Efeitos cr?nicos da ivabradina sobre o controle auton?mico t?nico cardiovascular e o remodelamento ventricular em ratos submetidos ? desnutri??o proteica p?s-desmame.

Programa de P?s-Gradua??o em Ci?ncias Biol?gicas. N?cleo de Pesquisas em Ci?ncias Biol?gicas, Pr?-Reitoria de Pesquisa de P?s Gradua??o, Universidade Federal de Ouro Preto.A desnutri??o ? uma s?ndrome cl?nica de car?ter social, de natureza multifatorial, caracterizada pela exist?ncia de um desequil?brio nutricional que dificulta o funcionamento adequado do organismo. A desnutri??o imposta em fases importantes do desenvolvimento apresenta efeitos severos e permanentes, proporcionando um comprometimento do controle cardiovascular, acometendo o sistema nervoso aut?nomo (SNA). Adicionalmente, o processo patol?gico da desnutri??o pode estar associado a diversas altera??es fisiol?gicas, ocasionando um preju?zo na performance mioc?rdica, acarretando em remodelamento ventricular e disfun??o card?aca. Estudos em ratos demostraram eleva??o da corrente funny (If) em cora??es hipertrofiados ou com insufici?ncia card?aca (IC). A If, corrente cati?nica dos canais ativados por hiperpolariza??o e modulados por nucleot?deos c?clicos (HCNs), ? essencial para a despolariza??o espont?nea da membrana das c?lulas e em situa??es onde h? remodelamento card?aco, o aumento da densidade da If, pode ser um substrato para a arritmog?nese e taquicardia na desnutri??o proteica. Sabendo que a ivabradina, f?rmaco que bloqueia os canais HCNs, ? capaz de reduzir a frequ?ncia card?aca (FC) por meio da diminui??o da If e contribuir para melhora do remodelamento card?aco em cora??es hipertrofiados e com IC, torna-se relevante o estudo da a??o deste f?rmaco sobre os par?metros card?acos na desnutri??o proteica. Desse modo, objetivamos avaliar o efeito da ivabradina sobre o controle auton?mico t?nico da frequ?ncia card?aca e remodelamento ventricular esquerdo de ratos submetidos a desnutri??o proteica p?s-desmame. Para tal, realizou-se o protocolo nutricional (dieta padr?o e dieta hipoproteica) por um per?odo de 35 dias, seguida do tratamento cr?nico com ivabradina (1mg/Kg/dia, i.p.) ou PBS (1mL/Kg, i.p.) por 8 dias consecutivos. Logo ap?s, avaliamos o efeito da ivabradina sobre a FC de repouso e FC intr?nseca (FCi), controle auton?mico card?aco e peso relativo do ventr?culo esquerdo. Desse modo, nossos resultados apontam que a ivabradina diminuiu a FC de repouso e interessantemente, reverteu as altera??es do SNA, diminuindo a atividade simp?tica card?aca e aumentando a atividade parassimp?tica card?aca que estavam alteradas na desnutri??o, bem como, melhorou o ?ndice simpato-vagal e proporcionou a redu??o da FCi nesses animais. Observamos tamb?m que a ivabradina melhorou o peso relativo do ventr?culo esquerdo, um marcador de remodelamento card?aco. Portanto, nossos resultados sugerem que a ivabradina apresenta importante papel em situa??es patol?gicas (i.e. desnutri??o) revertendo as altera??es do controle auton?mico do cora??o e melhorando o remodelamento card?aco.Malnutrition is a social clinical syndrome, of a multifactorial nature, characterized by the existence of a nutritional imbalance, difficulting proper functioning of the body. Malnutrition, when imposed at important stages of development, cause severe and permanent damages, leading to an impairment of cardiovascular control, affecting the autonomic nervous system (ANS). In addition, the pathological process of malnutrition may be associated with several physiological adjustments, causing myocardial performance impairment, leading to ventricular remodeling and cardiac dysfunction. Studies in rats, showed that animals with hypertrophied hearts or heart failure, present an elevation of the funny current (If). The If, a cationic current derived from the hyperpolarization-activated cyclic nucleotides gated (HCNs) channels, is essential for cell membrane spontaneous depolarization. In cardiac remodeling, the elevation of the If, may be a substrate for arrhythmogenesis and tachycardia in protein malnutrition. Knowing that ivabradine, a drug that blocks the HCNs channels, is capable of reducing heart rate (HR) and to improve cardiac remodeling in hypertrophied hearts and in heart failure, studying the ivabradine action on cardiac adjustments caused by protein malnutrition, becomes relevant. Thus, we aim to evaluate the effect of ivabradine on the autonomic control of heart rate and left ventricular remodeling of rats submitted to post-weaning protein malnutrition. For that, we submitted rats to a nutritional protocol (standard diet and hypoprotein diet) for a period of 35 days, followed by ip. treatment with ivabradine (1mg / kg / day) or PBS (1mL / kg) for 8 consecutive days. Right after, we evaluated the effect of ivabradine on resting HR and intrinsic HR (iHR), cardiac autonomic control and relative weight of the left ventricle. Thereby, our results indicate that ivabradine decreases resting HR and, interestingly, reverted the changes in the ANS, decreasing cardiac sympathetic activity and increasing cardiac parasympathetic activity that were altered in malnutrition, as well as improving the sympatho-vagal index and providing a reduction of the iHR in these animals. We also observed that ivabradine improved the relative weight of the left ventricle, a marker of cardiac remodeling. In summary, our results suggest that ivabradine plays an important role in pathological conditions (i.e. malnutrition), reverting the cardiac autonomic control and improving cardiac remodeling

