Variation in performance of beech saplings of 7 European provenances under shade and full light conditions

The use of beech seedlings from South-East European and North-West (NW) provenances for underplanting in coniferous forests in North-West Europe was investigated by means of experimental shading. The effects of this treatment on survival, morphology, phenology, physiology and growth were analysed by applying an individual plant growth model integrating these aspects. It was concluded that plant performance under full-light conditions are representative of shaded conditions, so good performing provenances can be selected in a field situation. It was further concluded that good performing South-East European seedlings can be used in North-West European conditions. The modelling results indicated an interesting trade-off between height growth and biomass increase and different provenances show different strategies. This allows selection of suitable provenances for specific situations, e.g. when beech seedlings need to compete with other plant species in the understorey

The Ecological Adaptive Significance of Littoraria Scabra for Their Survivorship in Extreme Mangrove Environment of Tombariri

Penelitian ini menemukan bahwa signifikansi ekologi Littoraria scabra untuk survival di mikrohabitat mangrove Tombariri yang ekstrim adalah sebagai berikut berfungsi sebagai tempat istirahat, menghindari perendaman air, mencari makanan, meletakkan larva, menghindar cahaya dan bersembunyi dari predator-predator. Signifikansi L. scabra distribusi spasial mengelompok untuk mereduksi stres kekeringan, suhu, terlepas di substrat akibat gelombang, aktivitas makan, reproduksi, kecocokan habitat. Signifikansi distibusi mengelompok dengan populasi tinggi untuk mengimbangi kuatnya predasi serta merespons tekanan seleksi alam yang kuat demi kelangsungan hidupnya

Personalised electronic messages to improve sun protection in young adults

The incidence of all skin cancers, including melanoma, continues to rise. It is well known that ultraviolet (UV) radiation is the main environmental risk factor for skin cancer, and excessive exposure at a young age increases the risk of developing skin cancer. The aim of this study was to determine the acceptability and feasibility of delivering sun protection messages via electronic media such as short message services (SMS) to people 18-40 years, and explore factors associated with their acceptability. Overall, 80% of participants agreed that they would like to receive some form of sun protection advice; of these, 20% prefer to receive it via SMS and 42% via email. Willingness to receive electronic messages about the UV index was associated with being unsure about whether a suntanned person would look healthy and greater use of sun protection in the past. Careful attention to message framing and timing of message delivery and focus on short-term effects of sun exposure such as sunburn and skin ageing should increase the acceptability of such messages to young people. We conclude that sun protection messages delivered to young adults via electronic media appear feasible and acceptable

Online data services at the Belgian Marine Datacentre

Over the past few years, the Management Unit of the North Sea Mathematical Models (MUMM) has built up a centre of human and technical expertise oriented towards the management and the analysis of marine environmental data. The Belgian Marine Data Centre (BMDC) serves as national repository and processing centre for marine and environmental data and ensures a continuous and scientifically sound data flow between data producers and end-users of marine and environmental data collected in the frame of national and international research and monitoring programmes. The data cover most domains of oceanology as there are: physico-chemical, optical parameters, biodiversity, hydrodynamics, sedimentology, geography and human interest. Most of the datasets relate to the Belgian Continental Shelf, the Scheldt estuary and its surrounding areas.To promote the use of a high variety of data sets, different tools are constantly being developed and are presented on our website (www.mumm.ac.be/datacentre). An interface to the integrated database on the quality of the marine environment was developed. This database mainly contains the results of measurements and observations in situ and laboratory analyses of air, water, sediment and biota samples. Besides an extensive inventory of the database and an advanced request form with several selection criteria, some more straightforward queries and geographical selection tools are being developed for a faster and easier access to a specific dataset. Data can freely be downloaded. Once the data are obtained, a spatial analysis tool is freely and online available for the visualization on an interactive map. A lot of documentation accompanying the data is online available. The strongest feature of this system is the common underlying structure for different kinds and sources of data. This opens the possibility to compare biodiversity data, physico-chemical data, sedimentological data and historical data.Another database at MUMM, the real-time data acquisition system ‘ODAS’ stores the physical and chemical parameters measured onboard of the research vessel RV Belgica since 1984. The along -track data are published online shortly after the end of the measurement campaign together with all other information like the campaign reports, cruise tracks, … This is a valuable source of basic information for the scientists

Online data services at the Belgian Marine Datacentre

Over the past few years, the Management Unit of the North Sea Mathematical Models (MUMM) has built up a centre of human and technical expertise oriented towards the management and the analysis of marine environmental data. The Belgian Marine Data Centre (BMDC) serves as national repository and processing centre for marine and environmental data and ensures a continuous and scientifically sound data flow between data producers and end-users of marine and environmental data collected in the frame of national and international research and monitoring programmes. The data cover most domains of oceanology as there are: physico-chemical, optical parameters, biodiversity, hydrodynamics, sedimentology, geography and human interest. Most of the datasets relate to the Belgian Continental Shelf, the Scheldt estuary and its surrounding areas.To promote the use of a high variety of data sets, different tools are constantly being developed and are presented on our website (www.mumm.ac.be/datacentre). An interface to the integrated database on the quality of the marine environment was developed. This database mainly contains the results of measurements and observations in situ and laboratory analyses of air, water, sediment and biota samples. Besides an extensive inventory of the database and an advanced request form with several selection criteria, some more straightforward queries and geographical selection tools are being developed for a faster and easier access to a specific dataset. Data can freely be downloaded. Once the data are obtained, a spatial analysis tool is freely and online available for the visualization on an interactive map. A lot of documentation accompanying the data is online available. The strongest feature of this system is the common underlying structure for different kinds and sources of data. This opens the possibility to compare biodiversity data, physico-chemical data, sedimentological data and historical data.Another database at MUMM, the real-time data acquisition system ‘ODAS’ stores the physical and chemical parameters measured onboard of the research vessel RV Belgica since 1984. The along -track data are published online shortly after the end of the measurement campaign together with all other information like the campaign reports, cruise tracks, … This is a valuable source of basic information for the scientists

