Mirroring E-service for Brick and Mortar Retail: An Assessment and Survey

The digital transformation increasingly impacts the competitive retail market structure in favor of e-commerce and digital business models, while many Brick and Mortar (BaM) retailers are struggling to meet customers’ expectations. Supported by the customer adaption of e-commerce and digital technologies, this paper applies the lens of channel complementary theory to BaM. We examine, which e-service touchpoints from e-commerce can be transferred to the physical servicescape of BaM retail to complement customer journeys. Drawing from the dominant design theory, we first assess leading e-commerce solutions to identify dominant e-service touchpoints, which are then mirrored for their application in BaM retail. Second, we surveyed 250 shoppers to elicit the likeliness of use regarding these touchpoints. Our results provide a foundation for both academia and retail to advance the knowledge of relevant e-service touchpoints in BaM

Machbarkeitsstudie zu den IT-technischen Anforderungen einer weitergehenden Digitalisierung im Bereich Soziale Sicherheit

Ausgehend von der erfolgten Einführung des EESSI-Verfahrens ist es Aufgabe dieser Kurzstudie, weitere Digitalisierungspotentiale im Bereich der sozialen Sicherheit in Deutschland zu identifizieren. Dabei wurde eine mittel- bis langfristige Perspektive eingenommen, um Potentiale der Digitalisierung in Form von aktuellen und innovativen Konzepten und Technologien aufzuzeigen. Die Identifizierung dieser Potentiale erfolgte auf Basis von semi-strukturierten Interviews mit Experten aus der Praxis, die sowohl mit dem EESSI-Verfahren als auch generell mit den Themen der Digitalisierung im Bereich der Systeme der sozialen Sicherheit vertraut sind. Dabei konnten im Verlauf der Studie sowohl regionale, überregionale und bundesweite Organisationen konsultiert werden, um ein breites Bild der aktuellen Entwicklungen und Herausforderungen im Bereich der Digitalisierung abzuleiten. Neben dem EESSI-Verfahren wurde allgemein über die aktuellen Potentiale im Zuge der Digitalisierung diskutiert. Zur Adressierung der Herausforderungen wurden Digitalisierungspotentiale identifiziert, die aus einer Nutzenperspektive sowohl für die Bürgerinnen und Bürger als auch für die Anwender beim Sozialversicherungsträger betrachtet wurden. Zusätzlich wurde, sofern es möglich war, diskutiert, inwiefern die aufgezeigten Digitalisierungswege Betrug und Missbrauch in den Systemen der sozialen Sicherheit eindämmen oder ganz verhindern können. Die insgesamt acht aus den diskutierten Herausforderungen abgeleiteten Digitalisierungspotentiale wurden durch dedizierte Handlungsempfehlungen operationalisiert. Diese Digitalisierungspotentiale zeigen erste Wege auf, die im Rahmen einer zukünftig fortschreitenden Digitalisierung im Bereich der sozialen Sicherheit und der Sozialversicherungen bedacht werden sollten.Based on the recent introduction of EESSI, the task of this study is to identify further digitalization potential for the Systems of Social Security in Germany. In doing so, a medium to long-term perspective was adopted in order to highlight the potential of digitalization in the form of and on the basis of current and innovative concepts and technologies. The identification of the potential in different areas was based on semi-structured interviews with experts who are familiar with EESSI and general topics linked to the digitalization of the Social Security system in Germany. During this study, it was possible to consult regional and federal organizations in order to allow for the development of a broad picture of the current challenges concerning ongoing and future digitalization efforts. Next to EESSI, the conducted interviews discussed general challenges linked to digitalization processes. Based on these challenges, the digitalization potential was derived in different application areas. In doing so, multiple perspectives have been applied, to discuss not only potential benefits for the employees and the social security institutions, but also the benefits for citizens. If applicable, the derived potential was discussed in terms of its ability to prevent fraud and misuse within the systems of Social Security in Germany. In total, eight potential digitization approaches were derived and operationalized in the form of dedicated recommendations for action. They may show first paths that should be considered in the context of the future digitalization actions in the area of Social Security in Germany

