189 research outputs found
Fluorescent biosensors based on silicon nanowires
Nanostructures are arising as novel biosensing platforms promising to surpass current performance in terms of sensitivity, selectivity, and affordability of standard approaches. However, for several nanosensors, the material and synthesis used make the industrial transfer of such technologies complex. Silicon nanowires (NWs) are compatible with Si-based flat architecture fabrication and arise as a hopeful solution to couple their interesting physical properties and surface-to-volume ratio to an easy commercial transfer. Among all the transduction methods, fluorescent probes and sensors emerge as some of the most used approaches thanks to their easy data interpretation, measure affordability, and real-time in situ analysis. In fluorescent sensors, Si NWs are employed as substrate and coupled with several fluorophores, NWs can be used as quenchers in stem-loop configuration, and have recently been used for direct fluorescent sensing. In this review, an overview on fluorescent sensors based on Si NWs is presented, analyzing the literature of the field and highlighting the advantages and drawbacks for each strategy
Dust properties of Lyman break galaxies at
We explore from a statistical point of view the far-infrared (far-IR) and
sub-millimeter (sub-mm) properties of a large sample of LBGs (22,000) at z~3 in
the COSMOS field. The large number of galaxies allows us to split it in several
bins as a function of UV luminosity, UV slope, and stellar mass to better
sample their variety. We perform stacking analysis in PACS (100 and 160 um),
SPIRE (250, 350 and 500 um) and AzTEC (1.1 mm) images. Our stacking procedure
corrects the biases induced by galaxy clustering and incompleteness of our
input catalogue in dense regions. We obtain the full IR spectral energy
distributions (SED) of subsamples of LBGs and derive the mean IR luminosity as
a function of UV luminosity, UV slope, and stellar mass. The average IRX is
roughly constant over the UV luminosity range, with a mean of 7.9 (1.8 mag).
However, it is correlated with UV slope, and stellar mass. We investigate using
a statistically-controlled stacking analysis as a function of (stellar mass, UV
slope) the dispersion of the IRX-UVslope and IRX-M* plane. Our results enable
us to study the average relation between star-formation rate (SFR) and stellar
mass, and we show that our LBG sample lies on the main sequence of star
formation at z~3.Comment: Accepted to A&A, 17 Pages, 14 Figures, 2 Table
Lymphocytes from rheumatoid arthritis patients have elevated levels of intracellular peroxiredoxin 2, and a greater frequency of cells with exofacial peroxiredoxin 2, compared with healthy human lymphocytes
AbstractPeroxiredoxin 2 has immune regulatory functions, but its expression in human peripheral blood lymphocytes and levels in extracellular fluid in healthy subjects and rheumatoid arthritis patients are poorly described. In the present study, the median intracellular peroxiredoxin 2 protein content of lymphocytes from rheumatoid arthritis patients was more than two-fold higher compared with healthy subjectsâ lymphocytes. Intracellular peroxiredoxin 3 levels were similar in healthy and rheumatoid arthritis lymphocytes. Flow cytometry detected peroxiredoxin 2 on the surface of ca. 8% of T cells and ca. 56% of B cells (median % values) of all subjects analyzed. Exofacial thioredoxin-1 was also observed. In the total lymphocyte population from rheumatoid arthritis patients, few cells (median, 6%) displayed surface peroxiredoxin 2. In contrast, a significantly increased proportion of interleukin-17+ve lymphocytes were exofacially peroxiredoxin 2+ve (median, 39%). Prdx2 was also detected in human extracellular fluids. We suggest that crucial inflammatory cell subsets, i.e. interleukin-17+ve T cells, exhibit increased exofacial redox-regulating enzymes and that peroxiredoxin 2 may be involved in the persistence of pro-inflammatory cells in chronic inflammation
HerMES: the rest-frame UV emission and a lensing model for the z= 6.34 luminous dusty starburst galaxy HFLS3
