153 research outputs found
Suzbijanje aktivnosti plijesni Botrytis cinerea s pomoÄu bakterije Bacillus coagulans TQ33, izolirane iz obranog mlijeka u prahu
Bacillus coagulans TQ33 is isolated from the skimmed milk powder and has a broad antifungal activity against pathogens such as Botrytis cinerea, Alternaria solani, Phytophthora drechsleri Tucker, Fusarium oxysporum and Glomerella cingulata. The characteristics of active antifungal substances produced by B. coagulans TQ33 and its antifungal effects against the growth of plant pathogenic fungi has been evaluated. The effect of pH, temperature and protease on the antifungal activity of B. coagulans TQ33 was determined. The results of partial characterization of the antifungal compound indicated that its activity is likely to be due to the production of a proteinaceous substance together with other substances. The greenhouse trials suggest that B. coagulans TQ33 has a great potential for the control of plant pathogenic fungi.U radu je izolirana bakterija Bacillus coagulans TQ33 iz obranog mlijeka u prahu, te je ispitan njezin antifungalni uÄinak na razliÄite patogene plijesni, kao ĆĄto su: Botrytis cinerea, Alternaria solani, Phytophthora drechsleri Tucker, Fusarium oxysporum i Glomerella cingulata. Procijenjena su svojstva aktivne tvari izolirane iz bakterije Bacillus coagulans TQ33 i njezin antifungalni uÄinak na rast fitopatogenih plijesni. Ispitan je utjecaj pH-vrijednosti, temperature i prisutnosti razliÄitih proteaza na antifungalnu aktivnost bakterije Bacillus coagulans TQ33. DjelomiÄnom je karakterizacijom antifungalnih spojeva utvrÄeno da je aktivnost bakterije posljedica prisutnosti proteinskih i nekih drugih spojeva. Pokusi su u stakleniku potvrdili da se Bacillus coagulans TQ33 moĆŸe upotrijebiti za kontrolu fitopatogenih plijesni
Proizvodnja novog probiotiÄkog sira tipa Cheddar, veÄe ACE inhibicijske aktivnosti i veÄeg udjela Îł-aminomaslaÄne kiseline, s pomoÄu Lactobacillus casei Zhang, izolirane iz fermentiranoga mlijeÄnog napitka
Cheddar cheese has been manufactured with Lactobacillus casei Zhang as the dairy starter adjunct. L. casei Zhang had previously been isolated from koumiss collected from Xilin Guole in Inner Mongolia and characterized in detail with regard to their probiotic potential. The addition of L. casei Zhang to Cheddar cheese had no adverse effects on sensory criteria. The cheese made with 0.1, 1 and 2 % of the probiotic strain L. casei Zhang adjuncts contained high levels of the Lactobacillus after 6 months of ripening with final counts of 9.6·10^7, 7.7·10^7 and 1.02·10^8 CFU/g, respectively. In the ripe control cheese, without the addition of probiotic strain L. casei Zhang, the number of Lactobacillus reached 5.7·107 CFU/g. Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) analysis was used to distinguish the added L. casei Zhang from the natural flora of the cheese and to determine whether L. casei Zhang grew in the cheese. ACE-inhibitory activity and Îł-aminobutyric acid (GABA) concentrations in the cheese were measured. Compared with control cheese, experimental cheese with 0.1, 1 and 2 % of probiotic strain L. casei Zhang revealed some increase in ACE-inhibitory activity and GABA mass fraction. In the present study, the production of both ACE-inhibitory activity and GABA in the probiotic cheese with the L. casei Zhang adjunct isolated from koumiss has been found for the first time. The results suggest that cheese with the probiotic strain L. casei Zhang showed good potential for application in the management of hypertension.Proizveden je sir tipa Cheddar s pomoÄu dodane kulture Lactobacillus casei Zhang, prethodno izolirane iz fermentiranoga mlijeÄnog napitka âkoumissâ (Xilin Guole, srediĆĄnja Mongolija, Kina). Iscrpno su ispitana probiotiÄka svojstva izolirane kulture. UtvrÄeno je da dodatak kulture nije bitno promijenio senzoriÄka svojstva sira. Sir pripremljen s 0,1 % probiotiÄke kulture imao je nakon 6 mjeseci zrenja 9,6·107 CFU/g, sir s 1 % probiotiÄke kulture 7,7·107 CFU/g, a sir s 2 % probiotiÄke kulture 1,02·108 CFU/g bakterija roda Lactobacillus. U zrelom je kontrolnom uzorku sira (bez dodatka L. casei Zhang) broj bakterija Lactobacillus bio 5,7·107 CFU/g. Provedbom ERIC-PCR analize razluÄena je dodana kultura L. casei Zhang od prirodne mikroflore sira, te utvrÄen njezin rast. TakoÄer je izmjerena veÄa ACE inhibicijska aktivnost te veÄi udio Îł-aminomaslaÄne kiseline u dobivenom siru, u usporedbi s kontrolnim uzorkom. Rezultati pokazuju da se dodatkom probiotiÄke kulture L. casei Zhang dobiva sir boljih svojstava, Äija veÄa primjena pridonosi lijeÄenju poviĆĄenog arterijskog tlaka
Effectiveness of influenza vaccination in patients with end-stage renal disease receiving hemodialysis: a population-based study.