Human Practice. Digital Ecologies. Our Future. : 14. Internationale Tagung Wirtschaftsinformatik (WI 2019) : Tagungsband

Erschienen bei: universi - Universitätsverlag Siegen. - ISBN: 978-3-96182-063-4Aus dem Inhalt: Track 1: Produktion & Cyber-Physische Systeme Requirements and a Meta Model for Exchanging Additive Manufacturing Capacities Service Systems, Smart Service Systems and Cyber- Physical Systems—What’s the difference? Towards a Unified Terminology Developing an Industrial IoT Platform – Trade-off between Horizontal and Vertical Approaches Machine Learning und Complex Event Processing: Effiziente Echtzeitauswertung am Beispiel Smart Factory Sensor retrofit for a coffee machine as condition monitoring and predictive maintenance use case Stakeholder-Analyse zum Einsatz IIoT-basierter Frischeinformationen in der Lebensmittelindustrie Towards a Framework for Predictive Maintenance Strategies in Mechanical Engineering - A Method-Oriented Literature Analysis Development of a matching platform for the requirement-oriented selection of cyber physical systems for SMEs Track 2: Logistic Analytics An Empirical Study of Customers’ Behavioral Intention to Use Ridepooling Services – An Extension of the Technology Acceptance Model Modeling Delay Propagation and Transmission in Railway Networks What is the impact of company specific adjustments on the acceptance and diffusion of logistic standards? Robust Route Planning in Intermodal Urban Traffic Track 3: Unternehmensmodellierung & Informationssystemgestaltung (Enterprise Modelling & Information Systems Design) Work System Modeling Method with Different Levels of Specificity and Rigor for Different Stakeholder Purposes Resolving Inconsistencies in Declarative Process Models based on Culpability Measurement Strategic Analysis in the Realm of Enterprise Modeling – On the Example of Blockchain-Based Initiatives for the Electricity Sector Zwischenbetriebliche Integration in der Möbelbranche: Konfigurationen und Einflussfaktoren Novices’ Quality Perceptions and the Acceptance of Process Modeling Grammars Entwicklung einer Definition für Social Business Objects (SBO) zur Modellierung von Unternehmensinformationen Designing a Reference Model for Digital Product Configurators Terminology for Evolving Design Artifacts Business Role-Object Specification: A Language for Behavior-aware Structural Modeling of Business Objects Generating Smart Glasses-based Information Systems with BPMN4SGA: A BPMN Extension for Smart Glasses Applications Using Blockchain in Peer-to-Peer Carsharing to Build Trust in the Sharing Economy Testing in Big Data: An Architecture Pattern for a Development Environment for Innovative, Integrated and Robust Applications Track 4: Lern- und Wissensmanagement (e-Learning and Knowledge Management) eGovernment Competences revisited – A Literature Review on necessary Competences in a Digitalized Public Sector Say Hello to Your New Automated Tutor – A Structured Literature Review on Pedagogical Conversational Agents Teaching the Digital Transformation of Business Processes: Design of a Simulation Game for Information Systems Education Conceptualizing Immersion for Individual Learning in Virtual Reality Designing a Flipped Classroom Course – a Process Model The Influence of Risk-Taking on Knowledge Exchange and Combination Gamified Feedback durch Avatare im Mobile Learning Alexa, Can You Help Me Solve That Problem? - Understanding the Value of Smart Personal Assistants as Tutors for Complex Problem Tasks Track 5: Data Science & Business Analytics Matching with Bundle Preferences: Tradeoff between Fairness and Truthfulness Applied image recognition: guidelines for using deep learning models in practice Yield Prognosis for the Agrarian Management of Vineyards using Deep Learning for Object Counting Reading Between the Lines of Qualitative Data – How to Detect Hidden Structure Based on Codes Online Auctions with Dual-Threshold Algorithms: An Experimental Study and Practical Evaluation Design Features of Non-Financial Reward Programs for Online Reviews: Evaluation based on Google Maps Data Topic Embeddings – A New Approach to Classify Very Short Documents Based on Predefined Topics Leveraging Unstructured Image Data for Product Quality Improvement Decision