Retrograde Accretion and Merging Supermassive Black Holes

We investigate whether a circumbinary gas disc can coalesce a supermassive black hole binary system in the centre of a galaxy. This is known to be problematic for a prograde disc. We show that in contrast, interaction with a retrograde circumbinary disc is considerably more effective in shrinking the binary because there are no orbital resonances. The binary directly absorbs negative angular momentum from the circumbinary disc by capturing gas into a disc around the secondary black hole, or discs around both holes if the binary mass ratio is close to unity. In many cases the binary orbit becomes eccentric, shortening the pericentre distance as the eccentricity grows. In all cases the binary coalesces once it has absorbed the angular momentum of a gas mass comparable to that of the secondary black hole. Importantly, this conclusion is unaffected even if the gas inflow rate through the disc is formally super--Eddington for either hole. The coalescence timescale is therefore always $\sim M_2/\dot M$, where $M_2$ is the secondary black hole mass and $\dot M$ the inflow rate through the circumbinary disc.Comment: 8 pages, 4 figures. Accepted for publication in MNRAS. Movies of the simulations can be found at: http://www.astro.le.ac.uk/users/cjn12/RetroBinaryMovies.htm

In-Situ Cure Monitoring of the Immidization Reaction of PMR-15

Glass fiber reinforced polymer composites are becoming widely used in industry. With this increase in production, an in-situ method of quality control for the curing of the polymer is desirable. This would allow for the production of high-quality parts having more uniform properties.' Recently, in-situ fiber optic monitoring of polymer curing has primarily focused on epoxy resins and has been performed by Raman or fluorescence methods. In addition, some infrared (IR) investigations have been performed using transmission or ATR cells. An alternate IR approach involves using optical fibers as a sensor by utilizing evanescent wave spectroscopy

Lack of correlation between MYCN expression and the Warburg effect in neuroblastoma cell lines

<p>Abstract</p> <p>Background</p> <p>Many cancers preferentially meet their energy requirements through the glycolytic pathway rather than via the more efficient oxidative phosphorylation pathway. It is thought that this is an important adaptation in cancer malignancy. We investigated whether use of glycolysis for energy production even in the presence of oxygen (known as the Warburg effect) varied between neuroblastoma cell lines with or without <it>MYCN </it>amplification (a key indicator of poor disease outcome in neuroblastoma).</p> <p>Methods</p> <p>We examined ATP and lactate production, oxygen consumption and mitochondrial energisation status for three neuroblastoma cell lines with varying degrees of <it>MYCN </it>amplification and MYCN expression.</p> <p>Results</p> <p>We found no correlation between MYCN expression and the Warburg effect in the cell lines investigated.</p> <p>Conclusion</p> <p>Our results suggest preferential use of glycolysis for energy production and MYCN expression may be independent markers of neuroblastoma malignancy <it>in vitro </it>if not <it>in vivo</it>.</p

Comparison between SELEX and single-step selection for the identification of bivalent aptamers for thrombin

The identification of bivalent aptamers for thrombin by SELEX and single-step selection are compared using next generation (massively parallel) sequencing and motif finding informatics. Results show that similar aptamers are identified by both methods

Turbulent Linewidths as a Diagnostic of Self-Gravity in Protostellar Discs

We use smoothed particle hydrodynamics simulations of massive protostellar discs to investigate the predicted broadening of molecular lines from discs in which self-gravity is the dominant source of angular momentum transport. The simulations include radiative transfer, and span a range of disc-to-star mass ratios between 0.25 and 1.5. Subtracting off the mean azimuthal flow velocity, we compute the distribution of the in-plane and perpendicular peculiar velocity due to large scale structure and turbulence induced by self-gravity. For the lower mass discs, we show that the characteristic peculiar velocities scale with the square root of the effective turbulent viscosity parameter, as expected from local turbulent-disc theory. The derived velocities are anisotropic, with substantially larger in-plane than perpendicular values. As the disc mass is increased, the validity of the locally determined turbulence approximation breaks down, and this is accompanied by anomalously large in-plane broadening. There is also a high variance due to the importance of low-m spiral modes. For low-mass discs, the magnitude of in-plane broadening is, to leading order, equal to the predictions from local disc theory and cannot constrain the source of turbulence. However, combining our results with prior evaluations of turbulent broadening expected in discs where the magnetorotational instability (MRI) is active, we argue that self-gravity may be distinguishable from the MRI in these systems if it is possible to measure the anisotropy of the peculiar velocity field with disc inclination. Furthermore, for large mass discs, the dominant contribution of large-scale modes is a distinguishing characteristic of self-gravitating turbulence versus MRI driven turbulence.Comment: 8 pages, 13 figures, accepted for publication in MNRA