Salmonella clinical isolates from Brazilian pig herds: genetic relationship and antibiotic resistance profiling

In Brazil, since 2011 clinical cases of salmonellosis has been increasing substantially. Nevertheless, few information is available about the antimicrobial profile, distribution, serotypes and genetic relationship among the strains. The objectives of this study were: to identify the Salmonella serotypes, to characterize the in vitro antimicrobial resistance profiles and to determine the genetic relationship of clinical isolates in Brazil. During 2016, clinical isolates of Salmonella (111) from nine States were sent to Embrapa Swine and Poultry for complementary analysis. First, isolates were serotyped by Kauffmann White Scheme. In parallel, the strains were tested against fifteen antimicrobials by disk diffusion method and genotyping was performed by Pulsed Field Gel Electrophoresis (PFGE) using the XbaI restriction enzyme. As expected, the main serovars found were Typhimurium and Choleraesuis. Four strains showed resistance to only one antimicrobial and 76.5% (85/111) were considered multi-resistant. The highest level of resistance was found against to tetracycline. More than 80% of the strains were susceptible to fosfomycin, lincomycin/spectinomycin and norfloxacin. It was possible to identify one major Choleraesuis clonal group present in different Brazilian States. Further, several small clonal groups were obtained for Typhimurium. In conclusion, clinical salmonellosis caused by Typhimurium and Choleraesuis is endemic in pig production areas and the majority of the strains are multi-resistant

Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children.

Background: The evidence that cardiovascular disease begins in childhood and adolescence, especially in the presence of excess weight, is associated with dysfunction on adipokine pro-inflammatory secretion. These affect glucose metabolism and lead to other complications related to insulin resistance and cardiovascular disease. This study assessed the association of anthropometric and metabolic parameters related to obesity, cardiovascular risk, and insulin resistance with concentrations of resistin and visfatin, in children. Methods: A cross-sectional study was developed with 178 children of 6?10 years old enrolled in public city schools. Anthropometric data, composition body, clinical, and biochemical were measured according to standard procedures. We used multiple regression models by stepwise method to evaluate the associations of resistin and visfatin with variables of interest. Results: In healthy weight children, resistin was associated with LDL cholesterol, visfatin, atherogenic index, and waist-to-height ratio, whereas in obese children resistin was associated with visfatin and interaction between conicity index and HOMA-AD. Furthermore, in healthy weight children, visfatin was associated to resistin and triceps skinfold thickness and negatively associated to HOMA-AD, while in obese ones visfatin was associated with waistto- height ratio, atherogenic index, resistin, and interaction between trunk adiposity index and adiponectin and was negatively associated with the HOMA-IR index. Conclusions: Our study shows an association between anthropometric and biochemical variables related to visceral fat and inflammation. These results suggest the resistin and visfatin as good pro-inflammatory markers. In addition, both adipokines are strongly related to central obesity, in children