Support for Real Estate Investors: Improving Real Estate Valuation with 3D City Models and Points of Interest Knowledge Discovery from CVs: A Topic Modeling Procedure Online Product Descriptions – Boost for your Sales? Entscheidungsunterstützung durch historienbasierte Dienstreihenfolgeplanung mit Pattern A Semi-Automated Approach for Generating Online Review Templates Machine Learning goes Measure Management: Leveraging Anomaly Detection and Parts Search to Improve Product-Cost Optimization Bedeutung von Predictive Analytics für den theoretischen Erkenntnisgewinn in der IS-Forschung Track 6: Digitale Transformation und Dienstleistungen Heuristic Theorizing in Software Development: Deriving Design Principles for Smart Glasses-based Systems Mirroring E-service for Brick and Mortar Retail: An Assessment and Survey Taxonomy of Digital Platforms: A Platform Architecture Perspective Value of Star Players in the Digital Age Local Shopping Platforms – Harnessing Locational Advantages for the Digital Transformation of Local Retail Outlets: A Content Analysis A Socio-Technical Approach to Manage Analytics-as-a-Service – Results of an Action Design Research Project Characterizing Approaches to Digital Transformation: Development of a Taxonomy of Digital Units Expectations vs. Reality – Benefits of Smart Services in the Field of Tension between Industry and Science Innovation Networks and Digital Innovation: How Organizations Use Innovation Networks in a Digitized Environment Characterising Social Reading Platforms— A Taxonomy-Based Approach to Structure the Field Less Complex than Expected – What Really Drives IT Consulting Value Modularity Canvas – A Framework for Visualizing Potentials of Service Modularity Towards a Conceptualization of Capabilities for Innovating Business Models in the Industrial Internet of Things A Taxonomy of Barriers to Digital Transformation Ambidexterity in Service Innovation Research: A Systematic Literature Review Design and success factors of an online solution for cross-pillar pension information Track 7: IT-Management und -Strategie A Frugal Support Structure for New Software Implementations in SMEs How to Structure a Company-wide Adoption of Big Data Analytics The Changing Roles of Innovation Actors and Organizational Antecedents in the Digital Age Bewertung des Kundennutzens von Chatbots für den Einsatz im Servicedesk Understanding the Benefits of Agile Software Development in Regulated Environments Are Employees Following the Rules? On the Effectiveness of IT Consumerization Policies Agile and Attached: The Impact of Agile Practices on Agile Team Members’ Affective Organisational Commitment The Complexity Trap – Limits of IT Flexibility for Supporting Organizational Agility in Decentralized Organizations Platform Openness: A Systematic Literature Review and Avenues for Future Research Competence, Fashion and the Case of Blockchain The Digital Platform Otto.de: A Case Study of Growth, Complexity, and Generativity Track 8: eHealth & alternde Gesellschaft Security and Privacy of Personal Health Records in Cloud Computing Environments – An Experimental Exploration of the Impact of Storage Solutions and Data Breaches Patientenintegration durch Pfadsysteme Digitalisierung in der Stressprävention – eine qualitative Interviewstudie zu Nutzenpotenzialen User Dynamics in Mental Health Forums – A Sentiment Analysis Perspective Intent and the Use of Wearables in the Workplace – A Model Development Understanding Patient Pathways in the Context of Integrated Health Care Services - Implications from a Scoping Review Understanding the Habitual Use of Wearable Activity Trackers On the Fit in Fitness Apps: Studying the Interaction of Motivational Affordances and Users’ Goal Orientations in Affecting the Benefits Gained Gamification in Health Behavior Change Support Systems - A Synthesis of Unintended Side Effects Investigating the Influence of Information Incongruity on Trust-Relations within Trilateral Healthcare Settings Track 9: Krisen- und Kontinuitätsmanagement Potentiale von IKT beim Ausfall kritischer Infrastrukturen: Erwartungen, Informationsgewinnung und Mediennutzung der Zivilbevölkerung in Deutschland Fake News Perception in Germany: A Representative Study of People’s Attitudes and