Les TIC et la réussite éducative au collégial : recherche /

"Cette recherche a été subventionnée par le Ministère de l'enseignement supérieur et de la science dans le cadre du Programme d'aide à la recherche sur l'enseignement et l'apprentissage"Médiagraphie: p. 97-10

Les TIC et la réussite éducative au collégial

"Cette recherche a été subventionnée par le Ministère de l'enseignement supérieur et de la science dans le cadre du Programme d'aide à la recherche sur l'enseignement et l'apprentissage"Comprend des références bibliographique

Impact of systemic enzyme supplementation on low-grade inflammation in humans

Systemic enzyme therapy has been shown to be efficient in treating pain and inflammation associated with injury or musculoskeletal disorders. However, whether systemic enzyme supplementation also attenuates subclinical inflammation remains to be investigated. In this randomized controlled trial, we investigated the impact of systemic enzyme supplementation on inflammatory gene expression as well as on markers of inflammation in 24 adult men and women with subclinical inflammation (serum C-reactive protein [CRP] levels >1 mg/L and <10 mg/L). Participants were supplemented with systemic enzymes (Wobenzym®450 FIP from bromelain and 1440 FIP from trypsin, 6 tablets/d) or placebo for periods of 4 weeks separated by a 4-week washout period. Systemic enzyme supplementation had no impact on expression levels of whole blood cell inflammatory genes compared with placebo but significantly reduced serum IL-6 levels (p = 0.04). However, there was a significant sex × treatment interaction for IL-6 (p = 0.02) and CRP (p = 0.007). Specifically, both serum IL-6 and CRP concentrations were significantly reduced in men (p ≤ 0.03) but not in women (p ≥ 0.08). This study suggests that short-term supplementation with systemic enzymes may attenuate subclinical inflammation, with perhaps greater effects among men than among women

Enseigner l'éducation physique en temps de pandémie : Pratiques enseignantes

Cet article présente les changements apportés aux pratiques enseignantes de Johanne Tremblay, enseignante d’éducation physique et coordonnatrice départementale au cégep de Saint-Félicien, lors de la pandémie de la COVID-19. L’article fait état des différentes adaptations qu’elle a réalisées durant les sessions d’hiver et d’automne 2020. Il met en valeur le rôle et l’apport des principaux acteurs de son milieu, de même que l’importance de la collaboration durant cette période de grand bouleversement

Les TIC et la réussite éducative au collégial

"Cette recherche a été subventionnée par le Ministère de l'enseignement supérieur et de la science dans le cadre du Programme d'aide à la recherche sur l'enseignement et l'apprentissage (PAREA)"Comprend des références bibliographique

Calvarial doughnut lesions with bone fragility in a French-Canadian family; case report and review of the literature

Calvarial Doughnut Lesions with Bone Fragility (CDL) is an autosomal dominant genetic disease, characterized by low bone mineral density, multiple fractures starting in childhood, and sclerotic doughnut-shaped lesions in the cranial bones. Aube and colleagues described in 1988 a French-Canadian family of 12 affected members who had a clinical diagnosis of doughnut lesions of the skull, with pathological fractures, osteopenia, "bone in bone" in the vertebral bodies and squaring of metatarsal and metacarpal bones. Herein we study new members of this family. Sequential genetic testing identified a nonsense variant c.148C>T, p. Arg50* in SGMS2 previously reported in other families. SGMS2 encodes Sphingomyelin Synthase 2, which produces Sphingomyelin (SM), a major lipid component of the plasma membrane that plays a role in bone mineralization. The nonsense variant is associated with milder phenotype. The proband presents with bone in bone vertebral appearance that had been defined uniquely in the first cases described in the same family. The proband's son was identified to carry the same variant, which makes him the sixth generation with the diagnosis of CDL. We also report that the same pathogenic variant was identified in another previously described family, from France. These reports further confirm the genetic basis of CDL, the recurrence of the same variant (p.Arg50*) in individuals of the same ancestry, and the variable penetrance of some of the clinical findings.Peer reviewe

Novel Perfluorinated Triblock Amphiphilic Copolymers for Lipid-Shelled Microbubble Stabilization

International audienceAmphiphilic triblock (Atri) copolymers made of perfluorinated alkyl chain linked to hydrocarbon chain and methoxy-poly(ethylene glycol) of three different molecular weights were synthesized. In vitro evaluation demonstrated that these new compounds were noncytotoxic. Characterization and interaction of each triblock copolymer with a branched polyamine myristoyl lipid (2-{3[bis-(3-amino-propyl)-amino]-propylamino}-N-ditetradecyl carbamoyl methyl-acetamide, DMAPAP) were studied by the Langmuir film method and thermal analysis. The triblock copolymer/cationic lipids (1:10, w/w) were mixed with perfluorobutane gas to form microbubbles (MBs). The latter were characterized by optical microscopy to get the microbubble size and concentration by densimetry to determine the amount of encapsulated gas and by ultrasound to assess oscillation properties. Atri with the lowest and intermediate weights were shown to interact with the cationic lipid DMAPAP and stabilize the Langmuir film. In that case, monodisperse microbubbles ranging from 2.3 ± 0.1 to 2.8 ± 0.1 μm were obtained. The proportion of encapsulated gas within the MB shell increased up to 3 times after the incorporation of the copolymer with the lowest and intermediate weights. Moreover, the acoustic response of the microbubbles was maintained in the presence of the copolymers

Cationic microbubbles and antibiotic-free miniplasmid for sustained ultrasound–mediated transgene expression in liver

International audienceDespite the increasing number of clinical trials in gene therapy, no ideal methods still allow non-viral gene transfer in deep tissues such as the liver. We were interested in ultrasound (US)-mediated gene delivery to provide long term liver expression. For this purpose, new positively charged microbubbles were designed and complexed with pFAR4, a highly efficient small length miniplasmid DNA devoid of antibiotic resistance sequence. Sonoporation parameters, such as insonation time, acoustic pressure and duration of plasmid injection were controlled under ultrasound imaging guidance. The optimization of these various parameters was performed by bioluminescence optical imaging of luciferase reporter gene expression in the liver. Mice were injected with 50 μg pFAR4-LUC either alone, or complexed with positively charged microbubbles, or co-injected with neutral MicroMarker™ microbubbles, followed by low ultrasound energy application to the liver. Injection of the pFAR4 encoding luciferase alone led to a transient transgene expression that lasted only for two days. The significant luciferase signal obtained with neutral microbubbles decreased over 2 days and reached a plateau with a level around 1 log above the signal obtained with pFAR4 alone. With the newly designed positively charged microbubbles, we obtained a much stronger bioluminescence signal which increased over 2 days. The 12-fold difference (p < 0.05) between MicroMarker™ and our positively charged microbubbles was maintained over a period of 6 months. Noteworthy, the positively charged microbubbles led to an improvement of 180-fold (p < 0.001) as regard to free pDNA using unfocused ultrasound performed at clinically tolerated ultrasound amplitude. Transient liver damage was observed when using the cationic microbubble-pFAR4 complexes and the optimized sonoporation parameters. Immunohistochemistry analyses were performed to determine the nature of cells transfected. The pFAR4 miniplasmid complexed with cationic microbubbles allowed to transfect mostly hepatocytes compared to its co-injection with MicroMarker™ which transfected more preferentially endothelial cells