Estrategias bioanalíticas en estudios metalómicos de fármacos de platino

En este artículo se muestra el potencial que presentan las modernas estrategias bioanalíticas en estudios metalómicos de fármacos de platino. La combinación de técnicas de separación multidimensionales cromatográficas y/o electroforéticas con la espectrometría de masas atómica ICP-MS y molecular ESI-MS/MS se presenta como una valiosa alternativa en este tipo de estudios

The FXII c.-4T > C Polymorphism as a Disease Modifier in Patients With Hereditary Angioedema Due to the FXII p.Thr328Lys Variant

Background: Hereditary angioedema due to the Thr328Lys variant in the coagulation factor XII (HAE-FXII) affects mainly women in whom the symptomatology is dependent on high estrogen levels. Clinical variability and incomplete penetrance are challenging features that hinder the diagnosis and management of HAE-FXII. The c.-4T>C Kozak polymorphism is the only common variation accounting for FXII plasma levels and was previously shown to modify the course of HAE due to C1-Inhibitor deficiency. Objectives: To assess the influence of the c.-4T>C polymorphism on disease expression in 39 Spanish HAE-FXII index patients. Methods: The c.-4T>C polymorphism was sequenced by the standard Sanger method, and HAE severity was calculated according to the score by Cumming et al. (2003) The activation of the contact system was quantified by the kallikrein-like activity of plasma in chromogenic assays upon activation with high-molecular-weight dextran sulfate. Results: The c.-4CC genotype was overrepresented in the studied cohort: 82% were CC-homozygous (expected frequency = 59%) and 18% were CT-heterozygous (expected frequency = 39%) (p = 0.001). Patients with a c.-4CC genotype exhibited higher kallikrein-like activity (0.9659 +/- 0.1136) than those with a c.-4TC genotype (0.7645 +/- 0.1235) (p = 0.024) or healthy donors. Moreover, the polymorphism influenced HAE-FXII severity score (c.-4CC = 4.43 +/- 2.28 vs c.-4TC = 2.0 +/- 1.15; p = 0.006) but not the degree of estrogen dependence or time until remission. Conclusion: The c.-4T>C polymorphism is overrepresented in a Spanish HAE-FXII cohort and significantly influences the degree of contact system activation and the clinical severity of the disease

Modulating charge carrier density and mobility in doped graphene by covalent functionalization