BackgroundLittle is known on the effectiveness of influenza vaccine in ESRD patients. This study compared the incidence of hospitalization, morbidity, and mortality in end-stage renal disease (ESRD) patients undergoing hemodialysis (HD) between cohorts with and without influenza vaccination.MethodsWe used the insurance claims data from 1998 to 2009 in Taiwan to determine the incidence of these events within one year after influenza vaccination in the vaccine (N = 831) and the non-vaccine (N = 3187) cohorts. The vaccine cohort to the non-vaccine cohort incidence rate ratio and hazard ratio (HR) of morbidities and mortality were measured.ResultsThe age-specific analysis showed that the elderly in the vaccine cohort had lower hospitalization rate (100.8 vs. 133.9 per 100 person-years), contributing to an overall HR of 0.81 (95% confidence interval (CI) 0.72-0.90). The vaccine cohort also had an adjusted HR of 0.85 [95% CI 0.75-0.96] for heart disease. The corresponding incidence of pneumonia and influenza was 22.4 versus 17.2 per 100 person-years, but with an adjusted HR of 0.80 (95% CI 0.64-1.02). The vaccine cohort had lowered risks than the non-vaccine cohort for intensive care unit (ICU) admission (adjusted HR 0.20, 95% CI 0.12-0.33) and mortality (adjusted HR 0.50, 95% CI 0.41-0.60). The time-dependent Cox model revealed an overall adjusted HR for mortality of 0.30 (95% CI 0.26-0.35) after counting vaccination for multi-years.ConclusionsESRD patients with HD receiving the influenza vaccination could have reduced risks of pneumonia/influenza and other morbidities, ICU stay, hospitalization and death, particularly for the elderly
Overexpression of the Tomato Pollen Receptor Kinase LePRK1 Rewires Pollen Tube Growth to a Blebbing Mode
The tubular growth of a pollen tube cell is crucial for the sexual reproduction of flowering plants. LePRK1 is a pollen-specific and plasma membraneâlocalized receptor-like kinase from tomato (Solanum lycopersicum). LePRK1 interacts with another receptor, LePRK2, and with KINASE PARTNER PROTEIN (KPP), a Rop guanine nucleotide exchange factor. Here, we show that pollen tubes overexpressing LePRK1 or a truncated LePRK1 lacking its extracellular domain (LePRK1ÎECD) have enlarged tips but also extend their leading edges by producing âblebs.â Coexpression of LePRK1 and tomato PLIM2a, an actin bundling protein that interacts with KPP in a Ca2+-responsive manner, suppressed these LePRK1 overexpression phenotypes, whereas pollen tubes coexpressing KPP, LePRK1, and PLIM2a resumed the blebbing growth mode. We conclude that overexpression of LePRK1 or LePRK1ÎECD rewires pollen tube growth to a blebbing mode, through KPP- and PLIM2a-mediated bundling of actin filaments from tip plasma membranes. Arabidopsis thaliana pollen tubes expressing LePRK1ÎECD also grew by blebbing. Our results exposed a hidden capability of the pollen tube cell: upon overexpression of a single membrane-localized molecule, LePRK1 or LePRK1ÎECD, it can switch to an alternative mechanism for extension of the leading edge that is analogous to the blebbing growth mode reported for Dictyostelium and for Drosophila melanogaster stem cells.Fil: Gui, Cai Ping. Chinese Academy of Sciences; RepĂșblica de ChinaFil: Dong, Xin. Chinese Academy of Sciences; RepĂșblica de ChinaFil: Liu, Hai Kuan. Chinese Academy of Sciences; RepĂșblica de ChinaFil: Huang, Wei Jie. Chinese Academy of Sciences; RepĂșblica de ChinaFil: Zhang, Dong. Chinese Academy of Sciences; RepĂșblica de ChinaFil: Wang, Shu Jie. Chinese Academy of Sciences; RepĂșblica de ChinaFil: Barberini, MarĂa Laura. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de Investigaciones en IngenierĂa GenĂ©tica y BiologĂa Molecular "Dr. HĂ©ctor N. Torres"; ArgentinaFil: Gao, Xiao Yan. Chinese Academy of Sciences; RepĂșblica de ChinaFil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de Investigaciones en IngenierĂa GenĂ©tica y BiologĂa Molecular "Dr. HĂ©ctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y BiologĂa Experimental; ArgentinaFil: McCormick, Sheila. University of California at Berkeley; Estados UnidosFil: Tang, Wei Hua. Chinese Academy of Sciences; RepĂșblica de China. University of California at Berkeley; Estados Unido