Revealing species communities in a spatial and temporal overlap

Storing physical and chemical values, optical spectra and sediment granulometry is already a cumbersome task; dealing with biological data even falls into a different category. Biologists tend to focus their attention to species of their interest while other specimens in the same sample are often underestimated. Meanwhile taxonomists are continuously revising the taxonomy resulting in a complete new set of relations between these taxa. Keeping track with both and meanwhile having a dataset up to date seems endless. At the Belgian Marine Data Centre we tried to think outside the box and came up with a solution to content both biologists and data managers. The last thing we aimed at is to create another web index to refer species, therefore we hooked up with the existing web based referencing systems. The need to get data about different food webs in a spatial and temporal overlap is answered by our hierarchical storage of taxa which allows selecting a predator at species level and at the same time selecting different prey species at lower taxonomic levels. As these species, and also the scientists, usually are not confined into ‘latitude longitude squares’ we elaborated the spatial selection tool which defines user specific polygons to base the selection of data upon. We will briefly present the structure of our relational database but specific attention will go out to the taxonomic and spatial parts. Incentives and discomforts to organize the data in this way, and our current web interface, will be demonstrated

Advances towards reliable identification and concentration determination of rare cells in peripheral blood

Through further development, integration and validation of micro-nano-bio and biophotonics systems FP7 CanDo is developing an instrument that will permit highly reproducible and reliable identification and concentration determination of rare cells in peripheral blood for two key societal challenges, early and low cost anti-cancer drug efficacy determination and cancer diagnosis/monitoring. A cellular link between the primary malignant tumour and the peripheral metastases, responsible for 90% of cancerrelated deaths, has been established in the form of circulating tumour cells (CTCs) in peripheral blood. Furthermore, the relatively short survival time of CTCs in peripheral blood means that their detection is indicative of tumour progression thereby providing in addition to a prognostic value an evaluation of therapeutic efficacy and early recognition of tumour progression in theranostics. In cancer patients however blood concentrations are very low (=1 CTC/1E9 cells) and current detection strategies are too insensitive, limiting use to prognosis of only those with advanced metastatic cancer. Similarly, problems occur in therapeutics with anti-cancer drug development leading to lengthy and costly trials often preventing access to market. The novel cell separation/Raman analysis technologies plus nucleic acid based molecular characterization of the CanDo platform will provide an accurate CTC count with high throughput and high yield meeting both key societal challenges. Being beyond the state of art it will lead to substantial share gains not just in the high end markets of drug discovery and cancer diagnostics but due to modular technologies also in others. Here we present preliminary DNA hybridization sensing results

Impaired nuclear import and viral incorporation of Vpr derived from a HIV long-term non-progressor

We previously reported an epidemiologically linked HIV-1 infected patient cohort in which a long-term non-progressor (LTNP) infected two recipients who then exhibited normal disease progression. Expression of patient-derived vpr sequences from each of the three cohort members in mammalian cells tagged with GFP revealed a significant reduction in Vpr nuclear import and virion incorporation uniquely from the LTNP, whereas Vpr from the two progressing recipients displayed normal localisation and virion incorporation, implying a link between efficient Vpr nuclear import and HIV disease progression. Importantly, an F72L point mutation in the LTNP was identified for the first time as being uniquely responsible for decreased Vpr nuclear import

The oxidation status of Mic19 regulates MICOS assembly.

The function of mitochondria depends on the proper organization of mitochondrial membranes. The morphology of the inner membrane is regulated by the recently identified mitochondrial contact site and cristae organizing system (MICOS) complex. MICOS mutants exhibit alterations in crista formation, leading to mitochondrial dysfunction. However, the mechanisms that underlie MICOS regulation remain poorly understood. MIC19, a peripheral protein of the inner membrane and component of the MICOS complex, was previously reported to be required for the proper function of MICOS in maintaining the architecture of the inner membrane. Here, we show that human and yeast MIC19 proteins undergo oxidation in mitochondria and require the mitochondrial intermembrane space assembly (MIA) pathway, which couples the oxidation and import of mitochondrial intermembrane space proteins for mitochondrial localization. Detailed analyses identified yeast Mic19 in two different redox forms. The form that contains an intramolecular disulfide bond is bound to Mic60 of the MICOS complex. Mic19 oxidation is not essential for its integration into the MICOS complex but plays a role in MICOS assembly and the maintenance of proper inner membrane morphology. These findings suggest that Mic19 is a redox-dependent regulator of MICOS function