TRANSTORNOS DO SONO EM IDOSOS E SUA RELAÇÃO COM CONDIÇÕES PSIQUIÁTRICAS: UMA REVISÃO BIBLIOGRÁFICA

Introduction: Aging is associated with physiological and psychological changes that can impact sleep patterns, such as reduced sleep efficiency, fragmentation and a higher incidence of disorders such as insomnia and sleep apnea syndrome. Objective: To analyze the relationship between sleep disorders in the elderly and their implications for psychiatric conditions. Methodology: This is a bibliographical review, using articles present in the following databases: PubMed, Web of Science and Scopus. Original articles and systematic reviews in English and Portuguese published between 2010 and 2024 were included, which addressed sleep disorders in elderly people and their implications for psychiatric conditions. After analysis, 23 articles were selected to prepare this bibliographic review. Results and Discussion: Older adults are more likely to experience a variety of sleep disorders due to age-related physiological changes, chronic medical conditions, and psychosocial factors. Furthermore, many common psychiatric conditions, such as depression, anxiety, and dementia, have a close association with sleep disorders in the elderly. Conclusion: When facing the challenges of sleep disorders in the elderly, it is essential to adopt a patient-centered approach that prioritizes the elderly's well-being and quality of life.Introducción: El envejecimiento se asocia con cambios fisiológicos y psicológicos que pueden impactar los patrones de sueño, como reducción de la eficiencia del sueño, fragmentación y mayor incidencia de trastornos como el insomnio y el síndrome de apnea del sueño. Objetivo: Analizar la relación entre los trastornos del sueño en adultos mayores y sus implicaciones para las condiciones psiquiátricas. Metodología: Se trata de una revisión bibliográfica, utilizando artículos presentes en las siguientes bases de datos: PubMed, Web of Science y Scopus, se incluyeron artículos originales y revisiones sistemáticas en inglés y portugués publicados entre 2010 y 2024, que abordaron los trastornos del sueño en personas mayores y sus Implicaciones para las condiciones psiquiátricas. Luego del análisis, se seleccionaron 23 artículos para elaborar esta revisión bibliográfica. Resultados y discusión: Los adultos mayores tienen más probabilidades de experimentar una variedad de trastornos del sueño debido a cambios fisiológicos relacionados con la edad, afecciones médicas crónicas y factores psicosociales. Además, muchas afecciones psiquiátricas comunes, como la depresión, la ansiedad y la demencia, tienen una estrecha asociación con los trastornos del sueño en las personas mayores. Conclusión: Al enfrentar los desafíos de los trastornos del sueño en las personas mayores, es fundamental adoptar un enfoque centrado en el paciente que priorice el bienestar y la calidad de vida de las personas mayores.O envelhecimento está associado a alterações fisiológicas e psicológicas que podem impactar o padrão de sono, como a redução da eficiência do sono, a fragmentação e a maior incidência de distúrbios como a insônia e a síndrome da apneia do sono.Objetivo: Analisar a relação entre os distúrbios do sono em idosos e suas implicações nas condições psiquiátricas. Metodologia:Trata-se de uma revisão bibliográfica, utilizando artigos presentes nas seguintes bases de dados: PubMed, Web of Science e Scopus Foram incluídos artigos originais e revisões sistemáticas em inglês e português publicadas entre 2010 e 2024, que abordassem sobredistúrbios do sono em idosos e suas implicações nas condições psiquiátricas. Após a análise, foram selecionados 23 artigos para a confecção dessa revisão bibliográfica.  Resultados e Discussão: Os idosos são mais propensos a experimentar uma variedade de distúrbios do sono devido a mudanças fisiológicas relacionadas à idade, condições médicas crônicas e fatores psicossociais. Além disso, muitas condições psiquiátricas comuns, como depressão, ansiedade e demência, têm uma associação estreita com distúrbios do sono em idosos. Conclusão: Ao enfrentar os desafios dos transtornos do sono em idosos, é essencial adotar uma abordagem centrada no paciente, que priorize o bem-estar e a qualidade de vida do idoso.Introdução: O envelhecimento está associado a alterações fisiológicas e psicológicas que podem impactar o padrão de sono, como a redução da eficiência do sono, a fragmentação e a maior incidência de distúrbios como a insônia e a síndrome da apneia do sono.Objetivo: Analisar a relação entre os distúrbios do sono em idosos e suas implicações nas condições psiquiátricas. Metodologia:Trata-se de uma revisão bibliográfica, utilizando artigos presentes nas seguintes bases de dados: PubMed, Web of Science e Scopus Foram incluídos artigos originais e revisões sistemáticas em inglês e português publicadas entre 2010 e 2024, que abordassem sobredistúrbios do sono em idosos e suas implicações nas condições psiquiátricas. Após a análise, foram selecionados 23 artigos para a confecção dessa revisão bibliográfica.  Resultados e Discussão: Os idosos são mais propensos a experimentar uma variedade de distúrbios do sono devido a mudanças fisiológicas relacionadas à idade, condições médicas crônicas e fatores psicossociais. Além disso, muitas condições psiquiátricas comuns, como depressão, ansiedade e demência, têm uma associação estreita com distúrbios do sono em idosos. Conclusão: Ao enfrentar os desafios dos transtornos do sono em idosos, é essencial adotar uma abordagem centrada no paciente, que priorize o bem-estar e a qualidade de vida do idoso

