Direct measurement of molecular stiffness and damping in confined water layers

We present {\em direct} and {\em linear} measurements of the normal stiffness and damping of a confined, few molecule thick water layer. The measurements were obtained by use of a small amplitude (0.36 $\textrm{\AA}$), off-resonance Atomic Force Microscopy (AFM) technique. We measured stiffness and damping oscillations revealing up to 7 layers separated by 2.56 $\pm$ 0.20 $\textrm{\AA}$. Relaxation times could also be calculated and were found to indicate a significant slow-down of the dynamics of the system as the confining separation was reduced. We found that the dynamics of the system is determined not only by the interfacial pressure, but more significantly by solvation effects which depend on the exact separation of tip and surface. Thus ` solidification\rq seems to not be merely a result of pressure and confinement, but depends strongly on how commensurate the confining cavity is with the molecule size. We were able to model the results by starting from the simple assumption that the relaxation time depends linearly on the film stiffness.Comment: 7 pages, 6 figures, will be submitted to PR

Comparisons of Salmonella conjugation and virulence gene hyperexpression mediated by rumen protozoa from domestic and exotic ruminants

Recent studies have identified a phenomenon in which ciliated protozoa engulf Salmonella and the intra-protozoal environment hyperactivates virulence gene expression and provides a venue for conjugal transfer of antibiotic resistance plasmids. The former observation is relegated to Salmonella bearing the SGI1 multiresistance integron while the latter phenomenon appears to be a more generalized event for recipient Salmonella. Our previous studies have assessed virulence gene hyperexpression only with protozoa from the bovine rumen while conjugal transfer has been demonstrated in rumen protozoa from cattle and goats. The present study examined virulence gene hyperexpression for Salmonella exposed to rumen protozoa obtained from cattle, sheep, goats, or two African ruminants (giraffe and bongo). Conjugal transfer was also assessed in these protozoa using Salmonella as the recipient. Virulence gene hyperexpression was only observed following exposure to the rumen protozoa from cattle and sheep while elevated virulence was also observed in these animals. Conjugal transfer events were, however, observed in all protozoa evaluated. It therefore appears that the protozoa-based hypervirulence is not universal to all ruminants while conjugal transfer is more ubiquitous

Monitoring of International Space Station Telemetry Using Shewhart Control Charts

Shewhart control charts have been established as an expedient method for analyzing dynamic, trending data in order to identify anomalous subsystem performance as soon as such performance would exceed a statistically established baseline. Additionally, this leading indicator tool integrates a selection methodology that reduces false positive indications, optimizes true leading indicator events, minimizes computer processor unit duty cycles, and addresses human factor concerns (i.e., the potential for flight-controller data overload). This innovation leverages statistical process control, and provides a relatively simple way to allow flight controllers to focus their attention on subtle system changes that could lead to dramatic off-nominal system performance. Finally, this capability improves response time to potential hardware damage and/or crew injury, thereby improving space flight safety. Shewhart control charts require normalized data. However, the telemetry from the ISS Early External Thermal Control System (EETCS) was not normally distributed. A method for normalizing the data was implemented, as was a means of selecting data windows, the number of standard deviations (Sigma Level), the number of consecutive points out of limits (Sequence), and direction (increasing or decreasing trend data). By varying these options, and treating them like dial settings, the number of nuisance alerts and leading indicators were optimized. The goal was to capture all leading indicators while minimizing the number of nuisances. Lean Six Sigma (L6S) design of experiment methodologies were employed. To optimize the results, Perl programming language was used to automate the massive amounts of telemetry data, control chart plots, and the data analysis

Archaeological Investigations at Four San Antonio Missions: Mission Trails Underground Conversion Project

