Negotiation, bargaining, and discounts:generating WoM and local tourism development at the Tabriz bazaar, Iran

This paper examines the effects of negotiation intention, bargaining propensity, and discount satisfaction on word-of-mouth (WoM) behaviours for tourists visiting Tabriz bazaar, Iran. Data from 615-survey respondents highlight that tourists are motivated to conduct WoM behaviour when they are experientially satisfied with the opportunity to negotiate and bargain, and when they are satisfied with the discount they receive. This paper makes theoretical contributions to social exchange theory and presents managerial implications for policy-makers to generate tourism development

Vestigialization of an Allosteric Switch: Genetic and Structural Mechanisms for the Evolution of Constitutive Activity in a Steroid Hormone Receptor

An important goal in molecular evolution is to understand the genetic and physical mechanisms by which protein functions evolve and, in turn, to characterize how a protein's physical architecture influences its evolution. Here we dissect the mechanisms for an evolutionary shift in function in the mollusk ortholog of the steroid hormone receptors (SRs), a family of biologically essential transcription factors. In vertebrates, the activity of SRs allosterically depends on binding a hormonal ligand; in mollusks, however, the SR ortholog (called ER, because of high sequence similarity to vertebrate estrogen receptors) activates transcription in the absence of ligand and does not respond to steroid hormones. To understand how this shift in regulation evolved, we combined evolutionary, structural, and functional analyses. We first determined the X-ray crystal structure of the ER of the Pacific oyster Crassostrea gigas (CgER), and found that its ligand pocket is filled with bulky residues that prevent ligand occupancy. To understand the genetic basis for the evolution of mollusk ERs' unique functions, we resurrected an ancient SR progenitor and characterized the effect of historical amino acid replacements on its functions. We found that reintroducing just two ancient replacements from the lineage leading to mollusk ERs recapitulates the evolution of full constitutive activity and the loss of ligand activation. These substitutions stabilize interactions among key helices, causing the allosteric switch to become “stuck” in the active conformation and making activation independent of ligand binding. Subsequent changes filled the ligand pocket without further affecting activity; by degrading the allosteric switch, these substitutions vestigialized elements of the protein's architecture required for ligand regulation and made reversal to the ancestral function more complex. These findings show how the physical architecture of allostery enabled a few large-effect mutations to trigger a profound evolutionary change in the protein's function and shaped the genetics of evolutionary reversibility.</p

TEMPORAL AND SPATIAL OCCLUSION OF ADVANCED VISUAL INFORMATION CONSTRAIN EMERGENT COORDINATION OF ONE-HANDED CATCHING BEHAVIOURS

Introduction Dynamic interceptive actions, like catching, are performed under severe spatial and temporal constraints. Here, behavioral processes underpinning one-handed catching was examined using a novel spatial and temporal occlusion design which enabled precise control of pre-release visual information of an actor and a fully coupled action response. Methods An integrated video and ball projection machine was used to create four temporal occlusion and five spatial occlusion conditions of an actor throwing a ball towards participants. Twelve participants’ hand kinematics and gaze behaviors were recorded while attempting to catch a projected ball synchronized with the video footage. Results Temporal occlusion findings revealed that when footage was occluded at earlier time points, catching performance decreased. Movement onset of the catching hand and initiation of visual ball tracking emerged earlier when footage of the thrower was occluded at a later time point in the throwing action. Spatial occlusion did not affect catching success, although movement onset emerged later when increased visual information of the actor was occluded. Later movement onset was countered by greater maximum velocity of the catching hand. The final stages of action (e.g., grasping action of the hand) remained unchanged across both spatial and temporal conditions suggesting the later phases of the action were organised using ball flight information. Discussion Findings revealed how catching behaviors were continuously (re)organized and adapted as information became available in task performance, first by using kinematic information of a thrower's actions, and then by ball flight information. This highlighted the importance of maintaining information-movement coupling during performance of interceptive actions, with these behavioral adaptations having important implications for research that assesses interceptive skills based solely on pre-ball flight information, as in many current video-based simulation paradigms. Contact [email protected]

Interprofessional Learning Readiness: Health Policy Summit

Purpose: Interprofessional Education (IPE) and Health Policy are important components in health professional curricula. Students from business, communication sciences and disorders, dietetics, occupational therapy, nursing, and social work participated in an innovative IPE event working in an IPE group to apply discipline specific knowledge and propose solutions to the Medicaid Expansion gap in Virginia. Students presented their final proposals to legislators while advocating for issues important to their discipline. Methodology/Results: This study used the Readiness for Interprofessional Learning Scale (RIPLS) to examine student Teamwork and Collaboration, Professional Identity, and Roles and Responsibilities following participation in a Health Policy Summit. The results revealed a difference at baseline between health professions students and business students (N= 260) in their perception of teamwork and collaboration between groups. The themes of the question items found to be significant within the scale pre- and posttest were student perception of learning with other health-care/professional students, shared learning to help students understand their limitations, and welcoming opportunities to work with IPE students. Conclusion: This data indicates that there remains an opportunity to promote student perceptions of their abilities to participate in teamwork, collaborate significantly, and to understand the scope of their discipline specific knowledge and contributions to a team

The mitochondrial citrate/isocitrate carrier plays a regulatory role in glucose-stimulated insulin secretion.

