Political power and civil counterpower: the complex dynamics of the Catalan independence movement

Civil pro-independence associations are playing an increasingly significant role in Catalonia’s independence movement, putting pressure on the Catalan government to work decisively towards independence. This has led many Catalans to characterize the movement as a "bottom-up" phenomenon. This contribution analyses the shifting power dynamics within mainstream Catalan nationalism over the last decade, challenging simplistic notions of "top-down" and "bottom-up" pressures for change. In fact, the movement is now inherently multidimensional – partly thanks to the pivotal role of the cultural elites and their grasp of the power of new media – and this in itself accounts in large part for its success

Searching for orgasms in the dictionary: language literature and emotion in Lultim patriarca by Najat El Hachmi

L’últim patriarca by Najat El Hachmi (2008) has been critically acclaimed for its portrayal of the difficulties faced by a young Moroccan girl struggling to reconcile her past and present identities under the authoritarian yet unpredictable control of her father. This article focuses on the triangular relationship between language, literature, and emotion that underpins the protagonist’s quest for independence, self-esteem, and emotional maturity. Drawing on research on bilingualism and emotion, literature and emotion, and emotions and moral judgement, the article examines how these interconnecting themes are explored in the novel and their importance for our understanding of the protagonist’s journey towards personal autonomy. Two classic Catalan novels, Solitud by Victor Català and La plaça del Diamant by Mercè Rodoreda, provide the protagonist both with an insight into her own situation and a way to become the narrator of her own life story, thus (it appears) taking control of her own destiny. The article argues that this is only possible thanks to her ability to express her story in a new language, to rediscover the connection between bodily sensation and emotion, and to forge a new moral identity that is no longer conditioned by that of her hypocritical father

Enhanced Enzyme Performance by DNA Shuffling

In the quest for enhanced enzyme performance in non-natural applications, directed evolution emulates natural processes for the generation of diversity and selection of desirable traits. Classical methods for improving protein characteristics rely upon point mutation or cassette mutagenesis of a selected region within a desired sequence (i.e., focusing on a narrow sequence space). However, computer simulations of the evolution of linear sequences have demonstrated the importance of recombination of blocks of related sequences rather than sequence mutation alone. DNA shuffling includes in vivo and in vitro methods for recombination of nucleic acid sequences. In one format, DNA shuffling involves methods for in vitro homologous recombination of pools of related genes. For example, fragmentation of nucleic acid sequences that encode genes, can be followed by reassembly using the polymerase chain reaction (PCR). As the complete gene sequence can be shuffled, a wider sequence space can be accessed in searching for the desired, improved variant. Repeated cycles of recombination, optionally together with error-prone PCR to introduce point mutations, allow efficient molecular evolution of complex sequences in vitro, to provide biological molecules with improved properties

Achimota Pararubulavirus 3: A New Bat-Derived Paramyxovirus of the Genus Pararubulavirus

Bats are an important source of viral zoonoses, including paramyxoviruses. The paramyxoviral Pararubulavirus genus contains viruses mostly derived from bats that are common, diverse, distributed throughout the Old World, and known to be zoonotic. Here, we describe a new member of the genus Achimota pararubulavirus 3 (AchPV3) and its isolation from the urine of African straw-coloured fruit bats on primary bat kidneys cells. We sequenced and analysed the genome of AchPV3 relative to other Paramyxoviridae, revealing it to be similar to known pararubulaviruses. Phylogenetic analysis of AchPV3 revealed the failure of molecular detection in the urine sample from which AchPV3 was derived and an attachment protein most closely related with AchPV2—a pararubulavirus known to cause cross-species transmission. Together these findings add to the picture of pararubulaviruses, their sources, and variable zoonotic potential, which is key to our understanding of host restriction and spillover of bat-derived paramyxoviruses. AchPV3 represents a novel candidate zoonosis and an important tool for further study

Orphaning Regimes: The Missing Link Between Flattened and Penetrating Slab Morphologies

