Factores protectores y de riesgo del sueño de los niños pequeños y del estrés de los padres

The current study examined the network of relationships among toddlers’ quality of sleep and emotion regulation, parental bedtime practices and involvement, parental self-perceived social support and stress, integrating a novel combination of psychosocial dimensions into a predictive model of quality of sleep and parental stress in a sample of 80 families with 2–3-year-old children. The results indicated that infants’ emotion regulation, as well as proximal context variables, considering both maternal and paternal psychosocial functioning predicted variance in parental levels of stress. Furthermore, the findings showed that the number of child’s night awakenings reported by mothersand thetimerequested bytoddlersto fallasleep, were significantly related to parental distress levels as referred by both parents.En el presente estudio se examinó la red de relaciones entre la calidad del sueño y la regulación de las emociones de los niños pequeños, las prácticas y la participación de los padres a la hora de acostarse, el apoyo social y el estrés de los padres, integrando una novedosa combinación de dimensiones psicosociales en un modelo predictivo de la calidad del sueño y el estrés de los padres en una muestra de 80 familias con niños de 2 a 3 años de edad. Los resultados indicaron que la regulación de las emociones de los lactantes, así como las variables de contexto próximo, teniendo en cuenta el funcionamiento psicosocial tanto materno como paterno, predecían la varianza de los niveles de estrés de los padres. Además, los resultados mostraron que el número de vigilancias nocturnas del niño comunicadas por las madres y el tiempo solicitado por los niños pequeños para dormirse, estaban significativamente relacionados con los niveles de estrés de los padres según lo referido por ambos padres

Sleep Quality, Emotion Regulation and Parenting Stress in Children with Congenital Heart Disease

The aim of this study is to evaluate parental perceptions of parent-infant bedtime interactions and quality of sleep, after hospital discharge, in a group of children diagnosed at birth with congenital heart disease (CHD), as compared with the perceptions of parents in a control group of children who were healthy at birth. More specifically, we evaluated the associations between parental stress, parental perceptions of infant emotion regulation, and infants’ bedtime and sleep routines in each of the two groups. Fifty Italian intact two-parent families (23 boys) of toddlers ageing from 11 to 36 months (M= 23.42, SD=7.10) were recruited. 20 families of CHD children group were recruited from the Department of Cardiology at the Bambino Gesù Children’s Hospital in Rome, Italy; 30 families of the healthy children group were recruited from two childcare units. Parents completed Emotion Regulation Checklist (Shields Cicchetti, 1997), Parent-Child Sleep Interaction Scale (PSIS; Alfano et al., 2013), Parent-Stress Index-Short Form (PSI-SF; Abidin, 1990) and ad-hoc semi-structured interview on child’s sleep quality. The independent-samples t-test evidenced that parents of healthy children reported significantly higher scores on children's emotion regulation compared with the CHD group Specifically CHD children and healthy children's emotion regulation reported both by mothers (respectively CHD children’s mothers: M= 26.11, SD= 2.9; healthy children’s mothers: M= 28.85, SD= 2.71; t(37) = 3.10, p= .004) and fathers (respectively CHD children’s fathers: M=25.76, SD= 2.79; healthy children’s fathers: M= 27.37, SD= 2.02; t(31)= 2.71, p= .010). Main correlational findings showed in the CHD children’s group that parenting stress total scores were positive related to difficult parent-infant bedtime interactions both for mothers (r=.58, p .01) and fathers (r= .70, p .01). Results show significant differences in emotion regulation between the two groups. The results of this research will show to the clinicians the aspects of parent-infant bedtime interactions to be addressed in parents of children with CHD

Imaging-based representation and stratification of intra-tumor heterogeneity via tree-edit distance

Personalized medicine is the future of medical practice. In oncology, tumor heterogeneity assessment represents a pivotal step for effective treatment planning and prognosis prediction. Despite new procedures for DNA sequencing and analysis, non-invasive methods for tumor characterization are needed to impact on daily routine. On purpose, imaging texture analysis is rapidly scaling, holding the promise to surrogate histopathological assessment of tumor lesions. In this work, we propose a tree-based representation strategy for describing intra-tumor heterogeneity of patients affected by metastatic cancer. We leverage radiomics information extracted from PET/CT imaging and we provide an exhaustive and easily readable summary of the disease spreading. We exploit this novel patient representation to perform cancer subtyping according to hierarchical clustering technique. To this purpose, a new heterogeneity-based distance between trees is defined and applied to a case study of prostate cancer. Clusters interpretation is explored in terms of concordance with severity status, tumor burden and biological characteristics. Results are promising, as the proposed method outperforms current literature approaches. Ultimately, the proposed method draws a general analysis framework that would allow to extract knowledge from daily acquired imaging data of patients and provide insights for effective treatment planning

