Espécies e biotipos de Campylobacter termotolerantes em reservatórios animais do sul do Chile

The prevalence of thermotolerant Campylobacters in mammals and birds from Southern Chile was determined. Campylobacters were isolated from 46.3% of the animals studied being C. jejuni biotipe 1 the most frequent (25.7%) followed by C. coli (17.4%) and C. jejuni biotipe 2 (3.2%).Foi determinada a prevalência de Campylobacter termotolerantes em mamíferos e aves do sul do Chile. Campylobacter foi isolado em 46,3% dos animais estudados sendo C. jejuni biotipo 1 o mais freqüente (25,7%) seguido de C. coli (17,4%) e C. jejuni biotipo 2 (3,2%)

Ocorrência de Campylobacter jejuni em crianças com diarréia e normais em São Paulo, Brasil

Campylobacter jejuni was looked for in the feaces of 262 children, beeing 189 with acute diarrhoea, 31 with chronic diarrhoea and 42 controls. The organism was isolated from 14 (7.4%), 2 (6.5%) and 6 (14.3%) of the children, and each group, respectively.Campylobacter jejuni foi pesquisado nas fezes de 262 crianças, sendo 189 com diarréia aguda, 31 com diarréia crônica e 42 sem sintomatologia gastrintestinal. A bactéria foi encontrada em 14 (7,4%), 2 (6,5%) e 6 (14,3%) das crianças, em cada um dos grupos, respectivamente

ocorrência de Campylobacter jejuni em animais do zoológico

Fifty fecal specimens from animals of the São Paulo City Zoo were cultured for Campylobacter jejuni. The organism was isolated from 4 out 20 primates and it was not found in the other mammals and birds assayed in this study.Foram estudadas 50 amostras de fezes obtidas de diversas espécies animais da Fundação Parque Zoológico de São Paulo. C. jejuni foi isolado em 4 dos 20 primatas estudados, não sendo encontrado nas fezes de outros mamíferos e aves ingressados neste estudo

Sacrococcygeal giant hemangiopericytoma: a rare presentation and management

Hemangiopericytoma is a tumor derivated from the mesenchymal cells that surround the capillary of blood vessels. It has a several anatomic localization and clinic scenarios, it´s presentation with a media in the 6th decade; an early diagnostic and treatment represents a better clinic scenario for the patient. The next article presents a case report of a 51 years old man with a tumor localized in the sacrococcygeal space, with big dimensions that limited his daily activities without other symptomatology, image studies with no evidence of distant progression. Surgical resection was performed, removing mass depending from mayor and minor gluteus, achieving an immediate reconstruction with a satisfactory aesthetic and functional result. The authors presented this rare case due to the lack of published information and how it can be treated to achieve good results

Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status

Objectives This study was aimed to assess the diversity of the meconium microbiome and determine if the bacterial community is affected by maternal diabetes status. Methods The first intestinal discharge (meconium) was collected from 23 newborns stratified by maternal diabetes status: 4 mothers had pre-gestational type 2 diabetes mellitus (DM) including one mother with dizygotic twins, 5 developed gestational diabetes mellitus (GDM) and 13 had no diabetes. The meconium microbiome was profiled using multi-barcode 16S rRNA sequencing followed by taxonomic assignment and diversity analysis. Results All meconium samples were not sterile and contained diversified microbiota. Compared with adult feces, the meconium showed a lower species diversity, higher sample-to-sample variation, and enrichment of Proteobacteria and reduction of Bacteroidetes. Among the meconium samples, the taxonomy analyses suggested that the overall bacterial content significantly differed by maternal diabetes status, with the microbiome of the DM group showing higher alpha-diversity than that of no-diabetes or GDM groups. No global difference was found between babies delivered vaginally versus via Cesarean-section. Regression analysis showed that the most robust predictor for the meconium microbiota composition was the maternal diabetes status that preceded pregnancy. Specifically, Bacteroidetes (phyla) and Parabacteriodes (genus) were enriched in the meconium in the DM group compared to the no-diabetes group. Conclusions Our study provides evidence that meconium contains diversified microbiota and is not affected by the mode of delivery. It also suggests that the meconium microbiome of infants born to mothers with DM is enriched for the same bacterial taxa as those reported in the fecal microbiome of adult DM patients

