Pulpal Tissue in Bilateral Talon Cusps of Primary Central Incisors: Report of a Case

Talon cusp is a tooth anomaly that affects both the primary and the permanent dentitions. However, the occurrence of this anomalous cusp is rather infrequent in the primary dentition. Only 7 cases of bilateral talon cusps affecting the primary teeth have been reported in the dental literature. This is a case report of bilateral talon cusps on primary maxillary central incisors whose histologic evaluation revealed the existence of pulpal tissue in the anomalous cusps

Effects of topotecan treatment on nasal, buccal, and lingual mucosa in the rabbit: light and transmission electron microscopic evaluation

WOS: 000243000300015PubMed: 17063354Anticancer agents may cause side effects and some of which may be dose dependant. It is important for clinicians to see the effects on tissues histopathologically. The aim of this study was to investigate the effects of topotecan (hycamtin((R))), a topoisomerase I inhibiting anticancer agent, on nasal, buccal, and lingual mucosa of rabbits. The study was carried out in two groups each consisting of 20 rabbits. Rabbits in group I received i.v. topotecan (0.5 mg/kg once daily) for 3 days. Rabbits in group II received i.v. topotecan (0.25 mg/kg once daily) for 3 days. In group I and II, biopsies from the nasal, buccal, and lingual mucosa were taken on the fourth (1 day after the 3-day topotecan treatment) and 15th day (12 days after the 3-day topotecan treatment). Light and transmission electron microscopic (TEM) observations have shown that nasal mucosa was not affected by topotecan administration. Topotecan treatment resulted in the formation of some ulcerative lesions in the lingual mucosa especially on the lower surface of the tongue. On the dorsal surface, the epithelium showed highly edematous and degenerating cells and separations in the stratum granulosum. In the buccal mucosa, effects were similar. In lingual and buccal mucosa, healing was observed on the 15th day. The oral (lingual and buccal) mucosal side effects of topotecan were observed as reversible and not dose dependent. It was concluded that these side effects are not severe, and topotecan may be used safely in cancer treatment

The effect of subcutaneously injected nicotine on achilles tendon healing in rabbits

The objective of this study was to evaluate the effect of subcutaneously injected nicotine on transversely transected and sutured achilles tendon healing in an experimental rabbit model. Adult New Zealand rabbits (n=22) weighting 3,000-3,500 g were used in this experimental study. Rabbits were randomly divided into two groups. Achilles tendon was transversely incised and repaired in all animals. In the experiment group subcutaneous injection of Nicotine tartrate 3 mg/kg/day was done. In the control group Serum physiologic injection was done at the same dosage. The injections were made three times a day in equal dosages. Nicotine and SF injections were made until the end of the 8-week, and then all animals were euthanized. Both light microscopic and electron microscopic evaluations were made on 14 animals. In N group light microscopic evaluation showed a visible gap in repair site. The total tendon score represented in N group was less than in SF group. The statistical analysis of the groups was significantly different for total tendon scores (P=0.002). Beside this electron microscopic examination showed inactive and degenerated fibroblasts and irregular collagen fibrils around them as well as collagen synthesis interruption in N group. Biomechanical evaluation was made on eight animals. The average tensile strength values in Group N (139.47 +/- 44.55 N) were significantly lower than those in Group SF (265.9 +/- 39.01 N) (z=2.309, P=0.029). Nicotine is the major chemical component common to all cigarettes and previously has been shown to affect wound and fracture healing adversely. The results of this study show that nicotine impairs achilles tendon healing after a surgical repair

Effects Of Intra-Articular Administration Of Autologous Bone Marrow Aspirate On Healing Of Full-Thickness Meniscal Tear: An Experimental Study On Sheep

Objective: The aim of this study was to evaluate the effects of bone marrow-derived mesenchyinal stem cell and bone marrow elements On the healing Of meniscal tears. Methods: This study was performed on twelve, 2-year-old male Tahirova sheep. La each subject, one knee was used for experiment purposes and the other knee was used as a control. After creating a longitudinal full-thickness tear in the red-white zone of the medial meniscus, aspirated autologous bone marrow material was injected into the tear site in the experiment group. The control group received no intervention for secondary healing. Results: In the macroscopic evaluation of meniscus, a bridging reparation tissue and adhesion Were observed between the rims of the tear in the experiment group. There was no statistical difference in collagen fibril formation between the groups (p=0.16). There was significantly more neovascularization in the experiment group than the control group (p=0.003). The cell count was also a significantly higher in the experiment group (p=0.004) and formation Of cartilage plaques was More frequent in the experiment group (p=0.016). There was no evidence suggesting intrinsic repair in the meniscus of control group by light and electron microscopy. Conclusion: An injection Of bone marrow into the meniscus tear site improves healing in a meniscal tear model as demonstrated by both light and electron microscopic findings.WoSScopu

Immunopathology in drug resistant mesial temporal lobe epilepsy with different types of hippocampal sclerosis