Approaches to Counteract Disinformation Analyzing the Potential of Graphical Building Information for Fire Emergency Responses: Findings from a Controlled Experiment Track 10: Human-Computer Interaction Towards a Taxonomy of Platforms for Conversational Agent Design Measuring Service Encounter Satisfaction with Customer Service Chatbots using Sentiment Analysis Self-Tracking and Gamification: Analyzing the Interplay of Motivations, Usage and Motivation Fulfillment Erfolgsfaktoren von Augmented-Reality-Applikationen: Analyse von Nutzerrezensionen mit dem Review-Mining-Verfahren Designing Dynamic Decision Support for Electronic Requirements Negotiations Who is Stressed by Using ICTs? A Qualitative Comparison Analysis with the Big Five Personality Traits to Understand Technostress Walking the Middle Path: How Medium Trade-Off Exposure Leads to Higher Consumer Satisfaction in Recommender Agents Theory-Based Affordances of Utilitarian, Hedonic and Dual-Purposed Technologies: A Literature Review Eliciting Customer Preferences for Shopping Companion Apps: A Service Quality Approach The Role of Early User Participation in Discovering Software – A Case Study from the Context of Smart Glasses The Fluidity of the Self-Concept as a Framework to Explain the Motivation to Play Video Games Heart over Heels? An Empirical Analysis of the Relationship between Emotions and Review Helpfulness for Experience and Credence Goods Track 11: Information Security and Information Privacy Unfolding Concerns about Augmented Reality Technologies: A Qualitative Analysis of User Perceptions To (Psychologically) Own Data is to Protect Data: How Psychological Ownership Determines Protective Behavior in a Work and Private Context Understanding Data Protection Regulations from a Data Management Perspective: A Capability-Based Approach to EU-GDPR On the Difficulties of Incentivizing Online Privacy through Transparency: A Qualitative Survey of the German Health Insurance Market What is Your Selfie Worth? A Field Study on Individuals’ Valuation of Personal Data Justification of Mass Surveillance: A Quantitative Study An Exploratory Study of Risk Perception for Data Disclosure to a Network of Firms Track 12: Umweltinformatik und nachhaltiges Wirtschaften Kommunikationsfäden im Nadelöhr – Fachliche Prozessmodellierung der Nachhaltigkeitskommunikation am Kapitalmarkt Potentiale und Herausforderungen der Materialflusskostenrechnung Computing Incentives for User-Based Relocation in Carsharing Sustainability’s Coming Home: Preliminary Design Principles for the Sustainable Smart District Substitution of hazardous chemical substances using Deep Learning and t-SNE A Hierarchy of DSMLs in Support of Product Life-Cycle Assessment A Survey of Smart Energy Services for Private Households Door-to-Door Mobility Integrators as Keystone Organizations of Smart Ecosystems: Resources and Value Co-Creation – A Literature Review Ein Entscheidungsunterstützungssystem zur ökonomischen Bewertung von Mieterstrom auf Basis der Clusteranalyse Discovering Blockchain for Sustainable Product-Service Systems to enhance the Circular Economy Digitale Rückverfolgbarkeit von Lebensmitteln: Eine verbraucherinformatische Studie Umweltbewusstsein durch audiovisuelles Content Marketing? Eine experimentelle Untersuchung zur Konsumentenbewertung nachhaltiger Smartphones Towards Predictive Energy Management in Information Systems: A Research Proposal A Web Browser-Based Application for Processing and Analyzing Material Flow Models using the MFCA Methodology Track 13: Digital Work - Social, mobile, smart On Conversational Agents in Information Systems Research: Analyzing the Past to Guide Future Work The Potential of Augmented Reality for Improving Occupational First Aid Prevent a Vicious Circle! The Role of Organizational IT-Capability in Attracting IT-affine Applicants Good, Bad, or Both? Conceptualization and Measurement of Ambivalent User Attitudes Towards AI A Case Study on Cross-Hierarchical Communication in Digital Work Environments ‘Show Me Your People Skills’ - Employing CEO Branding for Corporate Reputation Management in Social Media A Multiorganisational Study of the Drivers and Barriers of Enterprise Collaboration Systems-Enabled Change The More the Merrier? The Effect