35 research outputs found
The Functional Consequences of Variation in Transcription Factor Binding
One goal of human genetics is to understand how the information for precise
and dynamic gene expression programs is encoded in the genome. The interactions
of transcription factors (TFs) with DNA regulatory elements clearly play an
important role in determining gene expression outputs, yet the regulatory logic
underlying functional transcription factor binding is poorly understood. Many
studies have focused on characterizing the genomic locations of TF binding, yet
it is unclear to what extent TF binding at any specific locus has functional
consequences with respect to gene expression output. To evaluate the context of
functional TF binding we knocked down 59 TFs and chromatin modifiers in one
HapMap lymphoblastoid cell line. We then identified genes whose expression was
affected by the knockdowns. We intersected the gene expression data with
transcription factor binding data (based on ChIP-seq and DNase-seq) within 10
kb of the transcription start sites of expressed genes. This combination of
data allowed us to infer functional TF binding. On average, 14.7% of genes
bound by a factor were differentially expressed following the knockdown of that
factor, suggesting that most interactions between TF and chromatin do not
result in measurable changes in gene expression levels of putative target
genes. We found that functional TF binding is enriched in regulatory elements
that harbor a large number of TF binding sites, at sites with predicted higher
binding affinity, and at sites that are enriched in genomic regions annotated
as active enhancers.Comment: 30 pages, 6 figures (7 supplemental figures and 6 supplemental tables
available upon request to [email protected]). Submitted to PLoS
Genetic
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Single-cell ATAC-Seq in human pancreatic islets and deep learning upscaling of rare cells reveals cell-specific type 2 diabetes regulatory signatures
Objective: Type 2 diabetes (T2D) is a complex disease characterized by pancreatic islet dysfunction, insulin resistance, and disruption of blood glucose levels. Genome-wide association studies (GWAS) have identified > 400 independent signals that encode genetic predisposition. More than 90% of associated single-nucleotide polymorphisms (SNPs) localize to non-coding regions and are enriched in chromatin-defined islet enhancer elements, indicating a strong transcriptional regulatory component to disease susceptibility. Pancreatic islets are a mixture of cell types that express distinct hormonal programs, so each cell type may contribute differentially to the underlying regulatory processes that modulate T2D-associated transcriptional circuits. Existing chromatin profiling methods such as ATAC-seq and DNase-seq, applied to islets in bulk, produce aggregate profiles that mask important cellular and regulatory heterogeneity. Methods: We present genome-wide single-cell chromatin accessibility profiles in >1,600 cells derived from a human pancreatic islet sample using single-cell combinatorial indexing ATAC-seq (sci-ATAC-seq). We also developed a deep learning model based on U-Net architecture to accurately predict open chromatin peak calls in rare cell populations. Results: We show that sci-ATAC-seq profiles allow us to deconvolve alpha, beta, and delta cell populations and identify cell-type-specific regulatory signatures underlying T2D. Particularly, T2D GWAS SNPs are significantly enriched in beta cell-specific and across cell-type shared islet open chromatin, but not in alpha or delta cell-specific open chromatin. We also demonstrate, using less abundant delta cells, that deep learning models can improve signal recovery and feature reconstruction of rarer cell populations. Finally, we use co-accessibility measures to nominate the cell-specific target genes at 104 non-coding T2D GWAS signals. Conclusions: Collectively, we identify the islet cell type of action across genetic signals of T2D predisposition and provide higher-resolution mechanistic insights into genetically encoded risk pathways. Published by Elsevier GmbH.Open access journalThis item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]
The combination of a genome-wide association study of lymphocyte count and analysis of gene expression data reveals novel asthma candidate genes
Recent genome-wide association studies (GWAS) have identified a number of novel genetic associations with complex human diseases. In spite of these successes, results from GWAS generally explain only a small proportion of disease heritability, an observation termed the ‘missing heritability problem’. Several sources for the missing heritability have been proposed, including the contribution of many common variants with small individual effect sizes, which cannot be reliably found using the standard GWAS approach. The goal of our study was to explore a complimentary approach, which combines GWAS results with functional data in order to identify novel genetic associations with small effect sizes. To do so, we conducted a GWAS for lymphocyte count, a physiologic quantitative trait associated with asthma, in 462 Hutterites. In parallel, we performed a genome-wide gene expression study in lymphoblastoid cell lines from 96 Hutterites. We found significant support for genetic associations using the GWAS data when we considered variants near the 193 genes whose expression levels across individuals were most correlated with lymphocyte counts. Interestingly, these variants are also enriched with signatures of an association with asthma susceptibility, an observation we were able to replicate. The associated loci include genes previously implicated in asthma susceptibility as well as novel candidate genes enriched for functions related to T cell receptor signaling and adenosine triphosphate synthesis. Our results, therefore, establish a new set of asthma susceptibility candidate genes. More generally, our observations support the notion that many loci of small effects influence variation in lymphocyte count and asthma susceptibility
Integrative genomics approaches to understanding the role of gene regulation in human evolution, disease, and cellular networks: A triptych
