Energy consumption of milking pump controlled by frequency convertor during milking cycle

ArticleThe article deals with selected parameters affecting the energy consumption of a vacuum pump in a milking system during the whole milking cycle in variants with and without regulation by a frequency convertor. When put into practice, the latest research of creation, control and stabilization of vacuum in milking devices allows dairy farmers to obtain a vacuum system that ensures maximum stability of milking pressure, which is a basic requirement affecting the health of dairy cows. The choice of vacuum system prioritizes in particular high performance, maximum operational reliability, minimum maintenance, long service life, environmental friendliness and economy. The vacuum pump was a Roots vacuum pump with a rotary piston which is typical for this use. Use of a frequency convertor significantly affected the efficiency of this pump for control of vacuum pressure level and pump performance by varying the rotation frequency according to the actual airflow requirement. Using this control system, only as much vacuum pressure is produced as necessary. By measurement of an experimental setup, it was found that the average power requirement of a setup with a control valve was 3.8 kW compared to 1.7 kW in the case of the variant with frequency convertor. Measurements and calculations have shown that this system is capable of saving more than 50% of electric energy

Influence of Camera Setting on Vehicle-to-Vehicle VLC Employing Undersampled Phase Shift On-Off Keying

This paper focuses on the performance analysis of a camera based vehicle-to-vehicle visible light communication system employing undersampled phase shift on-off keying modulation under interference scenario. Two Nissan Qashqai front lights with daylight running light emitting diodes based lamps are used for communications. The bit error rate (BER) performance of the proposed system is experimentally measured for a transmission span up to 24m focusing mostly on the side interference due to reflections. Based on experimental data we demonstrate reduction of the system performance due to the side reflection and illumination of the detector by other light sources which has to taken into account during further data processing. We provide with further statistics for particular shuter speed and transmitter power setting and discus BER improvement especially to meet FEC via the method of adaptive region of interest

Substrate protein folds while it is bound to the ATP-independent chaperone Spy

Chaperones assist the folding of many proteins in the cell. While the most well studied chaperones use cycles of ATP binding and hydrolysis to assist protein folding, a number of chaperones have been identified that promote protein folding in the absence of highenergy cofactors. Precisely how ATP-independent chaperones accomplish this feat is unclear. Here we have characterized the kinetic mechanism of substrate folding by the small, ATP-independent chaperone, Spy. Spy rapidly associates with its substrate, Immunity protein 7 (Im7), eliminating its potential for aggregation. Remarkably, Spy then allows Im7 to fully fold into its native state while remaining bound to the surface of the chaperone. These results establish a potentially widespread mechanism whereby ATP-independent chaperones can assist in protein refolding. They also provide compelling evidence that substrate proteins can fold while continuously bound to a chaperone

Macroeconomic policy interaction: State dependency and implications for financial stability in UK: A systemic review

The association between economic and financial stabilities and influence of macroeconomic policies on the financial sector creates scope of active policy role in financial stability. As a contribution to the existing body of knowledge, this study has analysed the implications of macroeconomic policy interaction/coordination for financial stability, proxied by financial assets, i.e. equity and bonds price oscillation. The critical review and analysis of the existing literature on the subject suggests that there is also ample evidence of interdependence between monetary and fiscal policies and this interrelation necessitates coordination between them for the sake of financial stability. There is also a case for analysing the symmetry of financial markets responses to macroeconomic policy interaction. On methodological and empirical grounds, it is vital to test the robustness of policy recommendations to overcome the limitation of a single empirical approach (Jeffrey–Lindley’s paradox). Hence, the Frequentist and Bayesian approaches should be used in commentary manner. The policy interaction and optimal policy combination should also be analysed in the context of institutional design and major financial events to gain insight into the implications of policy interaction in the periods of stable economic and financial environments as well as period of financial and economic distress

Chaperones convert the energy from ATP into the nonequilibrium stabilization of native proteins.