We discuss the rest-frame ultraviolet emission from the starbursting galaxy HFLS3 at a redshift of 6.34. The galaxy was discovered in Herschel/SPIRE data due to its red color in the submillimeter wavelengths from 250 to 500 ÎŒm. Keck/NIRC2 K s -band adaptive optics imaging data showed two potential near-IR counterparts near HFLS3. Previously, the northern galaxy was taken to be in the foreground at z = 2.1, while the southern galaxy was assumed to be HFLS3's near-IR counterpart. The recently acquired Hubble/WFC3 and Advanced Camera for Surveys (ACS) imaging data show conclusively that both optically bright galaxies are in the foreground at z < 6. A new lensing model based on the Hubble imaging data and the millimeter-wave continuum emission yields a magnification factor of 2.2 ± 0.3, with a 95% confidence upper limit on the magnification of 3.5. When corrected for lensing, the instantaneous star formation rate is 1320 M â yrâ1, with the 95% confidence lower limit around 830 M â yrâ1. The dust and stellar masses of HFLS3 from the same spectral energy distribution (SED) models are at the level of 3 Ă 108 M â and ~5 Ă 1010 M â, respectively, with large systematic uncertainties on assumptions related to the SED model. With Hubble/WFC3 images, we also find diffuse near-IR emission about 0.5 arcsec (~3 kpc) to the southwest of HFLS3 that remains undetected in the ACS imaging data. The emission has a photometric redshift consistent with either z ~ 6 or a dusty galaxy template at z ~ 2
A quiescent galaxy at the position of the long GRB 050219A
Long-duration gamma-ray bursts (LGRBs) are produced by the collapse of very
massive stars. Due to the short lifetime of their progenitors, LGRBs pinpoint
star-forming galaxies. We present here a multi-band search for the host galaxy
of the long dark GRB 050219A within the enhanced Swift/XRT error circle. We
used spectroscopic observations acquired with VLT/X-shooter to determine the
redshift and star-formation rate of the putative host galaxy. We compared the
results with the optical/IR spectral energy distribution obtained with
different facilities. Surprisingly, the host galaxy is a old and quiescent
early-type galaxy at z = 0.211 characterised by an unprecedentedly low specific
star-formation rate. It is the first LGRB host to be also an early-type
post-starburst galaxy. This is further evidence that GRBs can explode in all
kind of galaxies, with the only requirement being an episode of star-formation.Comment: 11 pages, 10 figures, 3 tables; accepted for publication in Astronomy
& Astrophysic
Differences in clinical presentation of primary open-angle glaucoma between African and European populations
PURPOSE: Primary openâangle glaucoma (POAG) has been reported to occur more frequently in Africans, and to follow a more severe course compared to Europeans. We aimed to describe characteristics of POAG presentation and treatment across three ethnic groups from Africa and one from Europe. METHODS: We ascertained 151 POAG patients from South African Coloured (SAC) and 94 South African Black (SAB) ethnicity from a university hospital in South Africa. In Tanzania, 310 patients were recruited from a university hospital and a referral hospital. In the Netherlands, 241 patients of European ancestry were included. All patients were over 35 years old and had undergone an extensive ophthalmic examination. Patients were diagnosed according to the ISGEO criteria. A biogeographic ancestry analysis was performed to estimate the proportion of genetic African ancestry (GAA). RESULTS: The biogeographic ancestry analysis showed that the median proportion of GAA was 97.6% in Tanzanian, 100% in SAB, 34.2% in SAC and 1.5% in Dutch participants. Clinical characteristics at presentation for Tanzanians, SAB, SAC and Dutch participants, respectively: mean age: 63, 57, 66, 70 years (p < 0.001); visual acuity in the worse eye: 1.78, 1.78, 0.3, 0.3 LogMAR (p < 0.001); maximum intraocular pressure of both eyes: 36, 34, 29, 29 mmHg (p (anova)  < 0.001); maximum vertical cup to disc ratio (VCDR) of both eyes: 0.90, 0.90, 0.84, 0.83 (p < 0.001); mean central corneal thickness: 506, 487, 511, 528 Όm (p < 0.001). Fourteen percent of Tanzanian patients presented with blindness (<3/60 Snellen) in the better eye in contrast to only 1% in the Dutch. CONCLUSION: In this multiâethnic comparative study, SubâSaharan Africans present at a younger age with lower visual acuity, higher IOP, larger VCDR, than SAC and Dutch participants. This indicates the more progressive and destructive course in SubâSaharan Africans
Environment of the submillimeter-bright massive starburst HFLS3 at 6.34
We describe the search for Lyman-break galaxies (LBGs) near the
sub-millimeter bright starburst galaxy HFLS3 at 6.34 and a study on the
environment of this massive galaxy during the end of reionization.We performed
two independent selections of LBGs on images obtained with the \textit{Gran
Telescopio Canarias} (GTC) and the \textit{Hubble Space Telescope} (HST) by