[¹⁸F]FMCH PET/CT biomarkers and similarity analysis to refine the definition of oligometastatic prostate cancer

Background:The role of image-derived biomarkers in recurrent oligometastatic Prostate Cancer (PCa) is unexplored. This paper aimed to evaluate [18F]FMCH PET/CT radiomic analysis in patients with recurrent PCa after primary radical therapy. Specifically, we tested intra-patient lesions similarity in oligometastatic and plurimetastatic PCa, comparing the two most used definitions of oligometastatic disease.Methods:PCa patients eligible for [18F]FMCH PET/CT presenting biochemical failure after first-line curative treat-ments were invited to participate in this prospective observational trial. PET/CT images of 92 patients were visually and quantitatively analyzed. Each patient was classified as oligometastatic or plurimetastatic according to the total number of detected lesions (up to 3 and up to 5 or > 3 and > 5, respectively). Univariate and intra-patient lesions’ similarity analysis were performed.Results: [18F]FMCH PET/CT identified 370 lesions, anatomically classified as regional lymph nodes and distant metastases. Thirty-eight and 54 patients were designed oligometastatic and plurimetastatic, respectively, using a 3-lesion threshold. The number of oligometastic scaled up to 60 patients (thus 32 plurimetastatic patients) with a 5-lesion threshold. Similarity analysis showed high lesions’ heterogeneity. Grouping patients according to the number of metastases, patients with oligometastatic PCa defined with a 5-lesion threshold presented lesions heterogene-ity comparable to plurimetastic patients. Lesions within patients having a limited tumor burden as defined by three lesions were characterized by less heterogeneity.Conclusions:We found a comparable heterogeneity between patients with up to five lesions and plurimetastic patients, while patients with up to three lesions were less heterogeneous than plurimetastatic patients, featuring dif-ferent cells phenotypes in the two groups. Our results supported the use of a 3-lesion threshold to define oligometa-static PCa

A peculiar formula of essential amino acids prevents rosuvastatin myopathy in mice

Aims: Myopathy, characterized by mitochondrial oxidative stress, occurs in ∼10% of statin-treated patients, and a major risk exists with potent statins such as rosuvastatin (Rvs). We sought to determine whether a peculiar branched-chain amino acid-enriched mixture (BCAAem), found to improve mitochondrial function and reduce oxidative stress in muscle of middle-aged mice, was able to prevent Rvs myopathy. Results: Dietary supplementation of BCAAem was able to prevent the structural and functional alterations of muscle induced by Rvs in young mice. Rvs-increased plasma 3-methylhistidine (a marker of muscular protein degradation) was prevented by BCAAem. This was obtained without changes of Rvs ability to reduce cholesterol and triglyceride levels in blood. Rather, BCAAem promotes de novo protein synthesis and reduces proteolysis in cultured myotubes. Morphological alterations of C2C12 cells induced by statin were counteracted by amino acids, as were the Rvs-increased atrogin-1 mRNA and protein levels. Moreover, BCAAem maintained mitochondrial mass and density and citrate synthase activity in skeletal muscle of Rvs-treated mice beside oxygen consumption and ATP levels in C2C12 cells exposed to statin. Notably, BCAAem assisted Rvs to reduce oxidative stress and to increase the anti-reactive oxygen species (ROS) defense system in skeletal muscle. Innovation and Conclusions: The complex interplay between proteostasis and antioxidant properties may underlie the mechanism by which a specific amino acid formula preserves mitochondrial efficiency and muscle health in Rvs-treated mice. Strategies aimed at promoting protein balance and controlling mitochondrial ROS level may be used as therapeutics for the treatment of muscular diseases involving mitochondrial dysfunction, such as statin myopathy

Coinvolgimento lavorativo degli educatori del nido e degli insegnanti della scuola dell’infanzia: studio quali-quantitativo

