413 research outputs found

    Zonal large-eddy simulation of a tip leakage flow

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    The flow induced by the clearance between the tip of an isolated airfoil and an end-plate is investigated numerically, using a zonal approach with large-eddy simulation in the region of interest. The results are analyzed in comparison with available experimental data, presented in a companion paper. The incoming boundary layer and the pressure distribution around the blade are evaluated. The description of the inflow-jet deviation, with an averaged approach, enables to represent the proper loading on the airfoil. Also, particular attention is paid to the powerful tipleakage vortex. The vortex characteristics are investigated using specific functions to locate its center and quantify its width. Overall, good results are obtained for the flow statistics and spectra. Furthermore, a very good description of the far-field pressure is achieved using the acoustic analogy, and the results confirm that the tip-flow essentially radiates in the central frequency range (0.7 kHz, 7 kHz)

    Wavelet Analysis of a Blade Tip-Leakage Flow

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    The secondary flow generated by the clearance between an isolated airfoil tip and an end-plate is analyzed by means of a zonal large-eddy simulation, in comparison with available experimental data. The flow around the tip clearance is described with full large-eddy simulation, while Reynolds-averaged Navier-Stokes is employed in the rest of the computational domain in order to limit the computational cost. The various analyses of the flow characteristics (mean velocities, Reynolds stresses, spectra) show a very good agreement between the experiment and the simulation. Furthermore, a detailed analysis is carried out from the numerical results. The flow separations on the blade tip are related with the leakage distribution along the chord, which generates an intense tip-leakage vortex on the suction side. Finally, a hump in the pressure spectra at tip is investigated by means of a wavelet conditional average, and related to the unsteadiness of the aft tip separatio

    Adjusting the cation and anion nature in ionic liquids used for the growth control of nanoparticles of organic conductors

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    Ionic liquids are used for controlling the growth of organic conductors as nanoparticles. We review the conditions of preparation of nanoparticles of conductors derived from tetrathiafulvalene (TTF), tetramethyltetraselenafulvalene (TMTSF) and bis-ethylenedithiotetrathiafulvalene (BEDT-TTF). They are prepared by electrocrystallization using an ionic liquid supporting electrolyte in which the cation plays the role of growth controller and the anion enters the composition of the expected organic conductor. Stable suspensions of nanoparticles are obtained in one case, a valuable characteristic for potential applications in electronic devices

    The BRCT domain of mammalian Rev1 is involved in regulating DNA translesion synthesis

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    Rev1 is a deoxycytidyl transferase associated with DNA translesion synthesis (TLS). In addition to its catalytic domain, Rev1 possesses a so-called BRCA1 C-terminal (BRCT) domain. Here, we describe cells and mice containing a targeted deletion of this domain. Rev1(B/B) mice are healthy, fertile and display normal somatic hypermutation. Rev1(B/B) cells display an elevated spontaneous frequency of intragenic deletions at Hprt. In addition, these cells were sensitized to exogenous DNA damages. Ultraviolet-C (UV-C) light induced a delayed progression through late S and G2 phases of the cell cycle and many chromatid aberrations, specifically in a subset of mutant cells, but not enhanced sister chromatid exchanges (SCE). UV-C-induced mutagenesis was reduced and mutations at thymidine–thymidine dimers were absent in Rev1(B/B) cells, the opposite phenotype of UV-C-exposed cells from XP-V patients, lacking TLS polymerase η. This suggests that the enhanced UV-induced mutagenesis in XP-V patients may depend on error-prone Rev1-dependent TLS. Together, these data indicate a regulatory role of the Rev1 BRCT domain in TLS of a limited spectrum of endogenous and exogenous nucleotide damages during a defined phase of the cell cycle

    Identification of Highly Pathogenic Microorganisms by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: Results of an Interlaboratory Ring Trial

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    In the case of a release of highly pathogenic bacteria (HPB), there is an urgent need for rapid, accurate, and reliable diagnostics. MALDI-TOF mass spectrometry is a rapid, accurate, and relatively inexpensive technique that is becoming increasingly important in microbiological diagnostics to complement classical microbiology, PCR, and genotyping of HPB. In the present study, the results of a joint exercise with 11 partner institutions from nine European countries are presented. In this exercise, 10 distinct microbial samples, among them five HPB, Bacillus anthracis, Brucella canis, Burkholderia mallei, Burkholderia pseudomallei, and Yersinia pestis, were characterized under blinded conditions. Microbial strains were inactivated by high-dose gamma irradiation before shipment. Preparatory investigations ensured that this type of inactivation induced only subtle spectral changes with negligible influence on the quality of the diagnosis. Furthermore, pilot tests on nonpathogenic strains were systematically conducted to ensure the suitability of sample preparation and to optimize and standardize the workflow for microbial identification. The analysis of the microbial mass spectra was carried out by the individual laboratories on the basis of spectral libraries available on site. All mass spectra were also tested against an in-house HPB library at the Robert Koch Institute (RKI). The averaged identification accuracy was 77% in the first case and improved to >93% when the spectral diagnoses were obtained on the basis of the RKI library. The compilation of complete and comprehensive databases with spectra from a broad strain collection is therefore considered of paramount importance for accurate microbial identification

    Four Molecular Superconductors Isolated as Nanoparticles

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    (TMTSF)2PF6 and (TMTSF)2ClO4 Bechgaard salts, (BEDT‐TTF)2I3, and TTF[Ni(dmit)2]2 [TMTSF = tetramethyltetraselenafulvalene; BEDT‐TTF = bis(ethylenedithio)tetrathiafulvalene; TTF = tetrathiafulvalene; dmit = 1,3‐dithiole‐2‐thione‐4,5‐dithiolate] are among the most popular molecular superconductors. They are grown as nanoparticles that exhibit properties in agreement with those of the bulk. The shape, size, and homogeneity of particles depend on the stabilizing agent and synthesis conditions. We report on the more recent conditions investigated (i) to produce nanoparticles of 35 nm for ÎČ‐(BEDT‐TTF)2I3, (ii) to reduce the particle size to 10–15 nm for TTF[Ni(dmit)2]2 and 3–5 nm for (TMTSF)2ClO4, and (iii) to improve the growth duration from days to one hour for (TMTSF)2PF6. Finally, we report evidence of superconductivity in (TMTSF)2ClO4 particles
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