A laboratory based experimental study of mercury emission from contaminated soils in the River Idrijca catchment

Results obtained by a laboratory flux measurement system (LFMS) focused on investigating the kinetics of the mercury emission flux (MEF) from contaminated soils of the Idrija Hg-mine region, Slovenia are presented. Representative soil samples with respect to total Hg concentrations (4–417 μg g<sup>−1</sup>) and land cover (forest, meadow and alluvial soil) alongside the River Idrijca were analysed to determine the variation in MEF versus distance from the source, regulating three major environmental parameters comprising soil temperature, soil moisture and solar radiation. MEFs ranged from less than 2 to 530 ng m<sup>−2</sup> h<sup>−1</sup>, with the highest emissions from contaminated alluvial soils and soils near the mining district in the town of Idrija. A significant decrease of MEF was then observed with increasing distance from these sites. The results revealed a strong positive effect of all three parameters investigated on momentum MEF. The light-induced flux was shown to be independent of the soil temperature, while the soil aqueous phase seems to be responsible for recharging the pool of mercury in the soil available for both the light- and thermally-induced flux. The overall flux response to simulated environmental conditions depends greatly on the form of Hg in the soil. Higher activation energies are required for the overall process to occur in soils where insoluble cinnabar prevails compared to soils where more mobile Hg forms and forms available for transformation processes are dominant

Investigating the process of ethical approval in citizen science research. The case of public health

Undertaking citizen science research in Public Health involving human subjects poses significant challenges concerning the traditional process of ethical approval. It requires an extension of the ethics of protection of research subjects in order to include the empowerment of citizens as citizen scientists. This paper investigates these challenges and illustrates the ethical framework and the strategies developed within the CitieS-Health project. It also proposes first recommendations generated from the experiences of five citizen science pilot studies in environmental epidemiology within this project

Homogeneity and persistence of transgene expression by omitting antibiotic selection in cell line isolation

Nonuniform, mosaic expression patterns of transgenes are often linked to transcriptional silencing, triggered by epigenetic modifications of the exogenous DNA. Such phenotypes are common phenomena in genetically engineered cells and organisms. They are widely attributed to features of transgenic transcription units distinct from endogenous genes, rendering them particularly susceptible to epigenetic downregulation. Contrary to this assumption we show that the method used for the isolation of stably transfected cells has the most profound impact on transgene expression patterns. Standard antibiotic selection was directly compared to cell sorting for the establishment of stable cells. Only the latter procedure could warrant a high degree of uniformity and stability in gene expression. Marker genes useful for the essential cell sorting step encode mostly fluorescent proteins. However, by combining this approach with site-specific recombination, it can be applied to isolate stable cell lines with the desired expression characteristics for any gene of interest

Assessing external exposome by implementing an Environmental Data Management System using Open Data

Due to the increasing importance of exposome in environmental epidemiology, feasibility and usefulness of an Environmental Data Management System (EDMS) using Open Data was evaluated. The EDMS includes data from 10 European cities (Celje (Slovenia), Łódź (Poland), Manchester (UK), Palermo (Italy), Paris (France), Porto (Portugal), Regensburg (Germany), Reus (Spain), Rijeka (Croatia), Thessaloniki (Greece)) about external non-specific and specific exposome factors at the city or country level (2017-2020). Findings showed that the highest values of life expectancy were in Reus females (86 years) and Palermo males (81 years). UK had the highest obesity rate (28%), Croatia the highest prescribed drug consumption (62%), Greece and Portugal the highest smoking rates (37%, 42%) and daily alcohol consumption (21%), respectively. The most polluted cities were Thessaloniki for PM10 (38 µg/m3), Łódź for PM2.5 (25 µg/m3), Porto for NO2 (62 µg/m3) and Rijeka for O3 (92 µg/m3). Thessaloniki had the highest grey space (98%) and Łódź the highest cumulative amount of pollen (39,041 p/m3). The highest daily noise levels ≥ 55 dB was in Reus (81% to traffic) and Regensburg (21% to railway). In drinking water, arsenic had the highest value in Thessaloniki (6.4 µg/L), boron in Celje (24 mg/L) and lead in Paris (46.7 µg/L). Portugal and Greece showed the highest pesticide residues in food (7%). In conclusion, utilizing open-access databases enables the translation of research findings into actionable strategies for public health interventions.This work was supported by the European Commission and the Ministry of Education, Universities and Research, grant agreement IDs: 603946 (“Health and Environment-wide Associations based on Large population Surveys-HEALS”, European Union’s Seventh Framework Programme for Research, Technological Development and Demonstration Activities) and 696300 (“Long-term impact of gestational and early-life dietary habits on infant gut immunity and disease risk-EarlyFOOD”, European Union’s Joint Programming Initiative “A Healthy Diet for a Healthy Life” EHDHL-INTIMIC Cofunded Call "Interrelation of the Intestinal Microbiome, Diet and Health"). We thank Prof. M. Chałubiński and Dr. B. Majkowska-Wojciechowska from the Medical University of Lodz, Poland, and Dr. Jordina Belmonte Soler and the site of the Xarxa Aerobiològica de Catalunya in Tarragona (Spain) for the pollen data supply, Prof. Denis A. Sarigiannis for the HEALS project co-coordination. We acknowledge Prof. Michael Kabesh for the support during the HEALS EXHES Survey. We thank each local authority for the data supply, as well

Magnetic moments of short-lived nuclei with part-per-million accuracy: Towards novel applications of β\beta-detected NMR in physics, chemistry and biology