Research shows as work-engagement, enthusiasm and vigor predict work performance and have positive effects on teacher-student relationships and on students' academic outcomes (Bakker, Schaufeli, Leiter and Taris, 2008; Bakker, Van Emmerik and Euwema, 2006; Duckworth, Quinn and Seligman, 2009). According to our quali-quantitative study, two main aims were set: 1) the evaluation of the predictive role of some dispositional variables (subjective wellbeing, compassion and dispositional positive affect) and of some socio-contextual variables (self/co-regulated proactive strategies, constructive climate) on teachers' work-engagement in educational and scholastic services 0/6 years; 2) the qualitatively exploration of dimensions of promoting well-being in educational services. A group of 189 educators and kindergarten teachers was asked to fill the following questionnaires: the UWES-17 (Schaufeli, 2003), the Santa Clara Brief Compassion Scale (Sprecher and Fehr, 2005), the Proactive Strategy Scale (Salmela-Aro, 2009), the Positive and Negative Affect Schedule (Watson et al, 1988), the Subjective Happiness Scale (Lyuborminsky and Lepper, 1999) and the Teacher self-efficacy and ad hoc interview on components of promoting well-being in educational services. Our main quantitative outcomes showed that compassion and proactive strategies predict higher levels of work-engagement over subjective well-being and dispositional positive affect.La letteratura mostra come il coinvolgimento lavorativo, l’entusiasmo e il vigore dei docenti siano predittivi delle prestazioni lavorative ed abbiano un effetto benefico sulle relazioni positive insegnante-studente e sugli esiti accademici degli studenti (Bakker, Schaufeli, Leiter e Taris, 2008; Bakker, Van Emmerik e Euwema, 2006; Duckworth, Quinn e Seligman, 2009). L’obiettivo del presente studio di carattere quali-quantitativo è stato duplice: 1) valutare il ruolo predittivo,  sul coinvolgimento lavorativo degli operatori dei servizi educativi e scolastici 0/6 anni, di variabili disposizionali (benessere soggettivo, compassione, affettività disposizionale positiva)  e di natura socio-contestuale (strategie proattive auto ed etero regolative, clima costruttivo); 2) esplorare sul piano qualitativo le dimensioni di promozione del benessere nei servizi. Ad un gruppo di 189 educatori e insegnanti della scuola dell’infanzia sono stati somministrati l’UWES-17 (Schaufeli e Bakker, 2004), la Santa Clara Brief Compassion Scale (Hwang, Plante, e Lackey, 2008), la Proactive Strategy Scale (Salmela-Aro, 2009), la Positive and Negative Affect Schedule (Watson, Clark, e Tellegen,  1988), la Subjective Happiness Scale (Lyuborminsky e Lepper, 1999) la Teacher self-efficacy ed un intervista qualitativa costruita ad hoc sugli aspetti di promozione del benessere. I principali esiti quantitativi evidenziano come a parità di benessere soggettivo e di affettività disposizionale positiva, la compassione e le strategie proattive sono predittori di un maggior coinvolgimento lavorativo

A web-delivered group intervention supporting parental sensitivity and self-efficacy: an Italian pilot study

Background: Stable parent-infant relationships and adequate ordinary care significantly support children’s development since the very early stages of life. Principal models of intervention sustain parental skills and foster quality of parent-infant interactions since the early infancy. Standardized programs, with a well-defined focus, of short duration, based on specific methods and techniques, represent an effective tool in supporting parental effort. The present pilot study provides a description and an initial evaluation of the brief online “Con i Genitori” (CiG) Intervention, aimed to enhance parental sensitivity, self-efficacy and reduce stress in parents of typically-developed children aging 0-6 years. Methods: The intervention involved parents of typically-developed children aging 0-6 years. Four interactive group sessions, based on well-known empirically-based programs’ assumptions were delivered. Participants were asked to complete questionnaires at baseline (T0) and after CiG (T1). The assessment included the Tool to measure Parenting Self-Efficacy (TOPSE; Kendall Bloomfield, 2005), Parenting Stress Index-SF for parental distress (PSI; Abidin, 1996), Emotional Regulation Checklist for children’s emotional regulation (ERC; Shields Cicchetti et al., 1997) and Social Provision Scale for social support (SPS; Cutrona and Russell, 1987). A weekly “ad-hoc” questionnaire evaluated parental sensitivity.  Moreover, a semi-structured interview measured participants’ satisfaction and acceptability with the intervention one month after its end. Results: Twelve parents completed all the sessions of the CiG (10 mothers, 2 fathers with mean age = 42.7; SD= 6.3). Children mean age was 3.9 (SD=1.9), 58.3% male. Our results showed statistically significant decrease in parental distress and increased social support after attending CiG. No statistically significant variations were detected considering parental self-efficacy. Conclusions: Our findings confirm the potential value of online-delivered interventions targeting parenthood in infancy, supporting parent-infant relationship and positive parenting from early infancy in a public health community approach. Online delivered programs constitute an important resource for addressing unmet parent mental health needs, which may be particularly widespread following the COVID-19 pandemic, representing a valuable alternative to traditional face-to-face interventions targeting parental wellbeing in infancy

