A versatile dual spot laser scanning confocal microscopy system for advanced fluorescence correlation spectroscopy analysis in living cell

A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and shape of the measurement volumes. A method that allows to monitor independently the excitation and collection efficiency distribution is proposed. Finally, a few examples of measurements that exploit the two spots in static and/or scanning schemes, are reported.Comment: Accepted for publication in Review of Scientific Instrumen

Harmonised Principles for Public Participation in Quality Assurance of Integrated Water Resources Modelling

The main purpose of public participation in integrated water resources modelling is to improve decision-making by ensuring that decisions are soundly based on shared knowledge, experience and scientific evidence. The present paper describes stakeholder involvement in the modelling process. The point of departure is the guidelines for quality assurance for `scientific` water resources modelling developed under the EU research project HarmoniQuA, which has developed a computer based Modelling Support Tool (MoST) to provide a user-friendly guidance and a quality assurance framework that aim for enhancing the credibility of river basin modelling. MoST prescribes interaction, which is a form of participation above consultation but below engagement of stakeholders and the public in the early phases of the modelling cycle and under review tasks throughout the process. MoST is a flexible tool which supports different types of users and facilitates interaction between modeller, manager and stakeholders. The perspective of using MoST for engagement of stakeholders e.g. higher level participation throughout the modelling process as part of integrated water resource management is evaluate

Geometrical effects on the optical properties of quantum dots doped with a single magnetic atom

The emission spectra of individual self-assembled quantum dots containing a single magnetic Mn atom differ strongly from dot to dot. The differences are explained by the influence of the system geometry, specifically the in-plane asymmetry of the quantum dot and the position of the Mn atom. Depending on both these parameters, one has different characteristic emission features which either reveal or hide the spin state of the magnetic atom. The observed behavior in both zero field and under magnetic field can be explained quantitatively by the interplay between the exciton-manganese exchange interaction (dependent on the Mn position) and the anisotropic part of the electron-hole exchange interaction (related to the asymmetry of the quantum dot).Comment: 5 pages, 5 figures, to be published in Phys. Rev. Let

Matter Mixing in Aspherical Core-collapse Supernovae: Three-dimensional Simulations with Single Star and Binary Merger Progenitor Models for SN 1987A

We perform three-dimensional hydrodynamic simulations of aspherical core-collapse supernovae focusing on the matter mixing in SN 1987A. The impacts of four progenitor (pre-supernova) models and parameterized aspherical explosions are investigated. The four pre-supernova models include a blue supergiant (BSG) model based on a slow merger scenario developed recently for the progenitor of SN 1987A (Urushibata et al. 2018). The others are a BSG model based on a single star evolution and two red supergiant (RSG) models. Among the investigated explosion (simulation) models, a model with the binary merger progenitor model and with an asymmetric bipolar-like explosion, which invokes a jetlike explosion, best reproduces constraints on the mass of high velocity $^{56}$Ni, as inferred from the observed [Fe II] line profiles. The advantage of the binary merger progenitor model for the matter mixing is the flat and less extended $\rho \,r^3$ profile of the C+O core and the helium layer, which may be characterized by the small helium core mass. From the best explosion model, the direction of the bipolar explosion axis (the strongest explosion direction), the neutron star (NS) kick velocity, and its direction are predicted. Other related implications and future prospects are also given

p-Type doping of II-VI heterostructures from surface states: application to ferromagnetic Cd1−x_{1-x}Mnx_xTe quantum wells

We present a study of p-type doping of CdTe and Cd$_{1-x}$Mn$_x$Te quantum wells from surface states. We show that this method is as efficient as usual modulation doping with nitrogen acceptors, and leads to hole densities exceeding $2 \times 10^{11}$ cm$^{-2}$. Surface doping was successfully applied to obtain carrier-induced ferromagnetism in a Cd$_{1-x}$Mn$_x$Te quantum well. The observed temperature dependence of photoluminescence spectra, and the critical temperature, correspond well to those previously reported for ferromagnetic quantum wells doped with nitrogen.Comment: 4 figure