Tobacco-free cigarette smoke exposure induces anxiety and panic-related behaviours in male wistar rats.

Smokers, who generally present with lung damage, are more anxious than non-smokers and have an associated augmented risk of panic. Considering that lung damage signals specific neural pathways that are related to affective responses, the aim of the present study was to evaluate the influence of pulmonary injury on anxiety and panic-like behaviours in animals exposed to cigarette smoke with and without tobacco. Male Wistar rats were divided into the following groups: a control group (CG); a regular cigarette group (RC); and a tobacco-free cigarette (TFC) group. Animals were exposed to twelve cigarettes per day for eight consecutive days. The animals were then exposed to an elevated T-maze and an open field. The RC and TFC groups presented increases in inflammatory cell inflow, antioxidant enzyme activity, and TBARS levels, and a decrease in the GSH/GSSG ratio was observed in the TFC group. Exposure to RC smoke reduced anxiety and panic-related behaviours. On the other hand, TFC induced anxiety and panic-related behaviours. Thus, our results contradict the concept that nicotine is solely accountable for shifted behavioural patterns caused by smoking, in that exposure to TFC smoke causes anxiety and panic-related behaviours.Cigarette smoke exposure is associated with anxiety states. Smokers are more anxious than non-smokers1, while cigarette smoking cessation is associated with increased levels of anxiety and stress, as the nicotine in cigarettes has been shown to have anxiolytic effects2. Moreover, smoking is also associated with an augmented risk of panic attacks, and quitting smoking could help reduce this risk3. Importantly, in a study conducted by Amaring and colleagues, it was reported that 72% of panic disorder patients declared that they were regular smokers at the onset of their disease4. Cigarette smoke is also one of the several agents and environmental factors that can trigger oxidative stress and pulmonary damage5. Cigarette smoke causes cellular recruitment, lipid peroxidation, production of inflammatory mediators, and oxidative stress6?11. For instance, studies in mice have shown that exposure to short-term cigarette smoke evokes an increase in inflammatory cell inflow and oxidative damage6,9. In general, exposure to pollutants induces pulmonary inflammation through the generation of oxidative stress12,13, defined as the imbalance in reactive oxygen species production, to the detriment of the antioxidant defence systems14. Importantly, exposure to ambient air particles not only induces pulmonary inflammation but also behavioural disorders both in humans and in mice15. Currently, the majority of anxiety studies associated with cigarette smoking have focused on the anxiolytic effects of nicotine2. However, it has been shown that lung damage can induce central nervous system responses by activating specific neuronal pathways16,17, which include those linked to affective responses, such as anxiety and panic18. This raises the question of whether the anxiety and panic-type behaviour associated with smoking might be related not only to the nicotine or to tobacco?s other constituents but also to lung damage