Investigation of Van Gogh-like 2 mRNA Regulation and Localisation in Response to Nociception in the Brain of Adult Common Carp (Cyprinus carpio)

The Van Gogh-like 2 (vangl2) gene is typically associated with planar cell polarity pathways, which is essential for correct orientation of epithelial cells during development. The encoded protein of this gene is a transmembrane protein and is highly conserved through evolution. Van Gogh-like 2 was selected for further study on the basis of consistent regulation after a nociceptive stimulus in adult common carp and rainbow trout in a microarray study. An in situ hybridisation was conducted in the brain of mature common carp (Cyprinus carpio), 1.5 and 3 h after a nociceptive stimulus comprising of an acetic acid injection to the lips of the fish and compared with a saline-injected control. The vangl2 gene was expressed in all brain regions, and particularly intensely in neurons of the telencephalon and in ependymal cells. In the cerebellum, a greater number (P = 0.018) of Purkinje cells expressed vangl2 after nociception (n=7) compared with controls (n = 5). This regulation opens the possibility that vangl2 is involved in nociceptive processing in the adult fish brain and may be a novel target for central nociception in vertebrates

Characterising the Gravitational Instability in Cooling Accretion Discs

We perform numerical analyses of the structure induced by gravitational instabilities in cooling gaseous accretion discs. For low enough cooling rates a quasi-steady configuration is reached, with the instability saturating at a finite amplitude in a marginally stable disc. We find that the saturation amplitude scales with the inverse square root of the cooling parameter beta = t_cool / t_dyn, which indicates that the heating rate induced by the instability is proportional to the energy density of the induced density waves. We find that at saturation the energy dissipated per dynamical time by weak shocks due is of the order of 20 per cent of the wave energy. From Fourier analysis of the disc structure we find that while the azimuthal wavenumber is roughly constant with radius, the mean radial wavenumber increases with radius, with the dominant mode corresponding to the locally most unstable wavelength. We demonstrate that the density waves excited in relatively low mass discs are always close to co-rotation, deviating from it by approximately 10 per cent. This can be understood in terms of the flow Doppler-shifted phase Mach number -- the pattern speed self-adjusts so that the flow into spiral arms is always sonic. This has profound effects on the degree to which transport through self-gravity can be modelled as a viscous process. Our results thus provide (a) a detailed description of how the self-regulation mechanism is established for low cooling rates, (b) a clarification of the conditions required for describing the transport induced by self-gravity through an effective viscosity, (c) an estimate of the maximum amplitude of the density perturbation before fragmentation occurs, and (d) a simple recipe to estimate the density perturbation in different thermal regimes.Comment: 16 pages, 22 figures. Accepted for publication in MNRAS 11 November 200

Convergence of SPH simulations of self-gravitating accretion discs: Sensitivity to the implementation of radiative cooling

Recent simulations of self-gravitating accretion discs, carried out using a three-dimensional Smoothed Particle Hydrodynamics (SPH) code by Meru and Bate, have been interpreted as implying that three-dimensional global discs fragment much more easily than would be expected from a two-dimensional local model. Subsequently, global and local two-dimensional models have been shown to display similar fragmentation properties, leaving it unclear whether the three-dimensional results reflect a physical effect or a numerical problem associated with the treatment of cooling or artificial viscosity in SPH. Here, we study how fragmentation of self-gravitating disc flows in SPH depends upon the implementation of cooling. We run disc simulations that compare a simple cooling scheme, in which each particle loses energy based upon its internal energy per unit mass, with a method in which the cooling is derived from a smoothed internal energy density field. For the simple per particle cooling scheme, we find a significant increase in the minimum cooling time scale for fragmentation with increasing resolution, matching previous results. Switching to smoothed cooling, however, results in lower critical cooling time scales, and tentative evidence for convergence at the highest spatial resolution tested. We conclude that precision studies of fragmentation using SPH require careful consideration of how cooling (and, probably, artificial viscosity) is implemented, and that the apparent non-convergence of the fragmentation boundary seen in prior simulations is likely a numerical effect. In real discs, where cooling is physically smoothed by radiative transfer effects, the fragmentation boundary is probably displaced from the two-dimensional value by a factor that is only of the order of unity.Comment: 9 pages, 11 figures, MNRAS in pres