Distinct roles for Arabidopsis SUMO protease ESD4 and its closest homolog ELS1

SUMO conjugation affects a broad range of processes in Arabidopsis thaliana, including flower initiation, pathogen defense, and responses to cold, drought and salt stress. We investigated two sequence-related SUMO-specific proteases that are both widely expressed and show that they differ significantly in their properties. The closest homolog of SUMO protease ESD4, ESD4-LIKE SUMO PROTEASE 1 (ELS1, alternatively called AtULP1a) has SUMO-specific proteolytic activity, but is functionally distinct from ESD4, as shown by intracellular localization, mutant phenotype and heterologous expression in yeast mutants. Furthermore, we show that the growth defects caused by loss of ESD4 function are not due to increased synthesis of the stress signal salicylic acid, as was previously shown for a SUMO ligase, indicating that impairment of the SUMO system affects plant growth in different ways. Our results demonstrate that two A. thaliana SUMO proteases showing close sequence similarity have distinct in vivo functions

Arbuscular Mycorrhizal Fungi and Plant Chemical Defence : Effects of Colonisation on Aboveground and Belowground Metabolomes

Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizophagus irregularis on the chemical defences, spanning above- and below-ground tissues, in its host-plant ragwort (Senecio jacobaea). We used a non-targeted metabolomics approach to profile, and where possible identify, compounds induced by AMF colonisation in both roots and shoots. Metabolomics analyses revealed that 33 compounds were significantly increased in the root tissue of AMF colonised plants, including seven blumenols, plant-derived compounds known to be associated with AMF colonisation. One of these was a novel structure conjugated with a malonyl-sugar and uronic acid moiety, hitherto an unreported combination. Such structural modifications of blumenols could be significant for their previously reported functional roles associated with the establishment and maintenance of AM colonisation. Pyrrolizidine alkaloids (PAs), key anti-herbivore defence compounds in ragwort, dominated the metabolomic profiles of root and shoot extracts. Analyses of the metabolomic profiles revealed an increase in four PAs in roots (but not shoots) of AMF colonised plants, with the potential to protect colonised plants from below-ground organisms

Universal Alcohol/Drug Screening in Prenatal Care: A Strategy for Reducing Racial Disparities? Questioning the Assumptions

Agencies and organizations promoting universal screening for alcohol and drug use in prenatal care argue that universal screening will reduce White versus Black racial disparities in reporting to Child Protective Services (CPS) at delivery. Yet, no published research has assessed the impact of universal screening on reporting disparities or explored plausible mechanisms. This review defines two potential mechanisms: Equitable Surveillance and Effective Treatment and identifies assumptions underlying each mechanism. It reviews published literature relating to each assumption. Research relating to assumptions underlying each mechanism is primarily inconclusive or contradictory. Thus, available research does not support the claim that universal screening for alcohol and drug use in prenatal care reduces racial disparities in CPS reporting at delivery. Reducing these reporting disparities requires more than universal screening

Root Herbivore Effects on Aboveground Multitrophic Interactions: Patterns, Processes and Mechanisms

In terrestrial food webs, the study of multitrophic interactions traditionally has focused on organisms that share a common domain, mainly above ground. In the last two decades, it has become clear that to further understand multitrophic interactions, the barrier between the belowground and aboveground domains has to be crossed. Belowground organisms that are intimately associated with the roots of terrestrial plants can influence the levels of primary and secondary chemistry and biomass of aboveground plant parts. These changes, in turn, influence the growth, development, and survival of aboveground insect herbivores. The discovery that soil organisms, which are usually out of sight and out of mind, can affect plant-herbivore interactions aboveground raised the question if and how higher trophic level organisms, such as carnivores, could be influenced. At present, the study of above-belowground interactions is evolving from interactions between organisms directly associated with the plant roots and shoots (e.g., root feeders - plant - foliar herbivores) to interactions involving members of higher trophic levels (e.g., parasitoids), as well as non-herbivorous organisms (e.g., decomposers, symbiotic plant mutualists, and pollinators). This multitrophic approach linking above- and belowground food webs aims at addressing interactions between plants, herbivores, and carnivores in a more realistic community setting. The ultimate goal is to understand the ecology and evolution of species in communities and, ultimately how community interactions contribute to the functioning of terrestrial ecosystems. Here, we summarize studies on the effects of root feeders on aboveground insect herbivores and parasitoids and discuss if there are common trends. We discuss the mechanisms that have been reported to mediate these effects, from changes in concentrations of plant nutritional quality and secondary chemistry to defense signaling. Finally, we discuss how the traditional framework of fixed paired combinations of root- and shoot-related organisms feeding on a common plant can be transformed into a more dynamic and realistic framework that incorporates community variation in species, densities, space and time, in order to gain further insight in this exciting and rapidly developing field

Mapping coastal land use changes 1965–2014: methods for handling historical thematic data

This paper describes a national analysis of coastal land use change in England, Wales and Northern Ireland. It compares a survey conducted by volunteers in 1965 with the 2014 update created using digital topographic data and aerial photography in an open source GIS. The paper reviews the origins and impacts of differences in the way that land use classes are measured and reported, and highlights a generic issue when comparing thematic data. This is, that thematic data are frequently subject to changes in the way that classes are conceived, measured and classified with the result that similarly named classes in different datasets may have very different spatial extents with very different meanings and underlying semantics, even when there is little difference in reality. The sources and nature of such variation in landscape conceptualisations are discussed and placed into the context of historical GIS (HGIS) analyses of thematic change. The critical issue is the need to separate actual differences on the ground from artefactual differences arising from methodological inconsistencies to support robust statistical analyses. A set of rubrics for updating historical thematic data is suggested to minimise the potential for such inconsistencies. These are applied to the National Trust's 1965 Neptune coastal land use survey and its 2014 update to quantify land use changes. The results describe the magnitude and direction of change, provide insights into the developmental pressures experienced at the coast and demonstrate the positive impacts of the Trust's management. Of potentially wider research interest to the HGIS and related research communities is the consideration of methods for mapping and quantifying thematic and areal changes. This is an underdeveloped research area in HGIS, when compared with the extensive methods for dealing with counts and boundary changes (e.g. census areas), but one that is critical for robust analysis of historical cartographic data

Analysis of human exposure to landslides with a GIS multiscale approach

AbstractDecision-making plays a key role in reducing landslide risk and preventing natural disasters. Land management, recovery of degraded lands, urban planning, and environmental protection in general are fundamental for mitigating landslide hazard and risk. Here, we present a GIS-based multi-scale approach to highlight where and when a country is affected by a high probability of landslide occurrence. In the first step, a landslide human exposure equation is developed considering the landslide susceptibility triggered by rain as hazard, and the population density as exposed factor. The output, from this overview analysis, is a global GIS layer expressing the number of potentially affected people by month, where the monthly rain is used to weight the landslide hazard. As following step, Logistic Regression (LR) analysis was implemented at a national and local level. The Receiver Operating Characteristic indicator is used to understand the goodness of a LR model. The LR models are defined by a dependent variable, presence–absence of landslide points, versus a set of independent environmental variables. The results demonstrate the relevance of a multi-scale approach, at national level the biophysical variables are able to detect landslide hotspot areas, while at sub-regional level geomorphological aspects, like land cover, topographic wetness, and local climatic condition have greater explanatory power