190 research outputs found
Exploring Jahrom Medical University students' attitudes towards the Islamic self-evaluation in 2015
Introduction: Self- evaluation is the process of designing, building and maintaining human behaviors that can lead to maturity and perfection. Therefore, the aim of this study is to examine Jahrom Medical University students' attitudes towards the Islamic self-assessment in 2015.Method: This cross-sectional and descriptive study was carried out on the students of Jahrom University of Medical Sciences in 2015. Using purposive sampling, 223 samples were selected.Collection tool was Islamic self-assessment questionnaire in the form of 20 items based on the Likert scale consisting stipulation, calculating, meditation and expostulation. Data analysis was performed using statistical software SPSS 11 and paired T-test, ANOVA, Pearson correlation.Results: 223 students participated in this cross-sectional and descriptive study.68.2% were female and the rest were male. Their average age was 2.18 ± 20.4 in the range of 15 to 45 years.28.7 percent of students were medical field and 27.8 percent from the field of Anesthesiology.71.3% were undergraduates. The results of Pearson correlation coefficient showed that there was a significant correlation between the components of Islamic selfevaluation (stipulation, calculating, meditation, expostulation) and Islamic self-evaluation (P-value <0.05).Conclusion: The students of Jahrom University of Medical Sciences are in relatively good condition in terms of the four stages of Islamic self-evaluation.Keywords: Islamic self-evaluation, students, Jahrom University of Medical Science
The knowledge and the use of psychological skills of anger management skills at the bedside by the physicians and nurses of Jahrom University of Medical Sciences in 2013
Although all individuals could be exposed to the outburst of anger, a group of them are more at risk than others because of the nature of their work and responsibilities, that the physicians and nurses are placed in this group. In the field of psychology, different skills such as social protection systems, problem-solving skills, self-relaxation system, use of humor skills and communication and negotiation skills have been proposed to control anger. Given the importance of anger management in the prevention of job violence in the health environment and its impact on psychological health of psychiatrists and nurses and also the lack of information and research on the topic, this study aimed to study the knowledge and the use of psychological skills of anger management at the bedside by the physicians and nurses of Jahrom University of Medical Sciences.Methodology: This study is a cross-sectional and descriptive research, its studied sample included 259 general practitioners, specialists and nurses working in two general hospitals affiliated to Jahrom University of Medical Sciences. They were included in the study by the census method. The data was collected by the self-report method and two researcher-made questionnaires: a questionnaire of the knowledge of the psychological skills of anger management and a questionnaire of the use of anger management skills at the bedside and their validity (face and content) and reliability were examined before performing the research. Data analysis was performed with descriptive statistics (mean, frequency, percentage) using SPSS software.Results: The mean of the knowledge of the physicians and nurses of Jahrom University of Medical Sciences from the psychological skills of anger management was 30.2 (standard deviation is equal to 1.53) that based on the classification of the scores of related questionnaire, it was at a good level. Also, the results showed that the mean of the use of psychological skills of anger management at the bedside by the physicians and nurses working in Jahrom University of Medical Sciences was 39.54 (standard deviation is equal to 1.28) that based on the classification of the scores of related questionnaire, it was at a good level.Conclusion: According to the results, the knowledge and the use of psychological skills of anger management skills at the bedside by the physicians and nurses of Jahrom University of Medical Sciences were at the desired level, however, it is proposed to use other evaluation and assessment methods which have no limitations of self-report method in future studies.Keywords: anger management skill, nurses, physician
C3G mediated suppression of malignant transformation involves activation of PP2A phosphatases at the subcortical actin cytoskeleton
In previous work, we demonstrated that C3G suppresses Ras oncogenic transformation by a mechanism involving inhibition of ERK phosphorylation. Here we present evidences indicating that this suppression mechanism is mediated, at least in part, by serine/threonine phosphatases of the PP2A family. Thus: (i) ectopic expression of C3G or C3GΔCat (mutant lacking the GEF activity) increases specific ERK-associated PP2A phosphatase activities; (ii) C3G and PP2A interact, as demonstrated by immunofluorescence and co-immunoprecipitation experiments; (iii) association between PP2A and MEK or ERK increases in C3G overexpressing cells; (iv) phosphorylated-inactive PP2A level decreases in C3G expressing clones and, most importantly, (v) okadaic acid reverts the inhibitory effect of C3G on ERK phosphorylation. Moreover, C3G interacts with Ksr-1, a scaffold protein of the Ras-ERK pathway that also associates with PP2A. The fraction of C3G involved in transformation suppression is restricted to the subcortical actin cytoskeleton where it interacts with actin. Furthermore, the association between C3G and PP2A remains stable even after cytoskeleton disruption with cytochalasin D, suggesting that the three proteins form a complex at this subcellular compartment. Finally, C3G- and C3GΔCat-mediated inhibition of ERK phosphorylation is reverted by incubation with cytochalasin D. We hypothesize that C3G triggers PP2A activation and binding to MEK and ERK at the subcortical actin cytoskeleton, thus favouring ERK dephosphorylation. © 2007 Elsevier Inc. All rights reserved.This work was supported by grants SAF2003-04177 andGEN2003- 20239-C06-02 from MEC, Spain, FIS-FEDERPI030651, PI041324 and PI061274 from ISCIII,MSC, Spain, as well as institutional support from Redes Temáticas (C03/10 and RD06/0020/0000) de investigación en cáncer from ISCIII, MSC, Spain. S. M-E is a postodoctoral fellow supported by grant GEN2003-20239-C06-02. C.G. was supported by the Ramón y Cajal Program from the Spanish Ministry of Education.Peer Reviewe