<p><b>Purpose:</b> There is evidence that autoimmunity has a specific role in temporal lobe seizures of limbic encephalitis patients. Our aim in this study was to investigate any histopathological clues of autoimmune process in refractory temporal lobe epilepsy (TLE) patients with different pathologically proven hippocampal sclerosis (HS) types.</p> <p><b>Methods:</b> 22 patients who had undergone epilepsy surgery due to mesial TLE-HS were included. The sera of patients are tested for neuronal antibodies to N-methyl-D-aspartate receptors (NMDAR), leucine-rich, glioma inactivated 1 (LGI1), contactin-associated protein 2 (CASPR2), α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR), gamma-aminobutyric acid B receptor (GABA_BR) and glutamic acid decarboxylase (GAD). Pathological and immunohistochemical investigations including neuronal nuclei (NeuN), NMDAR, GAD, glial fibrillary acidic protein (GFAP), CD8⁺-CD3⁺ lymphocytes and immunoglobulin G (IgG) were done. Patients were grouped according to type of HS. Clinical features and immunohistochemical changes were defined in these groups.</p> <p><b>Results:</b> Available sera of 15 patients did not have any neuronal antibodies. Thirteen of 22 patients had HS type 1, three had HS type 2 and two had HS type 3. According to immunohistochemical investigations CD3⁺ and CD8⁺ T cell infiltration was more prominent in the hippocampus of patients with classical HS (International League Against Epilepsy (ILAE) Type 1 HS) and there was a significant negative correlation between epilepsy duration and numbers of CD3⁺-CD8⁺ lymphocytes in temporal lobe parenchyma.</p> <p><b>Conclusion:</b> The role of T cell-mediated immunopathology and immunopathological difference in a variety of drug resistant TLE-H2S patients was suggested. These findings can be helpful in understanding the epileptogenicity of HS.</p

Rosuvastatin Induces Apoptosis in Cultured Human Papillary Thyroid Cancer Cells

Statins show antiproliferative activity in various cancer cells. The aim of this study was to evaluate the effects of rosuvastatin treatment on papillary thyroid carcinoma. The papillary thyroid carcinoma (B-CPAP) and normal (Nthy-ori 3-1) thyroid cell lines were treated with rosuvastatin at 12.5, 18.5, 25, 50, 100, and 200 mu M concentrations. After 48 and 72 h of rosuvastatin treatment, 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, Ki-67 immunolabeling, FACS analysis, electron microscopy, caspase-3, and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) analysis were performed. Decreased cell viability and G1 phase arrest were detected in papillary thyroid cell line treated with rosuvastatin. Positive immunoreactivity of Ki-67 and dose-dependent increase in S phase on Nthy-ori 3-1 cells were also detected. B-CPAP cells showed intense vacuolisation and autophagosomes with low concentrations and 48 h incubations, while Nthy-ori 3-1 cells showed these changes at higher concentrations. A decrease in the percentage of cells showing autophagy was determined with increasing concentrations of rosuvastatin in B-CPAP cells. Rosuvastatin treatment also caused a dose-and time-dependent increase in caspase-3 activity and apoptotic index by TUNEL assay in B-CPAP cells compared with the Nthy-ori 3-1 cells. Apoptotic cells with nuclear condensation and fragmentation were observed in B-CPAP cell line. Rosuvastatin induced autophagic changes in B-CPAP papillary thyroid cancer cells in lower doses and caused a shift from autophagy to apoptosis. Rosuvastatin may be an alternative treatment for refractory papillary thyroid cancer. Further in vivo studies are necessary to clarify the effects of rosuvastatin in papillary thyroid carcinoma and the clinical implications of rosuvastatin treatment. Journal of Endocrinology (2011) 210, 105-115WoSScopu

Histoloji Laboratuvar Yardımcı Kitabı

Histoloji; Tıp eğitiminde insana ait birçok farklı hücre, doku ve organların yapılanmalarını araştıran, yorumlayan, fonksiyonel aktiviteleri ile ilişkilendiren ve mikroskobik görüntülenmelerini içeren önemli bir sağlık bilimidir. Bu kitap çeşitli hücre, doku ve organların histolojilerini ve bunlara ait mikrografları içermektedir. Kitabın amacı öğrencilerin laboratuvarda preparatları mikroskop altında incelerken geçirdikleri zamanı verimli kılmak ve bu sırada hücrenin biyolojik aktivitesine yönelik fonksiyonel yapılanmasını histokimyasal boyanma özellikleriyle ilişkilendirmelerini sağlamaktır. Kitaptaki her bölüm; mikrograflar ve kısa açıklamaları ile organ, doku ve hücrelerin temel özellikleri hakkında bilgi vermektedir. Preparatlardan alınan mikrograflar; Hacettepe Üniversitesi Tıp Fakültesi Histoloji ve Embriyoloji Anabilim Dalı; laboratuvar teknisyenleri, araştırma görevlileri, uzmanları ve akademik personelinin yıllar içinde katkıda bulunduğu emeğidir