of Size of Core Team Subgroups on Success of Open Source Projects The Impact of Anthropomorphic and Functional Chatbot Design Features in Enterprise Collaboration Systems on User Acceptance Digital Feedback for Digital Work? Affordances and Constraints of a Feedback App at InsurCorp The Effect of Marker-less Augmented Reality on Task and Learning Performance Antecedents for Cyberloafing – A Literature Review Internal Crowd Work as a Source of Empowerment - An Empirical Analysis of the Perception of Employees in a Crowdtesting Project Track 14: Geschäftsmodelle und digitales Unternehmertum Dividing the ICO Jungle: Extracting and Evaluating Design Archetypes Capturing Value from Data: Exploring Factors Influencing Revenue Model Design for Data-Driven Services Understanding the Role of Data for Innovating Business Models: A System Dynamics Perspective Business Model Innovation and Stakeholder: Exploring Mechanisms and Outcomes of Value Creation and Destruction Business Models for Internet of Things Platforms: Empirical Development of a Taxonomy and Archetypes Revitalizing established Industrial Companies: State of the Art and Success Principles of Digital Corporate Incubators When 1+1 is Greater than 2: Concurrence of Additional Digital and Established Business Models within Companies Special Track 1: Student Track Investigating Personalized Price Discrimination of Textile-, Electronics- and General Stores in German Online Retail From Facets to a Universal Definition – An Analysis of IoT Usage in Retail Is the Technostress Creators Inventory Still an Up-To-Date Measurement Instrument? Results of a Large-Scale Interview Study Application of Media Synchronicity Theory to Creative Tasks in Virtual Teams Using the Example of Design Thinking TrustyTweet: An Indicator-based Browser-Plugin to Assist Users in Dealing with Fake News on Twitter Application of Process Mining Techniques to Support Maintenance-Related Objectives How Voice Can Change Customer Satisfaction: A Comparative Analysis between E-Commerce and Voice Commerce Business Process Compliance and Blockchain: How Does the Ethereum Blockchain Address Challenges of Business Process Compliance? Improving Business Model Configuration through a Question-based Approach The Influence of Situational Factors and Gamification on Intrinsic Motivation and Learning Evaluation von ITSM-Tools für Integration und Management von Cloud-Diensten am Beispiel von ServiceNow How Software Promotes the Integration of Sustainability in Business Process Management Criteria Catalog for Industrial IoT Platforms from the Perspective of the Machine Tool Industry Special Track 3: Demos & Prototyping Privacy-friendly User Location Tracking with Smart Devices: The BeaT Prototype Application-oriented robotics in nursing homes Augmented Reality for Set-up Processe Mixed Reality for supporting Remote-Meetings Gamification zur Motivationssteigerung von Werkern bei der Betriebsdatenerfassung Automatically Extracting and Analyzing Customer Needs from Twitter: A “Needmining” Prototype GaNEsHA: Opportunities for Sustainable Transportation in Smart Cities TUCANA: A platform for using local processing power of edge devices for building data-driven services Demonstrator zur Beschreibung und Visualisierung einer kritischen Infrastruktur Entwicklung einer alltagsnahen persuasiven App zur Bewegungsmotivation für ältere Nutzerinnen und Nutzer A browser-based modeling tool for studying the learning of conceptual modeling based on a multi-modal data collection approach Exergames & Dementia: An interactive System for People with Dementia and their Care-Network Workshops Workshop Ethics and Morality in Business Informatics (Workshop Ethik und Moral in der Wirtschaftsinformatik – EMoWI’19) Model-Based Compliance in Information Systems - Foundations, Case Description and Data Set of the MobIS-Challenge for Students and Doctoral Candidates Report of the Workshop on Concepts and Methods of Identifying Digital Potentials in Information Management Control of Systemic Risks in Global Networks - A Grand Challenge to Information Systems Research Die Mitarbeiter von morgen - Kompetenzen künftiger Mitarbeiter im Bereich Business Analytics Digitaler Konsum: Herausforderungen und Chancen der Verbraucherinformati

