DON BENITO PÉREZ GALDÓS [Material gráfico]

Copia digital. Madrid : Ministerio de Educación, Cultura y Deporte. Subdirección General de Coordinación Bibliotecaria, 201

In situ assessment and minimization of nonlinear propagation effects for femtosecond-laser waveguide writing in dielectrics

The effect of nonlinear propagation on the shape of the focal volume has been assessed by in situ plasma emission imaging during the subsurface processing of a commercial phosphate glass. The sample was processed with an elliptically shaped femtosecond-laser beam at 1 kHz repetition rate and scanned transversely with respect to the writing beam axis. As a consequence, optimal conditions for minimizing undesirable nonlinear propagation effects during the production of optical waveguides by direct laser writing have been determined. Under these conditions, it is possible to induce structural transformations and still preserve the focal volume shape associated with the linear propagation regime. While at low pulse energy a single scan laser-written structure does not support a guided mode, the use of multiple scans with minimized nonlinear propagation effects enables the production of optical waveguides. The latter show a significantly improved performance in terms of the refractive index change and propagation losses when compared to single scan waveguides. © 2010 Optical Society of America.This work was partially supported by the University of Zaragoza under Project 223/88 and by the Spanish Ministry of Science and Innovation under TEC2008-01183 project. A. Ruiz de la Cruz and W. Gawelda acknowledge their I3P-CSIC postdoctoral contracts (co-funded by the European Social Fund). D. Puerto and A. Ferrer acknowledge their grants under Projects TEC 2005-00074 and TEC 2006-04538.Peer Reviewe

Optimización estadística de un bioproceso de ácido láctico a partir de lactosuero

The use of industrial waste as a substrate is a worldwide trend, whey is one of them. The objective of the research was to find the combination of temperature and pH that maximizes the productivity of lactic acid with the use of whey and Lactobacillus casei. The temperature variables were evaluated in the values ​​of (29, 31.1, 37, 42.7 and 45 °C) and pH (4.8, 5, 5.5, 6 and 6.2) to find optimal parameters of the bioprocess that maximize the volumetric productivity of lactic acid. by using the Central Composite Design using the Design Expert 11.0 program. Thirteen runs were carried out. The validity of the quadratic model was verified through 3 repetitions with the optimal parameters suggested by the program, and it was determined that the conditions for maximizing volumetric productivity are 40.2 °C of temperature and 5.6 of pH, reaching values ​​of 1.2 g. l-1h-1 and it was confirmed that there is no significant difference (p ≤ 0.05) with the values ​​provided by the model. Concluding that the optimal values ​​of temperature and pH found were close to those reported by other authors and it was shown that whey has great potential to be used as a substrate.El uso de residuales de la industria como sustrato es tendencia a nivel mundial, el lactosuero es uno de ellos. El objetivo de la investigación fue encontrar la combinación de temperatura y pH que maximicen la productividad de ácido láctico con el uso de lactosuero y Lactobacillus casei. Se evaluaron las variables de temperatura en los valores de (29, 31.1, 37, 42.7 y 45 °C) y pH (4.8, 5, 5.5, 6 y 6.2) para encontrar parámetros óptimos del bioproceso que maximicen la productividad volumétrica de ácido láctico mediante el uso del Diseño Central Compuesto utilizando el programa Design Expert 11.0. Se realizaron trece corridas. Se verificó la validez del modelo cuadrático mediante 3 repeticiones con los parámetros óptimos sugeridos por el programa y se determinó que las condiciones de maximización de la productividad volumétrica son 40.2 °C de temperatura y 5.6 de pH, alcanzando valores de 1.2 gl-1h-1 y se confirmó que no existe diferencia significativa (p ≤ 0.05) con los valores brindados por el modelo. Concluyendo que los valores óptimos de temperatura y pH encontrados fueron cercanos a los reportados por otros autores y se demostró que el lactosuero tiene un gran potencial para ser utilizado como sustrato

Daratumumab displays in vitro and in vivo anti-tumor activity in models of B-cell non-Hodgkin lymphoma and improves responses to standard chemo-immunotherapy regimens