A dual pore carbon aerogel based air cathode for a highly rechargeable lithium-air battery
International audienceCathode structure plays a vital role in lithium-air battery for that it can provide space for discharged products accommodation and free path for oxygen, eâ and Li+ transport. However, pore blockage, cathode passivation and degradation all result in low discharge rates and poor cycling capability. To get rid of these predicaments, a novel highly conductive dual pore carbon aerogel based air cathode is fabricated to construct a lithium-air battery, which exhibits 18 to 525 cycles in the LiTFSI/sulfolane electrolyte at a current density varying from 1.00Â mAÂ cmâ2 to 0.05Â mAÂ cmâ2, accompanied by a high energy efficiency of 78.32%. We postulate that the essence lies in that the as-prepared air cathode inventively create a suitable tri-phase boundary reaction zone, facilitating oxygen and Li+ diffusion in two independant pore channels, thus realizing a relative higher discharge rate capability, lower pore blockage and cathode passivation. Further, pore structure, carbon loading, rate capability, discharge depth and the air's effect are exploited and coordinated, targeting for a high power and reversible lithium-air battery. Such nano-porous carbon aerogel air cathode of novel dual pore structure and material design is expected to be an attractive alternative for lithium-air batteries and other lithium based batteries
Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2
To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of CâA at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the âChinese Pharmacopoeiaâ. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis
Incidence Rate and Predictors of Globus Pallidus Necrosis after Charcoal Burning Suicide
Objective: This study examined predictors of globus pallidus necrosis as there was a paucity of literature of globus pallidus necrosis resulted from carbon monoxide poisoning after charcoal burning suicide. Methods: A total of 67 patients who had attempted charcoal burning suicide were recruited and stratified into two subgroups based on either presence (n = 40) or absence (n = 27) of globus pallidus necrosis. Demographic, clinical, laboratory, and radiographic data were obtained for cross-sectional analysis. All patients were followed to investigate the risks for mortality. Results: The patients aged 36.8 ± 11.1 years (67.2%) were male. Patients with globus pallidus necrosis were younger (p = 0.044) and had less hypertension (p = 0.015) than patients without globus pallidus necrosis. Furthermore, patients with globus pallidus necrosis suffered from severer medical complications, i.e., fever (p = 0.008), acute myocardial injury (p = 0.022), acute rhabdomyolysis (p = 0.022), and neuropsychiatric symptoms (p < 0.001) than patients without globus pallidus necrosis. Moreover, patients with globus pallidus necrosis received less hyperbaric oxygen therapy than without necrosis (p = 0.024). Two patients (3.0%) died on arrival. In a multivariable regression model, it was revealed that acute myocardial injury (odds ratio 4.6, confidence interval 1.1-18.9, p = 0.034) and neuropsychiatric symptoms (odds ratio 8.0, confidence interval 2.0-31.4, p = 0.003), decreased blood bicarbonate level (odds ratio 0.8, confidence interval 0.7-1.0, p = 0.032), and younger age (odds ratio 0.9, confidence interval 0.9-1.0, p = 0.038) were significant predictors for globus pallidus necrosis. Conclusion: Although patients who had attempted charcoal burning suicide had a low mortality rate (3.0%), globus pallidus necrosis was not uncommon (59.7%) in this population. Further studies are warranted.Chang Gung Memorial Hospital, Linkou, TaiwanChang Gung Memorial Hospital [CLRPG3D0016, CORPG5G0051]Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
Hungry bone syndrome in peritoneal dialysis patients after parathyroid surgery