combining non-detections in bands blueward of the Lyman-break and color
selection. A total of 10 objects fulfilling the LBG selection criteria at
5.5 were selected over the 4.54 and 55.5 arcmin covered by our HST
and GTC images, respectively. The photometric redshift, UV luminosity, and the
star-formation rate of these sources were estimated with models of their
spectral energy distribution. These 6 candidates have physical
properties and number densities in agreement with previous results. The UV
luminosity function at 6 and a Voronoi tessellation analysis of this
field shows no strong evidence for an overdensity of relatively bright objects
(m25.9) associated with \textit{HFLS3}. However, the over-density
parameter deduced from this field and the surface density of objects can not
excluded definitively the LBG over-density hypothesis. Moreover we identified
three faint objects at less than three arcseconds from \textit{HFLS3} with
color consistent with those expected for 6 galaxies. Deeper data are
needed to confirm their redshifts and to study their association with
\textit{HFLS3} and the galaxy merger that may be responsible for the massive
starburst.Comment: 14 pages, 12 figures, accepted for publication in Ap
The fraction of early-type galaxies in low redshift groups and clusters of galaxies
We examine the fraction of early-type (and spiral) galaxies found in groups
and clusters of galaxies as a function of dark matter halo mass. We use
morphological classifications from the Galaxy Zoo project matched to halo
masses from both the C4 cluster catalogue and the Yang et al (2007) group
catalogue. We find that the fraction of early-type (or spiral) galaxies remains
constant (changing by less than 10%) over three orders of magnitude in halo
mass (13<log MH/Msol/h<15.8). This result is insensitive to our choice of halo
mass measure, from velocity dispersions or summed optical luminosity.
Furthermore, we consider the morphology-halo mass relations in bins of galaxy
stellar mass M*, and find that while the trend of constant fraction remains
unchanged, the early-type fraction amongst the most massive galaxies (11<log
M*/Msol/h <12) is a factor of three greater than lower mass galaxies
(10<logM*/Msol/h<10.7). We compare our observational results with those of
simulations presented in De Lucia et al (2011), as well as previous
observational analyses, and semi-analytic bulge (or disc) dominated galaxies
from the Millennium Simulation. We find the simulations recover similar trends
as observed, but may over-predict the abundances of the most massive bulge
dominated (early-type) galaxies. Our results suggest that most morphological
transformation is happening on the group scale before groups merge into massive
clusters. However, we show that within each halo a morphology-density relation
remains: it is summing the total fraction to a self-similar scaled radius which
results in a flat morphology-halo mass relationship.Comment: 9 page, 5 figures, modified to match accepted version (MNRAS
Mesenchymal stromal cell treatment of donor kidneys during ex-vivo normothermic machine perfusion:A porcine renal autotransplantation study
Normothermic machine perfusion (NMP) of injured kidneys offers the opportunity for interventions to metabolically active organs prior to transplantation. Mesenchymal stromal cells (MSCs) can exert regenerative and anti-inflammatory effects in ischaemia-reperfusion injury. The aims of this study were to evaluate the safety and feasibility of MSC treatment of kidneys during NMP using a porcine auto-transplantation model, and examine potential MSC treatment-associated kidney improvements up to 14 days post transplantation. After 75 minutes of kidney warm ischaemia, four experimental groups of n=7 underwent 14 hours of oxygenated hypothermic machine perfusion. In three groups this was followed by 240 minutes of NMP with infusion of vehicle, ten million porcine or ten million human adipose derived MSCs. All kidneys were auto-transplanted after contralateral nephrectomy. MSC treatment did not affect perfusion haemodynamics during NMP or cause adverse effects at reperfusion, with 100% animal survival. MSCs did not affect plasma creatinine, glomerular filtration rate, neutrophil gelatinase-associated lipocalin concentrations or kidney damage assessed by histology during the 14 days, and MSCs retention was demonstrated in renal cortex. Infusing MSCs during ex vivo NMP of porcine kidneys was safe and feasible. Within the short post- transplant
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