Glucose-stimulated insulin secretion (GSIS) is mediated in part by glucose metabolism-driven increases in ATP/ADP ratio, but by-products of mitochondrial glucose metabolism also play an important role. Here we investigate the role of the mitochondrial citrate/isocitrate carrier (CIC) in regulation of GSIS. Inhibition of CIC activity in INS-1-derived 832/13 cells or primary rat islets by the substrate analogue 1,2,3-benzenetricarboxylate (BTC) resulted in potent inhibition of GSIS, involving both first and second phase secretion. A recombinant adenovirus containing a CIC-specific siRNA (Ad-siCIC) dose-dependently reduced CIC expression in 832/13 cells and caused parallel inhibitory effects on citrate accumulation in the cytosol. Ad-siCIC treatment did not affect glucose utilization, glucose oxidation, or ATP/ADP ratio but did inhibit glucose incorporation into fatty acids and glucose-induced increases in NADPH/NADP+ ratio relative to cells treated with a control siRNA virus (Ad-siControl). Ad-siCIC also inhibited GSIS in 832/13 cells, whereas overexpression of CIC enhanced GSIS and raised cytosolic citrate levels. In normal rat islets, Ad-siCIC treatment also suppressed CIC mRNA levels and inhibited GSIS. We conclude that export of citrate and/or isocitrate from the mitochondria to the cytosol is an important step in control of GSIS

The activity demands and physiological responses observed in professional ballet: A systematic review

The aim of this study was to systematically review research into the activity demands and physiological responses observed in professional ballet. PubMed, Web of Science, SPORTDiscus, and ProQuest were searched for original research relating to 1) the session- specific activity demands of professional ballet, 2) the general activity demands of professional ballet, 3) the immediate physiological responses to professional ballet, or 4) the delayed physiological responses to professional ballet. From an initial 7672 studies, 22 met the inclusion criteria. Methodological quality was assessed using the Mixed Methods Appraisal Tool and a modified Downs and Black Index. Professional ballet is intermittent; however, activity characteristics and intensity vary by session type and company rank. Performances involve high volumes of jumps (5.0 ± 4.9 jumps·min-1), pliés (11.7 ± 8.4 pliés·min-1), and lifts (men - 1.9 ± 3.3 lifts·min-1), which may result in near-maximal metabolic responses. Ballet classes are less metabolically intense than performance during both barre and centre ( 5 h·day-1), but half is spent at intensities below 3 METs. Evidence is mixed regarding the delayed physiological responses to professional ballet; however, metabolic and musculoskeletal adaptations are unlikely to occur from ballet alone. The mean Downs and Black score was 62%. Appraisal tools revealed that a lack of clarity regarding sampling procedures, no power calculation, and a poor quality of statistical analysis were common limitations of the included studies. Given the large working durations and high rates of jumps, pliés, and lifts, managing training loads and recovery may be a focus for strategies seeking to optimize dancer health and wellbeing. Ballet companies should provide dancers with opportunities and resources to engage in supplementary physical training. Further research is required into the physical demands of rehearsals and the longitudinal training loads undertaken by professional ballet dancers

An Uncertain Dominion: Irish Psychiatry, Methadone, and the Treatment of Opiate Abuse

This paper investigates some productive ambiguities around the medical administration of methadone in the Republic of Ireland. The tensions surrounding methadone maintenance therapy (MMT) are outlined, as well as the sociohistorical context in which a serious heroin addiction problem in Ireland developed. Irish psychiatry intervened in this situation, during a time of institutional change, debates concerning the nature of addiction, moral panics concerning heroin addiction in Irish society and the recent boom in the Irish economy, known popularly as the Celtic Tiger. A particular history of this sort illuminates how technologies like MMT become cosmopolitan, settling into, while changing, local contexts

Monitoring a simple hydrolysis process in an organic solid by observing methyl group rotation

We report a variety of experiments and calculations and their interpretations regarding methyl group (CH3) rotation in samples of pure 3-methylglutaric anhydride (1), pure 3-methylglutaric acid (2), and samples where the anhydride is slowly absorbing water from the air and converting to the acid [C6H8O3(1) + H2O → C6H10O4(2)]. The techniques are solid state 1H nuclear magnetic resonance (NMR) spin-lattice relaxation, single-crystal X-ray diffraction, electronic structure calculations in both isolated molecules and in clusters of molecules that mimic the crystal structure, field emission scanning electron microscopy, differential scanning calorimetry, and high resolution 1H NMR spectroscopy. The solid state 1H spin-lattice relaxation experiments allow us to observe the temperature dependence of the parameters that characterize methyl group rotation in both compounds and in mixtures of the two compounds. In the mixtures, both types of methyl groups (that is, molecules of 1 and 2) can be observed independently and simultaneously at low temperatures because the solid state 1H spin-lattice relaxation is appropriately described by a double exponential. We have followed the conversion 1 → 2 over periods of two years. The solid state 1H spin-lattice relaxation experiments in pure samples of 1 and 2 indicate that there is a distribution of NMR activation energies for methyl group rotation in 1 but not in 2 and we are able to explain this in terms of the particle sizes seen in the field emission scanning electron microscopy images