The Right to Code and Share Arms

Glycerol is, to date, the most widely used cryoprotectant to freeze stallion spermatozoa at concentrations between 2% and 5%. Cryoprotectant toxicity has been claimed to be the single most limiting factor for the success of cryopreservation. In order to evaluate the toxic effects of the concentrations of glycerol used in practice, stallion spermatozoa were incubated in Biggers Whitten and Whittingham (BWW) media supplemented with 0%, 0.5%, 1.5%, 2.5%, 3.5%, and 5% glycerol. In two additional experiments, a hyposmotic (75 mOsm/kg) and a hyperosmotic (900 mOsm/kg) control media were included. Sperm parameters evaluated included cell volume, membrane integrity, lipid peroxidation, caspase 3, 7, and 8 activation, mitochondrial membrane potential, and integrity of the cytoskeleton. Glycerol exerted toxicity at concentrations 3.5% and the maximal toxicity was observed at 5%. The actin cytoskeleton was especially sensitive to glycerol presence, inducing rapid F actin depolymerization at concentrations over 1.5%. The sperm membrane and the mitochondria were other structures affected. The toxicity of glycerol is apparently related to osmotic and nonosmotic effects. In view of our results the concentration of glycerol in the freezing media for stallion spermatozoa should not surpass 2.5%.Funding Agencies|Ministerio de Ciencia e Innovacion-FEDER Madrid, Spain|AGL 2010 20758 (GAN)|Inia|RZ2008-00018-00-00|Junta de Extremadura FEDER GR|10010

Cuidado de enfermería en adultos mayores con síndrome de distrés respiratorio agudo por covid-19

The Acute Respiratory Distress Syndrome is one of the most frequent complications caused by COVID-19, statistics kept by the Spanish Medical Society report that 33.8% of hospitalized patients present it, it is the most serious stage, injuring their lungs in addition to leading to to systemic complications which has caused 50% of mortality according to the World Health Organization, the initial reports of the presence of the virus in the city of Wuhan (China), reflected it as pneumonia, however it would later be recognized as Respiratory Syndrome Severe Acute Coronavirus 2 (SARS-CoV-2) The risks and complications in older adults increase due to the pathologies associated with this vulnerable population, with a higher incidence due to its transmission mechanism when an infected person exhales very small respiratory droplets and particles that contain the virus can be inhaled by other people or deposited on their mucous membranes Between the care of nursing staff are continuously monitoring stable vital signs, and guaranteeing the optimal oxygen supply to maintain saturations greater than 90%, it has been shown that the prone position will reduce total collapse of the lungs, and improve ventilatory perfusion, which is why they should to adhere to the protocols for the prevention of integumentary injuries to avoid pressure injury. Conclusions: the biggest complication of COVID-19 is diffuse alveolar damage and pulmonary fibrosis, which is affecting, however, therapeutic protocols have been established. The documentary review was carried out, which is a dynamic process that essentially consists of the collection, classification, recovery and distribution of information. Determine Nursing Care in older adults with Acute Respiratory Distress Syndrome (ARDS) by Covid-19.El Síndrome de Distrés Respiratorio Agudo , es de las complicaciones más frecuentes por  COVID-19 , estadísticas llevadas por la Sociedad Medica Española reportan que el 33,8% de los pacientes hospitalizados lo presentan,  es la etapa más grave lesionando sus pulmones además de conllevar a complicaciones  sistémicas lo cual ha causado 50% de  la mortalidad según la Organización Mundial de la Salud, los informes iniciales de la presencia del virus en la ciudad de Wuhan (China), le reflejaban como neumonías, sin embargo posteriormente es reconocería como Síndrome Respiratorio Agudo Severo del Coronavirus 2 (SARS-CoV-2) Los riesgos y complicaciones en adultos mayores incrementan debido a las patologías asociadas a esta población vulnerable, con mayor incidencia por su mecanismo de transmisión cuando una persona infectada exhala gotitas y partículas respiratorias   muy pequeñas que contienen el virus pueden ser inhaladas por otras personas o depositarse en sus mucosas Entre los cuidados de enfermería están el monitoreo continuo los signos vitales estables, y el garantizar el aporte de oxígeno óptimo para mantener saturaciones mayores 90% se ha evidenciado que la posición de decúbito prono  reducirá el colapso total de los pulmones, y mejorar la ventilo perfusión por lo cual deben de apegarse a los protocolos para la prevención de lesiones tegumentarias para evitar lesión  por presión. Conclusiones: la mayor complicación del COVID-19 es el daño difuso alveolar y la fibrosis pulmonar, lo cual está afectando sin embrago se han establecidos protocolos terapéuticos. Se hizo la revisión documental, el cual es un proceso dinámico que consiste esencialmente en la recogida, clasificación, recuperación y distribución de la información Determinar el Cuidado de Enfermería en adultos mayores con Síndrome de Distrés Respiratorio Agudo (SDRA) por Covid-19