Reasons for Failures of Sharing Economy Businesses

The rapid growth of successful sharing economy businesses like Airbnb and Uber poses a threat to traditional value chains. However, many lesser-known sharing platforms have faced serious setbacks and ceased to operate. We have compiled a profile of failed sharing businesses so that others can learn from their mistakes. Our research identified seven common reasons for the failures and four significant differences between failed and successful sharing businesses. Our findings will help managers of aspiring sharing businesses to mitigate the risks of entering the sharing market.Click here for free presentation slides (pdf

Usage of Local Online Platforms in Retail: Insights from retailers\u27 expectations

Many high street retailers currently face unprecedented challenges arising from the competition with online retail sellers and city centers losing their attractiveness to customers. Aiming to retain footfall to their stores, retailers and local service providers are exploring how digital technology, such as digital platforms, can be harnessed to provide new touchpoints. However, little is known about how high street retailers are using digital platforms and, in particular, Local Online Platforms (LOPs), which are restricted to a geographic area, mostly a city. To address this gap, we conduct qualitative research to investigate the usage of LOPs. Interviews with 19 small and medium-sized enterprise (SME) retailers reveal that they will use digital online platforms, preferentially LOPs, as long as entry barriers are kept low in order to acquire new customers, promote loyalty and strengthen local cooperation between SMEs on the high street. We outline our plans to validate our hypotheses with behavioral data in forthcoming interviews and surveys. This research in progress will thus inform the next research efforts aimed to provide practical insights on the design, adoption, and usage of LOPs to benefit both software designers and SME retailers

INDUSTRY DEMAND FOR ANALYTICS: A LONGITUDINAL STUDY

Data analytics has become an important part of companies in industry, leading to an increase in the demand for analytics experts. Data analytics is also a central aspect of trends such as “Big Data”, “Data Science”, “Artificial Intelligence”. As such, for potential analytics professionals to have relevant job skills, educational institutions need to have an up-to-date picture of the demanded job skills. We studied the recent past to better anticipate future developments of analytics job skills through a study based on text mining using job advertisements from 2014 and 2019 summing up to a total of 17,282 advertisements. We investigated how these trends evolved by looking at meaning of words using associated words as well as topics obtained from topic modeling. Our longitudinal study reveals a shift in prevalence and meaning of analytics trends in the industry. It shows a small shift from business oriented job skills towards analytical and technical ones. This finding is accompanied by increasing popularity of “Big Data”, in contrast with the reduced popularity of “Business Intelligence”. Additionally, we observed how “Machine Learning” is becoming more strongly associated with the field of data science itself

Identification of humid periods in the Atacama Desert through hillslope activity established by infrared stimulated luminescence (IRSL) dating

Geological records indicate that the hyper-aridity in the Atacama Desert has prevailed since at least the mid-Miocene, with shorter periods of increased humidity punctuating long-term aridity. The 7-m-high accumulation of colluvial sediments at the Salar Grande (21 degrees S/70 degrees W) studied here provides a key palaeoclimate record to understand hillslope dynamics and its relation to humid periods. While 1 degrees Be surface exposure ages point to long-term surface stability of the flat upslope surface, a combination of humidity-driven soil creep, overland flow and soil creep related to seismic shaking, caused denudation of the hillslope and accumulation of several metres of colluvium over much shorter timescales during the last 130 ka. A robust chronology for the hillslope sediments has been established by using Infrared Stimulated luminescence (pIR-IR225 protocol) on K-feldspar extracts from nine samples collected within the accumulation. A series of tests has been carried out to confirm the suitability of the method. The estimated ages indicate accelerated sedimentation at 35-80 ka, and 100-130 ka, which are interpreted as periods with more humid climate conditions than present. These findings agree with climate variations in the hyper-arid Atacama indicated by the activity of coastal alluvial fans and river catchments