[EN] Covalent B-functionalization of B-doped graphene has been performed for the first time. The electronic properties and Hall effect of functionalized N- and B-doped graphene can be tuned by tailoring the electron-donating/-withdrawing properties of the organic addend.The authors appreciate support from the Ministerio de Economia y Competitividad (MINECO) of Spain (projects CTQ2015-69153-CO2-1, CTQ2016-79189-R and MAT2015-69669-P) and the Junta de Comunidades de Castilla-La Mancha (project SBPLY/17/180501/000254). L. M. A. thanks MINECO (CTQ2016-79189-R) for a doctoral FPI grant.Arellano, LM.; Yue, S.; Atienzar Corvillo, PE.; Gómez-Escalonilla, MJ.; Ortega Higueruelo, FJ.; Fierro, JLG.; García Gómez, H.... (2019). Modulating charge carrier density and mobility in doped graphene by covalent functionalization. Chemical Communications. 55(67):9999-10002. https://doi.org/10.1039/c9cc04571fS9999100025567Wang, X., Sun, G., Routh, P., Kim, D.-H., Huang, W., & Chen, P. (2014). Heteroatom-doped graphene materials: syntheses, properties and applications. Chem. Soc. Rev., 43(20), 7067-7098. doi:10.1039/c4cs00141aLavorato, C., Primo, A., Molinari, R., & Garcia, H. (2013). N-Doped Graphene Derived from Biomass as a Visible-Light Photocatalyst for Hydrogen Generation from Water/Methanol Mixtures. Chemistry - A European Journal, 20(1), 187-194. doi:10.1002/chem.201303689Latorre-Sánchez, M., Primo, A., & García, H. (2013). P-Doped Graphene Obtained by Pyrolysis of Modified Alginate as a Photocatalyst for Hydrogen Generation from Water-Methanol Mixtures. Angewandte Chemie International Edition, 52(45), 11813-11816. doi:10.1002/anie.201304505Duan, J., Chen, S., Jaroniec, M., & Qiao, S. Z. (2015). Heteroatom-Doped Graphene-Based Materials for Energy-Relevant Electrocatalytic Processes. ACS Catalysis, 5(9), 5207-5234. doi:10.1021/acscatal.5b00991Putri, L. K., Ong, W.-J., Chang, W. S., & Chai, S.-P. (2015). Heteroatom doped graphene in photocatalysis: A review. Applied Surface Science, 358, 2-14. doi:10.1016/j.apsusc.2015.08.177Niu, L., Li, Z., Hong, W., Sun, J., Wang, Z., Ma, L., … Yang, S. (2013). Pyrolytic synthesis of boron-doped graphene and its application as electrode material for supercapacitors. Electrochimica Acta, 108, 666-673. doi:10.1016/j.electacta.2013.07.025Sahoo, M., Sreena, K. P., Vinayan, B. P., & Ramaprabhu, S. (2015). Green synthesis of boron doped graphene and its application as high performance anode material in Li ion battery. Materials Research Bulletin, 61, 383-390. doi:10.1016/j.materresbull.2014.10.049Khai, T. V., Na, H. G., Kwak, D. S., Kwon, Y. J., Ham, H., Shim, K. B., & Kim, H. W. (2012). Comparison study of structural and optical properties of boron-doped and undoped graphene oxide films. Chemical Engineering Journal, 211-212, 369-377. doi:10.1016/j.cej.2012.09.081Osumi, S., Saito, S., Dou, C., Matsuo, K., Kume, K., Yoshikawa, H., … Yamaguchi, S. (2016). Boron-doped nanographene: Lewis acidity, redox properties, and battery electrode performance. Chemical Science, 7(1), 219-227. doi:10.1039/c5sc02246kXu, Q., Jiang, X., Zhu, W., Chen, C., Hu, G., & Li, Q. (2016). Synthesis, preliminary biological evaluation and 3D-QSAR study of novel 1,5-disubstituted-2(1H)-pyridone derivatives as potential anti-lung cancer agents. Arabian Journal of Chemistry, 9(5), 721-735. doi:10.1016/j.arabjc.2015.08.001Pimenta, M. A., Dresselhaus, G., Dresselhaus, M. S., Cançado, L. G., Jorio, A., & Saito, R. (2007). Studying disorder in graphite-based systems by Raman spectroscopy. Phys. Chem. Chem. Phys., 9(11), 1276-1290. doi:10.1039/b613962kVoggu, R., Rout, C. S., Franklin, A. D., Fisher, T. S., & Rao, C. N. R. (2008). Extraordinary Sensitivity of the Electronic Structure and Properties of Single-Walled Carbon Nanotubes to Molecular Charge-Transfer. The Journal of Physical Chemistry C, 112(34), 13053-13056. doi:10.1021/jp805136eBiswal, M., Zhang, X., Schilter, D., Lee, T. K., Hwang, D. Y., Saxena, M., … Ruoff, R. S. (2017). Sodide and Organic Halides Effect Covalent Functionalization of Single-Layer and Bilayer Graphene. Journal of the American Chemical Society, 139(11), 4202-4210. doi:10.1021/jacs.7b00932Vizuete, M., Gómez-Escalonilla, M. J., Fierro, J. L. G., Ohkubo, K., Fukuzumi, S., Yudasaka, M., … Langa, F. (2014). Photoinduced electron transfer in a carbon nanohorn–C60 conjugate. Chemical Science, 5(5), 2072. doi:10.1039/c3sc53342eAllongue, P., Delamar, M., Desbat, B., Fagebaume, O., Hitmi, R., Pinson, J., & Savéant, J.-M. (1997). Covalent Modification of Carbon Surfaces by Aryl Radicals Generated from the Electrochemical Reduction of Diazonium Salts. Journal of the American Chemical Society, 119(1), 201-207. doi:10.1021/ja963354sBarrejón, M., Gómez-Escalonilla, M. J., Fierro, J. L. G., Prieto, P., Carrillo, J. R., Rodríguez, A. M., … Langa, F. (2016). Modulation of the exfoliated graphene work function through cycloaddition of nitrile imines. Physical Chemistry Chemical Physics, 18(42), 29582-29590. doi:10.1039/c6cp05285aBarrejón, M., Primo, A., Gómez-Escalonilla, M. J., Fierro, J. L. G., García, H., & Langa, F. (2015). Covalent functionalization of N-doped graphene by N-alkylation. Chemical Communications, 51(95), 16916-16919. doi:10.1039/c5cc06285cSu, C.-Y., Xu, Y., Zhang, W., Zhao, J., Tang, X., Tsai, C.-H., & Li, L.-J. (2009). Electrical and Spectroscopic Characterizations of Ultra-Large Reduced Graphene Oxide Monolayers. Chemistry of Materials, 21(23), 5674-5680. doi:10.1021/cm902182