This report contains the results of archaeological work performed by the Center for Archaeological Research (CAR) at The University of Texas San Antonio (UTSA) for City Public Service (CPS). The archaeological investigation and monitoring for the Mission Trails Underground Conversion Project was carried out under Texas Historical Commission (THC) Permit Number: 2020, and the work was conducted at each of the four historical San Antonio missions which make up San Antonio Missions National Historical Park. The Mission Trails Underground Conversion Project was created to enhance and upgrade electrical and utility connections by replacing aboveground connections with underground connections at each ofthe four missions. Mission San Juan (41BX5)-In October of 1998, personnel from CAR began archaeological investigations at Mission San Juan Capistrano in advance of the proposed installation of underground utility lines for the Mission Trails Underground Conversion Project. The purpose of the investigation was to test for intact, buried cultural features in advance of trenching activities involved in the relocation of utility lines and connections. CAR archaeologists developed a plan regarding the area to be impacted which included ten 1 x 1-m excavations units and a series of twelve shovel tests along the proposed path of the utility trench. Excavation units uncovered evidence of existing buildings, wall foundations and Colonial-period flooring episodes, revealing new data west of the known structures and not previously accounted for. Further excavations are warranted to determine the nature of features such as a Colonial-period wall revealed near one of the excavation units. This material discovered by the archaeological investigations resulted in the altering of the projected utility trench alignment to avoid disturbing deposits. Further archaeological investigations are recommended for the newly revealed features. Mission San Jose (41BX3)- In January of 1999, personnel from CAR began archaeological investigations at Mission San Jose y San Miguel de Aguayo in advance of the proposed installation of underground utility lines for the Mission Trails Underground Conversion Project. The purpose of the investigation was to test for intact, buried cultural features in advance of trenching activities involved in the relocation of utility lines and connections. CAR archaeologists conducted a series of shovel tests along the center-lines of the proposed utility trenches to identifY areas of possible impact to intact Spanish Colonial cultural materials. In addition to shovel testing, monitoring of the trenching was conducted in areas where the possibility of impacting cultural material was high. An early-twentieth century trash midden was encountered during monitoring of the trench where it paralleled the Service Drive in the northwest comer of the Mission compound. This material did not warrant altering the course of the trench, and no further archaeological work is recommended at this time. Mission Concepcion (41BX12)- In January of 1999, personnel from CAR began archaeological investigations at Mission Nuestra Senora de la Purisima Concepcion as part of the Mission Trails Underground Conversion Project. A construction crew, while excavating a manhole designed to provide access to underground electrical utilities, had unearthed a large number of animal bones. After concluding a large feature had been unexpectedly impacted, CAR conducted test excavations to ascertain the nature of this feature. It was determined to be a section of an acequia which had been refilled during the Colonial period with construction debris and trash, including a large number of animal bones. Test units and the original manhole excavations allowed for a profile of the acequia to be constructed. This information combined with previous archaeological work and historical research, allowed a tentative proposal on the sequence of acequia construction and re-routing for the Concepcion mission. Monitoring of other underground work conducted in the area resulted in the discovery of a location of a Colonial-period wall, southeast of the Visitors\u27 Center, which may be a portion of the original south wall of the mission. It is recommended that if any further work is required, consideration should be given to testing and/or monitoring to reduce the risk of negative impact to historic remains. Mission Espada (41BX4)- In July of 1999, personnel from CAR began archaeological investigations at Mission San Francisco de la Espada in advance of the proposed installation of underground utility lines for the Mission Trails Underground Conversion Project. From initial discussions, it was noted that the proximity of the proposed route of the underground utility trench could have an impact on the old compound walls of the mission and the path was altered to avoid any possible impact. A series of thirteen shovel tests were conducted in advance along the altered path of the proposed utility trench as it progressed from the southwest comer of the mission compound across the dry irrigation ditch or acequia to the northwest. Monitoring of the trenching activities followed with no significant deposits being exposed. No further work is recommended within the project area, but if future construction is to occur in the area between the utility trench and the Church at Mission Espada, monitoring should then be considered

The Unusual Evolution of Hurricane Arthur 2014

Hurricane Arthur (2014) was an early season hurricane that had its roots in a convective complex in the Southern Plains of the U.S. As the complex moved into northern Texas, a Mesoscale Convective Vortex (MCV) formed and drifted towards the east of the southern U.S. for a few days before emerging over the southwest Atlantic near South Carolina. The MCV drifted south and slowly acquired tropical characteristics, eventually becoming a Category 2 hurricane that would affect much of eastern North Carolina prior to the 4th of July holiday weekend. Arthur continued up the coast, brushing portions of southeast New England and merged with an upper-level low, completing a full tropical to extratropical-transition in the process, producing damaging wind gusts in portions of the Canadian Maritimes. As part of the GOES-R and JPSS Satellite Proving Grounds, multiple proxy and operational products were available to analyze and forecast this complex evolution. The Storm Prediction Center had products available to monitor the initial severe thunderstorm aspect, while the National Hurricane Center and Ocean Prediction Center were able to monitor the tropical and extratropical transition of Arthur using various convective and red, green, blue (RGB) products that have been introduced in recent years. This paper will discuss Arthur's evolution through the eyes of the various Satellite Proving Ground demonstrations

Axonal plasticity underpins the functional recovery following surgical decompression in a rat model of cervical spondylotic myelopathy.