Promoção de cuidados à saúde: risco cardiovascular em Nova Friburgo

Chronic non-communicable diseases (NCDs) are multifactorial long-term diseases that develop along life. They share modifiable risk factors, such as smoking, alcohol consumption, physical inactivity, diet and obesity. Since these risk factors are preventable, it is extremely important to carry out actions in order to educate the population about the pathophysiology of NCDs and its risk factors. The main goals of the Program of Health Care Promotion are to monitor cardiovascular risk factors in the diabetic and hypertensive population as well as healthy individuals from Nova Friburgo, and to provide information regarding modifiable risk factors. Thus, this article aims to report the Program's experience during its first year, with regard to its implementation, impact of the activities developed on the population and population feedback, lessons learned, challenges and future directions. In the first year of implementation, we observed that the Program had positively impacted the population, by monitoring their health status periodically and by means of health education.Las enfermedades crónicas no transmisibles (ENT) son enfermedades multifactoriales que se desarrollan a lo largo de la vida y son de  duración prolongada. Comparten entre si factores de riesgo modificables, como el tabaquismo, el alcohol, la inactividad física, la dieta y la obesidad.  Teniendo en cuenta que estos factores se pueden prevenir, es muy importante conducir acciones dirigidas a educar a la población acerca de la fisiopatología de las enfermedades no transmisibles y sus factores de riesgo. El Programa Promoción de la Salud tiene como objetivos principales controlar los factores de riesgo cardiovascular en individuos diabéticos e hipertensos, así como los individuos sanos de Nova Friburgo y educar a la población acerca de los factores de riesgo modificables. Por lo tanto, este artículo tiene por objeto informar de la experiencia del Programa durante su primer año de ejecución, con respecto a su aplicación, el impacto de las actividades sobre la población y su opinión, las lecciones aprendidas, desafíos y perspectivas futuras. En el primer año, se observó que el programa ha tenido un impacto positivo en la población atendida, proporcionando un seguimiento de su salud o condición a través de evaluaciones periódicas, así como la educación para la salud.As doenças crônicas não transmissíveis (DCNT) são doenças multifatoriais que se desenvolvem no decorrer da vida e são de longa duração. Elas compartilham entre si fatores de risco modificáveis, entre eles o tabagismo, o álcool, a inatividade física, a alimentação e a obesidade. Sabendo-se que estes fatores são passíveis de prevenção, é de extrema importância a realização de ações que visem a educação da população acerca da fisiopatologia das DCNT e seus fatores de risco. O Programa Promoção de Cuidados à Saúde tem como objetivos principais monitorar os fatores de risco cardiovascular de indivíduos diabéticos e hipertensos, assim como indivíduos saudáveis, da população de Nova Friburgo e educar a população acerca dos fatores de risco modificáveis. Sendo assim, este artigo tem como objetivo relatar a experiência do Programa durante o seu primeiro ano de execução, ressaltando o impacto das atividades sobre a população e devolutivas, aprendizados, dificuldades e perspectivas futuras. Neste primeiro ano foi possível observar que o programa impactou positivamente a população atendida, proporcionando a estes um acompanhamento de sua saúde ou patologia, por meio de avaliações periódicas, assim como pela educação em saúde