Revista de Vertebrados de la Estación Biológica de Doñana

Página 298 con error de impresiónEstudio cariológico en dos especies de Serránidos del Mediterráneo (Peces: PerciformesRelaciones morfométricas de Atherina boyeri Risso (Pisces: Atherinidae) de la laguna de Zoñar (Córdoba, España)Contribución al conocimiento de la biometríay osteología de Barbus barbus bocagei, Steindachner, 1866 (Pisces: CyprinidaeLa actividad de la salamandra, Salamandra salamandra (L.), en Galicia.Estudios sobre el sapo corredor (Bufo calamita) en el Sur de España.1. BiometríaEstudios sobre el sapo corredor (Bufo calamita) en el Sur de España. II. AlimentaciónBiología de la reproducción de Rana iberica Boulenger 1879 en zonas simpátridas con Rana temporaria Linneo, 1758Nuevos datos sobre la distribución geográfica de Lacerta monticola cantabrica Mertens, 1929. (Sauria, lacertidae).Datos sobre Lacerta monticola Boulenger, 1905 (Saurio: lacertidae)en el oeste del Sistema Central.Nueva especie de Anolis (lacertilia, Iguanidae) para CubaEtograma cuantificado del cortejo en Falco naumannOntogénesis del comportamiento predador en Falco naumanniContaminación xenobiótica del Parque Nacional de Doñana. 1. Residuos de insecticidas organoclorados, bifenilos policlorados y mercurio en anseriformes y gruiformesReproducción del críalo (Clamator glandarius) en Sierra Morena CentraNidificación de Picus viridis en taludes de arcilla en Ramblas de Guadix (Granada)Comportamiento del calamón Porphyrio porphyrio (Linnaeus, 1758) en Doñana, Marismas del GuadalquiviBiología y ecología de la malvasía (Oxyura leucocephala) en Andalucía.On the differential diet of Carnivora in islands:a method for analysing it and a particular case.Notas sobre la distribución pasada y actual del meloncillo Herpestes ichneumon (L.) en la Península IbéricaEstructuración de las interacciones en una camada de lobos (Canís lupus)Nuevos datos sobre la distribución del Cottus gobio L. (pisces, cottidae) en EspañaSobre la alimentación de Callopistes maculatus (Reptilia,teiidaeObservación de Lacerta lepida depredando un nido de Alectoris rufaNueva cita del galápago leproso Mauremys leprosa (Scheigger, 1812) en los pirineosPrimera cita de Psammodromus hispanicus (Fitzinger) para GaliciaSobre la presencia de Gallotia (=Lacerta) atlantica (Peters y Doria, 1882) en Gran CanariaNota sobre las Lacerta monticola Boulenger, 1905 de las zonas del norte de GaliciaPrimeras notas herpetológicas de la provincia de Soria.Datos sobre selección de hábitat y ecología alimenticia del porrón pardo (Aythya nyroca)Probable nueva área de cría del pechiazul (Luscinia svecica cyanecula) en el sistema central. PerisPredación de Falco peregrinus y Falco subbuteo sobre quirópterosResultados de la producción de Oxyura leucocephala en el año 1981 en las lagunas de Zóñar y el rincónAnálisis de la dieta de Tyto alba en un medio árido antropógeno de los alrededores de Almería¿Son Eudocimus ruber y E. albus distintas especies?EL Estornino pinto (Sturnus vulgaris) en Canarias: nueva especie nidifiante en el archipiélagoDatos sobre la alimentación otoñal del cárabo (Strix aluco) en la sierra de CádizObservación primaveral de rapaces y otras aves en el páramo del estado de Mérida (Venezuela).Murciélago hematófago (Desmodus rotundus) parasitando a un chigüire (Hidrochoerus hydrochaeris)Observaciones sobre la reproducción del zacatuche o teporinho Romerolagus diazi (Mammalia: lagomorpha)Estudio electroforético de hemoglobinas y esterasas sanguíneas en Rhinolophus ferrumequinum (Chiroptera: rhinolophidae) y de hemoglobinas en Tadaria taeniotis (chiroptera: molossidae)Peer reviewe