During and after protein translation, molecular chaperones require ATP hydrolysis to favor the native folding of their substrates and, under stress, to avoid aggregation and revert misfolding. Why do some chaperones need ATP, and what are the consequences of the energy contributed by the ATPase cycle? Here, we used biochemical assays and physical modeling to show that the bacterial chaperones GroEL (Hsp60) and DnaK (Hsp70) both use part of the energy from ATP hydrolysis to restore the native state of their substrates, even under denaturing conditions in which the native state is thermodynamically unstable. Consistently with thermodynamics, upon exhaustion of ATP, the metastable native chaperone products spontaneously revert to their equilibrium non-native states. In the presence of ATPase chaperones, some proteins may thus behave as open ATP-driven, nonequilibrium systems whose fate is only partially determined by equilibrium thermodynamics

Compatibility of Aprotic Electrolytes with Negative Electrode Materials in Lithium-ion Batteries

This short research presents an introduction to the fire safety of Lithium-ion batteries. Nowadays, as the most weak part of aprotic battery is its aprotic electrolyte. The aprotic electrolyte (commonly the mixture of aprotic solvents) that is used in Lithium-ion batteries have got low flash point with poor thermal stability. This paper describes using sulfolane as an aprotic solvent in Lithium-ion battery. Measurements were focused on performance, cyclability and stability of aprotic electrolyte with relation of graphite material as a negative electrode. Like a possible substitution of graphite, the LTO material was used and tested. Lithium Titanate Oxide (LTO) seems to be promising negative electrode material for Lithium-ion batteries.Fil: Libich, Jiri. Brno University of Technology. The Faculty of Electrical Engineering and Communication; República ChecaFil: Máca, J.. Brno University of Technology. The Faculty of Electrical Engineering and Communication; República ChecaFil: Sedlaíková, M.. Brno University of Technology. The Faculty of Electrical Engineering and Communication; República ChecaFil: Vondrák, J.. Brno University of Technology. The Faculty of Electrical Engineering and Communication; República ChecaFil: Visintin, Arnaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de la Plata; Argentina. Brno University of Technology. The Faculty of Electrical Engineering and Communication; República Chec

Correction of Thermal Deviations of Fabry-Perot Resonator Based Measurements of Specific Gases in Millimeter Wave Bands

Due to the thermal expansivity of the material used in the Fabry-Perot resonator mirrors, the resonator cavity length can change and this might therefore have an impact on the resonant frequency during high-resolution spectroscopy measurements. Based on measurements and simulations, this paper discusses the influences of temperature on the precise determination of gas attenuation measured in a Fabry-Perot resonator. Several measures to mitigate such influence and to correct the measured results were tested. A correction method for the measured data was proposed

Binding of the proline-rich segment of Myelin Basic Protein to SH3-domains – spectroscopic, microarray and modelling studies of ligand conformation and effects of post-translational modifications

Myelin basic protein (MBP) is a multifunctional protein involved in maintaining the stability and integrity of the myelin sheath by a variety of interactions with membranes and with cytoskeletal and other proteins. A central segment of MBP is highly conserved in mammals and consists of a membrane surface-associated amphipathic -helix, immediately followed by a proline-rich segment that we hypothesize is an SH3 ligand. We show by circular dichroic spectroscopy that this proline-rich segment forms a polyproline type II helix in vitro under physiological conditions and that phosphorylation at a constituent threonyl residue has a stabilizing effect on its conformation. Using SH3 domain microarrays, we observe that the unmodified recombinant murine 18.5 kDa MBP isoform (rmC1 component) binds the following SH3 domains: Yes1 > PSD95 > cortactin = PexD = Abl = Fyn = c-Src = Itk in order of decreasing affinity. A quasi-deiminated form of the protein (rmC8) binds the SH3 domains Yes1 > Fyn > cortactin = c-Src > PexD = Abl. Phosphorylation of rmC1 at 1-2 threonines within the proline-rich segment by mitogen-activated protein kinase in vitro has no effect on the binding specificity to the SH3 domains on the array. An SH3 domain of chicken Fyn is also demonstrated to bind to lipid membrane-associated C1, phosphorylated C1, and rmC8. Molecular docking simulations of the interaction of the putative SH3 ligand of classic MBP with the human Fyn SH3 domain indicate that the strength of the interaction is of the same order of magnitude as with calmodulin and that the molecular recognition and association is mediated by some weak CH··· interactions between the ligand prolyl residues and the aromatic ones of the SH3 binding site. One such interaction is well-conserved and involves the stacking of an MBP-peptide prolyl and an SH3 domain tryptophanyl residue, as in most other SH3-ligand complexes. Lysyl and arginyl residues in the peptide canonically interact via salt bridges and cation- interactions with negatively charged and aromatic residues in the SH3 domain binding site. Posttranslational modifications (phosphorylation or methylation) of the ligand cause noticeable shifts in the conformation of the flexible peptide and its side chains but do not predict any major inhibition of the binding beyond somewhat less favorable interactions for peptides with phosphorylated seryl or threonyl residues