Postharvest Diseases of Pomegranate: Alternative Control Means and a Spiderweb Effect

The pomegranate is a fruit known since ancient times for its beneficial properties. It has recently aroused great interest in the industry and among consumers, leading to a significant increase in demand. Consequently, its cultivation has been boosted all over the world. The pomegranate crop suffers considerable yield losses, especially at the postharvest stage, because it is a “minor crop” with few permitted control means. To control latent (Alternaria spp., Botrytis spp., Coniella spp., Colletotrichum spp., and Cytospora spp.) and wound (Aspergillus spp., Penicillium spp., and Talaromyces spp.) fungal pathogens, different alternative compounds, previously evaluated in vitro, were tested in the field on pomegranate cv. Wonderful. A chitosan solution, a plant protein hydrolysate, and a red seaweed extract were compared with a chemical control treatment, all as preharvest (field application) and postharvest treatments and their combinations. At the end of the storage period, the incidence of stamen infections and external and internal rots, and the severity of internal decay were evaluated. Obtained data revealed that pre- and postharvest application of all substances reduced the epiphytic population on stamens. Preharvest applications of seaweed extract and plant hydrolysate were the most effective treatments to reduce the severity of internal pomegranate decays. Furthermore, the influence of spider (Cheiracanthium mildei) cocoons on the fruit calyx as a possible barrier against postharvest fungal pathogens was assessed in a ‘Mollar de Elche’ pomegranate organic orchard. Compared to no-cocoon fruit (control), the incidence of infected stamens and internal molds in those with spiderwebs was reduced by about 30%, and the mean severity of internal rots was halved. Spiderwebs analyzed via Scanning Electron Microscopy (SEM) disclosed a layered, unordered structure that did not allow for the passage of fungal spores due to its mean mesh size (1 to 20 µm ca). The aims of this research were (I) to evaluate alternative compounds useful to control postharvest pomegranate decays and (II) to evaluate the effectiveness of spiders in reducing postharvest fungal infections by analyzing related mechanisms of action. Alternative control means proposed in the present work and calyx spider colonization may be helpful to reduce postharvest pomegranate diseases, yield losses, and waste production in an integrated control strategy, satisfying organic agriculture and the planned goals of Zero Hunger Challenge launched by the United Nations

Amniotic membrane-mesenchymal stromal cells secreted factors and extracellular vesicle-miRNAs: Anti-inflammatory and regenerative features for musculoskeletal tissues

Human amniotic membrane-derived mesenchymal stromal cells (hAMSCs) are easily obtained in large quantities and free from ethical concerns. Promising therapeutic results for both hAMSCs and their secreted factors (secretome) were described by several invitro and preclinical studies, often for treatment of orthopedic disorders such as osteoarthritis (OA) and tendinopathy. For clinical translation of the hAMSC secretome as cell-free therapy, a detailed characterization of hAMSC-secreted factors is mandatory. Herein, we tested the presence of 200 secreted factors and 754 miRNAs in extracellular vesicles (EVs). Thirty-seven cytokines/chemokines were identified at varying abundance, some of which involved in both chemotaxis and homeostasis of inflammatory cells and in positive remodeling of extracellular matrix, often damaged in tendinopathy and OA. We also found 336 EV-miRNAs, 51 of which accounted for more than95% of the genetic message. A focused analysis based on miRNAs related to OA and tendinopathy showed that most abundant EV-miRNAs are teno- and chondro-protective, able to induce M2 macrophage polarization, inhibit inflammatory Tcells, and promote Treg. Functional analysis on IL-1beta treated tenocytes and chondrocytes resulted in downregulation of inflammation-associated genes. Overall, presence of key regulatory molecules and miRNAs explain the promising therapeutic results of hAMSCs and their secretome for treatment of musculoskeletal conditions and are a groundwork for similar studies in other pathologies. Furthermore, identified molecules will pave the way for future studies aimed at more sharply predicting disease-targeted clinical efficacy, as well as setting up potency and release assays to fingerprint clinical-grade batches of whole secretome or purified components