Development of a steep erosional gradient over a short distance in the hyperarid core of the Atacama Desert, northern Chile

Although generally considered to exist under hyperarid conditions over the long term, landscapes in many parts of the Coastal Cordillera of northern Chile have undergone fluvial erosion. Small-scale drainage systems in this mountain range are mostly isolated from river networks and associated processes originating in the Precordillera or the High Andes to the east, thus providing natural laboratories to investigate the interplay between erosion, atmospheric deposition, tectonics and local (micro-) climatic conditions. In this study, we present a set of cosmogenic Be-10 and Al-26 derived, catchment-wide erosion rates along a short (2.5 km) E-W transect on the northern rim of the Rio Loa Canyon in the Coastal Cordillera of northern Chile (latitude 21.4 degrees S). Here, a flat sedimentary gravel surface, which was deposited before the Middle Miocene, becomes increasingly dissected and changes into a badland-like topography to the west. The Be-10 erosion rates increase by approximately an order of magnitude from east to west, reflecting (1) localised tectonic movements, (2) geologically recent base level lowering, (3) time-integrated (micro-) climatic gradients and (4) the presence/absence of gyperete. These findings are corroborated by analysis of geomorphologic parameters, which point towards the presence of two fundamentally different erosional regimes in this small study area. These regimes are sharply delineated along a topographically modest tectonic ridge. To the west, a detachment-limited erosion regime prevails, while in the east a transport-limited regime is dominant. The presence or absence of gyperete, whose prevalence is governed by (micro-) climatic conditions, generally appears to reflect the respective erosional regimes. The erosion rates we infer point to a long-term process of differential drainage evolution in the study area, likely on timescales of millions of years

Bcl-x(L) as prognostic marker and potential therapeutic target in cholangiocarcinoma

Intrahepatic, perihilar, and distal cholangiocarcinoma (iCCA, pCCA, dCCA) are highly malignant tumours with increasing mortality rates due to therapy resistances. Among the mechanisms mediating resistance, overexpression of anti-apoptotic Bcl-2 proteins (Bcl-2, Bcl-x(L), Mcl-1) is particularly important. In this study, we investigated whether antiapoptotic protein patterns are prognostically relevant and potential therapeutic targets in CCA. Bcl-2 proteins were analysed in a pan-cancer cohort from the NCT/DKFZ/DKTK MASTER registry trial (n = 1140, CCA n = 72) via RNA-sequencing and transcriptome-based protein activity interference revealing high ranks of CCA for Bcl-x(L) and Mcl-1. Expression of Bcl-x(L), Mcl-1, and Bcl-2 was assessed in human CCA tissue and cell lines compared with cholangiocytes by immunohistochemistry, immunoblotting, and quantitative-RT-PCR. Immunohistochemistry confirmed the upregulation of Bcl-x(L) and Mcl-1 in iCCA tissues. Cell death of CCA cell lines upon treatemnt with specific small molecule inhibitors of Bcl-x(L) (Wehi-539), of Mcl-1 (S63845), and Bcl-2 (ABT-199), either alone, in combination with each other or together with chemotherapeutics was assessed by flow cytometry. Targeting Bcl-x(L) induced cell death and augmented the effect of chemotherapy in CCA cells. Combined inhibition of Bcl-x(L) and Mcl-1 led to a synergistic increase in cell death in CCA cell lines. Correlation between Bcl-2 protein expression and survival was analysed within three independent patient cohorts from cancer centers in Germany comprising 656 CCA cases indicating a prognostic value of Bcl-x(L) in CCA depending on the CCA subtype. Collectively, these observations identify Bcl-x(L) as a key protein in cell death resistance of CCA and may pave the way for clinical application