Human development and health involves the complex and coordinated regulation of gene expression across diverse tissues. Gene regulation is therefore an essential process in human biology. In the field of human genetics, this has only become more apparent as genomic technologies have made genome-wide surveys of genetic variation underlying human traits possible. In my thesis work, I studied the impact of variation in gene regulation on human traits from three distinct perspectives of human genetics. I first examined the contribution of gene regulation to human disease susceptibility by combining gene expression data with a genome-wide association study to identify novel asthma susceptibility candidate genes. I then studied the effects of depleting specific transcription factors from the cell on downstream gene expression by incorporating gene expression data (following cellular depletion of those factors) with genomic transcription factor binding data. Finally, I considered the role of gene regulation in human evolution by integrating RNA-seq data collected in human, chimpanzee, and rhesus macaque lymphoblastoid cell lines with promoter reporter assays conducted in the same lines. Throughout this work, I have synthesized multiple genomic data sets and multiple distinct sub-disciplines of human genetics in order to arrive at a unified view of the role of gene regulation in determining human traits
Functional Transcription Factor Binding Associated with More Overall Factor Binding at Target Genes
<p>These figures were generated in response to comments on a manuscript we had posted to arXiv and Haldane's Sieve. The paper is now published by PLoS Genetics.</p
arXiv Supplemental Figure 2
<p>In this figure, genes are binned into 20 equally sized bins based on the amount of transcription factor binding in the regulatory region of the gene (x-axis). For each bin, the fraction of genes that are differentially expressed in at least 1 knockdown (black), at least 2 knockdowns (red), at least 5 knockdowns (green), at least 10 knockdowns (blue), or at least 20 knockdowns (light blue) is calculated. Genes with more overall binding are more likely to be differentially expressed in knockdown experiments.</p
arXiv Supplemental Figure 1
<p>In this figure, the distribution of the number of binding events for bound genes differentially expressed in the knockdown experiment ("functionally bound") vs bound genes NOT differentially expressed in the knockdown ("non-functionally bound") is displayed for each knockdown experiment. The boxplots are sorted by the overall fraction of bound genes that are differentially expressed. Experiments with more functional binding have more overall binding.</p
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The Functional Consequences of Variation in Transcription Factor Binding
One goal of human genetics is to understand how the information for precise and dynamic gene expression programs is encoded in the genome. The interactions of transcription factors (TFs) with DNA regulatory elements clearly play an important role in determining gene expression outputs, yet the regulatory logic underlying functional transcription factor binding is poorly understood. Many studies have focused on characterizing the genomic locations of TF binding, yet it is unclear to what extent TF binding at any specific locus has functional consequences with respect to gene expression output. To evaluate the context of functional TF binding we knocked down 59 TFs and chromatin modifiers in one HapMap lymphoblastoid cell line. We then identified genes whose expression was affected by the knockdowns. We intersected the gene expression data with transcription factor binding data (based on ChIP-seq and DNase-seq) within 10 kb of the transcription start sites of expressed genes. This combination of data allowed us to infer functional TF binding. Using this approach, we found that only a small subset of genes bound by a factor were differentially expressed following the knockdown of that factor, suggesting that most interactions between TF and chromatin do not result in measurable changes in gene expression levels of putative target genes. We found that functional TF binding is enriched in regulatory elements that harbor a large number of TF binding sites, at sites with predicted higher binding affinity, and at sites that are enriched in genomic regions annotated as “active enhancers.”</p
ASHG 2013 Poster - The Functional Consequences of Variation in Transcription Factor Binding
<p>Poster presented at ASHG 2013 in Boston on work that was subsequently published in PLoS Genetics.</p
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Genome-Wide Association Studies of the Human Gut Microbiota
The bacterial composition of the human fecal microbiome is influenced by many lifestyle factors, notably diet. It is less clear, however, what role host genetics plays in dictating the composition of bacteria living in the gut. In this study, we examined the association of ~200K host genotypes with the relative abundance of fecal bacterial taxa in a founder population, the Hutterites, during two seasons (n = 91 summer, n = 93 winter, n = 57 individuals collected in both). These individuals live and eat communally, minimizing variation due to environmental exposures, including diet, which could potentially mask small genetic effects. Using a GWAS approach that takes into account the relatedness between subjects, we identified at least 8 bacterial taxa whose abundances were associated with single nucleotide polymorphisms in the host genome in each season (at genome-wide FDR of 20%). For example, we identified an association between a taxon known to affect obesity (genus Akkermansia) and a variant near PLD1, a gene previously associated with body mass index. Moreover, we replicate a previously reported association from a quantitative trait locus (QTL) mapping study of fecal microbiome abundance in mice (genus Lactococcus, rs3747113, P = 3.13 x 10−7). Finally, based on the significance distribution of the associated microbiome QTLs in our study with respect to chromatin accessibility profiles, we identified tissues in which host genetic variation may be acting to influence bacterial abundance in the gut