We determine for the first time the magnetic dipole moment of a short-lived nucleus with part-per-million (ppm) accuracy. To achieve this two orders of magnitude improvement over previous studies, we implement a number of innovations into our $\beta$-detected Nuclear Magnetic Resonance ($\beta$-NMR) setup at ISOLDE/CERN. Using liquid samples as hosts we obtain narrow, sub-kHz linewidth, resonances, while a simultaneous in-situ $^1$H NMR measurement allows us to calibrate and stabilize the magnetic field to ppm precision, thus eliminating the need for additional $\beta$-NMR reference measurements. Furthermore, we use ab initio calculations of NMR shielding constants to improve the accuracy of the reference magnetic moment, thus removing a large systematic error. We demonstrate the potential of this combined approach with the 1.1 s half-life radioactive nucleus $^{26}$Na, which is relevant for biochemical studies. Our technique can be readily extended to other isotopic chains, providing accurate magnetic moments for many short-lived nuclei. Furthermore, we discuss how our approach can open the path towards a wide range of applications of the ultra-sensitive $\beta$-NMR in physics, chemistry, and biology.Comment: re-submitte

An evaluation tool kit of air quality micro-sensing units.

Recent developments in sensory and communication technologies have made the development of portable air-quality (AQ) micro-sensing units (MSUs) feasible. These MSUs allow AQ measurements in many new applications, such as ambulatory exposure analyses and citizen science. Typically, the performance of these devices is assessed using the mean error or correlation coefficients with respect to a laboratory equipment. However, these criteria do not represent how such sensors perform outside of laboratory conditions in large-scale field applications, and do not cover all aspects of possible differences in performance between the sensor-based and standardized equipment, or changes in performance over time. This paper presents a comprehensive Sensor Evaluation Toolbox (SET) for evaluating AQ MSUs by a range of criteria, to better assess their performance in varied applications and environments. Within the SET are included four new schemes for evaluating sensors' capability to: locate pollution sources; represent the pollution level on a coarse scale; capture the high temporal variability of the observed pollutant and their reliability. Each of the evaluation criteria allows for assessing sensors' performance in a different way, together constituting a holistic evaluation of the suitability and usability of the sensors in a wide range of applications. Application of the SET on measurements acquired by 25 MSUs deployed in eight cities across Europe showed that the suggested schemes facilitates a comprehensive cross platform analysis that can be used to determine and compare the sensors' performance. The SET was implemented in R and the code is available on the first author's website.CITI-SENSE, initiated in October 2012, is a four year Collaborative Project partly funded by the EU FP7-ENV-2012 under grant agreement 308524

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

Background Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide.Methods A multimethods analysis was performed as part of the GlobalSurg 3 study-a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital.Findings Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3.85 [95% CI 2.58-5.75]; p<0.0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63.0% vs 82.7%; OR 0.35 [0.23-0.53]; p<0.0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer.Interpretation Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised

Ecological strategy for soil contaminated with mercury

Aims The paper presents results from plot experiments aimed at the development of an ecological strategy for soil contaminated with mercury. Meadow grass (Poa pratensis) was tested on mercury contaminated soil in a former chlor-alkali plant (CAP) in southern Poland for its phytoremediation potential. Methods The stabilisation potential of the plants was investigated on plots without additives and after the addition of granular sulphur. Biomass production, uptake and distribution of mercury by plants, as well as leachates and rhizosphere microorganisms were investigated, along with the growth and vitality of plants during one growing season. Results The analysed plants grew easily on mercury contaminated soil, accumulating lower amounts of mercury, especially in the roots, from soil with additive of granular sulphur (0.5 % w/w) and sustained a rich microbial population in the rhizosphere. After amendment application the reduction of Hg evaporation was observed. Conclusions The obtained results demonstrate the potential of using Poa pratensis and sulphur for remediation of mercury contaminated soil and reduction of the Hg evaporation from soil. In the presented study, methods of Hg reduction on “hot spots” were proposed, with a special focus on environmental protection. This approach provides a simple remediation tool for large areas heavily contaminated with mercury

Social Marketing Workshop Evaluation Report

This document reports upon two afternoon workshops on ‘Evaluating Social Marketing’, which were conducted on the 9th and 10th September 2009 for Eastern and Coastal Kent (ECK) PCT. The workshops were held at the Centre for Health Services Studies, University of Kent. The aims were to increase participants’ understanding of social marketing and evaluation, and to develop an action plan that will meet any training needs and identify how research support can be provided until the end of the project. Following a scoping exercise conducted by the CHSS Social Marketing Team between April and June 2009, a need was identified to inform social marketing project leads of the concept and application of social marketing, and help with the evaluation of social marketing initiatives

Evaluating Social Marketing - Final Report

The main purpose of this one-year project was to locate, monitor and evaluate health promotion interventions that are characterised and embedded in the use of social marketing techniques. This project was comprised of two phases – The aims of Phase One were to: Identify and prioritise those projects that have used social marketing techniques Agree an evaluation plan in collaboration with local and national experts The completion of the scoping exercise for Phase One highlighted that the research team needed to move away from any explicit evaluation activities and towards assisting projects with conducting their own evaluation. The revised aims of Phase Two were to: Facilitate with the integration of robust evaluation methods into project planning and to advise on the collection and analysis of evaluation data Support the organisation in enhancing their strategic understanding of social marketing and its potential in health promotion activity Anticipate that projects using social marketing techniques successfully could be at the forefront of dissemination, providing illustrations of the practical application of technique