Compartimentos de carbono orgânico em Latossolo cultivado com hortaliças sob diferentes manejos

Resumo: O objetivo deste trabalho foi avaliar os teores de carbono orgânico total (COT) e das frações granulométricas da matéria orgânica de um Latossolo Vermelho cultivado com hortaliças, sob diferentes sistemas de manejo e de cultivo de plantas de cobertura. O experimento foi realizado por seis anos, em delineamento de blocos ao caso, em arranjo fatorial 3x2, com três sistemas de manejo do solo - plantio direto (SPD), preparo reduzido (PPR) e preparo convencional (SPC) - e dois cultivos de cobertura, um de milho solteiro e outro de milho consorciado com mucuna-cinza (Stizolobium niveum) (MM), com quatro repetições. Os teores de COT, estimados para a camada 0,00-0,30 m, foram maiores em SPD e PPR. Independentemente da profundidade avaliada, o consórcio MM promoveu os maiores teores de carbono orgânico particulado (COP), que foram fortemente influenciados pela incorporação da palhada. Em todas as camadas, observaram-se correlações negativas entre os teores de COP e os de carbono orgânico associado a minerais (COAM), indício de ocorrência do efeito "priming". Os teores de COT correlacionaram-se positivamente aos de COAM, o que destaca a importância de mecanismos de estabilização da matéria orgânica no incremento dos teores de COT no solo

MAMMALS IN PORTUGAL : A data set of terrestrial, volant, and marine mammal occurrences in P ortugal

Mammals are threatened worldwide, with 26% of all species being includedin the IUCN threatened categories. This overall pattern is primarily associatedwith habitat loss or degradation, and human persecution for terrestrial mam-mals, and pollution, open net fishing, climate change, and prey depletion formarine mammals. Mammals play a key role in maintaining ecosystems func-tionality and resilience, and therefore information on their distribution is cru-cial to delineate and support conservation actions. MAMMALS INPORTUGAL is a publicly available data set compiling unpublishedgeoreferenced occurrence records of 92 terrestrial, volant, and marine mam-mals in mainland Portugal and archipelagos of the Azores and Madeira thatincludes 105,026 data entries between 1873 and 2021 (72% of the data occur-ring in 2000 and 2021). The methods used to collect the data were: live obser-vations/captures (43%), sign surveys (35%), camera trapping (16%),bioacoustics surveys (4%) and radiotracking, and inquiries that represent lessthan 1% of the records. The data set includes 13 types of records: (1) burrowsjsoil moundsjtunnel, (2) capture, (3) colony, (4) dead animaljhairjskullsjjaws, (5) genetic confirmation, (6) inquiries, (7) observation of live animal (8),observation in shelters, (9) photo trappingjvideo, (10) predators dietjpelletsjpine cones/nuts, (11) scatjtrackjditch, (12) telemetry and (13) vocalizationjecholocation. The spatial uncertainty of most records ranges between 0 and100 m (76%). Rodentia (n=31,573) has the highest number of records followedby Chiroptera (n=18,857), Carnivora (n=18,594), Lagomorpha (n=17,496),Cetartiodactyla (n=11,568) and Eulipotyphla (n=7008). The data setincludes records of species classified by the IUCN as threatened(e.g.,Oryctolagus cuniculus[n=12,159],Monachus monachus[n=1,512],andLynx pardinus[n=197]). We believe that this data set may stimulate thepublication of other European countries data sets that would certainly contrib-ute to ecology and conservation-related research, and therefore assisting onthe development of more accurate and tailored conservation managementstrategies for each species. There are no copyright restrictions; please cite thisdata paper when the data are used in publications.info:eu-repo/semantics/publishedVersio

