Interactions of SARS Coronavirus Nucleocapsid Protein with the host cell proteasome subunit p42

<p>Abstract</p> <p>Background</p> <p>Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spreads rapidly and has a high case-mortality rate. The nucleocapsid protein (NP) of SARS-CoV may be critical for pathogenicity. This study sought to discover the host proteins that interact with SARS-CoV NP.</p> <p>Results</p> <p>Using surface plasmon resonance biomolecular interaction analysis (SPR/BIA) and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, we found that only the proteasome subunit p42 from human fetal lung diploid fibroblast (2BS) cells bound to SARS-CoV NP. This interaction was confirmed by the glutathione S-transferase (GST) fusion protein pulldown technique. The co-localization signal of SARS-CoV NP and proteasome subunit p42 in 2BS cells was detected using indirect immunofluorescence and confocal microscopy. p42 is a subunit of the 26S proteasome; this large, multi-protein complex is a component of the ubiquitin-proteasome pathway, which is involved in a variety of basic cellular processes and inflammatory responses.</p> <p>Conclusion</p> <p>To our knowledge, this is the first report that SARS-CoV NP interacts with the proteasome subunit p42 within host cells. These data enhance our understanding of the molecular mechanisms of SARS-CoV pathogenicity and the means by which SARS-CoV interacts with host cells.</p

Following palladium catalyzed methoxycarbonylation by hyperpolarized NMR spectroscopy : A: para hydrogen based investigation

Pd(OTf)2(bcope) is shown to react in methanol solution with diphenylacetylene, carbon monoxide and hydrogen to produce the methoxy-carbonylation product methyl 2,3 diphenyl acrylate alongside cis- and trans-stilbene. In situ NMR studies harnessing the parahydrogen induced polarization effect reveal substantially enhanced 1H NMR signals in both protic and aprotic solvents for a series of reaction intermediates that play a direct role in this homogeneous transformation. Exchange spectroscopy (EXSY) measurements reveal that the corresponding CO adducts are less reactive than their methanol counterparts

Using Parahydrogen Induced Polarization to Study Steps in the Hydroformylation Reaction.

A range of iridium complexes, Ir(η3-C3H5)(CO)(PR2R’)2 (1a-1e) [where 1a, PR2R’ = PPh3, 1b P(p-tol)3, 1c PMePh2, 1d PMe2Ph and 1e PMe3] were synthesized and their reactivity as stoichiometric hydroformylation precursors studied. Para-hydrogen assisted NMR spectroscopy detected the following intermediates: Ir(H)2(η3-C3H5)(CO)(PR2R’) (2a-e), Ir(H)2(η1-C3H5)(CO)(PR2R’)2 (4d-e), Ir(H)2(η1-C3H5)(CO)2(PR2R’) (10a-e), Ir(H)2(CO-C3H5)(CO)2(PR2R’) (11a-c), Ir(H)2(CO-C3H7)(CO)2(PR2R’) (12a-c) and Ir(H)2(CO-C3H5)(CO)(PR2R’)2 (13d-e). Some of these species exist as two geometric isomers according to their multinuclear NMR characteristics. The NMR studies suggest a role for the following 16 electron species in these reactions: Ir(η3-C3H5)(CO)(PR2R’), Ir(η1-C3H5)(CO)(PR2R’)2, Ir(η1-C3H5)(CO)2(PR2R’), Ir(CO-C3H5)(CO)2(PR2R’), Ir(CO-C3H7)(CO)2(PR2R’) and Ir(CO-C3H5)(CO)(PR2R’)2. Their role is linked to several 18 electron species in order to confirm the route by which hydroformylation and hydrogenation proceeds

Seasonal, Diurnal and Wind-Direction-Dependent Variations of the Aerodynamic Roughness Length in Two Typical Forest Ecosystems of China

Aerodynamic roughness length (zom) is an important parameter for reliably simulating surface fluxes. The parameter varies with wind speed, atmospheric stratification, terrain and other factors; however, variations of this parameter are not properly considered in most models, which may result in uncertainties in simulating surface latent heat and sensible heat flux. There have been few studies of the diurnal and wind-direction dependent variations in zom. This study analyzes the seasonal, diurnal and wind-direction-dependent variations in zom calculated from the profile of meteorological data for two forest systems of China, and explores the mechanism underlying these variations

Characteristics of soil moisture under different vegetation coverage in Horqin Sandy Land, northern China.

Vegetation restoration as an effective sand fixation measure has made great achievements in China. However, soil water conditions deteriorate with the development and maturity of sand-fixing vegetation. In this study, we investigated the relationship between soil water content (SWC) and vegetation coverage (VC) at different portion (top, middle and bottom) on ten sand dunes during the growing season (April to October) in Horqin Sandy Land, northern China. We analyzed the temporal and spatial variation characteristics of SWC under different VC. The results indicate that VC and soil water storage were negatively correlated. The effect of vegetation on soil water storage on the sand dunes was greater in the dry season than the wet season. The VC and coefficient of coefficient of SWC were positively correlated at the 20 to 140 cm soil depth. As VC increased, the effect of drought stress increased at the 20 to 200 cm soil depths. The VC and SWC were negatively correlated at all the three portions of the sand dunes. According to soil water conditions and the concept of wilting humidity at different VC, we found that the suitable VC values were less than 0.46, 0.52, and 0.71 at the top, middle and bottom of the sand dunes, respectively