Slab orphaning is a newly discovered phenomenological behavior, where the slab tip breaks off at the top of the lower mantle (~660 km depth) and is abandoned by its parent slab. Upon orphaning, subduction continues uninterrupted through the lateral motion of the parent slab above 660 km depth. In this work, we present a regime diagram for the range of conditions under which slabs can orphan at the top of the lower mantle. Our models show that a viscosity jump at 1,000 km depth not coincident with the endothermic phase change responsible for the 660 km seismic discontinuity, is necessary for orphaning as is the presence of a low viscosity channel between 660 and 1,000 km depth. We show that orphan slabs, similar to other deep slab morphologies, can be the end result for a wide range of physical parameters governing slab dynamics: slab orphaning persists across wide variations in slab dip, slab yield stress/strength, Clapeyron slope values, and overriding plate nature. The diversity in orphan slab sizes and orphaning periods is tied to the orphaning regime space, which describes a hitherto unexplored region between deflected and penetrating deep-subduction modes. Orphaning provides a simple dynamic link between the well-known deflection and penetration, and provides one possible way for slabs to switch from direct penetration to deflection, littering the mantle with abandoned fragments. Orphan slabs are therefore the intermediary between these two extensively studied slab morphologies

Effect of Fabric Reinforcement on the Flexural Properties of EPS-Core Surfboard Constructions

Effect of Fabric Reinforcement on the Flexural Properties of EPS-Core Surfboard Construction

Display of E. coli Alkaline Phosphatase pIII or pVIII Fusions on Phagemid Surfaces Reveals Monovalent Decoration with Active Molecules

Active alkaline phosphatase of Escherichia coli (PhoA, EC 3.1.3.1) was displayed via the leucine zipper element of the Jun-Fos heterodimer on the surface of filamentous phage and the kinetic parameters Km and kcat were determined. The phoA gene was cloned downstream of fos while jun was inserted upstream of pIII or pVIII, alternatively, in the pJuFo phagemid vector. Both fusion genes are regulated by independent lacZ promoters. PhoA displayed on the phagemid pIII surface exhibited a Km of 11.2 µM with 4-nitrophenyl phosphate as substrate, which is consistent with data published for soluble PhoA. Based on these data we calculated the decoration of pJuFo phagemid with PhoA using the minor and major coat proteins pIII and pVIII as fusion partners under variable inducing conditions. We found that, even if the promoters are fully induced at a concentration of 1000 µM IPTG, the phagemids display maximally one copy of PhoA-Fos-Jun-coat protein fusion, irrespective of whether the protein is presented via pIII or pVIII. However, since PhoA is displayed in a native-like fashion, as deduced from the kinetic parameters of the enzymatic reaction, the pJuFo technology provides a versatile tool for the functional screening of complex cDNA libraries displayed on the phagemids' surface

Display of E. coli Alkaline Phosphatase pIII or pVIII Fusions on Phagemid Surfaces Reveals Monovalent Decoration with Active Molecules

Active alkaline phosphatase of Escherichia coli (PhoA, EC 3.1.3.1) was displayed via the leucine zipper element of the Jun-Fos heterodimer on the surface of filamentous phage and the kinetic parameters Km and kcat were determined. The phoA gene was cloned downstream of fos while jun was inserted upstream of pIII or pVIII, alternatively, in the pJuFo phagemid vector. Both fusion genes are regulated by independent lacZ promoters. PhoA displayed on the phagemid pIII surface exhibited a Km of 11.2 µM with 4-nitrophenyl phosphate as substrate, which is consistent with data published for soluble PhoA. Based on these data we calculated the decoration of pJuFo phagemid with PhoA using the minor and major coat proteins pIII and pVIII as fusion partners under variable inducing conditions. We found that, even if the promoters are fully induced at a concentration of 1000 µM IPTG, the phagemids display maximally one copy of PhoA-Fos-Jun-coat protein fusion, irrespective of whether the protein is presented via pIII or pVIII. However, since PhoA is displayed in a native-like fashion, as deduced from the kinetic parameters of the enzymatic reaction, the pJuFo technology provides a versatile tool for the functional screening of complex cDNA libraries displayed on the phagemids' surface

The misuse of colour in science communication

The accurate representation of data is essential in science communication. However, colour maps that visually distort data through uneven colour gradients or are unreadable to those with colour-vision deficiency remain prevalent in science. These include, but are not limited to, rainbow-like and red–green colour maps. Here, we present a simple guide for the scientific use of colour. We show how scientifically derived colour maps report true data variations, reduce complexity, and are accessible for people with colour-vision deficiencies. We highlight ways for the scientific community to identify and prevent the misuse of colour in science, and call for a proactive step away from colour misuse among the community, publishers, and the press