Geographic patterns of tree dispersal modes in Amazonia and their ecological correlates

Aim: To investigate the geographic patterns and ecological correlates in the geographic distribution of the most common tree dispersal modes in Amazonia (endozoochory, synzoochory, anemochory and hydrochory). We examined if the proportional abundance of these dispersal modes could be explained by the availability of dispersal agents (disperser-availability hypothesis) and/or the availability of resources for constructing zoochorous fruits (resource-availability hypothesis). Time period: Tree-inventory plots established between 1934 and 2019. Major taxa studied: Trees with a diameter at breast height (DBH) ≥ 9.55 cm. Location: Amazonia, here defined as the lowland rain forests of the Amazon River basin and the Guiana Shield. Methods: We assigned dispersal modes to a total of 5433 species and morphospecies within 1877 tree-inventory plots across terra-firme, seasonally flooded, and permanently flooded forests. We investigated geographic patterns in the proportional abundance of dispersal modes. We performed an abundance-weighted mean pairwise distance (MPD) test and fit generalized linear models (GLMs) to explain the geographic distribution of dispersal modes. Results: Anemochory was significantly, positively associated with mean annual wind speed, and hydrochory was significantly higher in flooded forests. Dispersal modes did not consistently show significant associations with the availability of resources for constructing zoochorous fruits. A lower dissimilarity in dispersal modes, resulting from a higher dominance of endozoochory, occurred in terra-firme forests (excluding podzols) compared to flooded forests. Main conclusions: The disperser-availability hypothesis was well supported for abiotic dispersal modes (anemochory and hydrochory). The availability of resources for constructing zoochorous fruits seems an unlikely explanation for the distribution of dispersal modes in Amazonia. The association between frugivores and the proportional abundance of zoochory requires further research, as tree recruitment not only depends on dispersal vectors but also on conditions that favour or limit seedling recruitment across forest types

Geography and ecology shape the phylogenetic composition of Amazonian tree communities

Aim: Amazonia hosts more tree species from numerous evolutionary lineages, both young and ancient, than any other biogeographic region. Previous studies have shown that tree lineages colonized multiple edaphic environments and dispersed widely across Amazonia, leading to a hypothesis, which we test, that lineages should not be strongly associated with either geographic regions or edaphic forest types. Location: Amazonia. Taxon: Angiosperms (Magnoliids; Monocots; Eudicots). Methods: Data for the abundance of 5082 tree species in 1989 plots were combined with a mega-phylogeny. We applied evolutionary ordination to assess how phylogenetic composition varies across Amazonia. We used variation partitioning and Moran\u27s eigenvector maps (MEM) to test and quantify the separate and joint contributions of spatial and environmental variables to explain the phylogenetic composition of plots. We tested the indicator value of lineages for geographic regions and edaphic forest types and mapped associations onto the phylogeny. Results: In the terra firme and várzea forest types, the phylogenetic composition varies by geographic region, but the igapó and white-sand forest types retain a unique evolutionary signature regardless of region. Overall, we find that soil chemistry, climate and topography explain 24% of the variation in phylogenetic composition, with 79% of that variation being spatially structured (R$^{2}$ = 19% overall for combined spatial/environmental effects). The phylogenetic composition also shows substantial spatial patterns not related to the environmental variables we quantified (R$^{2}$ = 28%). A greater number of lineages were significant indicators of geographic regions than forest types. Main Conclusion: Numerous tree lineages, including some ancient ones (>66 Ma), show strong associations with geographic regions and edaphic forest types of Amazonia. This shows that specialization in specific edaphic environments has played a long-standing role in the evolutionary assembly of Amazonian forests. Furthermore, many lineages, even those that have dispersed across Amazonia, dominate within a specific region, likely because of phylogenetically conserved niches for environmental conditions that are prevalent within regions