollinators, including a wide monitoring scheme supplemented by citizen science, is currently fostered at the European level. The results obtained using such an index may finally enable focusing of strategic funding. Our analysis will help to reach this goal

Tools to tie: Flower characteristics, voc emission profile, and glandular trichomes of two mexican salvia species to attract bees

A plant can combine physical and chemical tools to interact with other organisms. Some are designed for pollinator attraction (i.e., colors and volatile organic compounds-VOCs); others can act to discourage herbivores (i.e., non-glandular trichomes). Few studies fully address available tools in a single species; notwithstanding, this information can be pivotal in understanding new interactions out of the home range. We characterized flower traits, emission profiles of constitutive compounds from flowers and leaves, micro-morphology of the glandular trichomes, and listed flower visitors of two Mexican bird-pollinated Salvia species (S. blepharophylla and S. greggii), growing in an Italian botanical garden. Flowers were highly variable in their morphometric characteristics. In both species, four trichome morphotypes with similar histochemistry and distribution were documented for leaves and flowers except the calyx abaxial side. The vegetative emission profiles were qualitatively more complex than the floral ones; however, common compounds occurring in high relative percentages were β-caryophyllene and germacrene D. Floral bouquets were dominated by limonene and β-pinene in S. greggii and by 1,8-cineole in S. blepharophylla. Two potential (non-bird) pollinators were especially abundant: small bees belonging to the genus Lasioglossum and large bees belonging to the species Xylocopa violacea. Our study highlights the plasticity of these plants, as well as tools that can be conveniently used to establish novel interactions