Altres ajuts: This work was carried out at the Esther Koplowitz Center, Barcelona. Genmab and Janssen pharmaceuticals funded this research. Additional grants that contributed to this work included: [...], and CIBERONC (CB16/12/00334 and CB16/12/00225).CD38 is expressed in several types of non-Hodgkin lymphoma (NHL) and constitutes a promising target for antibody-based therapy. Daratumumab (Darzalex) is a first-in-class anti-CD38 antibody approved for the treatment of relapsed/refractory (R/R) multiple myeloma (MM). It has also demonstrated clinical activity in Waldenström macroglobulinaemia and amyloidosis. Here, we have evaluated the activity and mechanism of action of daratumumab in preclinical in vitro and in vivo models of mantle cell lymphoma (MCL), follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL), as monotherapy or in combination with standard chemo-immunotherapy. In vitro, daratumumab engages Fc-mediated cytotoxicity by antibody-dependent cell cytotoxicity and antibody-dependent cell phagocytosis in all lymphoma subtypes. In the presence of human serum, complement-dependent cell cytotoxicity was marginally engaged. We demonstrated by Selective Plane Illumination Microscopy that daratumumab fully penetrated a three-dimensional (3D) lymphoma organoid and decreased organoid volume. In vivo, daratumumab completely prevents tumor outgrowth in models of MCL and FL, and shows comparable activity to rituximab in a disseminated in vivo model of blastic MCL. Moreover, daratumumab improves overall survival (OS) in a mouse model of transformed CD20 FL, where rituximab showed limited activity. Daratumumab potentiates the antitumor activity of CHOP and R-CHOP in MCL and FL xenografts. Furthermore, in a patient-derived DLBCL xenograft model, daratumumab anti-tumor activity was comparable to R-CHOP and the addition of daratumumab to either CHOP or R-CHOP led to full tumor regression. In summary, daratumumab constitutes a novel therapeutic opportunity in certain scenarios and these results warrant further clinical development

Automatic simultaneous measurement of phase velocity and thickness in composite plates using iterative deconvolution

A new method for the automatic and simultaneous measurement of phase velocity and thickness for thin composite plates was developed based on Ping He's method, without any need of a priori knowledge of the material parameters. Two composites were analyzed: a block of clean epoxy and a thin specimen of glass-fiber reinforced plastic produced by resin transfer molding. The proposed method combines cross-correlation functions and iterative deconvolution for accurate measurement of times of flight and gating. The new method has demonstrated to be more accurate than conventional Ping He's method, and can be implemented automatically thus saving processing time and increasing accuracy.This research was funded by a Project IN-SMART, Grant no. VP1-3.1SMM-10-V-02-012 and by the Spanish Ministerio de Ciencia e Innovacion (TEC2011-23403).Rodriguez Martinez, A.; Svilainis, L.; Dumbrava, V.; Chaziachmetovas, A.; Salazar Afanador, A. (2014). Automatic simultaneous measurement of phase velocity and thickness in composite plates using iterative deconvolution. NDT and E International. 66:117-127. https://doi.org/10.1016/j.ndteint.2014.06.001S1171276

Zinc Phthalocyanine−Graphene Hybrid Material for Energy Conversion: Synthesis, Characterization, Photophysics and Photoelectrochemical Cell Preparation

Graphene exfoliation upon tip sonication in o-­‐DCB was accomplished. Then, covalent grafting of (2-­‐ aminoethoxy)(tri-­‐tert-­‐butyl) zinc phthalocyanine (ZnPc), to exfoliated graphene sheets was achieved. The newly formed ZnPc-­‐graphene hybrid material was found soluble in common organic solvents without any precipitation for several weeks. Application of diverse spectroscopic techniques verified the successful formation of ZnPc-­‐graphene hybrid materi-­‐ al, while thermogravimetric analysis revealed the amount of ZnPc loading onto graphene. Microscopy analysis based on AFM and TEM was applied to probe the morphological characteristics and to investigate the exfoliation of graphene sheets. Efficient fluorescence quenching of ZnPc in the ZnPc-­‐graphene hybrid material suggested that photoinduced events occur from the photoexcited ZnPc to exfoliated graphene. The dynamics of the photoinduced electron transfer was evaluated by femtosecond transient absorption spectroscopy, thus, revealing the formation of transient species such as ZnPc+ yielding the charge-­‐separated state ZnPc•+–graphene•–. Finally, the ZnPc-­‐graphene hybrid material was integrated into a photoactive electrode of an optical transparent electrode (OTE) cast with nanostructured SnO2 films (OTE/SnO2), which exhibited sta le and reproducible photocurrent responses and the incident photon-­‐to-­‐current conversion efficien-­‐ cy was determine