Recombinant forms of Leishmania amazonensis excreted/secreted promastigote surface antigen (PSA) induce protective immune responses in dogs
International audiencePreventive vaccination is a highly promising strategy for interrupting leishmaniasis transmission that can, additionally, contribute to elimination. A vaccine formulation based on naturally excreted secreted (ES) antigens was prepared from L. infantum promastigote culture supernatant. This vaccine achieved successful results in Phase III trials and was licensed and marketed as CaniLeish. We recently showed that newly identified ES promastigote surface antigen (PSA), from both viable promastigotes and axenically-grown amastigotes, represented the major constituent and the highly immunogenic antigen of L. infantum and L. amazonensis ES products. We report here that three immunizations with either the recombi-nant ES LaPSA-38S (rPSA) or its carboxy terminal part LaPSA-12S (Cter-rPSA), combined with QA-21 as adjuvant, confer high levels of protection in naive L. infantum-infected Beagle dogs, as checked by bone marrow parasite absence in respectively 78.8% and 80% of vaccinated dogs at 6 months post-challenge. The parasite burden in infected vaccinated dogs was significantly reduced compared to placebo group, as measured by q-PCR. Moreover, our results reveal humoral and cellular immune response clear-cut differences between vaccinated and control dogs. An early increase in specific IgG2 antibodies was observed in rPSA/QA-21-and Cter-rPSA/QA-21-immunized dogs only. They were found functionally active in vitro and were highly correlated with vaccine protection. In vaccinated protected dogs, IFN-Îł and NO productions, as well as anti-leishmanial macrophage activity, were increased. These data strongly suggest that ES PSA or its carboxy-terminal part, in recom-binant forms, induce protection in a canine model of zoonotic visceral leishmaniasis by inducing a Th1-dominant immune response and an appropriate specific antibody response. These data suggest that they could be considered as important active components in vaccine candidates
C-Terminal Domain Deletion Enhances the Protective Activity of cpa/cpb Loaded Solid Lipid Nanoparticles against Leishmania major in BALB/c Mice
Cutaneous leishmaniasis (CL) is the most common form of leishmaniasis with an annual incidence of approximately 2 million cases and is endemic in 88 countries, including Iran. CL's continued spread, along with rather ineffectual treatments and drug-resistant variants emergence has increased the need for advanced preventive strategies. We studied Type II cysteine proteinase (CPA) and Type I (CPB) with its C-terminal extension (CTE) as cocktail DNA vaccine against murine and canine leishmaniasis. However, adjuvants' success in enhancing immune responses to selected antigens led us to refocus our vaccine development programs. Herein, we discuss cationic solid lipid nanoparticles' (cSLN) ability to improve vaccine-induced protective efficacy against CL and subsequent lesion size and parasite load reduction in BALB/c mice. For this work, we evaluated five different conventional as well as novel parasite detection techniques, i.e., footpad imaging, footpad flowcytometry and lymph node flowcytometry for disease progression assessments. Vaccination with cSLN-cpa/cpb-CTE formulation showed highest parasite inhibition at 3-month post vaccination. Immunized mice showed reduced IL-5 level and significant IFN-ĂŁ increase, compared to control groups. We think our study represents a potential future and a major step forward in vaccine development against leishmaniasis
Research Priorities for Neglected Infectious Diseases in Latin America and the Caribbean Region
Dujardin, J. C. et al. 5 p.-1 tab.Global priorities for research in neglected
infectious diseases (NIDs) can be assessed
in different ways, but it is important to
realize that regional priorities may significantly
differ one from another. The region
of Latin America and the Caribbean (LAC)
is—along with Africa and Asia—more
affected by NIDs than other regions of the
world. Some of the Latin American NIDs
are common to other continents, while
others are very specific or disproportionately
affect the Latin American region [1–
3] (Table 1). Because of its huge ecological
diversity, ongoing environmental changes,
and massive migrations, LAC is also a
catalyst for the (re-)emergence and spreading
of NIDs, both inside and outside the
subcontinent. Following a colloquium on
NIDs in LAC held in Lima, Peru, between
12 and 14 November 2009, a thematic
workshop was organized with the support
of the European Commission (EC). It
involved 29 scientists (16 from the Americas,
two from the Democratic Republic of
Congo and India, respectively, and nine
from Europe) working on different NIDs
and representing several research areas
from basic to applied. This report summarizes
the consensus comments of the expert
group after oral and written consultation. It
is envisaged that this document should
stimulate a debate within the scientific
community and serve as a recommendation
for future actions by international or
regional funding agencies in the area of
NIDs in LACThis work was supported by the Directorate-General for Development Cooperation of the Belgian Government (framework agreement 03, project 95502) and the European CommissionPeer reviewe
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