Optical-fiber-microsphere for remote fluorescence correlation spectroscopy

International audienceFluorescence correlation spectroscopy (FCS) is a versatile method that would greatly benefit to remote optical-fiber fluorescence sensors. However, the current state-of-the-art struggles with high background and low detection sensitivities that prevent the extension of fiber-based FCS down to the single-molecule level. Here we report the use of an optical fiber combined with a latex microsphere to perform FCS analysis. The sensitivity of the technique is demonstrated at the single molecule level thanks to a photonic nanojet effect. This offers new opportunities for reducing the bulky microscope setup and extending FCS to remote or in vivo applications

Discrete complex analysis on planar quad-graphs

We develop a linear theory of discrete complex analysis on general quad-graphs, continuing and extending previous work of Duffin, Mercat, Kenyon, Chelkak and Smirnov on discrete complex analysis on rhombic quad-graphs. Our approach based on the medial graph yields more instructive proofs of discrete analogs of several classical theorems and even new results. We provide discrete counterparts of fundamental concepts in complex analysis such as holomorphic functions, derivatives, the Laplacian, and exterior calculus. Also, we discuss discrete versions of important basic theorems such as Green's identities and Cauchy's integral formulae. For the first time, we discretize Green's first identity and Cauchy's integral formula for the derivative of a holomorphic function. In this paper, we focus on planar quad-graphs, but we would like to mention that many notions and theorems can be adapted to discrete Riemann surfaces in a straightforward way. In the case of planar parallelogram-graphs with bounded interior angles and bounded ratio of side lengths, we construct a discrete Green's function and discrete Cauchy's kernels with asymptotics comparable to the smooth case. Further restricting to the integer lattice of a two-dimensional skew coordinate system yields appropriate discrete Cauchy's integral formulae for higher order derivatives.Comment: 49 pages, 8 figure

Light and electric field control of ferromagnetism in magnetic quantum structures

A strong influence of illumination and electric bias on the Curie temperature and saturation value of the magnetization is demonstrated for semiconductor structures containing a modulation-doped p-type Cd0.96Mn0.04Te quantum well placed in various built-in electric fields. It is shown that both light beam and bias voltage generate an isothermal and reversible cross-over between the paramagnetic and ferromagnetic phases, in the way that is predetermined by the structure design. The observed behavior is in quantitative agreement with the expectations for systems, in which ferromagnetic interactions are mediated by the weakly disordered two-dimensional hole liquid.Comment: 4 pages and 3 figure

Excitonic giant Zeeman effect in GaN:Mn^3+

We describe a direct observation of the excitonic giant Zeeman splitting in (Ga,Mn)N, a wide-gap III-V diluted magnetic semiconductor. Reflectivity and absorption spectra measured at low temperatures display the A and B excitons, with a shift under magnetic field due to s,p-d exchange interactions. Using an excitonic model, we determine the difference of exchange integrals between Mn^3+ and free carriers in GaN, N_0(alpha-beta)=-1.2 +/- 0.2 eV. Assuming a reasonable value of alpha, this implies a positive sign of beta which corresponds to a rarely observed ferromagnetic interaction between the magnetic ions and the holes.Comment: 4 pages, 4 figure

Ferromagnetic (Ga,Mn)N epilayers versus antiferromagnetic GaMn3_3N clusters

Mn-doped wurtzite GaN epilayers have been grown by nitrogen plasma-assisted molecular beam epitaxy. Correlated SIMS, structural and magnetic measurements show that the incorporation of Mn strongly depends on the conditions of the growth. Hysteresis loops which persist at high temperature do not appear to be correlated to the presence of Mn. Samples with up to 2% Mn are purely substitutional Ga$_{1-x}$Mn$_x$N epilayers, and exhibit paramagnetic properties. At higher Mn contents, precipitates are formed which are identified as GaMn$_3$N clusters by x-ray diffraction and absorption: this induces a decrease of the paramagnetic magnetisation. Samples co-doped with enough Mg exhibit a new feature: a ferromagnetic component is observed up to $T_c\sim175$ K, which cannot be related to superparamagnetism of unresolved magnetic precipitates.Comment: Revised versio