Acidic Urine pH and Clinical Outcome of Lower Urinary Tract Infection in Kidney Transplant Recipients Treated with Ciprofloxacin and Fosfomycin

Different factors, including antimicrobial resistance, may diminish the effectiveness of antibiotic therapy, challenging the management of post-transplant urinary tract infection (UTI). The association of acidic urine pH with microbiological and clinical outcomes was evaluated after fosfomycin or ciprofloxacin therapy in 184 kidney transplant recipients (KTRs) with UTI episodes by Escherichia coli (N = 115) and Klebsiella pneumoniae (N = 69). Initial urine pH, antimicrobial therapy, and clinical and microbiological outcomes, and one- and six-month follow-up were assessed. Fosfomycin was prescribed in 88 (76.5%) E. coli and 46 (66.7%) K. pneumoniae UTI episodes in the total cohort. When the urine pH ≤ 6, fosfomycin was prescribed in 60 (52.2%) E. coli and 29 (42.0%) K. pneumoniae. Initial urine pH ≤ 6 in E. coli UTI was associated with symptomatic episodes (8/60 vs. 0/55, p = 0.04) at one-month follow-up, with a similar trend in those patients receiving fosfomycin (7/47 vs. 0/41, p = 0.09). Acidic urine pH was not associated with microbiological or clinical cure in K. pneumoniae UTI. At pH 5, the ciprofloxacin MIC90 increased from 8 to >8 mg/L in E. coli and from 4 to >8 mg/L in K. pneumoniae. At pH 5, the fosfomycin MIC90 decreased from 8 to 4 mg/L in E. coli and from 512 to 128 mg/L in K. pneumoniae. Acidic urine is not associated with the microbiological efficacy of fosfomycin and ciprofloxacin in KTRs with UTI, but it is associated with symptomatic UTI episodes at one-month follow-up in E. coli episodes.This study has been funded by Instituto de Salud Carlos III, through the projects PI17/01405 and PI20/01255, by the Subdirección General de Evaluación y Fomento de la Investigación, Ministerio de Economía, Industria y Competitividad, US-1381501 US/JUNTA/FEDER, UE, by the Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, and the Spanish Network for Research in Infectious Diseases (REIPI, RD16/0016/0009) and co-funded by the European Union.Peer reviewe