Secondary hyperparathyroidism (SHPT) is a common complication of end-stage kidney disease (ESKD). Hungry bone syndrome (HBS) occurs frequently in patients on maintenance dialysis receiving parathyroidectomy for refractory SHPT. However, there is scanty study investigating the clinical risk factors that predict postoperative HBS, and its outcome in peritoneal dialysis (PD) patients. We conducted a single-center retrospective study to analyze 66 PD patients who had undergone parathyroidectomy for secondary hyperparathyroidism at Chang Gung Memorial Hospital between 2009 and 2019. The patients were stratified into two groups based on the presence ( n=47) or absence (n=19) of HBS after parathyroidectomy. Subtotal parathyroidectomy was the most common surgery performed (74.2%), followed by total parathyroidectomy with autoimplantation (25.8%). Pathological examination of all surgical specimens revealed parathyroid hyperplasia (100%). Patients with HBS had lower levels of postoperative nadir corrected calcium, higher alkaline phosphate (ALP), and higher potassium levels compared with patients without HBS (all P<0.05). A multivariate logistic regression model confirmed that lower preoperative serum calcium level (OR 0.354, 95% CI 0.133â0.940, P=0.037), higher ALP (OR 1.026, 95% CI 1.008â1.044, P=0.004), and higher potassium level (OR 6.894, 95% CI 1.806â26.317, P=0.005) were associated with HBS after parathyroidectomy. Patients were followed for 58.2±30.8 months after the surgery. There was no significant difference between HBS and non-HBS groups in persistence (P=0.496) or recurrence (P=1.000) of hyperparathyroidism. The overall mortality rate was 10.6% with no significant difference found between both groups (P=0.099). We concluded that HBS is a common complication (71.2%) of parathyroidectomy for SHPT and should be managed appropriately
The 2nd Workshop on Maritime Computer Vision (MaCVi) 2024
The 2nd Workshop on Maritime Computer Vision (MaCVi) 2024 addresses maritime
computer vision for Unmanned Aerial Vehicles (UAV) and Unmanned Surface
Vehicles (USV). Three challenges categories are considered: (i) UAV-based
Maritime Object Tracking with Re-identification, (ii) USV-based Maritime
Obstacle Segmentation and Detection, (iii) USV-based Maritime Boat Tracking.
The USV-based Maritime Obstacle Segmentation and Detection features three
sub-challenges, including a new embedded challenge addressing efficicent
inference on real-world embedded devices. This report offers a comprehensive
overview of the findings from the challenges. We provide both statistical and
qualitative analyses, evaluating trends from over 195 submissions. All
datasets, evaluation code, and the leaderboard are available to the public at
https://macvi.org/workshop/macvi24.Comment: Part of 2nd Workshop on Maritime Computer Vision (MaCVi) 2024 IEEE
Xplore submission as part of WACV 202
Genome-Wide Association Study of Treatment Refractory Schizophrenia in Han Chinese
We report the first genome-wide association study of a joint analysis using 795 Han Chinese individuals with treatment-refractory schizophrenia (TRS) and 806 controls. Three loci showed suggestive significant association with TRS were identified. These loci include: rs10218843 (Pâ=â3.04Ă10â7) and rs11265461 (Pâ=â1.94Ă10â7) are adjacent to signaling lymphocytic activation molecule family member 1 (SLAMF1); rs4699030 (Pâ=â1.94Ă10â6) and rs230529 (Pâ=â1.74Ă10â7) are located in the gene nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1); and rs13049286 (Pâ=â3.05Ă10â5) and rs3827219 (Pâ=â1.66Ă10â5) fall in receptor-interacting serine/threonine-protein kinase 4 (RIPK4). One isolated single nucleotide polymorphism (SNP), rs739617 (Pâ=â3.87Ă10â5) was also identified to be associated with TRS. The -94delATTG allele (rs28362691) located in the promoter region of NFKB1 was identified by resequencing and was found to associate with TRS (Pâ=â4.85Ă10â6). The promoter assay demonstrated that the -94delATTG allele had a significant lower promoter activity than the -94insATTG allele in the SH-SY5Y cells. This study suggests that rs28362691 in NFKB1 might be involved in the development of TRS
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