Cervical spondylotic myelopathy (CSM) is the most common spinal cord disorder and a major cause of disability in adults. Improvements following surgical decompression are limited and patients often remain severely disabled. Post mortem studies indicate that CSM is associated with profound axonal loss. However, our understanding of the pathophysiology of CSM remains limited.To investigate the hypothesis that axonal plasticity plays a role in the recovery following surgical decompression, we adopted a novel preclinical model of mild to moderate CSM. Spinal cord compression resulted in significant locomotor deterioration, increased expression of the axonal injury marker APP, and loss of serotonergic fibres. Surgical decompression partially reversed the deficits and attenuated APP expression. Decompression was also associated with axonal sprouting, reflected in the restoration of serotonergic fibres and an increase of GAP43 expression. The re-expression of synaptophysin indicated the restoration of functional synapses following decompression. Promoting axonal plasticity may therefore be a therapeutic strategy for promoting neurological recovery in CSM.Qatar Foundation, National Institute for Health Research (Clinician Scientist Award Grant ID: CS-2015-15-023), Royal Australasian College of Surgeons (Reg Worcester Research Fellowship), Neurosurgical Society of Australasia (Research Scholarship), Wellcome Trust, Medical Research CouncilThis is the final version of the article. It first appeared from BioMed Central via http://dx.doi.org/10.1186/s40478-016-0359-

Expression Profile Matrix of Arabidopsis Transcription Factor Genes Suggests Their Putative Functions in Response to Environmental Stresses

Numerous studies have shown that transcription factors are important in regulating plant responses to environmental stress. However, specific functions for most of the genes encoding transcription factors are unclear. In this study, we used mRNA profiles generated from microarray experiments to deduce the functions of genes encoding known and putative Arabidopsis transcription factors. The mRNA levels of 402 distinct transcription factor genes were examined at different developmental stages and under various stress conditions. Transcription factors potentially controlling downstream gene expression in stress signal transduction pathways were identified by observed activation and repression of the genes after certain stress treatments. The mRNA levels of a number of previously characterized transcription factor genes were changed significantly in connection with other regulatory pathways, suggesting their multifunctional nature. The expression of 74 transcription factor genes responsive to bacterial pathogen infection was reduced or abolished in mutants that have defects in salicylic acid, jasmonic acid, or ethylene signaling. This observation indicates that the regulation of these genes is mediated at least partly by these plant hormones and suggests that the transcription factor genes are involved in the regulation of additional downstream responses mediated by these hormones. Among the 43 transcription factor genes that are induced during senescence, 28 of them also are induced by stress treatment, suggesting extensive overlap responses to these stresses. Statistical analysis of the promoter regions of the genes responsive to cold stress indicated unambiguous enrichment of known conserved transcription factor binding sites for the responses. A highly conserved novel promoter motif was identified in genes responding to a broad set of pathogen infection treatments. This observation strongly suggests that the corresponding transcription factors play general and crucial roles in the coordinated regulation of these specific regulons. Although further validation is needed, these correlative results provide a vast amount of information that can guide hypothesis-driven research to elucidate the molecular mechanisms involved in transcriptional regulation and signaling networks in plants

Sixth Annual NASA Ames Space Science and Astrobiology Jamboree

Welcome to the Sixth Annual NASA Ames Research Center, Space Science and Astrobiology Jamboree at NASA Ames Research Center (ARC). The Space Science and Astrobiology Division consists of over 60 Civil Servants, with more than 120 Cooperative Agreement Research Scientists, Post-Doctoral Fellows, Science Support Contractors, Visiting Scientists, and many other Research Associates. Within the Division there is engagement in scientific investigations over a breadth of disciplines including Astrobiology, Astrophysics, Exobiology, Exoplanets, Planetary Systems Science, and many more. The Division's personnel support NASA spacecraft missions (current and planned), including SOFIA, K2, MSL, New Horizons, JWST, WFIRST, and others. Our top-notch science research staff is spread amongst three branches in five buildings at ARC. Naturally, it can thus be difficult to remain abreast of what fellow scientific researchers pursue actively, and then what may present and/or offer regarding inter-Branch, intra-Division future collaborative efforts. In organizing this annual jamboree, the goals are to offer a wholesome, one-venue opportunity to sense the active scientific research and spacecraft mission involvement within the Division; and to facilitate communication and collaboration amongst our research scientists. Annually, the Division honors one senior research scientist with a Pollack Lecture, and one early career research scientist with an Outstanding Early Career Space Scientist Lecture. For the Pollack Lecture, the honor is bestowed upon a senior researcher who has made significant contributions within any area of research aligned with space science and/or astrobiology. This year we are pleased to honor Linda Jahnke. With the Early Career Lecture, the honor is bestowed upon an early-career researcher who has substantially demonstrated great promise for significant contributions within space science, astrobiology, and/or, in support of spacecraft missions addressing such disciplines. This year we are pleased to honor Amanda Cook. We hope that you will make time to join us for the day in meeting fellow Division members, expanding knowledge of our activities, and creating new collaborations within the Space Science and Astrobiology Division